Article
Biology
Achinta Sannigrahi, Sourav Chowdhury, Bidisha Das, Amrita Banerjee, Animesh Halder, Amaresh Kumar, Mohammed Saleem, Athi N. Naganathan, Sanat Karmakar, Krishnananda Chattopadhyay
Summary: Research shows that the stability and aggregation behavior of SOD1 in membrane environment are affected by Zn, not Cu. Two loop regions play a role in membrane attachment of SOD1 and aggregation driven by lipid-induced conformational changes.
Article
Biochemistry & Molecular Biology
Yoshiaki Furukawa, Atsuko Shintani, Teppei Kokubo
Summary: The study demonstrates that the disulfide-reduced SodC can secure copper and zinc ions through thiolate groups, and binds cuprous and cupric ions more tightly than SodC with the disulfide bond, leading to the production of enzymatically active SodC.
Article
Biochemistry & Molecular Biology
Kenji Watanabe, Shuichi Shibuya, Yusuke Ozawa, Toshihiko Toda, Takahiko Shimizu
Summary: The study showed that p53 regulated ROS-mediated apoptotic cell death and tumorigenesis, but did not affect ROS-mediated tissue degeneration in SOD-deficient models.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, Research & Experimental
Ping Lu, Hua-Juan Yan, Cui Yang, Wen-Cai Feng, Fei Hu, Yue-Ying Wu, Wei-Wen Sun, Mei-Mei Gao, Yue-Sheng Long
Summary: The research suggests that high fat consumption leads to the generation of reactive oxygen species, decreases copper/zinc superoxide dismutase activity, and promotes neurodegeneration. Additionally, it was shown that saturated fatty acid palmitic acid decreases copper chaperone CCS levels, potentially leading to a decrease in SOD1 activity.
Article
Biochemistry & Molecular Biology
Edurne Tellechea, Aaron C. Asensio, Paula Ciaurriz, Javier Buezo, Pedro Lopez-Gomez, Marina Urra, Jose F. Moran
Summary: This study investigates the characteristics of the recombinant cowpea FeSOD immobilized onto gold nanoparticles (AuNPs) and the effects of NP surface chemistry and biofunctionalization methods on enzyme activity. The results highlight the importance of the biomolecule and AuNP interaction for the functionality of the hybrid and demonstrate the potential of these strategies in biomedical applications.
Article
Biology
ShylajaNaciyar Mohandass, Mangalalakshmi Ragavan, Dineshbabu Gnanasekaran, Uma Lakshmanan, Prabaharan Dharmar, Sushanta Kumar Saha
Summary: Synthetic azo dyes are widely used but their improper discharge poses a threat to the environment. Genetically modified cyanobacteria with enhanced H2O2 accumulation through SOD overexpression can effectively degrade these dyes, suggesting a valuable bioremediation strategy.
Article
Chemistry, Multidisciplinary
Sunil Kumar Vimal, Hongyi Cao, Amit Dubey, Lokesh Agrawal, Nishit Pathak, Hua Zuo, Deepak Kumar, Sanjib Bhattacharyya
Summary: This study evaluated the pharmacological potential of a synthesized Au-PEG nanoconjugate in a transgenic mouse model of ALS. The results showed that Au-PEG treatment extended survival, delayed disease onset, and improved motor performance. Furthermore, molecular docking studies revealed that the addition of gold nanoparticles enhanced the binding affinity of kinesin to tubulin, restoring motor function.
NEW JOURNAL OF CHEMISTRY
(2022)
Article
Chemistry, Physical
Qi-Wen Chen, Meng-Wei Cao, Ji-Yan Qiao, Qian-Ru Li, Xian-Zheng Zhang
Summary: An integrated cascade catalysis for anti-inflammation therapy was developed by decorating catalase-biomimetic ceria nanoparticles onto the Spirulina platensis surface. The resulting biohybrids exhibit excellent catalytic competence for transforming superoxide anion radicals to hydrogen peroxide and subsequently catalyzing its disproportionation. The biohybrids show promising therapeutic effects in ulcerative colitis and Crohn's disease, highlighting their potential for enzyme-based biomedical applications.
NANOSCALE HORIZONS
(2023)
Article
Multidisciplinary Sciences
Tania M. Manieri, Stefano L. Sensi, Rosanna Squitti, Giselle Cerchiaro
Summary: The study altered the activity of SOD1 in AD patients by modifying its zinc complexation capacity, leading to decreased enzymatic activity even when complexed with copper. The mutant SOD1 presented instability in zinc and copper coordination, making it a valuable in vitro model for studying SOD1 behavior in AD patients.
Article
Multidisciplinary Sciences
Liangzhong Lim, Jian Kang, Jianxing Song
Summary: This study reveals the role of cations in the initial folding of hSOD1. Most cations, including Zn2+ mimics, showed negligible binding or induction for folding of nascent hSOD1. Cu2+ exhibited extensive binding to the unfolded state but led to severe aggregation. Surprisingly, Fe2+ was found to have Zn2+-like folding-inducing capacity.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Danica Tasic, Milos Opacic, Sanja Kovacevic, Aleksandra Nikolic Kokic, Milena Dimitrijevic, Dusan Nikolic, Danijela Vojnovic Milutinovic, Dusko Blagojevic, Ana Djordjevic, Jelena Brkljacic
Summary: The effects of a fructose-rich diet and chronic stress on copper metabolism in the kidneys are still understudied. This study found that fructose, independent of stress, decreased renal copper level and modulated renal copper metabolism to preserve vital cellular function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Clinical Neurology
Shlomit Ezer, Muhannad Daana, Julien H. Park, Shira Yanovsky-Dagan, Ulrika Nordstrom, Adily Basal, Simon Edvardson, Ann Saada, Markus Otto, Vardiella Meiner, Stefan L. Marklund, Peter Munch Andersen, Tamar Harel
Summary: Pathogenic variants in the SOD1 gene are associated with a severe motor-neurological syndrome in infants, characterized by global developmental delay and movement impairments. This study identified a homozygous loss-of-function variant in the SOD1 gene in an infant with severe neurological symptoms. Further analysis showed that this variant leads to instability and degeneration of the SOD1 protein. The study highlights the importance of specific valine residues in the SOD1 protein and suggests implications for future therapeutic research.
Article
Clinical Neurology
Delia Gagliardi, Paolo Ripellino, Megi Meneri, Roberto Del Bo, Sara Antognozzi, Giacomo Pietro Comi, Claudio Gobbi, Antonia Ratti, Nicola Ticozzi, Vincenzo Silani, Dario Ronchi, Stefania Corti
Summary: In this study, the authors provided a clinical and molecular description of a cohort of SOD1-ALS patients, revealing the heterogeneity in clinical and molecular characteristics of SOD1 mutations. The cohort exhibited variable expressivity, atypical presentations, and different modes of inheritance. With the availability of SOD1-directed antisense oligonucleotide for SOD1-ALS patients, prompt screening for SOD1 mutations in ALS patients is recommended.
FRONTIERS IN NEUROLOGY
(2023)
Review
Chemistry, Multidisciplinary
Benjamin G. Trist, James B. Hilton, Dominic J. Hare, Peter J. Crouch, Kay L. Double
Summary: Cu/Zn superoxide dismutase (SOD1) is a crucial antioxidant enzyme in eukaryotic life, with mitochondrial aerobic respiration increasing cellular superoxide production and reliance on SOD1. Diseases of the central nervous system often involve disruptions in SOD1 biology, revealing complex molecular characteristics that differentiate healthy from disease-contributing SOD1 proteins. This review highlights current knowledge of SOD1 biology, from genetics to protein function and stability.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Plant Sciences
Giovanna Salbitani, Angela Perrone, Luigi Rosati, Carmen Laezza, Simona Carfagna
Summary: This study investigates the effects of sulfur deprivation on Galdieria sulphuraria and reveals that it leads to a decrease in total protein content and an increase in soluble protein fraction. The glutathione content in Galdieria sulphuraria remains high even under sulfur deprivation.
Review
Biochemistry & Molecular Biology
Stefanie D. Boyd, Morgan S. Ullrich, Amelie Skopp, Duane D. Winkler
Article
Multidisciplinary Sciences
Fabian C. Herbert, Sameera S. Abeyrathna, Nisansala S. Abeyrathna, Yalini H. Wijesundara, Olivia R. Brohlin, Francesco Carraro, Heinz Amenitsch, Paolo Falcaro, Michael A. Luzuriaga, Alejandra Durand-Silva, Shashini D. Diwakara, Ronald A. Smaldone, Gabriele Meloni, Jeremiah J. Gassensmith
Summary: This study demonstrates the successful generation and immobilization of metastable lipid, protein-detergent, and protein-lipid supramolecular complexes within a zeolitic-imidazole framework (ZIF) to enhance stability against various stressors. The immobilized complexes exhibit resistance to elevated temperatures, chemical denaturants, aging, and mechanical stresses, maintaining their native morphology, structure, and activity. Immobilizing lipid nanoparticles and lipid-protein assemblies remains challenging due to lipid dynamics and protein instability, but this work shows promise for long-term storage at room temperature.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Jenifer S. Calvo, Rhiza Lyne E. Villones, Nicholas J. York, Ewelina Stefaniak, Grace E. Hamilton, Allison L. Stelling, Wojciech Bal, Brad S. Pierce, Gabriele Meloni
Summary: Researchers have identified and characterized transient radical intermediates formed in the reaction between Zn7MT-3 and Cu(II). These intermediates are stable and have long lifetimes, providing insights into the assembly process of the MT-3 Cu(I)(4)-Cys(5) cluster.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biology
Christina Gronberg, Qiaoxia Hu, Dhani Ram Mahato, Elena Longhin, Nina Salustros, Annette Duelli, Pin Lyu, Viktoria Bagenholm, Jonas Eriksson, Komal Umashankar Rao, Domhnall Iain Henderson, Gabriele Meloni, Magnus Andersson, Tristan Croll, Gabriela Godaly, Kaituo Wang, Pontus Gourdon
Summary: This study provides structural insights into PIB-4-ATPases, revealing unique metal transport pathways and identifying two compounds that can kill bacteria. Understanding how these proteins work may lead to the development of plants capable of removing heavy metals from contaminated soils and the discovery of new antibiotic compounds.
Article
Biochemistry & Molecular Biology
Li Liu, Xander E. Wilcox, Andrew J. Fisher, Stefanie D. Boyd, Jiahe Zhi, Duane D. Winkler, Lee A. Bulla
Summary: The G-protein-coupled receptor BT-R-1 in the moth Manduca sexta binds tightly to Cry1A toxins and triggers a signaling pathway leading to insect death. The specific binding site on BT-R-1, known as the toxin-binding site (TBS), contains a highly conserved 94-amino acid polypeptide that competitively inhibits the insecticidal activity of Cry1A toxins. The study demonstrates the exclusive involvement of BT-R-1 in initiating insect cell death and reveals that BT-R-1 contains a single TBS.
Article
Biochemistry & Molecular Biology
Ping Li, Niloofar Nayeri, Kamil Gorecki, Eva Ramos Becares, Kaituo Wang, Dhani Ram Mahato, Magnus Andersson, Sameera S. Abeyrathna, Karin Lindkvist-Petersson, Gabriele Meloni, Julie Winkel Missel, Pontus Gourdon
Summary: The study characterizes the structure and function of the outer membrane component PcoB in the Pco system, revealing its role in ion-conducting pathway and a potential unorthodox defense mechanism against metal stress.
Article
Multidisciplinary Sciences
Ping Li, Amber L. Hendricks, Yong Wang, Rhiza Lyne E. Villones, Karin Lindkvist-Petersson, Gabriele Meloni, J. A. Cowan, Kaituo Wang, Pontus Gourdon
Summary: This study utilizes cryo-EM to reveal the structure of Atm1 transporter and proposes its molecular mechanism in the maturation of iron-sulfur proteins. These findings are of significant importance for understanding the transport mechanism of eukaryotic ABCB7-type proteins.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Nina Salustros, Christina Gronberg, Nisansala S. Abeyrathna, Pin Lyu, Fredrik Oradd, Kaituo Wang, Magnus Andersson, Gabriele Meloni, Pontus Gourdon
Summary: The authors report the molecular structure of CopA, a copper-transporting P-1B-ATPase in an inward-facing E1 conformation. The findings provide insights into the mechanism of copper transport and are likely applicable to human P-1B members.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Sameera S. Abeyrathna, Nisansala S. Abeyrathna, Priyanka Basak, Gordon W. Irvine, Limei Zhang, Gabriele Meloni
Summary: Transmembrane P-1B-type ATPase pumps are crucial for maintaining cellular metal homeostasis and detoxification. We developed a platform to study metal selectivity, translocation events, and transport mechanism in real-time using fluorescent sensors and X-ray absorption spectroscopy. Our findings demonstrate that Zn(ii)-pumps are electrogenic uniporters with promiscuous coordination plasticity.
Article
Chemistry, Multidisciplinary
Weicheng Peng, Caden C. Maydew, Hiu Kam, Jacob K. Lynd, Jasmine N. Tutol, Shelby M. Phelps, Sameera Abeyrathna, Gabriele Meloni, Sheel C. Dodani
Summary: Chloride is crucial for all forms of life and recent research has demonstrated the potential of chloride-sensitive fluorescent proteins for cellular applications. This study presents a bioinformatics strategy to efficiently identify and design new chloride-sensitive fluorescent proteins, using GFPxm163 as an example.
Article
Biochemistry & Molecular Biology
Anjala W. Bulathge, Rhiza Lyne E. Villones, Fabian C. Herbert, Jeremiah J. Gassensmith, Gabriele Meloni
Summary: This study investigated the preferential reactivity of cisplatin towards human Zn7MT-2, MTF-1 zinc fingers, and the entire MTF-1 zinc finger domain through in vitro competition reactions. The results demonstrated that cisplatin preferentially reacts with Zn7MT-2, releasing zinc and triggering MTF-1 activation and binding to specific DNA sequences.
Article
Chemistry, Multidisciplinary
Matthew R. Mehlenbacher, Rahma Elsiesy, Rabina Lakha, Rhiza Lyne E. Villones, Marina Orman, Christina L. Vizcarra, Gabriele Meloni, Dean E. Wilcox, Rachel N. Austin
Summary: This study quantitatively analyzed the Zn2+ and Cu+ binding to mammalian metallothionein-3 (MT-3) using isothermal titration calorimetry (ITC) at pH 7.4, revealing the thermodynamics of their binding. The results showed that Cu+ displaces Zn2+ to form Cu-4(+)-thiolate clusters in both proteins.
Article
Chemistry, Multidisciplinary
Jasmine N. Tutol, Jessica Lee, Hsichuan Chi, Farah N. Faizuddin, Sameera S. Abeyrathna, Qin Zhou, Faruck Morcos, Gabriele Meloni, Sheel C. Dodani
Summary: The power of protein engineering was demonstrated by converting the fluorescent proton-pumping rhodopsin GR into GR1, a red-shifted, turn-on fluorescent sensor for chloride. GR1 provides a pH-dependent response in detergent micelles and live Escherichia coli, offering a reversible, ratiometric readout of changes in extracellular chloride.
Article
Chemistry, Multidisciplinary
Candace E. Benjamin, Zhuo Chen, Olivia R. Brohlin, Hamilton Lee, Arezoo Shahrivarkevishahi, Stefanie Boyd, Duane D. Winkler, Jeremiah J. Gassensmith
Article
Chemistry, Multidisciplinary
Jenifer S. Calvo, Rhiza Lyne E. Villones, Nicholas J. York, Ewelina Stefaniak, Grace E. Hamilton, Allison L. Stelling, Wojciech Bal, Brad S. Pierce, Gabriele Meloni
Summary: MT-3 protein reduces Cu(II) to Cu(I) and forms a multi-nuclear Cu(I)(4)-Cys(5-6) cluster, generating long-lived and oxygen-stable disulfide radical anion intermediates.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)