Article
Biochemistry & Molecular Biology
Paul Batty, Christoph C. H. Langer, Zsuzsanna Takacs, Wen Tang, Claudia Blaukopf, Jan-Michael Peters, Daniel W. Gerlich
Summary: Genetic information is stored in folded linear DNA molecules inside cells. During cell division, sister chromatids need to be disentangled and condensed into separate bodies for proper separation. In human cells, sister chromatids are extensively resolved during interphase, depending on the loop-extruding activity of cohesin. Increasing cohesin's looping capability can further enhance sister DNA resolution, even in the absence of mitosis-specific activities.
Article
Cell Biology
Masashi Nambu, Atsuki Kishikawa, Takatomi Yamada, Kento Ichikawa, Yunosuke Kira, Yuta Itabashi, Akira Honda, Kohei Yamada, Hiroshi Murakami, Ayumu Yamamoto
Summary: In this study, a novel method for directly evaluating kinetochore associations on intact chromosomes in live S. pombe cells was developed. The results showed that sister kinetochores and centromere cores were separately positioned on mitotic chromosomes but associated with each other on meiosis I chromosomes. Kinetochore association depended on meiotic cohesin and cohesin regulators, and mating-pheromone signaling was required for its establishment.
JOURNAL OF CELL SCIENCE
(2022)
Review
Cell Biology
Fioranna Renda, Alexey Khodjakov
Summary: The kinetochore plays a crucial role in facilitating chromosome segregation during cell division by attaching chromosomes to spindle microtubules. It also acts as a signaling hub to regulate cell cycle progression and ensures the accuracy of chromosome segregation. Despite being large and robust molecular assemblies, kinetochores undergo dynamic structural and organizational changes throughout the cell cycle.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Xue Bessie Su, Menglu Wang, Claudia Schaffner, Olga O. Nerusheva, Dean Clift, Christos Spanos, David A. Kelly, Michael Tatham, Andreas Wallek, Yehui Wu, Juri Rappsilber, A. Arockia Jeyaprakash, Zuzana Storchova, Ronald T. Hay, Adele L. Marston
Summary: During cell mitosis, SUMOylation plays a critical role in modulating error correction mechanisms by regulating protein interactions, leading to stable sister kinetochore biorientation and timely anaphase onset.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Sunbin Deng, Jiaxi Cai, Stephen C. Harrison, Huilin Zhou, Stephen M. Hinshaw
Summary: Successful mitosis relies on the correct attachment of chromosomes to microtubules. The kinetochore plays a crucial role in this process by recognizing specific chromatin and ensuring the formation of only one kinetochore per chromatid at the correct location. The crystal structure of the Cse4 peptide bound to the inner kinetochore Okp1-Ame1 complex provides insights into centromere recognition and the organization of the inner kinetochore.
Article
Oncology
Radhika Koranne, Kayla Brown, Hannah Vandenbroek, William R. R. Taylor
Summary: C9ORF78 is a poorly characterized protein found in diverse eukaryotes. It has been suggested to be involved in growth regulatory pathways in malignant tissues. This study discovers a potential function of regulating the spliceosome, new subcellular localization in nuclei and association with kinetochores/centromeres in mitotic cells.
EXPERIMENTAL CELL RESEARCH
(2022)
Article
Plant Sciences
Yang Liu, Chunhui Wang, Handong Su, James A. Birchler, Fangpu Han
Summary: Haspin regulates chromosomal localization and segregation in both human and plant cells through H3T3 phosphorylation, with slightly different roles observed in mitosis and meiosis in plants. Plant Haspin lacks motifs necessary for binding to specific proteins involved in cohesion, leading to distinct mechanisms of action compared to animals.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Multidisciplinary Sciences
Lingluo Chu, Zheng Zhang, Maria Mukhina, Denise Zickler, Nancy Kleckner
Summary: This study reveals the crucial role of interaxis bridges in sister chromatid separation during mitosis. The three-stage pathway of sister chromatid separation is described, with the interaxis bridges playing a prominent role. The findings suggest that bridges are not only removed during anaphase but actively ensure smooth and synchronous sister separation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Ho-Soo Lee, Sunwoo Min, Ye-Eun Jung, Sunyoung Chae, June Heo, Jae-Ho Lee, TaeSoo Kim, Ho-Chul Kang, Makoto Nakanish, Sun-Shin Cha, Hyeseong Cho
Summary: The study reveals that the RSF1-PLK1 axis plays a crucial role in the activation of Aurora B, enhancing its catalytic activity through phosphorylation and promoting chromosome alignment and segregation. This kinase combination is crucial for regulating dynamic microtubule-kinetochore attachment in early mitosis.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
M. Johansson, Y. Azuma, D. J. Clarke
Summary: DNA topoisomerase II (TopoII) plays a crucial role in mitosis by decatenating chromosomes. When the TopoII enzyme cycle stalls due to failed ATP hydrolysis, the onset of anaphase is delayed to ensure accurate chromosome segregation. Recent evidence shows that the TopoII checkpoint response requires Aurora B and Haspin kinases, and is triggered by SUMOylation of the C-terminal domain of TopoII.
Article
Plant Sciences
Jihee Hong, Dasom Gwon, Chang-Young Jang
Summary: Ginsenoside Rg1 reduces cancer cell proliferation by inhibiting Haspin kinase activity and H3T3ph, leading to decreased levels of Aurora B at the centromere.
JOURNAL OF GINSENG RESEARCH
(2022)
Article
Cell Biology
Ines Berenguer, Pablo Lopez-Jimenez, Irene Mena, Alberto Viera, Jesus Page, Jose Gonzalez-Martinez, Carolina Maestre, Marcos Malumbres, Jose A. Suja, Rocio Gomez
Summary: The haspin-H3T3ph-chromosomal passenger complex (CPC) pathway is crucial for the regulation of centromeres during mammalian male meiosis, controlling chromosome segregation and spindle attachment. This study provides new insights into the functions of haspin kinase and the H3T3ph histone mark in meiotic centromere regulation.
JOURNAL OF CELL SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Marco A. Andonegui-Elguera, Rodrigo E. Caceres-Gutierrez, Alejandro Lopez-Saavedra, Fernanda Cisneros-Soberanis, Montserrat Justo-Garrido, Jose Diaz-Chavez, Luis A. Herrera
Summary: During mitosis, chromatin undergoes various activities regulated by histone post-translational modifications (HPTMs). Specific HPTMs play roles in chromosome condensation, centromere formation, and the assembly of regulatory protein complexes. Additionally, environmental factors can modify HPTMs, affecting chromosome segregation and genome stability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Joao Barbosa, Claudio E. Sunkel, Carlos Conde
Summary: This review provides an overview of the molecular strategies that monitor and fine-tune KT-MT attachment formation during mitosis, to ensure accurate segregation of sister chromatids. This process is crucial for safe mitotic progression.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Ana Boavida, Diana Santos, Mohammad Mahtab, Francesca M. Pisani
Summary: In eukaryotic cells, a tight connection exists between DNA replication and sister chromatid cohesion via cohesin. Cohesin is loaded onto chromatin in telophase/G1 and converted into a cohesive state during S phase. Down-regulation of DNA replication factors can lead to cohesion defects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Qi Yi, Qinfu Chen, Cai Liang, Haiyan Yan, Zhenlei Zhang, Xingfeng Xiang, Miao Zhang, Feifei Qi, Linli Zhou, Fangwei Wang
Article
Cell Biology
Feifei Qi, Qinfu Chen, Hongxia Chen, Haiyan Yan, Binbin Chen, Xingfeng Xiang, Cai Liang, Qi Yi, Miao Zhang, Hankun Cheng, Zhenlei Zhang, Jun Huang, Fangwei Wang
Article
Multidisciplinary Sciences
Haiyan Yan, Xingfeng Xiang, Qinfu Chen, Xuan Pan, Hankun Cheng, Fangwei Wang
SCIENTIFIC REPORTS
(2018)
Article
Biochemistry & Molecular Biology
Cai Liang, Zhenlei Zhang, Qinfu Chen, Haiyan Yan, Miao Zhang, Xingfeng Xiang, Qi Yi, Xuan Pan, Hankun Cheng, Fangwei Wang
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Cell Biology
Cai Liang, Zhenlei Zhang, Qinfu Chen, Haiyan Yan, Miao Zhang, Linli Zhou, Junfen Xu, Weiguo Lu, Fangwei Wang
JOURNAL OF CELL BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Cai Liang, Qinfu Chen, Qi Yi, Miao Zhang, Haiyan Yan, Bo Zhang, Linli Zhou, Zhenlei Zhang, Feifei Qi, Sheng Ye, Fangwei Wang
Article
Biochemistry & Molecular Biology
Linli Zhou, Cai Liang, Qinfu Chen, Zhenlei Zhang, Bo Zhang, Haiyan Yan, Feifei Qi, Miao Zhang, Qi Yi, Youchen Guan, Xingfeng Xiang, Xiaoqing Zhang, Sheng Ye, Fangwei Wang