Article
Biochemistry & Molecular Biology
Sanghoon Lee, Hyun-Woong Cho, Boram Kim, Jae Kwan Lee, Tak Kim
Summary: The study showed that slow-freezing and transplantation with Z-VAD is an effective method to preserve primordial follicle counts, decrease double-strand DNA breaks, and increase angiogenesis in a mouse model. Further molecular and clinical studies are needed to confirm these results.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Endocrinology & Metabolism
Marcos Eiji Shiroma, Luciana Lamarao Damous, Fernanda Pereira Cotrim, Cristiane Lima Roa, Jose Cipolla-Neto, Russel Joseph Reiter, Edmund Chada Baracat, Jose Maria Soares Jr
Summary: Melatonin pretreatment improves the quality of rat ovarian grafts by promoting the restart of the estrous cycle, increasing the expression of various key proteins in the ovaries, and reducing apoptosis levels. Further studies on reproductive function enhancement are warranted based on these findings.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2021)
Review
Obstetrics & Gynecology
Marie-Madeleine Dolmans, Michael von Wolff, Catherine Poirot, Cesar Diaz-Garcia, Luciana Cacciottola, Nicolas Boissel, Jana Liebenthron, Antonio Pellicer, Jacques Donnez, Claus Yding Andersen
Summary: The feasibility of freezing and thawing ovarian tissue is well-documented, but ovarian tissue transplantation is progressing at a slower pace with limited clinical experience. Results from five European centers show a low risk of relapse with ovarian tissue reimplantation and successful recovery of endocrine function in most patients, although in vitro fertilization efficacy needs improvement. Chemotherapy before ovarian tissue cryopreservation does not significantly affect success rates. Further research is needed to optimize the approach for ovarian tissue transplantation.
FERTILITY AND STERILITY
(2021)
Review
Endocrinology & Metabolism
Ashley A. Diaz, Hana Kubo, Nicole Handa, Maria Hanna, Monica M. Laronda
Summary: Ovarian tissue cryopreservation is the only option for preserving fertility in prepubescent girls and patients who cannot undergo ovarian stimulation. Despite the lack of standardized processing methods, ovarian tissue transplantation can restore ovarian hormone activity regardless of processing size.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Endocrinology & Metabolism
Raffaella Fabbri, Rossella Vicenti, Valentina Magnani, Roberto Paradisi, Mario Lima, Lucia De Meis, Stefania Rossi, Diego Raimondo, Paolo Casadio, Stefano Venturoli, Michela Maffi, Renato Seracchioli
Summary: This study reports the 20-year experience of the Bologna clinical center in ovarian tissue cryopreservation and transplantation. The results show that ovarian tissue cryopreservation is an effective technique for preserving ovarian function in pediatric and adult patients, and it can help restore menstruation and achieve pregnancy.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Jeong Min Kim, Seongmin Kim, Sanghoon Lee
Summary: Although cancer treatments can impact fertility in women, preserving fertility is crucial for quality of life. Fertility experts should propose personalized preservation methods, with ovarian tissue cryopreservation and transplantation emerging as a potential solution. Using stem cells in this process can improve fertilization techniques.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Endocrinology & Metabolism
Jing Chen, Yan Han, Wenjie Shi, Xiaohong Yan, Yingying Shi, Ye Yang, Hong Gao, Youzhu Li
Summary: Cryopreservation of ovarian tissue is an alternative method for fertility preservation and hormone replacement. It is suitable for cancer patients, patients in urgent need of cancer treatment, and women with premature ovarian failure or menopause, providing physiological doses of hormones while avoiding risks associated with oral hormone replacement.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Obstetrics & Gynecology
Stine G. Kristensen, Hanna O. Olesen, Mette C. Zeuthen, Susanne E. Pors, Claus Y. Andersen, Linn S. Mamsen
Summary: The study found that host revascularization of transplanted human ovarian cortex occurred with equal efficiency from both sides, suggesting that techniques ensuring revascularization from both sides could facilitate faster graft revascularization. Additionally, a significantly lower vessel density was observed in the center of the human ovarian grafts compared to the cortical and medullary sides. Microvessels comprised the majority of all vessels in the three defined regions of interest.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Obstetrics & Gynecology
Stine Gry Kristensen, Yu Wakimoto, Lotte Berdiin Colmorn, Margit Dueholm, Susanne Elisabeth Pors, Kirsten Tryde Macklon, Linn Salto Mamsen, Dmitry Nikiforov, Jesus Cadenas, Vinnie Hornshoj Greve, Anne-Mette Bay Bjorn, Mikkel Rosendahl, Anette Tonnes Pedersen, Anders Nyboe Andersen, Jens Fedder, Erik Ernst, Claus Yding Andersen
Summary: This study evaluated the use of cryopreserved ovarian tissue in the Danish fertility preservation cohort, revealing that OTT rates depended on diagnosis, age at OTC, and follow-up time. Longer follow-up is needed, especially for younger girls.
FERTILITY AND STERILITY
(2021)
Article
Pathology
Yongqi Wu, Jieqiong Huang, Hui Chen, Huan Tao, Yunbiao He, Guang Yang, Qingbing Zha, Gendie E. Lash, Ping Li
Summary: Breast cancer can lead to ovarian tissue dysfunction, affecting the effectiveness of ovarian cryopreservation and requiring additional interventions. The study shows that breast cancer causes the loss of primordial and growing follicles, as well as a decrease in follicle numbers. The gene expression analysis of tumor-bearing mice reveals disturbed steroidogenesis regulatory network and the relationship between phosphatidylinositol 3-kinase signaling pathway and mitochondrial dysfunction in oocytes and granulosa cells. Increase in reactive oxygen species is found in serum and ovarian tissues of tumor-bearing mice. Additionally, supplementation with vitamin C promotes follicular quiescence and repairs tumor-induced follicle loss through inactivation of the phosphatidylinositol 3-kinase-Akt-mammalian target of rapamycin pathway, suggesting the potential of antioxidants as a fertility therapy for achieving more healthy follicles ready for ovarian cryopreservation.
AMERICAN JOURNAL OF PATHOLOGY
(2023)
Article
Reproductive Biology
Lingbo Meng, Yodo Sugishita, Sandy Nishimura, Atsushi Uekawa, Yuki Suzuki-Takahashi, Nao Suzuki
Summary: This study aimed to investigate the optimal culture time and viability of postwarmed ovarian tissues before transplantation. The findings suggest that a culture time of 1-2 hours is optimal for vitrified-warmed tissues.
BIOLOGY OF REPRODUCTION
(2022)
Article
Oncology
Lotte B. Colmorn, Anette T. Pedersen, Elisabeth C. Larsen, Alexandra S. Hansen, Mikkel Rosendahl, Claus Yding Andersen, Stine G. Kristensen, Kirsten T. Macklon
Summary: This study reports the reproductive outcome and hormonal recovery following ovarian tissue transplantation and evaluates possible predictors of the chance of pregnancy. Ovarian tissue cryopreservation is a safe and promising method to restore fertility. The live birth rate after ovarian tissue transplantation is encouraging, and the chance of pregnancy is correlated to the ovarian reserve at the time of cryopreservation.
Article
Obstetrics & Gynecology
Marie-Madeleine Dolmans, Jacques Donnez
Summary: Approximately 10% of cancers occur in women under the age of 45, with chemotherapy, radiotherapy, and bone marrow transplantation curing more than 90% of cases but potentially leading to premature ovarian insufficiency. Fertility preservation may also be necessary for benign diseases or social reasons. Oocyte vitrification and ovarian tissue cryopreservation are two main strategies for preserving fertility in female cancer patients.
BEST PRACTICE & RESEARCH CLINICAL OBSTETRICS & GYNAECOLOGY
(2021)
Article
Endocrinology & Metabolism
Xiangyan Ruan, Jiaojiao Cheng, Juan Du, Fengyu Jin, Muqing Gu, Yanglu Li, Rui Ju, Yurui Wu, Huanmin Wang, Wei Yang, Haiyan Cheng, Long Li, Wenpei Bai, Weimin Kong, Xin Yang, Shulan Lv, Yuejiao Wang, Yu Yang, Xin Xu, Lingling Jiang, Yanqiu Li, Alfred O. Mueck
Summary: This study investigates the effectiveness and feasibility of ovarian tissue cryopreservation (OTC) in children facing gonadotoxicity treatment. The results suggest that OTC is a safe and effective method for preserving the fertility of prepubertal girls, and chemotherapy does not affect the effectiveness of OTC.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Obstetrics & Gynecology
Kutluk Oktay, Loris Marin, Giuliano Bedoschi, Fernanda Pacheco, Yodo Sugishita, Tai Kawahara, Enes Taylan, Carlo Acosta, Heejung Bang
Summary: Robot-assisted autologous cryopreserved ovarian tissue transplantation (RA-ACOTT) resulted in extended graft longevity, with ovarian functions restored in all cases.
FERTILITY AND STERILITY
(2022)