Article
Biochemistry & Molecular Biology
Aida Kamalian, Sara G. Ho, Megha Patel, Alexandria Lewis, Arnold Bakker, Marilyn Albert, Richard J. O'Brien, Abhay Moghekar, Michael W. Lutz
Summary: This study measured 3072 proteins in the CSF samples of AD-biomarker positive MCI patients and controls, and found an upregulation of neuroinflammatory pathways, particularly chemotactic and granulocyte recruitment pathways, in the CSF of early AD patients.
Article
Biotechnology & Applied Microbiology
Amanda Cano, Ester Esteban-de-Antonio, Mireia Bernuz, Raquel Puerta, Pablo Garcia-Gonzalez, Itziar de Rojas, Claudia Olive, Alba Perez-Cordon, Laura Montrreal, Raul Nunez-Llaves, Oscar Sotolongo-Grau, Emilio Alarcon-Martin, Sergi Valero, Montserrat Alegret, Elvira Martin, Pamela V. Martino-Adami, Miren Ettcheto, Antonio Camins, Assumpta Vivas, Marta Gomez-Chiari, Miguel angel Tejero, Adelina Orellana, Lluis Tarraga, Marta Marquie, Alfredo Ramirez, Merce Marti, Maria Isabel Pividori, Merce Boada, Agustin Ruiz
Summary: Alzheimer's disease (AD) is commonly preceded by a prodromal phase characterized by high levels of A beta and p-tau-mediated neuronal injury and mild cognitive impairment (MCI). Differential diagnosis of MCI causes in these prodromal stages is a challenge for clinicians. Blood biomarkers, particularly plasma extracellular vesicles (pEVs), are emerging as potential tools for early AD detection.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Clinical Neurology
Aurore Delvenne, Johan Gobom, Betty Tijms, Isabelle Bos, Lianne M. Reus, Valerija Dobricic, Mara Ten Kate, Frans Verhey, Inez Ramakers, Philip Scheltens, Charlotte E. Teunissen, Rik Vandenberghe, Jolien Schaeverbeke, Silvy Gabel, Julius Popp, Gwendoline Peyratout, Pablo Martinez-Lage, Mikel Tainta, Magda Tsolaki, Yvonne Freund-Levi, Simon Lovestone, Johannes Streffer, Frederik Barkhof, Lars Bertram, Kaj Blennow, Henrik Zetterberg, Pieter Jelle Visser, Stephanie J. B. Vos
Summary: The pathophysiology of MCI-SNAP is distinct from that of MCI-AD, highlighting the need for different treatment approaches.
ALZHEIMERS & DEMENTIA
(2023)
Article
Immunology
Rong Yin, Linpeng Yang, Ying Hao, Zhiqi Yang, Tao Lu, Wanjun Jin, Meiling Dan, Liang Peng, Yingjie Zhang, Yaxuan Wei, Rong Li, Huiping Ma, Yuanyuan Shi, Pengcheng Fan
Summary: This study analyzed the clinical and proteomic data of patients with Japanese encephalitis (JE) and identified two subtypes of JE with significantly different prognoses. The JE2 subtype was associated with a higher risk of cognitive impairment. The findings also provided potential target proteins for the treatment of cognitive impairments caused by JE.
JOURNAL OF NEUROINFLAMMATION
(2022)
Article
Neurosciences
Catarina Bernardes, Marisa Lima, Diana Duro, Anuschka Silva-Spinola, Joao Duraes, Miguel Tabuas-Pereira, Ines Baldeiras, Sandra Freitas, Isabel Santana
Summary: This study confirms the role of cerebrospinal fluid Alzheimer's disease biomarkers in predicting conversion from mild cognitive impairment to dementia and suggests the utility of Montreal Cognitive Assessment in predicting conversion in highly educated subjects, supporting its use in the evaluation of MCI patients.
JOURNAL OF ALZHEIMERS DISEASE
(2023)
Article
Neurosciences
Akram A. Hosseini, Thomas Brown, Luca Mannino, Bruno Gran, Kehinde Junaid, Elizabeta B. Mukaetova-Ladinska
Summary: This study conducted diagnostic lumbar puncture in patients with early onset Alzheimer's or related dementias and evaluated the measurements of amyloid-beta 42 (A beta(42)), total tau, and Thr181-phosphorylated tau (p-tau) in the cerebrospinal fluid (CSF). The results showed that low levels of CSF A beta(42) appear to be more sensitive than total and p-tau measures in differentiating AD MCI from other forms of dementia.
JOURNAL OF ALZHEIMERS DISEASE
(2022)
Article
Neurosciences
Kate J. Russin, K. Sreekumaran Nair, Thomas J. Montine, Laura D. Baker, Suzanne Craft
Summary: This study aimed to investigate the impact of diets with different nutrient compositions on cerebrospinal fluid amino acid levels, finding that a high saturated fat/glycemic index diet can lead to changes in amino acid levels in individuals with mild cognitive impairment. The response to diet intervention varies according to cognitive status.
JOURNAL OF ALZHEIMERS DISEASE
(2021)
Article
Multidisciplinary Sciences
Anna Daily, Prashanth Ravishankar, Steve Harms, V. Suzanne Klimberg
Summary: This study identified protein biomarkers with altered expression levels in tear samples from women diagnosed with breast cancer. Through LC-MS/MS analysis, 14 potential biomarkers were identified and validated by ELISA. The results showed significantly higher expression of S100A8 and S100A9 in breast cancer patients, while Galectin-3 binding protein had increased expression in the control group. These findings support the use of tear proteins in detecting systemic diseases such as breast cancer.
Article
Biochemistry & Molecular Biology
Sungtaek Oh, Yura Jang, Chan Hyun Na
Summary: This study used discovery-based approaches and quantitative comparative analyses to identify differential proteins in the cerebrospinal fluid (CSF) of patients with ALS. They found 15 proteins that showed significant differences between ALS and the control group, providing a foundation for biomarker research in ALS.
Article
Cell Biology
Yael Hirschberg, Natalia Valle-Tamayo, Oriol Dols-Icardo, Sebastiaan Engelborghs, Bart Buelens, Roosmarijn E. Vandenbroucke, Yannick Vermeiren, Kurt Boonen, Inge Mertens
Summary: Dementia is a leading cause of death worldwide, and this study focused on analyzing the proteome of cerebrospinal fluid (CSF) and CSF-derived extracellular vesicles (EVs) from patients with Alzheimer's disease, dementia with Lewy bodies, and Parkinson's disease dementia. The analysis identified differentially expressed proteins that can be helpful for differential diagnosis and offer new avenues for research into disease-specific pharmacological therapeutics.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Review
Neurosciences
Elena Sagues-Sese, Jose Rioja, Francisco J. Garzon-Maldonado, Manuel Narvaez, Juan A. Garcia-Arnes, Natalia Garcia-Casares
Summary: This systematic review provides an overview of the state-of-the-art knowledge about insulin-related cerebrospinal fluid (CSF) biomarkers in Alzheimer's disease (AD) and mild cognitive impairment (MCI). The results indicate decreased levels of CSF insulin and increased levels of insulin-related growth factors and their binding proteins.
JOURNAL OF ALZHEIMERS DISEASE
(2022)
Article
Geriatrics & Gerontology
Farida Dakterzada, Mariona Jove, Raquel Huerto, Anna Carnes, Joaquim Sol, Reinald Pamplona, Gerard Pinol-Ripoll
Summary: Genetic, metabolic, and clinical evidence shows that lipid dysregulation is associated with an increased risk of Alzheimer's disease (AD). This study investigated the association between cerebrospinal fluid (CSF) lipidome and the pathological hallmarks of AD, progression from mild cognitive impairment (MCI) to AD, and the rate of cognitive decline in MCI patients. The results suggest that CSF neutral lipids may serve as potential prognostic markers for AD.
Article
Neurosciences
Zahinoor Ismail, Rebeca Leon, Byron Creese, Clive Ballard, Philippe Robert, Eric E. Smith
Summary: This study investigated the association between mild behavioral impairment (MBI) in mild cognitive impairment (MCI) and Alzheimer's disease (AD), and found that adding MBI to MCI can better identify AD and improve dementia prognostication. Therefore, the addition of MBI is important for the detection of AD.
MOLECULAR NEURODEGENERATION
(2023)
Review
Biochemistry & Molecular Biology
Yunxing Ma, Julia Brettschneider, Joanna F. Collingwood
Summary: Levels of amyloid-beta and tau in human cerebrospinal fluid were evaluated to predict the transition from Mild Cognitive Impairment to Alzheimer's disease. The results support the value of amyloid-beta and tau as markers of patient outcome.
Article
Geriatrics & Gerontology
Yuanyuan Lu
Summary: CSF GAP-43 levels are elevated in AD and may play a role in cognitive decline and neurodegeneration.
AGING CLINICAL AND EXPERIMENTAL RESEARCH
(2022)
Article
Hematology
Fredrik Granholm, Dan Bylund, Ganna Shevchenko, Sara B. Lind, Anders E. Henriksson
Summary: Acute pulmonary embolism is a common emergency condition with limited biomarkers for early diagnosis. This study utilized label-free quantitative proteomics to identify potential biomarkers for acute PE and monitor changes in protein levels over time.
CLINICAL AND APPLIED THROMBOSIS-HEMOSTASIS
(2022)
Article
Biochemical Research Methods
Henrik Carlsson, Aina Vaivade, Payam Emami Khoonsari, Joachim Burman, Kim Kultima
Summary: Untargeted lipidomics analysis using liquid chromatography high-resolution mass spectrometry was performed with polarity switching and separate polarities, showing that using separate polarities can reduce the coefficient of variation and detect slightly more endogenous features, with low overlap between positive and negative polarities, highlighting the importance of using both polarities for untargeted lipidomics analysis. The results also indicated that both methodologies provided highly similar biological results, confirming the applicability of polarity switching.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Geriatrics & Gerontology
Alex B. Addinsall, Nicola Cacciani, Anders Backeus, Yvette Hedstrom, Ganna Shevchenko, Jonas Bergquist, Lars Larsson
Summary: This study found that chronic supramaximal electrical stimulation (ES) can alleviate the symptoms of critical illness myopathy (CIM) and maintain muscle glucose homeostasis by increasing the AMPK-TBC1D4 signaling pathway, reducing muscle protein degradation.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2022)
Article
Medicine, Research & Experimental
Avinash Khandagale, Bertil Lindahl, Sara Bergstrom Lind, Ganna Shevchenko, Agneta Siegbahn, Christina Christersson
Summary: Endogenous EVs from myocardial infarction (MI) patients have cardioprotective potential against TNFα-induced cell death, and further research is needed to elucidate their role in MI.
CURRENT RESEARCH IN TRANSLATIONAL MEDICINE
(2022)
Article
Biochemical Research Methods
Verena E. Kutschera, Marcin Kierczak, Tom van der Valk, Johanna von Seth, Nicolas Dussex, Edana Lord, Marianne Dehasque, David W. G. Stanton, Payam Emami Khoonsari, Bjorn Nystedt, Love Dalen, David Diez-del-Molino
Summary: The study presents a flexible, scalable, and modular pipeline for comparing patterns of genomic erosion in samples from different time periods. The pipeline utilizes state-of-the-art bioinformatics tools to process whole-genome re-sequencing data from ancient/historical and modern samples, and produces comparable estimates of genomic erosion indices.
BMC BIOINFORMATICS
(2022)
Article
Neurosciences
Karl Sjolin, Kim Kultima, Anders Larsson, Eva Freyhult, Christina Zjukovskaja, Kanar Alkass, Joachim Burman
Summary: This study determined the tissue concentrations of GFAP, MBP, NFL, tau, and UCHL1 in different regions of the central nervous system using ELISA. The concentrations of these proteins varied substantially between different CNS regions. These findings can serve as a reference for interpreting the circulating levels of these biomarkers in relation to the extent and localization of CNS-damaging processes.
Article
Cardiac & Cardiovascular Systems
Karl Sjolin, Julia Aulin, Lars Wallentin, Niclas Eriksson, Claes Held, Kim Kultima, Jonas Oldgren, Joachim Burman
Summary: sNFL levels are higher in patients with atrial fibrillation (AF) compared to those without AF. Patients with ongoing AF rhythm have even higher sNFL levels compared to patients diagnosed with AF but not currently in AF rhythm.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2022)
Article
Medical Laboratory Technology
Niclas Rollborn, Jenny Jakobsson, Andrew Campbell, Gunnar Nordin, Mathias Karlsson, Anders Larsson, Kim Kultima
Summary: The study found significant deviations in FLC measurements among different methods, with lower deviation when only nephelometry was used. There were significant differences in coefficient of variation between the two main FLC assays, and generally good coefficient of determination between reagents and instrument platforms.
CLINICAL BIOCHEMISTRY
(2023)
Article
Clinical Neurology
Andreas Giannisis, Asma Al-Grety, Henrik Carlsson, Kalicharan Patra, Daniel Twohig, Sigrid Botne Sando, Camilla Lauridsen, Guro Berge, Goril Rolfseng Grontvedt, Geir Brathen, Linda R. White, Kim Kultima, Henrietta M. Nielsen
Summary: This study found important associations between low plasma apoE levels and increased risk of Alzheimer's disease (AD), as well as progression from mild cognitive impairment to a clinical AD diagnosis.
ALZHEIMERS RESEARCH & THERAPY
(2022)
Article
Clinical Neurology
Christina Zjukovskaja, Anders Larsson, Honar Cherif, Kim Kultima, Joachim Burman
Summary: This study found that in most patients, biomarkers of demyelination and axonal damage reached normal values within five years from treatment with aHSCT.
MULTIPLE SCLEROSIS AND RELATED DISORDERS
(2022)
Article
Genetics & Heredity
Maria Needhamsen, Payam Emami Khoonsari, Galina Yurevna Zheleznyakova, Eliane Piket, Michael Hagemann-Jensen, Yanan Han, Jannik Gierlich, Diana Ekman, Maja Jagodic
Summary: This study utilizes large-scale data processing and analysis to extract small RNA data from multiple sclerosis patients and neurological disease controls, and applies data integration methods to classify disease status. The findings demonstrate that small RNA data can be used to distinguish different disease stages and control groups, which is of great significance for early diagnosis and treatment.
FRONTIERS IN GENETICS
(2022)
Article
Neurosciences
Makoto Shimozawa, Helene Tigro, Henrik Biverstal, Ganna Shevchenko, Jonas Bergquist, Ruin Moaddel, Jan Johansson, Per Nilsson
Summary: Proteins must fold into three-dimensional structures to carry out their functions, and maintaining protein homeostasis is crucial for cellular activity and health. Molecular chaperones, such as BRICHOS domain, play key roles in proteostasis and can interfere with aggregation-prone proteins, including amyloid beta associated with Alzheimer's disease. In this study, the authors used a recombinant human Bri2 BRICHOS-mCherry fusion protein to identify potential binding partners. Through staining and co-immunoprecipitation experiments, they found that Bri2 BRICHOS interacts with cytoskeletal proteins, suggesting a possible interplay between extracellular chaperones and the intracellular space, including the cytoskeleton.
MOLECULAR AND CELLULAR NEUROSCIENCE
(2023)
Article
Multidisciplinary Sciences
Stephanie Herman, Staffan Arvidsson McShane, Christina Zjukovskaja, Payam Emami Khoonsari, Anders Svenningsson, Joachim Burman, Ola Spjuth, Kim Kultima
Summary: Currently, there is a need for biomarkers to assist in early diagnosis of progressive multiple sclerosis (PMS). Research has shown that a selection of cerebrospinal fluid metabolites can differentiate between PMS and its preceding phenotype. By using predictive methods, highly confident predictions can be made for patients who will develop PMS within three years. In a clinical trial, the methodology was applied to PMS patients receiving intrathecal treatment, and it was found that 68% of the patients decreased their similarity to the PMS phenotype after one year of treatment.
Article
Chemistry, Analytical
Henrik Carlsson, Akshai Parakkal Sreenivasan, Ida Erngren, Anders Larsson, Kim Kultima
Summary: We have developed a method for screening environmental contaminants in human plasma using LC-HRMS. We analyzed plasma samples from 100 blood donors and detected 19 target compounds, including PFASs and OH-PCBs. Some compounds showed positive associations with age and sex, and strong correlations were observed between certain PFAS compounds. In non-targeted analysis, unknown features correlating with PFASs were found, and several endogenous compounds were identified. This method allows for the detection of previously unknown associations between environmental contaminants and endogenous compounds, which is important for human health.
ENVIRONMENTAL SCIENCE-PROCESSES & IMPACTS
(2023)
Article
Clinical Neurology
Joachim Burman, Christina Zjukovskaja, Anders Svenningsson, Eva Freyhult, Anna Wiberg, Kim Kultima
Summary: This study explored the differences in cytokines between individuals with multiple sclerosis and healthy controls, and investigated how these cytokines were affected by autologous haematopoietic stem cell transplantation or intrathecal rituximab treatment. The results suggest a profound effect of autologous haematopoietic stem cell transplantation on the inflammatory milieu of the cerebrospinal fluid in multiple sclerosis.
BRAIN COMMUNICATIONS
(2022)