☆ 4.4 Article

A Ferredoxin Disulfide Reductase Delivers Electrons to the Methanosarcina barkeri Class III Ribonucleotide-Reductase

BIOCHEMISTRY (2015)

期刊

BIOCHEMISTRY

卷 54, 期 47, 页码 7019-7028

出版社

AMER CHEMICAL SOC

DOI: 10.1021/acs.biochem.5b01092

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Biochemistry & Molecular Biology

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ASTAR Singapore
Division of Chemical Sciences, Geosciences, and Biosciences, Office of Basic Energy Sciences, U.S. Department of Energy [DE-FG02-95ER20198 MOD16]
National Science Foundation [MCB 1122977]
National Institutes of Health [GM081393]

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摘要

Two subtypes of class III anaerobic ribonucleotide reductases (RNRs) studied so far couple the reduction of ribonucleotides to the oxidation of formate, or the oxidation of NADPH via thioredoxin and thioredoxin reductase. Certain methanogenic archaea contain a phylogenetically distinct third subtype of class III RNR, with distinct active-site residues. Here we report the cloning and recombinant expression of the Methanosarcina barkeri class III RNR and show that the electrons required for ribonucleotide reduction can be delivered by a [4Fe-4S] protein ferredoxin disulfide reductase, and a conserved thioredoxin-like protein NrdH present in the RNR operon. The diversity of class III RNRs reflects the diversity of electron carriers used in anaerobic metabolism.

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