期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 67, 期 1, 页码 372-378出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.8b06260
关键词
rapeseed; canola; bitter taste; taste dilution analysis; kaempferol 3-O-(2 '''-O-sinapoyl-beta-sophoroside)
资金
- Federal Ministry of Education and Research of Germany (BMBF) [031B0198D]
By means of activity-guided fractionation using taste dilution analysis, liquid chromatography-tandem mass spectrometry (LC-MS/MS), liquid chromatography-time-of-flight mass spectrometry, one-/two-dimensional nuclear magnetic resonance spectroscopy, LC-MS/MS quantitation, dose-over-threshold considerations, and sensory spiking experiments, kaempferol 3-O-(2'-O-sinapoyl-beta-sophoroside), exhibiting a bitter taste above the low threshold concentration of 3.4 mu mol/L, was found for the first time as the key molecule contributing to the unpleasant bitter taste of rapeseed (canola) protein isolates. This finding opens new avenues for a biorefinery approach targeting an off-taste removal.
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