Review
Biochemistry & Molecular Biology
Luisa B. B. Maia
Summary: Enzymes of the xanthine oxidase family containing molybdenum are known for catalyzing oxygen atom transfer reactions, but their ability to abstract an oxygen atom from the substrate molecule is not widely recognized. The discovery of mammalian xanthine oxidase's capability to catalyze the oxygen atom abstraction of nitrite to form nitric oxide (NO) was met with surprise and skepticism from the molybdenum community. Through extensive studies on enzymes of various families, it is now widely recognized that molybdoenzymes have the ability to form NO from nitrite.
Article
Agriculture, Dairy & Animal Science
Karlygash Aubakirova, Mereke Satkanov, Maral Kulataeva, Gulmira Assylbekova, Aigul Kambarbekova, Zerekbai Alikulov
Summary: Nitric oxide (NO) plays essential physiological functions in terrestrial animals, including mammals and ectothermic vertebrates such as fish. However, there is limited knowledge about the activity and properties of molybdoenzymes in fish organs, which can convert nitrate and nitrite into NO. This study aims to detect the activity of molybdoenzymes in fish liver and their ability to reduce nitrate and nitrite to nitrogen monoxide.
CZECH JOURNAL OF ANIMAL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Joaquin Ortiz de Zevallos, Mary N. Woessner, Eric E. Kelley
Summary: Recent studies have shown that skeletal muscle is a storage site for nitrate and nitrite, which can help maintain nitric oxide levels. The enzyme xanthine oxidoreductase (XOR) has been proposed as a key player in reducing these compounds, but its role in skeletal muscle and the factors that determine its contribution to nitric oxide production are not well understood.
NITRIC OXIDE-BIOLOGY AND CHEMISTRY
(2022)
Article
Microbiology
Anne-Soisig Steunou, Marion Babot, Anne Durand, Marie-Line Bourbon, Sylviane Liotenberg, Guylaine Miotello, Jean Armengaud, Soufian Ouchane
Summary: The high toxicity of Cu, Cd, Pb, As, and other metals arises from their ability to cross membranes and target metalloenzymes in the cytoplasm. Identifying these targets provides insights into the toxicity mechanisms. The vulnerability of metalloenzymes arises from the accessibility of their cofactors to ions.
MICROBIOLOGY SPECTRUM
(2023)
Review
Biochemistry & Molecular Biology
Mai Sekine, Ken Okamoto, Kimiyoshi Ichida
Summary: Xanthine oxidoreductase (XOR) is a key enzyme in purine metabolism, with complex structure and function well studied. XOR inhibitors are used to treat hyperuricemia and gout, with potential effects beyond uric acid reduction. Xanthinuria serves as a good model for studying XOR mutations and activity, shedding light on the biological role and reaction mechanism of XOR.
Article
Pharmacology & Pharmacy
Zoe Coombes, Katie Plant, Cristina Freire, Abdul W. Basit, Philip Butler, R. Steven Conlan, Deyarina Gonzalez
Summary: Progesterone has low and variable bioavailability after oral administration, with significant contributions to its clearance from both the liver and intestine. Metabolism of progesterone in the liver is rapid, while the intestine also plays a role in progesterone degradation. Understanding the kinetics of both intestinal and liver metabolism is crucial for developing effective progesterone oral formulations in the future.
Article
Biochemistry & Molecular Biology
Gary Cecchini
Summary: High levels of H2S produced by gut microbiota can inhibit the utilization of oxygen by blocking mitochondrial complex IV. Cells respond to this inhibition by utilizing the mitochondrial sulfide oxidation pathway and reverse electron transport. The reversal activity of mitochondrial complex II during hypoxia and cellular stress plays a crucial role in oxidizing H2S.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Elisabetta Marini, Marta Giorgis, Marta Leporati, Barbara Rolando, Konstantin Chegaev, Loretta Lazzarato, Massimo Bertinaria, Marco Vincenti, Antonella Di Stilo
Summary: This study investigated the oxidative stress hypothesis of nitrate tolerance by characterizing newly synthesized organic nitrates with and without antioxidant properties. The results showed that the ability of compounds to interact with the ALDH-2 enzyme was related to their vasodilation and tolerance profiles. Furthermore, organic nitrates with antioxidant properties did not induce tolerance, supporting the involvement of reactive oxygen species (ROS) in inactivating ALDH-2.
Article
Peripheral Vascular Disease
Masato Furuhashi, Yukimura Higashiura, Masayuki Koyama, Marenao Tanaka, Takayo Murase, Takashi Nakamura, Seigo Akari, Akiko Sakai, Kazuma Mori, Hirofumi Ohnishi, Shigeyuki Saitoh, Kazuaki Shimamoto, Tetsuji Miura
Summary: The study found that plasma XOR activity is independently associated with hypertension in nondiabetic individuals who are not taking any medications. The activity is positively correlated with mean arterial pressure and significantly higher in the hypertension group compared to the non-hypertension group.
HYPERTENSION RESEARCH
(2021)
Article
Plant Sciences
Aprajita Kumari, Vemula Chandra Kaladhar, Nidhi Yadav, Pooja Singh, Kishorekumar Reddy, Kapuganti Jagadis Gupta
Summary: The location of NO production in mitochondrial cytochrome c oxidase and its role in mitochondrial biogenesis were investigated in Arabidopsis seedlings under osmotic stress and recovery. Osmotic stress reduced growth and mitochondrial number while increasing NO production. Nitrite application stimulated NO production and mitochondrial number in a NO-deficient mutant. COX6b(-)3 and COA6-L subunits were induced during osmotic stress and mutants impaired in these subunits showed reduced NO production and mitochondrial biogenesis. The COX6b-3 and COA6-L subunits interacted with a VQ27 motif-containing protein in the presence of NO, and a vq27 mutant displayed impaired mitochondrial biogenesis.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Biophysics
Tiago Monteiro, Miguel Moreira, Sara B. R. Gaspar, Maria Gabriela Almeida
Summary: This study proposes a new mono-enzymatic oxygen scavenging system using multicopper oxidase as a single biocatalytic oxygen reducer. This system shows quick and effective reduction of oxygen and can be applied in voltammetric biosensors without the need for a second enzyme to handle oxygen reactive intermediaries.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Pharmacology & Pharmacy
Nikhilesh V. Dhuria, Bianka Haro, Amit Kapadia, Khadjia A. Lobo, Bernice Matusow, Mary A. Schleiff, Christina Tantoy, Jasleen K. Sodhi
Summary: This paper comprehensively summarizes recent advancements in the prediction of drug metabolism and in vitro to in vitro extrapolation of clearance for substrates of non-CYP drug metabolizing enzymes, highlighting the challenges in accurately predicting non-CYP-mediated clearance.
DRUG METABOLISM REVIEWS
(2021)
Article
Pharmacology & Pharmacy
Brijesh Patel, Hetal Thakkar
Summary: Febuxostat is a widely prescribed drug for the treatment of gout worldwide. However, it has limitations such as gastrointestinal disturbances and low oral bioavailability. To overcome these limitations, transdermal drug delivery systems of Febuxostat were developed to improve patient compliance and bioavailability.
Article
Biochemistry & Molecular Biology
Sara E. Lewis, Courtney B. Rosencrance, Evan De Vallance, Andrew Giromini, Xena M. Williams, Joo-Yeun Oh, Heidi Schmidt, Adam C. Straub, Paul D. Chantler, Rakesh P. Patel, Eric E. Kelley
Summary: Research indicates that XOR does not contribute to nitrite reduction in human and rodent erythrocytes, suggesting a need to validate immuno-detectable XOR by demonstrating enzymatic activity.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
G. Massimo, R. S. Khambata, T. Chapman, K. Birchall, C. Raimondi, A. Shabbir, Nicki Dyson, K. S. Rathod, C. Borghi, A. Ahluwalia
Summary: XDH mutations have varying impacts on the biochemical activity of the enzyme, leading to potential harmful or protective influences on health. Targeting enzyme activity to enhance the nitrite-reductase profile may provide novel therapeutic options, especially in cardiovascular disease.
Article
Biochemistry & Molecular Biology
Cristina M. Cordas, Giang-Son Nguyen, Gabriel N. Valerio, Malene Jonsson, Katharina Sollner, Ingvild H. Aune, Alexander Wentzel, Jose J. G. Moura
Summary: A new dye-decolorizing peroxidase (DyP) with a unique EXXDG motif was discovered, showing the ability to oxidize various organic compounds under acidic and low temperature conditions. Kinetic and thermodynamic parameters of the catalytic redox center were determined through electrochemical assays.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Nadia Ribeiro, Ipek Bulut, Vivien Posa, Baris Sergi, Giuseppe Sciortino, Joao Costa Pessoa, Luisa B. Maia, Valeria Ugone, Eugenio Garribba, Eva A. Enyedy, Ceyda Acilan, Isabel Correia
Summary: We reported the synthesis and characterization of a family of benzohydrazones and their oxidovanadium(IV) complexes. The complexes showed high antiproliferative activity in cancer cells, possibly through oxidative stress-induced DNA damage and apoptosis.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Article
Chemistry, Physical
Raquel Viveiros, Luisa B. Maia, Marta C. Corvo, Vasco D. B. Bonifacio, William Heggie, Teresa Casimiro
Summary: A novel enzyme-inspired MIP catalytic system was designed for fast oxidation of cholesterol derivatives and easy catalyst regeneration. By synthesizing a template-monomer complex and crosslinked polymerization in scCO2, a TEMPO-MIP catalyst with high selective oxidation capacity was obtained, enabling the rapid oxidation of various substrates.
CATALYSIS COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Cristina M. Cordas, Gabriel N. Valerio, Anton Stepnov, Eirik Kommedal, Asmund R. Kjendseth, Zarah Forsberg, Vincent G. H. Eijsink, Jose J. G. Moura
Summary: Research on enzymes for lignocellulose biomass degradation has been increasing in recent years, especially the lytic polysaccharide monooxygenases (LPMOs) that can efficiently break down crystalline cellulose. The catalytic copper site in LPMOs plays a crucial role in their functionality and reactivity. In this study, electrochemical analysis was conducted to investigate the redox properties of an LPMO, and the effects of mutations on the copper site were also examined.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
A. Sofia Santos, Rita D. Ferro, Nuno Viduedo, Luisa B. Maia, Artur M. S. Silva, M. Manuel B. Marques
Summary: In this study, a simple method for synthesizing bis(3-indolyl)methanes (BIMs) derivatives in yields of up to 77% was developed by coupling indoles and benzyl alcohols using tBuOK as a catalyst.
Article
Biochemistry & Molecular Biology
Pedro M. S. Braganca, Marta S. P. Carepo, Sofia R. Pauleta, Tyler B. J. Pinter, Maddalena Elia, Cristina M. Cordas, Isabel Moura, Vincent L. Pecoraro, Jose J. G. Moura
Summary: The rational design and functionalization of peptides scaffolds with metal binding sites is investigated in this study. The incorporation of molybdenum into the de novo designed alpha 3DIV-L21C framework enhances its thermostability and stability despite minimal changes in secondary structure. The oxidation state of the Mo center in Mo-alpha 3DIV-L21C is proposed to be mainly Mo(VI) based on electrochemical, EPR, and UV-visible data.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Jose J. G. Moura
Summary: This manuscript presents a story that dates back almost 40 years. The author takes a different and challenging approach in reporting the research with the aim to reach a wide-ranging audience. The study is not presented in chronological order, but rather as a collection of topics developed over many years. The author hopes to give proper credit to their collaborators.
Review
Biochemistry & Molecular Biology
Luisa B. B. Maia
Summary: Enzymes of the xanthine oxidase family containing molybdenum are known for catalyzing oxygen atom transfer reactions, but their ability to abstract an oxygen atom from the substrate molecule is not widely recognized. The discovery of mammalian xanthine oxidase's capability to catalyze the oxygen atom abstraction of nitrite to form nitric oxide (NO) was met with surprise and skepticism from the molybdenum community. Through extensive studies on enzymes of various families, it is now widely recognized that molybdoenzymes have the ability to form NO from nitrite.
Article
Chemistry, Inorganic & Nuclear
Sara Realista, Paulo J. Costa, Luisa B. Maia, Maria Jose Calhorda, Paulo N. Martinho
Summary: Cyclic voltammetry and bulk electrolysis experiments showed that [Ni(ii)(salphen)] [1], [Ni(ii)(Bu-t-salphen)] [2], and a binuclear Ni(ii) compound combining salphen and Bu-t-salphen [3] can react with CO2 to generate a stable metal-carbonyl species under anaerobic conditions. When exposed to air, a stoichiometric amount of CO is released and protonation regenerates the initial complex. Through various spectroscopy studies and DFT calculations, it is proposed that the reduction of [Ni(i)(salphen)](-), (2)[1](-), leads to the formation of a CO2 complex, (2)[1(CO2)](-), which is further reduced to (3)[1(CO2)](2-). After protonation, the coordinated CO2 is reduced to CO and released, regenerating (1)[1]. The release of CO competes with CO2 in the second cycle, deactivating the system.
INORGANIC CHEMISTRY FRONTIERS
(2023)
Proceedings Paper
Computer Science, Artificial Intelligence
Cristina M. Cordas, M. Joao Nunes, Gabriel N. Valerio, Alejandro Samhan-Arias, Ludwig Krippahl, Jose J. G. Moura, Gilda Santos, Joao P. Sousa, Carlos Rouco
Summary: The study of human emotions through biomarkers is of high importance to society. Imbalanced physiological parameters can induce stress, which is related to diseases and discordant social behavior. Researchers are using non-invasive methodologies to detect stress biomarkers and are able to monitor them in real-time. Military volunteers are involved in proving the concept of this new medical methodology.
DEVELOPMENTS AND ADVANCES IN DEFENSE AND SECURITY, MICRADS 2021
(2022)
Article
Chemistry, Multidisciplinary
Stephanie Almada, Luisa B. Maia, Joao C. Waerenborgh, Bruno J. C. Vieira, Nuno P. Mira, Elisabete R. Silva, Fatima Cerqueira, Eugenia Pinto, Luis G. Alves
Summary: This article reports the synthesis and characterization of two new cyclam-based complexes. The experimental results indicate that these complexes exhibit high antifungal activity.
NEW JOURNAL OF CHEMISTRY
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
G. Valerio, A. K. Rohr, J. J. G. Moura, C. Cordas
Meeting Abstract
Biochemistry & Molecular Biology
J. Moura
Meeting Abstract
Biochemistry & Molecular Biology
C. M. Cordas, G. N. Valerio, A. K. Rohr, V. G. H. Eijsink, J. J. G. Moura
Meeting Abstract
Biochemistry & Molecular Biology
R. S. Duarte, C. M. Cordas, G. Nguyenb, G. N. Valerio, M. Jonsson, K. Sollner, I. H. Aune, A. Wentzel, J. J. G. Moura