期刊
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
卷 69, 期 1, 页码 159-164出版社
MICROBIOLOGY SOC
DOI: 10.1099/ijsem.0.003122
关键词
Nocardia aurea sp. nov.; karst cave; polyphasic taxonomy
类别
资金
- National Key R&D Program of China [2017YFD0200503]
- Natural Science Foundation of China [31600015]
- Natural Science Foundation of Guangdong Province, China [2016A030312003]
- National Fundamental Fund Project Subsidy Funds of Personnel Training of China [J1310025]
- Guangdong Province Higher Vocational Colleges and Schools Pearl River Scholar Funded Scheme
A novel actinobacterium, designated strain SYSU K10002(T), was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-western China. The taxonomic position of the strain was investigated using a polyphasic approach. Cells of the strain were aerobic and Gram-stain-positive. On the basis of 16S rRNA gene sequence similarities and phylogenetic analysis, strain SYSU K10002(T) was most closely related to the type strains of Nocardiaaltamirensis NBRC 108246(T) (99.0 % sequence similarity) and Nocardiatenerifensis NBRC 101015(T) (98.8 %) and is therefore considered to represent a member of the genus Nocardia. DNA-DNA hybridization values between strain SYSU K10002(T) and the closely related type strains of the genus Nocardia were less than 70 %. In addition, meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. The whole-cell sugars were arabinose, ribose and galactose. The major isoprenoid quinone was MK-8(H-4,omega-cycl), while the major fatty acids (>10 %) were C-16:0, C-18:1 omega 9C and C-18:0 10-methyl. The polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and an unidentified glycolipid. Mycolic acids were present. The genomic DNA G+C content of strain SYSU K10002(T) was 67.4 mol%. On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10002(T) represents a novel species of the genus Nocardia, for which the name Nocardia aurea sp. nov. is proposed. The type strain is SYSU K10002(T) (=KCTC 39849(T) =DSM 103986(T)).
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