Article
Oncology
Jolene Chisholm, Sandor Haas-Neill, Peter Margetts, Khalid Al-Nedawi
Summary: Prostate cancer (PC) is a major cause of death in men, and current biomarker tests have limitations in accurately diagnosing the disease. This study examined the protein, mRNA, and miRNA content of extracellular vesicles (EVs) collected from PC patients and healthy subjects. The analysis revealed distinct groups of proteins, mRNAs, and miRNAs that differed between healthy subjects and PC patients, as well as between different tumor types. These findings suggest potential molecular markers for diagnosing PC.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Giulia Furesi, Antonio Miguel de Jesus Domingues, Dimitra Alexopoulou, Andreas Dahl, Matthias Hackl, Johannes R. Schmidt, Stefan Kalkhof, Thomas Kurth, Hanna Taipaleenmaeki, Stefanie Conrad, Christine Hofbauer, Martina Rauner, Lorenz C. Hofbauer
Summary: Prostate cancer communicates with bone progenitor cells through extracellular vesicles, promoting vitality and proliferation of the progenitor cells while decreasing mineral deposition. Additionally, small RNAs released by prostate cancer cells may play a critical role in suppressing the activity of bone progenitor cells. These findings reveal an important communication mechanism between prostate cancer and bone.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Xiaowei Wei, Lijun Cai, Hanxu Chen, Luoran Shang, Yuanjin Zhao, Weijian Sun
Summary: This study proposes a novel system for multiplexed analysis of bladder cancer-derived urine exosomes using magnetic microspheres as barcoded microcarriers. The unique structure of the microcarriers allows for controllable movement and high sensitivity detection of exosomes. The noninvasive nature of the platform, combined with its good sensitivity, specificity, low sample consumption, and ease of use, indicates its great potential for bladder cancer diagnosis in clinical applications.
ANALYTICAL CHEMISTRY
(2022)
Article
Oncology
Marcelino Yazbek Hanna, Mark Winterbone, Shea P. O'Connell, Mireia Olivan, Rachel Hurst, Rob Mills, Colin S. Cooper, Daniel S. Brewer, Jeremy Clark
Summary: There is interest in using urine as a non-invasive liquid biopsy for detecting prostate cancer. The usefulness of PCa-specific transcripts in urine extracellular vesicles (EVs) and urine cell-sediment (Cell) has not been fully understood. A study analyzed urine samples from 76 men and found differential gene expression in EVs and Cells, indicating the importance of fractionating urine prior to analysis. However, there was no evidence that one fraction provided superior information over the other.
Article
Multidisciplinary Sciences
Riccardo Vago, Giorgia Radano, Davide Zocco, Natasa Zarovni
Summary: Urine is an ideal source of non-invasive diagnostic markers, however, issues such as urine concentration variability and biases introduced in urine EV analysis restrict its full potential. This study evaluated methods to decrease biases and found that appropriate urine storage, optimal single-step EV purification method, and normalization method can enable accurate analysis of urine EV RNA.
SCIENTIFIC REPORTS
(2022)
Review
Biochemistry & Molecular Biology
Karen Yamaguchi, Tomofumi Yamamoto, Junichiro Chikuda, Tatsuo Shirota, Yusuke Yamamoto
Summary: Drug resistance in oral cancer is a major issue due to therapeutic failure, with ncRNAs playing a crucial role in regulating gene expression and intercellular communication related to drug resistance. These ncRNAs could potentially serve as therapeutic targets and biomarkers for cancer diagnosis.
Article
Biochemistry & Molecular Biology
Allison Mathiesen, Bronson Haynes, Ryan Huyck, Michael Brown, Anca Dobrian
Summary: Metastatic prostate cancer is associated with obesity and systemic chronic inflammation. Adipose tissue inflammation leads to dysfunctional endothelial cells and the production of extracellular vesicles (EV), which can affect malignant cells in distant tissues. In this study, EVs from obese adipose tissue and inflamed endothelial cells were found to impact the phenotypic plasticity of prostate cancer cells, with reduced invasion and increased proliferation. These functional changes were attributed to the miRNA cargo of the EVs and were TWIST1-dependent.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Samanta Salvi, Erika Bandini, Silvia Carloni, Valentina Casadio, Michela Battistelli, Sara Salucci, Ilaria Erani, Emanuela Scarpi, Roberta Gunelli, Giacomo Cicchetti, Michele Guescini, Massimiliano Bonafe, Francesco Fabbri
Summary: Prostate Cancer (PCa) is a common urological cancer which leads to overdiagnosis due to lack of specific diagnostic methods. This study explores the use of extracellular vesicles (EVs) as potential markers for liquid biopsy, showing promising results in detecting physiological and pathological processes related to cancer. The research aims to develop a new workflow for investigating EVs from various biological sources, with significant alterations in EVs potentially related to PCa and BPH.
Article
Oncology
Susann Allelein, Keshia Aerchlimann, Gundula Roesch, Roxana Khajehamiri, Andreas Koelsch, Christian Freese, Dirk Kuhlmeier
Summary: Prostate cancer is the second most common cancer in men, which can be successfully treated when detected early. Communication between cells is done through the release of EVs, and specific isolation of EVs from urine can improve their surface markers and have potential clinical value.
Article
Biochemistry & Molecular Biology
Cinzia Giordano, Felice Maria Accattatis, Luca Gelsomino, Piercarlo Del Console, Balazs Gyorffy, Mario Giuliano, Bianca Maria Veneziani, Grazia Arpino, Carmine De Angelis, Pietro De Placido, Erica Pietroluongo, Francesco Zinno, Daniela Bonofiglio, Sebastiano Ando, Ines Barone, Stefania Catalano
Summary: This study identified novel miRNA signatures, miRNA-27a and miRNA-128, in breast cancer by analyzing the expression profiles of EV-derived miRNAs in the blood circulation. These miRNAs showed diagnostic accuracy in distinguishing breast cancer patients from non-cancer controls.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Yesim Verel-Yilmaz, Juan Pablo Fernandez, Agnes Schaefer, Sheila Nevermann, Lena Cook, Norman Gercke, Frederik Helmprobst, Christian Jaworek, Elke Pogge von Strandmann, Axel Pagenstecher, Detlef K. Bartsch, Joerg W. Bartsch, Emily P. Slater
Summary: The study aimed to determine whether ADAM8-positive extracellular vesicles (EVs) and cargo microRNAs could distinguish PDAC or its precursor lesions from healthy controls. FACS analysis and EV cargo analysis of miRNAs revealed a potential diagnostic biomarker set to discriminate PDAC and precursor lesions from healthy controls.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Rui Miguel Marques Bernardino, Ricardo Leao, Rui Henrique, Luis Campos Pinheiro, Prashant Kumar, Prashanth Suravajhala, Hans Christian Beck, Ana Sofia Carvalho, Rune Matthiesen
Summary: The study explores possible strategies for urinary biomarker discovery, especially focused on proteome of urine EVs for PCa. Through reanalysis of proteomics data and network analysis, the findings suggest that urinary EVs may provide potential biomarkers with higher protein coverage compared to other sample sources and may fully characterize the heterogeneity of PCa.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Urology & Nephrology
Fumihiko Urabe, Yusuke Yamamoto, Takahiro Kimura
Summary: Prostate cancer is the most common male cancer in Western Europe and North America. Intracellular and extracellular microRNAs play important roles in prostate cancer progression, with extracellular microRNAs showing potential as noninvasive diagnostic and prognostic biomarkers.
INTERNATIONAL JOURNAL OF UROLOGY
(2022)
Article
Cell Biology
Lidia Zabegina, Inga Nazarova, Nadezhda Nikiforova, Maria Slyusarenko, Elena Sidina, Margarita Knyazeva, Evgenia Tsyrlina, Sergey Novikov, Sergey Reva, Anastasia Malek
Summary: Prostate cancer-specific small extracellular vesicles (SEVs) containing miRNA can serve as a promising diagnostic marker. Functionalization of super-paramagnetic beads (SPMBs) with PSMA-binding DNA aptamer enhances the capture efficacy of PSMA(+)SEVs, leading to more sensitive detection of prostate cancer-related miRNAs in a smaller sample size. This method shows higher diagnostic potency compared to total SEV population analysis, suggesting its promise as a prostate cancer diagnosis tool.
Article
Gastroenterology & Hepatology
Qichen Shen, Zhuizui Huang, Lingyan Ma, Jiachen Yao, Ting Luo, Yao Zhao, Yingping Xiao, Yuanxiang Jin
Summary: Inflammatory bowel disease (IBD) is a global disease without a cure, and disruption of the microbial ecosystem is considered a major cause. This study investigated the role of extracellular vesicles (EVs) and membrane vesicles (MVs) in host-microbe interactions in colitis. The researchers found that miR-181b-5p transplantation inhibited inflammation and promoted macrophage polarization, while miR-200b-3p regulated the microbiota composition and contributed to intestinal barrier integrity. MVs from feces and BMVs from colitis also had similar effects. These findings provide new insights and potential treatment strategies for colitis.
Article
Engineering, Biomedical
Vera S. Chernonosova, Petr P. Laktionov, Ivan S. Murashov, Andrey A. Karpenko, Pavel P. Laktionov
BIOMEDICAL MATERIALS
(2020)
Article
Cardiac & Cardiovascular Systems
Y. Ragino, E. Kashtanova, I. S. Murashov, A. M. Volkov, A. Kurguzov, E. Sadovski, N. A. Maslatsov, L. Scherbakova, A. M. Chernjavskii, Y. Polonskaya
Article
Polymer Science
Konstantin A. Kuznetsov, Ivan S. Murashov, Vera S. Chernonosova, Boris P. Chelobanov, Alena O. Stepanova, David S. Sergeevichev, Andrey A. Karpenko, Pavel P. Laktionov
Article
Medicine, General & Internal
Maria Yu. Konoshenko, Olga E. Bryzgunova, Evgeniy A. Lekchnov, Evgeniya V. Amelina, Sergey V. Yarmoschuk, Svetlana V. Pak, Pavel P. Laktionov
Article
Medicine, General & Internal
Yana V. Polonskaya, Elena V. Kashtanova, Ivan S. Murashov, Aleksei V. Kurguzov, Evgeny V. Sadovski, Nikolay A. Maslatsov, Ekaterina M. Stakhneva, Alexander M. Chernyavskii, Yuliya I. Ragino
Article
Clinical Neurology
Vladimir S. Klimov, Vladislav V. Kelmakov, Brent L. Clyde, Aleksey Evsyukov, Evgeniy A. Loparev, Evgenia Amelina, Anatoliy Bervitskiy, Anton P. Ponomarenko, Jamil A. Rzaev
Summary: This study aimed to evaluate the long-term clinical and radiological outcomes of anterior uncoforaminotomy in patients with degenerative cervical spine pathology. The results showed improvement in clinical symptoms after surgery with no significant complications, and the stability of the cervical spine was maintained.
Article
Clinical Neurology
Galina Moisak, Evgeniya Amelina, Nadejda A. Zubok, Jamil A. Rzaev
Summary: The study found that a portion of patients with trigeminal neuralgia experience anxiety and depression, with most patients not rating the intervention as excellent despite pain relief post-surgery.
CLINICAL NEUROLOGY AND NEUROSURGERY
(2021)
Article
Chemistry, Physical
Vera Chernonosova, Alexandr Gostev, Ivan Murashov, Boris Chelobanov, Andrey Karpenko, Pavel Laktionov
Summary: This study examined the properties and compatibility of electrospun 3D matrices for vascular grafts produced using different materials. The results showed good hemocompatibility of scaffolds with Gel and bivalirudin, as well as the advantages of Pel-80A grafts over ePTFE in terms of graft occlusion and calcification levels. Additionally, the introduction of bivalirudin into grafts inhibited platelet adhesion.
Article
Health Care Sciences & Services
Yana Polonskaya, Elena Kashtanova, Ivan S. Murashov, Evgenia Striukova, Alexey Kurguzov, Ekaterina M. Stakhneva, Viktoria S. Shramko, Nikolay A. Maslatsov, Aleksandr M. Chernyavsky, Yulia Ragino
Summary: The study investigated the relationship between matrix metalloproteinases and coronary artery calcification. Results showed the involvement of some MMPs, particularly MMP-9, in the calcification process. MMP-1, MMP-7, and MMP-12 were associated with CHD and may serve as potential biomarkers for further research.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Cardiac & Cardiovascular Systems
Ilya A. Soynov, Igor A. Kornilov, Yuriy Y. Kulyabin, Alexey V. Zubritskiy, Dmitry N. Ponomarev, Nataliya R. Nichay, Ivan S. Murashov, Alexander V. Bogachev-Prokophiev
Summary: The study showed that additional diagnostic tests (such as CT scans or cardiac catheterization) during ECMO support for pediatric patients after cardiac surgery can better identify residual lesions and improve survival rates.
WORLD JOURNAL FOR PEDIATRIC AND CONGENITAL HEART SURGERY
(2021)
Article
Biochemistry & Molecular Biology
Evgeniia V. Garbuzova (Striukova), Victoriya S. Shramko, Elena V. Kashtanova, Yana V. Polonskaya, Ekaterina M. Stakhneva, Alexey V. Kurguzov, Ivan S. Murashov, Alexander M. Chernyavsky, Yuliya I. Ragino
Summary: The study aimed to investigate the levels of adipokines and their associations with unstable atherosclerotic plaques in patients with coronary atherosclerosis and abdominal obesity (AO). The findings showed that patients with AO and unstable plaques had higher levels of GLP-1 and lower levels of lipocalin-2. Lipocalin-2 was inversely associated with unstable plaques. The conclusion is that GLP-1 is directly associated with AO, while lipocalin-2 is inversely associated with unstable plaques in patients with AO.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
E. M. Stakhneva, E. V. Kashtanova, A. V. Kurguzov, N. A. Maslatsov, Y. V. Polonskaya, I. S. Murashov, A. M. Chernyavskii, V. S. Shramko, Y. I. Ragino
Meeting Abstract
Cardiac & Cardiovascular Systems
E. V. Striukova, E. Shakhtshneider, D. Ivanoshchuk, Y. I. Ragino, Y. V. Polonskaya, I. S. Murashov, A. M. Volkov, A. V. Kurguzov, A. Chernyavsky, E. Valeev, V. N. Maksimov, E. V. Kashtanova
Meeting Abstract
Cardiac & Cardiovascular Systems
Y. Polonskaya, E. Kashtanova, V. Shramko, E. Striukova, A. Kurguzov, I. Murashov, Y. Ragino
Article
Genetics & Heredity
E. V. Striukova, V. N. Maksimov, Yu. I. Ragino, Ya. V. Polonskaya, I. S. Murashov, A. M. Volkov, A. V. Kurguzov, A. M. Chernjavskii, V. Kashtanova
Summary: The C allele of the single-nucleotide polymorphism rs708272 of the CETP gene is associated with the presence of unstable atherosclerotic plaques in the coronary arteries. The study did not find significant differences in the APOC3 and APOE genes. Different genotypes of CETP and APOC3 were associated with varying levels of TNF-alpha, factor XII, sVCAM-1, and IL-8.