Article
Microbiology
Michelle Qiu Carter, Beatriz Quinones, Xiaohua He, Antares Pham, Diana Carychao, Michael B. Cooley, Chien-Chi Lo, Patrick S. G. Chain, Rebecca L. Lindsey, James L. Bono
Summary: Through comparative genomics and phenotypic characterization, this study assessed the pathogenic potential of Escherichia albertii strains isolated from wild birds in California. The results showed that these strains have high pathogenicity potential and could cause severe diseases in humans.
Article
Immunology
Zhen Yang, Yiwen Zhang, Senyan Du, Qin Zhao, Xiaobo Huang, Rui Wu, Qigui Yan, Xinfeng Han, Sanjie Cao, Yung-Fu Chang, Yiping Wen
Summary: Highly virulent strain G. parasuis SC1401 stimulated tight junction expression, resulting in higher bacterial adhesion to respiratory tract cells, which may be related to CDT.
INFECTION AND IMMUNITY
(2023)
Article
Immunology
Jayedul Hassan, Sharda Prasad Awasthi, Noritoshi Hatanaka, Phuong Hoai Hoang, Akira Nagita, Atsushi Hinenoya, Shah M. Faruque, Shinji Yamasaki
Summary: In this study, we found cdt genes in P. rustigianii strain, which showed over 90% homology to P. alcalifaciens strain at nucleotide and amino acid sequence levels. The P. rustigianii strain produced biologically active CDT toxin.
INFECTION AND IMMUNITY
(2023)
Article
Microbiology
Anthony Mannion, Whitney McGee, Yan Feng, Zeli Shen, Ellen Buckley-Jordan, JoAnn L. Dzink-Fox, James G. Fox
Summary: This study discovered the presence of ExPEC E. coli infection in SPF cats with a history of reproductive failure, suggesting a possible association with impaired reproductive health and breeding success. Following treatment with cefovecin, reproductive performance significantly improved.
VETERINARY MICROBIOLOGY
(2022)
Review
Immunology
Yi-Ru Lai, Yu-Fang Chang, Jason Ma, Cheng-Hsun Chiu, Ming-Ling Kuo, Chih-Ho Lai
Summary: Recent studies have shown that bacteria producing CDT target host DNA, potentially contributing to cancer development. Understanding the mechanisms of genotoxins causing DNA damage may help identify potential markers that drive cancer progression and discover new therapeutic strategies to prevent diseases caused by pathogens.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Food Science & Technology
Maryam Keshtvarz, Mahdieh Mahboobi, Marek Kieliszek, Antoni Miecznikowski, Hamid Sedighian, Milad Rezaei, Mohammad Ali Haghighi, Zahra Zareh, Ehsan Rezaei
Summary: The cytolethal distending toxin (CDT) is a bacterial toxin considered for targeted therapies, with its catalytic subunit CdtB capable of inducing DNA double-strand breaks and cell apoptosis. Reduction of CdtB immunogenicity through mutagenesis can enhance stability and enable its use in toxin-based drugs like immunotoxins.
Article
Immunology
Jiayun Gu, Yan Lin, Zhichao Wang, Qicong Pan, Guohua Cai, Qigai He, Xiaojuan Xu, Xuwang Cai
Summary: This study elucidates the molecular mechanism by which CDT induces pyroptosis via the ROS/caspase-9/caspase-3/GSDME signaling pathway.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Miao Qian, Shuyang Cao, Tao Wang, Xiangming Xu, Quan Zhang
Summary: In this study, it was found that bacterial toxin CdtB promotes the expression of inflammatory cytokines, decreases cell viability, and induces cell apoptosis. Moreover, CdtB triggers apoptosis through the MAPK/Erk/p38 signaling pathway and is inhibited by autophagy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Cell Biology
G. Robb Huhn, Naly Torres-Mangual, John Clore, Lucia Cilenti, Teresa Frisan, Ken Teter
Summary: Gram-negative pathogens produce cytolethal distending toxin (CDT) with two cell-binding subunits (CdtA + CdtC) and a catalytic CdtB subunit. After adhesion to the plasma membrane, CDT moves to the endoplasmic reticulum and CdtB enters the nucleus to cause DNA breaks. Our study re-examined the potential endocytosis of CdtA from Haemophilus ducreyi CDT and found rapid degradation of internalised CdtA, CdtB, and CdtC. Internalised CdtA was visualised in early endosomes by confocal microscopy.
CELLULAR MICROBIOLOGY
(2021)
Article
Nutrition & Dietetics
Alexandra Adorno Vita, Heather Zwickey, Ryan Bradley
Summary: The study found significant associations between food-specific IgG antibodies and intestinal permeability biomarkers in adults, particularly antibodies against LPS and occludin. While levels of IgG antibodies and permeability biomarkers did not increase in symptomatic patients, the presence of multiple IgG antibodies and increasing titers can be indicative of higher levels of antibodies.
FRONTIERS IN NUTRITION
(2022)
Article
Cell Biology
Jia-Yin Yeh, Hwai-Jeng Lin, Chia-Jung Kuo, Chun-Lung Feng, Chia-Huei Chou, Chia-Der Lin, Hui-Yu Wu, Chen-Yi Li, Cheng-Hsun Chiu, Chih-Ho Lai
Summary: Using recombinant CdtC can alleviate the effects of H. pylori cytotoxins in cells, mitigating H. pylori-induced gastric epithelial cell inflammation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Gastroenterology & Hepatology
Danielle D. Munhoz, Amanda C. Richards, Fernanda F. Santos, Matthew A. Mulvey, Roxane M. F. Piazza
Summary: Pathogenic subsets of Escherichia coli include diarrheagenic (DEC) strains and extraintestinal pathogenic E. coli (ExPEC) strains. The newly reclassified hybrid strain BA1250 possesses genetic features of both DEC and ExPEC and has the potential to cause disease outside of the gut. The adhesin E. coli common pilus (ECP) acts as a colonization and virulence factor for BA1250, and changes in its activation may affect the fitness of the strain.
Article
Medicine, General & Internal
Riccardo Vasapolli, Christian Schulz, Melanie Schweden, Christina Casen, Graceline Tina Kirubakaran, Katrine Hanes Kirste, Lukas Macke, Alexander Link, Kerstin Schuette, Peter Malfertheiner
Summary: This study found that the positivity rate of anti-CdtB and anti-vinculin antibodies was higher in patients with constipation-type IBS, who also exhibited more dysbiosis with increased opportunistic/pro-inflammatory bacteria and reduced gut protective bacteria. Microbial profiles of patients with diarrhoea-type IBS and functional dyspepsia were similar to those of healthy controls.
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Public, Environmental & Occupational Health
Pedro Guirado, Yaidelis Iglesias-Torrens, Elisenda Miro, Ferran Navarro, Camile Stephan-Otto Attolini, Carlos Balsalobre, Cristina Madrid
Summary: Campylobacter is a major cause of food-borne gastroenteritis worldwide and mainly colonizes the gastrointestinal tract of birds. The mechanisms of Campylobacter's interaction with different hosts are not well understood. This study characterized the allelic variability of the cdtABC operon in Campylobacter isolates from wild birds, broiler chickens, and humans. The results suggest that specific variants of the cdtABC operon might determine the host range of Campylobacter jejuni isolates. Additionally, the accuracy of PCR methodology in detecting cdt genes is questioned.
ZOONOSES AND PUBLIC HEALTH
(2022)
Article
Immunology
Grace Huang, Kathleen Boesze-Battaglia, Lisa P. Walker, Ali Zekavat, Zachary P. Schaefer, Steven R. Blanke, Bruce J. Shenker
Summary: The CdtB subunits from Aggregatibacter actinomycetemcomitans, Haemophilus ducreyi, and Campylobacter jejuni exhibit potent PIP3 phosphatase activity, leading to PI-3K signaling blockade in treated cells. This blockade is essential for Cdts-induced toxicity in lymphocytes, which can be reversed by GSK3 beta inhibitors. Furthermore, Cdts from HdCdt and CjCdt, like AaCdt, rely on host cell protein cellugyrin for their toxicity.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Veterinary Sciences
Azimun Nahar, Sharda Prasad Awasthi, Noritoshi Hatanaka, Kentaro Okuno, Phuong Hoai Hoang, Jayedul Hassan, Atsushi Hinenoya, Shinji Yamasaki
JOURNAL OF VETERINARY MEDICAL SCIENCE
(2018)
Article
Biotechnology & Applied Microbiology
Sucharit Basu Neogi, Nityananda Chowdhury, Sharda Prasad Awasthi, Masahiro Asakura, Kentaro Okuno, Zahid Hayat Mahmud, Mohammad Sirajul Islam, Atsushi Hinenoya, Gopinath Balakrish Nair, Shinji Yamasaki
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2019)
Article
Immunology
Jayedul Hassan, Sharda Prasad Awasthi, Noritoshi Hatanaka, Kentaro Okuno, Phuong Hoai Hoang, Akira Nagita, Atsushi Hinenoya, Shinji Yamasaki
PATHOGENS AND DISEASE
(2018)
Article
Biochemical Research Methods
Sharda Prasad Awasthi, Nityananda Chowdhury, Sucharit Basu Neogi, Atsushi Hinenoya, Noritoshi Hatanaka, Goutam Chowdhury, T. Ramamurthy, Shinji Yamasaki
JOURNAL OF MICROBIOLOGICAL METHODS
(2019)
Article
Infectious Diseases
Atsushi Hinenoya, Hidetoshi Ichimura, Noritomo Yasuda, Seiya Harada, Kazuhiro Yamada, Masahiro Suzuki, Yoshio Iijima, Akira Nagita, M. John Albert, Noritoshi Hatanaka, Sharda Prasad Awasthi, Shinji Yamasaki
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2019)
Article
Infectious Diseases
Atsushi Hinenoya, Keigo Nagano, Kentaro Okuno, Akira Nagita, Noritoshi Hatanaka, Sharda Prasad Awasthi, Shinji Yamasaki
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2020)
Article
Food Science & Technology
Noritoshi Hatanaka, Sharda Prasad Awasthi, Hisataka Goda, Hiroyuki Kawata, Yuzuru Uchino, Takahiro Kubo, Shigeru Aoki, Atsushi Hinenoya, Shinji Yamasaki
Article
Biochemical Research Methods
Noritoshi Hatanaka, Sharda Prasad Awasthi, Atsushi Hinenoya, Osamu Ueda, Shinji Yamasaki
JOURNAL OF MICROBIOLOGICAL METHODS
(2020)
Article
Food Science & Technology
Rabee A. Ombarak, Sharda Prasad Awasthi, Noritoshi Hatanaka, Shinji Yamasaki
Summary: The study identified an ESBL-producing E. coli isolate carrying the mcr-1 gene on a plasmid, with potential transferability. The isolate exhibited multidrug resistance, including resistance to colistin, and harbored additional resistance genes. The mcr-1 gene was successfully transferred to another E. coli strain through conjugation, indicating the potential spread of this gene to other Gram-negative bacteria.
INTERNATIONAL DAIRY JOURNAL
(2021)
Article
Microbiology
Sharda Prasad Awasthi, Nityananda Chowdhury, Noritoshi Hatanaka, Atsushi Hinenoya, Thandavarayan Ramamurthy, Masahiro Asakura, Shinji Yamasaki
Summary: A sensitive bead-ELISA assay was developed to quantify ChxA produced by V. cholerae, showing that ChxA is predominantly produced extracellularly in cell-free supernatant at varying levels depending on strains and culture conditions. V. cholerae strains with high ChxA II production exhibited higher lethality in a mouse model assay.
JOURNAL OF MEDICAL MICROBIOLOGY
(2021)
Article
Immunology
Jayedul Hassan, Sharda Prasad Awasthi, Noritoshi Hatanaka, Phuong Hoai Hoang, Akira Nagita, Atsushi Hinenoya, Shah M. Faruque, Shinji Yamasaki
Summary: In this study, we found cdt genes in P. rustigianii strain, which showed over 90% homology to P. alcalifaciens strain at nucleotide and amino acid sequence levels. The P. rustigianii strain produced biologically active CDT toxin.
INFECTION AND IMMUNITY
(2023)
Article
Immunology
Atsushi Hinenoya, Keigo Nagano, Sharda P. Awasthi, Noritoshi Hatanaka, Shinji Yamasaki
EMERGING INFECTIOUS DISEASES
(2020)
Article
Infectious Diseases
Rabee Alhossiny Ombarak, Mahmoud Gamaleldin Zayda, Sharda Prasad Awasthi, Atsushi Hinenoya, Shinji Yamasaki
JAPANESE JOURNAL OF INFECTIOUS DISEASES
(2019)
Article
Veterinary Sciences
Atsushi Hinenoya, Suong Thi Thu Tran, Ngu Trong Nguyen, Ha Cong Nguyen, Doan Duy Le Nguyen, Phuong Hoai Hoang, Sharda Prasad Awasthi, Jayedul Hassan, Yoshinori Sumimura, Yoshimasa Yamamoto, Shinji Yamasaki
JAPANESE JOURNAL OF VETERINARY RESEARCH
(2018)