期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 121, 期 -, 页码 852-861出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2018.10.091
关键词
Antihypertensive; Antioxidant; Crassostrea gigas; Hypoglycemic activity; Polysaccharides; Subcritical water extraction
资金
- Korea Technology and Information Promotion Agency for SMEs (TIPA) grant - Korea government (Ministry of SMEs and Startups) [C0550312]
- Korea Technology & Information Promotion Agency for SMEs (TIPA) [C0550312] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Bioactive polysaccharide was extracted from Pacific oyster Crassostrea gigas using subcritical water (SW) and the extraction process was optimize by response surface methodology (RSM). The optimum condition was found to be temperature 125.01 degrees C, extraction time 14.93 min, and liquid to solid ratio of 44.69:1 (ml:g). At this condition, the yield of the C gigas polysaccharides (CGPs) was found to be 18.66%. The polysaccharide was characterized for its chemical, physical, thermal, and structural properties using HPLC, GPC, XRD, FTIR, TGA, UV-Vis spectroscopy, and NMR and the results of this characterization showed a characteristic feature of a typical polysaccharide. The CGPs was found to be a o-glucan with of-(1-04) configuration. The CGPs was also evaluated for its antioxidant, antihypertensive, and hypoglycemic activity and the IC50 (mg/ml) values were found to be 2.06 +/- 033, 1.58 0.03, and 2.77 0.01 respectively. The current study demonstrated that SWE could be used as an effective process to extract bioactive polysaccharides from C gigas. (C) 2018 Elsevier B.V. All rights reserved.
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