期刊
GENOME RESEARCH
卷 29, 期 1, 页码 18-28出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gr.238527.118
关键词
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资金
- Marie-Curie Cofund program fellowship (EU)
- Juan de la Cierva fellowship
- Spanish Ministry of Economy and Competitiveness [BFU2015-64437-P, BFU2014-52125-REDT, BFU2014-51672-REDC, BFU2017-85152-P]
- Catalan Government [2017 SGR 799]
- Fundacion Botin
- Banco Santander through its Santander Universities Global Division
- Unidad de Excelencia Maria de Maeztu [MDM-2014-0370]
- ICREA Academia (Generalitat de Catalunya)
- European Research Council (ERC Synergy Grant 4D Genome) [609989]
- CERCA
- Beatriu de Pinos (Generalitat de Catalunya)
Nuclear architecture is decisive for the assembly of transcriptional responses. However, how chromosome organization is dynamically modulated to permit rapid and transient transcriptional changes in response to environmental challenges remains unclear. Here we show that hyperosmotic stress disrupts different levels of chromosome organization, ranging from A/B compartment changes to reduction in the number and insulation of topologically associating domains (TADs). Concomitantly, transcription is greatly affected, TAD borders weaken, and RNA Polymerase II runs off from hundreds of transcription end sites. Stress alters the binding profiles of architectural proteins, which explains the disappearance of local chromatin organization. These processes are dynamic, and cells rapidly reconstitute their default chromatin conformation after stress removal, uncovering an intrinsic organization. Transcription is not required for local chromatin reorganization, while compartment recovery is partially transcription-dependent. Thus, nuclear organization in mammalian cells can be rapidly modulated by environmental changes in a reversible manner.
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