Article
Biochemistry & Molecular Biology
Chuanqi Wang, Defu Yao, Mingming Zhao, Kaiyu Lu, Zhongyang Lin, Xiuli Chen, Yongzhen Zhao, Yueling Zhang
Summary: This study found that Vp (AHPND) infection in cultured shrimp increased the expression of lipid droplet protein LvPerilipin and promoted the formation of lipid droplets. Knockdown of LvPerilipin increased shrimp survival rate and inhibited Vp (AHPND) proliferation. Furthermore, depletion of LvPerilipin increased the production of reactive oxygen species, which may be responsible for the decreased Vp (AHPND) proliferation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Fisheries
Thao Nguyen, Andrea Alfaro, Bonny Bayot Arroyo, Jenny Antonia Rodriguez Leon, Stanislaus Sonnenholzner
Summary: The study used a metabolomics approach to measure molecular responses of shrimp exposed to Vibrio parahaemolyticus, revealing an increase in metabolic rate, elevated TCA cycle, and gluconeogenesis activities in infected shrimp. This provides important new findings about the pathogenic mechanisms of V. parahaemolyticus invasion in shrimp at the metabolic level.
Article
Immunology
Dunhua Zhang, Troy J. Bader, Miles D. Lange, Craig A. Shoemaker, Benjamin H. Beck
Summary: The study identified PirA and PirB as the major virulence factors causing AHPND and found that their interactions enhanced the toxic effect.
MICROBIAL PATHOGENESIS
(2021)
Article
Fisheries
Thuy-Dung Mai-Hoang, Hai-Ly Tien, Hai-Minh Chau-Hoang, Khai-Hoan Nguyen-Phuoc, Hung Quoc Pham, Thuoc Linh Tran, Hieu Tran-Van
Summary: AHPND is a global threat, especially for shrimp culture in Vietnam, caused by Vibrio parahaemolyticus carrying toxins with a mortality rate of up to 100% post infection. A mutant AHPND with a transposon insertion was recorded, but currently there is no commercially available PCR test to detect both normal and mutant plasmids simultaneously.
Article
Microbiology
Ramya Kumar, Teng-Chun Tung, Tze Hann Ng, Che-Chih Chang, Yi-Lun Chen, Yi-Min Chen, Shih-Shun Lin, Han-Ching Wang
Summary: The study revealed that AHPND infection can impact lipid metabolism in shrimp stomach, and that the primary bile acid taurocholate is crucial for the virulence of AHPND-causing Vibrio parahaemolyticus.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Fisheries
Qian Zhang, Yang Yu, Zheng Luo, Fuhua Li
Summary: In this study, the correlation between hepatopancreas color and the infection process of V. parahaemolyticus was analyzed to find a convenient method for evaluating shrimp resistance to AHPND. The hepatopancreas color changes can reflect the stages of infection process, and help us evaluate shrimp resistance to Vibrio, accelerating the breeding of disease-resistant shrimp.
Article
Fisheries
Vikash Kumar, Kartik Baruah, Peter Bossier
Summary: The study found that bamboo powder can enhance the survival of brine shrimp larvae against AHPND-causing Vibrio parahaemolyticus strains and induce phenotypic changes in these strains. By inducing phenotype switching, bamboo powder might partially protect brine shrimp larvae against AHPND-causing Vibrio parahaemolyticus.
Article
Fisheries
Tran Huu Tinh, Sivaramasamy Elayaraja, Mahmoud Mabrok, Putu Cri Devischa Gallantiswara, Varaporn Vuddhakul, Channarong Rodkhum
Summary: This study investigated the potential use of synthetic herbal-based polyphenols compounds as natural antimicrobial agents against VP causing AHPND. The results showed that vanillic acid and pyrogallol exhibited strong antibacterial activity, with pyrogallol being suggested as a potential antimicrobial agent for the control of AHPND in shrimp producing sectors.
Article
Fisheries
K. G. Aguilar-Rendon, S. A. Soto-Rodriguez, B. Gomez-Gil, R. Lozano-Olvera, B. Yanez-Rivera
Summary: This study investigated the impact of pathogenic strains causing acute hepatopancreatic necrosis disease (AHPND) on the water microbiome. The results showed that the virulence of the strains and the duration of the experiment could alter the water microbiome, particularly leading to a significant reduction in the reads assigned to Neptuniibacter spp. This depletion suggests a possible dual function of the type VI secretion system 1 (T6SS1) in the pathogenic strains, killing surrounding bacteria and damaging the hepatopancreas.
Article
Fisheries
Haonan Sha, Luyue Li, Jiaqi Lu, Jinbo Xiong
Summary: Shrimp diseases often occur in eutrophic farming water. Research has found that both oligotrophic and eutrophic infections of Vibrio parahaemolyticus, a pathogen causing shrimp acute hepatopancreatic necrosis disease (AHPND), can disrupt the gut microbiota and compromise shrimp immunity. The deterministic assembly of gut microbiota is stronger in eutrophic infections, and there is a more stable gut microbiota in shrimp infected with eutrophic Vibrio parahaemolyticus.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Tran Thi Tuyet Hoa, Mahougnon Simeon Fagnon, Dang Thuy Mai Thy, Thibaut Chabrillat, Nguyen Bao Trung, Sylvain Kerros
Summary: This study assessed the effect of a essential oil mixture on the growth performance and mortality of shrimps. The results showed that the mixture improved growth performance and significantly reduced mortality when challenged against a pathogenic bacteria.
Article
Immunology
Zhou Zheng, Shangjie Liu, Zhongyang Lin, Jude Juventus Aweya, Zhihong Zheng, Yongzhen Zhao, Xiuli Chen, Shengkang Li, Yueling Zhang
Summary: This study reveals that a transcription factor called PvKr-h1 is significantly induced in shrimp hemocytes after being challenged with Vibrio parahaemolyticus (AHPND), suggesting its involvement in shrimp immune response. Knockdown of PvKr-h1 followed by V. parahaemolyticus (AHPND) challenge leads to increased bacterial abundance in shrimp hemolymph and high shrimp mortality. Furthermore, the downregulation of PvKr-h1 dysregulates the expression of antioxidant-related enzymes and antimicrobial peptide genes, reduces ROS activity, and inhibits the nuclear translocation of Relish, thereby impairing shrimp's immune response against V. parahaemolyticus (AHPND) infection.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Food Science & Technology
Sonia A. Soto-Rodriguez, Rodolfo Lozano-Olvera, Gabriela Ramos-Clamont Montfort, Edgar Zenteno, Jose Luis Sanchez-Salgado, Norberto Vibanco-Perez, Karla G. Aguilar Rendon
Summary: The PirAB toxins secreted by Vibrio parahaemolyticus (Vp) play a role in causing acute hepatopancreatic necrosis disease (AHPND) in Penaeid shrimp. These toxins damage the epithelial cells of the shrimp hepatopancreas (Hp) and the PirB(Vp) subunit has lectin-like activity. Understanding the toxin receptors can help prevent host disease by blocking toxin-receptor interactions. Additionally, Vp AHPND changes the microbiota in the surrounding water, resulting in a reduction of certain bacterial taxa.
Article
Microbiology
Yi-Ting Chang, Hao-Ting Ko, Ping-Lun Wu, Ramya Kumar, Han-Ching Wang, Hsiao-Pei Lu
Summary: This study revealed that shrimp gut microbiota responded differently to pathogenic and non-pathogenic Vibrio parahaemolyticus strains, with pathogenic Vibrio-infected shrimp showing enrichment of Photobacterium and Vibrio genera, while non-pathogenic Vibrio-infected shrimp had Candidatus Bacilliplasma enriched. Functional predictions suggested that changes in taxonomic compositions would affect normal metabolic functions, highlighting the importance of maintaining gut microbiota equilibrium. Several biomarkers associated with specific microbial taxa and functional pathways were identified, aiding in predicting disease outcomes.
MICROBIOLOGY SPECTRUM
(2023)
Article
Immunology
Zhou Zheng, Ruiwei Li, Jude Juventus Aweya, Defu Yao, Fan Wang, Shengkang Li, Tran Ngoc Tuan, Yueling Zhang
Summary: The study demonstrates that in addition to affecting the digestive system of shrimp, the PirB toxin protein interacts with histones to influence the phosphorylation of histone H3-S10, thereby inducing apoptosis.
Article
Genetics & Heredity
Wafaa Ragab, Satoshi Kawato, Reiko Nozaki, Hidehiro Kondo, Ikuo Hirono
Summary: This study presents a comparative genome analysis of Vibrio penaeicida, revealing its genetic features, evolution, and pathogenicity. The results indicate that the strains belong to the same species and possess typical virulence factors and other related genes. These findings are important for understanding the pathogenesis of Vibrio penaeicida.
MICROBIAL GENOMICS
(2022)
Article
Virology
Satoshi Kawato, Shoya Fujishima, Reiko Nozaki, Hidehiro Kondo, Tadashi Isshiki, Ikuo Hirono
Summary: This study reports the causative agent of milky hemolymph disease in snow crab, known as Chionoecetes opilio bacilliform virus (CoBV), and reveals its complete genome sequence, confirming its classification as a member of the Nimaviridae family.
ARCHIVES OF VIROLOGY
(2023)
Article
Fisheries
Liu Wang, Keisuke Yoshii, Naoya Murase, Hidetoshi Yamada, Yutaka Fukuda, Ikuo Hirono, Hidehiro Kondo
Summary: This study aimed to develop a better immunogenic and protective fish vaccine and compare the effectiveness of poly I:C and formalin-killed cells (FKCs) as adjuvants against pathogen infection. The results showed that co-inoculation with poly I:C and FKC significantly increased the expression levels of specific antibodies and provided better protection compared to FKC vaccine alone.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Mahmoud Mostafa Mahmoud, Mohamed Abdelsalam, Satoshi Kawato, Shogo Harakawa, Hidemasa Kawakami, Ikuo Hirono, Hidehiro Kondo
Summary: This study compared the whole genome sequences of three different serotypes of Lactococcus garvieae isolated from diseased cultured striped jack in Ehime prefecture, Japan. The serotypes showed different virulence in a challenge test. Two serotypes were identified as L. garvieae and one as L. formosensis. The genome sizes ranged from 1.9 to 2.0 Mb, GC content ranged from 38.2% to 38.9%, and the number of predicted protein-coding sequences ranged from 1922 to 1959. Only one serotype harbored two plasmids.
JOURNAL OF FISH DISEASES
(2023)
Article
Fisheries
Jayasundara Mudiyanselage Sajani Nisansala Rathnapala, Wafaa Ragab, Satoshi Kawato, Miho Furukawa, Reiko Nozaki, Hidehiro Kondo, Ikuo Hirono
Summary: By sequencing the genomes of three strains of Vibrio nigripulchritudo, it was found that they possess various virulence factors and mobile genetic elements, as well as multidrug resistance. Comparative genomic analysis showed that all three strains belong to the same clade.
JOURNAL OF FISH DISEASES
(2023)
Article
Biotechnology & Applied Microbiology
Keiichiro Koiwai, Hidehiro Kondo, Ikuo Hirono
Summary: In this study, Drop-seq technology was used to investigate the impact of virus infection on hemocyte populations in shrimp. The findings revealed that virus infection reduced specific cell populations in circulating hemolymph and inhibited the expression of antimicrobial peptides. Unknown genes with antimicrobial activity were also identified. Additionally, the study discussed the effects of methanol fixation on Drop-seq data.
MARINE BIOTECHNOLOGY
(2023)
Article
Fisheries
Chalermkwan Eurlaphan, Reiko Nozaki, Motohiko Sano, Keiichiro Koiwai, Ikuo Hirono, Hidehiro Kondo
Summary: This study investigated the kinetics of red sea bream iridovirus and host gene expression during infection in rock bream (Oplegnathus fasciatus). The results showed that viral gene transcripts were detected at 3 and 5 days post-injection, and certain host genes were differentially expressed during the infection process. The downregulation of certain inflammation-related genes may contribute to the increased susceptibility of this fish to the virus.
JOURNAL OF FISH DISEASES
(2023)
Article
Fisheries
Hidehiro Kondo, Haruka Kamei, Fuyuka Murotani, Reiko Nozaki, Ryosuke Yazawa, Reoto Tani, Keiichiro Koiwai, Ikuo Hirono
Summary: A novel immunoglobulin heavy chain-like gene (tsIgH) was identified in the liver of the banded houndshark Triakis scyllium through preliminary transcriptomic analysis. The tsIgH gene had low amino acid sequence similarities (< 30%) to shark Ig genes. It encoded one variable domain (VH) and three conserved domains (CH1-CH3) with a predicted signal peptide. The gene structure and expression pattern suggest that this gene may provide insights into the evolution of immunoglobulin genes in cartilaginous fishes.
FISH AND SHELLFISH IMMUNOLOGY REPORTS
(2023)
Article
Fisheries
Hiroaki Saito, Shungo Minami, Manami Yuguchi, Aiko Shitara, Hidehiro Kondo, Goshi Kato, Motohiko Sano
Summary: The live attenuated vaccine strain P7-P8 demonstrates high protective efficacy against herpesviral haematopoietic necrosis caused by cyprinid herpesvirus 2 (CyHV-2) in goldfish at temperatures ranging from 15 to 30 degrees C. The vaccine virus load over 104 DNA copies/mg may elicit sufficient acquired immunity, and cell-mediated immunity may play a fundamental role in protection.
JOURNAL OF FISH DISEASES
(2023)
Article
Microbiology
Satoshi Kawato, Reiko Nozaki, Hidehiro Kondo, Ikuo Hirono
Summary: In this study, the genomes of 27 endogenous nimaviruses were recovered from crustacean genome data. Phylogenetic analysis revealed different lineages and specific insertion preferences for each nimavirus species. These findings suggest that endogenous nimaviruses are giant transposable elements occupying different sequence niches.
MICROBIOLOGY SPECTRUM
(2023)