Article
Agriculture, Dairy & Animal Science
Ga Yeon Kim, Gyu Tae Choi, Jinryong Park, Jeongeun Lee, Jeong Tae Do
Summary: In this study, two types of porcine MSCs were compared, and it was found that adipose-derived stem cells showed better proliferation capacity, adipogenic and chondrogenic differentiation potential, and mitochondrial metabolism compared to Wharton's jelly-derived mesenchymal stem cells. This study provides insights into the characteristics of porcine MSCs and their potential applications in animal clinics and cultured meat science.
Article
Veterinary Sciences
Min-Soo Seo, Kyung-Ku Kang, Se-Kyung Oh, Soo-Eun Sung, Kil-Soo Kim, Young-Sam Kwon, Sungho Yun
Summary: Wharton's jelly is a known source of mesenchymal stem cells in various species, but there have been no reports on cats. This study isolated and characterized mesenchymal stem cells from the Wharton's jelly of cats, demonstrating their differentiation potential and therapeutic applications in feline medicine.
VETERINARY SCIENCES
(2021)
Review
Chemistry, Medicinal
Benjamin J. Main, Nicola Maffulli, Josiah A. Valk, Hugo C. Rodriguez, Manu Gupta, Saadiq F. El-Amin, Ashim Gupta
Summary: Wharton's jelly-derived mesenchymal stem cells (WJSCs) have shown potential therapeutic benefits in orthopedic surgery, but further randomized controlled clinical studies are needed to assess their safety and efficacy.
Article
Biochemistry & Molecular Biology
Kuo-An Chu, Chang-Ching Yeh, Chun-Hsiang Hsu, Chien-Wei Hsu, Fu-Hsien Kuo, Pei-Jiun Tsai, Yu-Show Fu
Summary: This study compared the effectiveness of human umbilical cord Wharton's jelly-derived mesenchymal stem cells (HUMSCs) versus adipose tissue-derived mesenchymal stem cells (ADMSCs) for reversing pulmonary fibrosis in rats. The results showed that HUMSCs had better effects on lung function improvement and collagen degradation, as well as alveolar regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Beilei Ma, Tengkai Wang, Juan Li, Qian Wang
Summary: This study found that ECM derived from Wharton's Jelly-derived mesenchymal stem cells could improve the angiogenesis ability of HUVECs in vitro in a time-dependent manner. Mechanically, WJ-MSCs ECM activated the FAK/P38 signaling pathway via integrin alpha V beta 3 to promote c-Myc expression, subsequently increasing VEGF expression through P300-mediated histone acetylation.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Engineering, Biomedical
Shuangpeng Jiang, Guangzhao Tian, Zhen Yang, Xiang Gao, Fuxin Wang, Juntan Li, Zhuang Tian, Bo Huang, Fu Wei, Xinyu Sang, Liuqi Shao, Jian Zhou, Zhenyong Wang, Shuyun Liu, Xiang Sui, Quanyi Guo, Weimin Guo, Xu Li
Summary: This study confirms that hWJMSC-Exos can enhance the effect of the ACECM scaffold and promote osteochondral regeneration. The promoting effect may be related to the polarization of macrophages and inhibition of inflammatory response by hWJMSC-Exos. Additionally, hWJMSC-Exos contain miRNAs that can promote hyaline cartilage regeneration.
BIOACTIVE MATERIALS
(2021)
Article
Biotechnology & Applied Microbiology
Erwin Pavel Lamparelli, Maria Camilla Ciardulli, Valentina Giudice, Pasqualina Scala, Rosa Vitolo, Tina Patricia Dale, Carmine Selleri, Nicholas Robert Forsyth, Nicola Maffulli, Giovanna Della Porta
Summary: This study investigated the chondrogenic potentials of 3D high-density cultures of bone marrow and Wharton's Jelly-derived mesenchymal stromal cells (MSCs) supplemented with hTGF-beta 1. The study found that high-density cultures promoted chondrogenesis and that Wharton's Jelly-derived MSCs showed potential for chondrogenic commitment. These findings have important implications for cartilage regeneration and tissue engineering.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Monika Sypecka, Aleksandra Bzinkowska, Dorota Sulejczak, Filip Dabrowski, Anna Sarnowska
Summary: Wharton's jelly (WJ) from the umbilical cord (UC) is a good source of mesenchymal stem/stromal cells (MSCs) for therapy. Protocols for UC transportation, cell isolation, cultivation, and banking should be unified and optimized. WJ-MSCs showed higher levels of cytokines and chemokines secretion compared to WJ bioptats, and exhibited neural markers when exposed to cerebrospinal fluid (CSF). A protocol for manufacturing an advanced-therapy medicinal product was developed based on the findings.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Marta Dymowska, Aleksandra Aksamit, Katarzyna Zielniok, Monika Kniotek, Beata Kaleta, Aleksander Roszczyk, Michal Zych, Filip Dabrowski, Leszek Paczek, Anna Burdzinska
Summary: Although both BM-MSCs and WJ-MSCs have the ability to interact reciprocally with macrophages, the source of MSCs could slightly but significantly modify the response under clinical settings. WJ-MSCs secreted significantly more IL-6 than BM-MSCs and showed higher responsiveness to M1-derived chemotactic signals.
Article
Biochemistry & Molecular Biology
Katarzyna Stefanska, Lucie Nemcova, Malgorzata Blatkiewicz, Wojciech Pienkowski, Marcin Rucinski, Maciej Zabel, Paul Mozdziak, Marzenna Podhorska-Okolow, Piotr Dziegiel, Bartosz Kempisty
Summary: Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) possess multilineage differentiation potential and specific surface markers. In this study, RNA sequencing analysis revealed differentially expressed genes associated with apoptosis and identified upregulation of ZBTB16 and FOXO1, as well as downregulation of TGFA, in all differentiated groups. Additionally, several novel marker genes related to WJ-MSCs differentiation were identified. This study sheds light on the molecular mechanisms involved in long-term culture and differentiation of WJ-MSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Biomaterials
Xiufang Li, Renchuan You, Qiang Zhang, Shuqin Yan, Zuwei Luo, Jing Qu, Mingzhong Li
Summary: The research aims to improve cell transplantation efficacy by utilizing scaffolds with nanofibrous microstructure for cell colonization and vascularization. Experimental results demonstrate that multiscale hierarchical design significantly enhances the ability of transplanted cells to promote neovascularization and tissue reconstruction.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Biochemistry & Molecular Biology
Hong Thi Nguyen, Kasem Theerakittayakorn, Sirilak Somredngan, Apichart Ngernsoungnern, Piyada Ngernsoungnern, Pishyaporn Sritangos, Mariena Ketudat-Cairns, Sumeth Imsoonthornruksa, Juthaporn Assawachananont, Nattawut Keeratibharat, Rangsirat Wongsan, Ruttachuk Rungsiwiwut, Chuti Laowtammathron, Nguyen Xuan Bui, Rangsun Parnpai
Summary: This study reveals that WJ-MSCs have the potential to differentiate into CECs, which would be beneficial for further applications in LSCD treatment therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Polymer Science
Perng Yang Puah, Pak Yan Moh, Coswald Stephen Sipaut, Ping Chin Lee, Siew Eng How
Summary: Peptide-conjugated multilayer graphene oxide film was successfully fabricated and used for culturing human Wharton's jelly-derived mesenchymal stem cells. It was found that the peptide conjugate on the film supported the growth of WJ-MSCs and enhanced their osteogenic differentiation compared to the parent m-GO film, indicating its potential in bone tissue regeneration.
Article
Cell & Tissue Engineering
Emin Ozmert, Umut Arslan
Summary: The combination of WJ-MSC and high frequency rEMS therapy showed significant improvement in visual acuity and optic nerve structure in patients with toxic optic neuropathies. This combination therapy may offer a promising approach for currently untreatable cases, potentially preventing permanent blindness.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Environmental Sciences
Pei-Ying Lee, Bruce Chi-Kang Tsai, Maria Angelina Sitorus, Pi-Yu Lin, Shinn-Zong Lin, Cheng-Yen Shih, Shang-Yeh Lu, Yueh-Min Lin, Tsung-Jung Ho, Chih-Yang Huang
Summary: This study found that Ohwia caudata aqueous extract can effectively suppress doxorubicin-induced mitochondrial dysfunction, maintain the stability of stem cells, and reduce apoptosis rate. The findings provide new directions for the medical application of Ohwia caudata.
ENVIRONMENTAL TOXICOLOGY
(2023)
Article
Developmental Biology
Won-Jae Lee, Si-Jung Jang, Seung-Chan Lee, Ji-Sung Park, Ryoung-Hoon Jeon, Raghavendra Baregundi Subbarao, Dinesh Bharti, Jeong-Kyu Shin, Bong-Wook Park, Gyu-Jin Rho
REPRODUCTION FERTILITY AND DEVELOPMENT
(2017)
Article
Medicine, General & Internal
Iel-Yong Sung, Han-Na Son, Imran Ullah, Dinesh Bharti, Ju-Mi Park, Yeong-Cheol Cho, June-Ho Byun, Young-Hoon Kang, Su-Jin Sung, Jong-Woo Kim, Gyu-Jin Rho, Bong-Wook Park
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2016)
Article
Medicine, General & Internal
Young-Jin Han, Young-Hoon Kang, Sarath Belame Shivakumar, Dinesh Bharti, Young-Bum Son, Yong-Ho Cho, Won-Uk Park, June-Ho Byun, Gyu-Jin Rho, Bong-Wook Park
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2017)
Article
Multidisciplinary Sciences
Imran Ullah, Yong-ho Choe, Mehtab Khan, Dinesh Bharti, Sharath Belame Shivakumar, Hyeon-Jeong Lee, Young-Bum Son, Yurianna Shin, Sung-Lim Lee, Bong-Wook Park, Sun-A Ock, Gyu-Jin Rho
SCIENTIFIC REPORTS
(2018)
Article
Cell Biology
Sharath Belame Shivakumar, Dinesh Bharti, Raghavendra Baregundi Subbarao, Ju-Mi Park, Young-Bum Son, Imran Ullah, Yong-Ho Choe, Hyeong-Jeong Lee, Bong-Wook Park, Sung-Lim Lee, Gyu-Jin Rho
JOURNAL OF CELLULAR PHYSIOLOGY
(2019)
Article
Cell Biology
Dinesh Bharti, Sharath Belame Shivakumar, Young-Bum Son, Young-Ho Choi, Imran Ullah, Hyeon-Jeong Lee, Eun-Jin Kim, Sun-A Ock, Ji-Eun Park, Ji-Kwon Park, Dawon Kang, Sung-Lim Lee, Bong-Wook Park, Gyu-Jin Rho
CELL AND TISSUE RESEARCH
(2019)
Article
Biotechnology & Applied Microbiology
Ryoung-Hoon Jeon, Won-Jae Lee, Young-Bum Son, Dinesh Bharti, Sharath Belame Shivakumar, Sung-Lim Lee, Gyu-Jin Rho
BIOMED RESEARCH INTERNATIONAL
(2019)
Article
Cell Biology
Young-Noon Kang, Sharath Belame Shivakumar, Young-Bum Son, Dinesh Bharti, Si-Jung Jang, Kang-Sun Heo, Won-Uk Park, June-Ho Byun, Bong-Wook Park, Gyu-Jin Rho
ANIMAL CELLS AND SYSTEMS
(2019)
Article
Cell Biology
Won-Cheol Lee, Dae-Young Kim, Mi-Jeong Kim, Hyeon-Jeong Lee, Dinesh Bharti, Sung-Ho Lee, Young-Noon Kang, Gyu-Jin Rho, Byeong-Gyun Jeon
ANIMAL CELLS AND SYSTEMS
(2019)
Review
Cell Biology
Dinesh Bharti, Si-Jung Jang, Sang-Yun Lee, Sung-Lim Lee, Gyu-Jin Rho
Correction
Cell Biology
Dinesh Bharti, Si-Jung Jang, Sang-Yun Lee, Sung-Lim Lee, Gyu-Jin Rho
Article
Biotechnology & Applied Microbiology
Jinhee Ha, Dinesh Bharti, Young-Hoon Kang, Sang-Yeob Lee, Seong-Ju Oh, Saet-Byul Kim, Chan-Hee Jo, Jang-Ho Son, Iel-Yong Sung, Yeong-Cheol Cho, Gyu-Jin Rho, Jeong-Kil Park
Summary: This study demonstrated that dental pulp-derived mesenchymal stem cells could differentiate into smooth muscle-like cells under the influence of cytokines, with gelatin coating enhancing differentiation potential. However, the cells still showed immature characteristics, indicating the need for further research on different cytokine combinations and coating substrates to achieve fully functional mature smooth muscle-like cells.
BIOMED RESEARCH INTERNATIONAL
(2021)
Review
Biochemistry & Molecular Biology
Dinesh Bharti, Manisha Tikka, Sang-Yun Lee, Eun-Yeong Bok, Hyeon-Jeong Lee, Gyu-Jin Rho
Summary: Infertility is a major issue in modern society that can be caused by various factors. Understanding the basic concepts of germ cell development is crucial for improving our understanding of gametogenesis and finding treatments for related disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Young-Bum Son, Dinesh Bharti, Saet-Byul Kim, Chan-Hee Jo, Eun-Yeong Bok, Sung-Lim Lee, Young-Hoon Kang, Gyu-Jin Rho
Summary: The study compared the characteristics of 3D DPSC spheroids and 2D cultured DPSCs, showing that 3D culture enhances stemness and differentiation potential but may lead to decreased proliferative capacity, cell cycle arrest, and elevated apoptosis rate over time. Additionally, the study found that oxygen consumption rates of living cells decreased with the time course of the aggregation except for the initial 24 hours.
BIOMED RESEARCH INTERNATIONAL
(2021)
Article
Cell Biology
Dinesh Bharti, Sharath Belame Shivakumar, Ji-Kwon Park, Imran Ullah, Raghavendra Baregundi Subbarao, Ji-Sung Park, Sung-Lim Lee, Bong-Wook Park, Gyu-Jin Rho
CELL AND TISSUE RESEARCH
(2018)