4.7 Article

Wastewater used for urban agriculture in West Africa as a reservoir for antibacterial resistance dissemination

期刊

ENVIRONMENTAL RESEARCH
卷 168, 期 -, 页码 14-24

出版社

ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.envres.2018.09.022

关键词

Antimicrobial resistance; Metagenomics; Urban agriculture; Low and middle-income countries

资金

  1. European Union's Horizon 2020 Research and Innovation Programme under the Marie Sklodowska-Curie Grant [655398]
  2. Marie Curie Actions (MSCA) [655398] Funding Source: Marie Curie Actions (MSCA)
  3. MRC [MR/L015080/1] Funding Source: UKRI

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State of art metagenomics were used to investigate the microbial population, antibiotic resistance genes and plasmids of medical interest in wastewater used for urban agriculture in Ouagadougou (Burkina Faso). Wastewater samples were collected from three canals near agricultural fields in three neighbourhoods. Assessment of microbial population diversity revealed different microbial patterns among the different samples. Sequencing reads from the wastewaters revealed different functional specializations of microbial communities, with the predominance of carbohydrates and proteins metabolism functions. Eleven pathogen-specific and 56 orthologous virulence factor genes were detected in the wastewater samples. These virulence factors are usually found in human pathogens that cause gastroenteritis and/or diarrhoea. A wide range of antibiotic resistance genes was identified; 81 are transmissible by mobile genetic elements. These included seven different extended spectrum beta-lactamase genes encoding synthesis of four enzyme families, including two metallo-beta-lactamases (bla(AIM-1) and bla(GES-21)). Ten different incompatibility groups of Enterobacteriaceae plasmid replicons (ColE, FIB, FIC, FIL P, Q, R, U, Y, and A/C), and 30 plasmid replicon types from Gram-positive bacteria. All are implicated in the wide distribution of antibiotic resistance genes. We conclude that wastewater used for urban agriculture in the city represents a high risk for spreading bacteria and antimicrobial resistance among humans and animals.

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