期刊
DEVELOPMENT
卷 146, 期 2, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.159343
关键词
Wnt; Morphogen; Spinal cord; Roof plate; Neural stem cell; Regeneration; Mouse
资金
- Japan Society for the Promotion of Science [JP23370094, JP18H02454, JP24111002, JP17H05782, JP17K19418]
- Daiko Foundation grant of academic research on natural science [9141]
Cell morphology changes dynamically during embryogenesis, and these changes create new interactions with surrounding cells, some of which are presumably mediated by intercellular signaling. However, the effects of morphological changes on intercellular signaling remain to be fully elucidated. In this study, we examined the effect of morphological changes in Wnt-producing cells on intercellular signaling in the spinal cord. After mid-gestation, roof-plate cells stretched along the dorsoventral axis in the mouse spinal cord, resulting in new contact at their tips with the ependymal cells that surround the central canal. Wnt1 and Wnt3a were produced by the stretched roof-plate cells and delivered to the cell process tip. Whereas Wnt signaling was activated in developing ependymal cells, Wnt activation in dorsal ependymal cells, which were close to the stretched roof plate, was significantly suppressed in embryos with roof plate-specific conditional knockout of Wls, which encodes a factor that is essential for Wnt secretion. Furthermore, proliferation of these cells was impaired in Wls conditional knockout mice during development and after induced spinal cord injury in adults. Therefore, morphological changes in Wnt-producing cells appear to generate new Wnt signal targets.
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