Characterization of uronate dehydrogenases catalysing the initial step in an oxidative pathway

Uronate dehydrogenases catalyse the oxidation of uronic acids to aldaric acids, which represent top value-added chemicals' that have the potential to substitute petroleum-derived chemicals. The identification and annotation of three uronate dehydrogenases derived from Fulvimarina pelagiHTCC2506, Streptomyces viridochromogenesDSM 40736 and Oceanicola granulosusDSM 15982 via sequence analysis is described. Characterization and comparison with two known uronate dehydrogenases in regard to substrate spectrum, catalytic activity and pH as well as temperature dependence was performed. The catalytic efficiency was investigated in two different buffer systems; potassium phosphate and Tris-HCl. In addition to the typical and well available substrates glucuronate and galacturonate also mannuronate as part of many structural polysaccharides were tested. The uronate dehydrogenase of Agrobacterium tumefaciens and Pseudomonas syringae showed catalytic dependency on the buffer system resulting in an increased K-m especially for glucuronate in potassium phosphate compared with Tris-HCl buffer. Enzyme stability at 37 degrees C of the different Udhs was in the order: P.syringae