期刊
CELL HOST & MICROBE
卷 25, 期 1, 页码 140-+出版社
CELL PRESS
DOI: 10.1016/j.chom.2018.11.008
关键词
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资金
- Swiss National Science Foundation (Switzerland) [CRSII3_127433, 310030B_147090]
- Gebert Ruf Foundation (Germany) [GRS-035/12]
- Swiss National Science Foundation (SNF) [CRSII3_127433] Funding Source: Swiss National Science Foundation (SNF)
The opportunistic human pathogen Pseudomonas aeruginosa effectively colonizes host epithelia using pili as primary adhesins. Here we uncover a surface-specific asymmetric virulence program that enhances P. aeruginosa host colonization. We show that when P. aeruginosa encounters surfaces, the concentration of the second messenger c-di-GMP increases within a few seconds. This leads to surface adherence and virulence induction by stimulating pili assembly through activation of the c-di-GMP receptor FimW. Surface-attached bacteria divide asymmetrically to generate a piliated, surface-committed progeny (striker) and a flagellated, motile offspring that leaves the surface to colonize distant sites (spreader). Cell differentiation is driven by a phosphodiesterase that asymmetrically positions to the flagellated pole, thereby maintaining c-di-GMP levels low in the motile offspring. Infection experiments demonstrate that cellular asymmetry strongly boosts infection spread and tissue damage. Thus, P. aeruginosa promotes surface colonization and infection transmission through a cooperative virulence program that we termed Touch-Seed-and-Go.
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