期刊
CARBOHYDRATE POLYMERS
卷 203, 期 -, 页码 119-127出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2018.09.045
关键词
Ginseng; Pectin; Rhamnogalacturonan-I; Rhamnogalacturonan-II
资金
- National Natural Science Foundation of China [31770852, 31470798]
- Scientific and Technologic Foundation of Jilin Province [20180101004JC]
Rhamnogalacturonan I (RG-I) and rhamnogalacturonan II (RG-II) domains were isolated from ginseng pectin by alkali saponification and endo-polygalacturonase hydrolysis, then purified by anion-exchange and size-exclusion chromatography. Monoclonal antibody detection indicated that ginseng RG-I contained -> 4)-alpha-GalpA-(1 -> 2)-alpha-Rhap-(1 -> repeating units as backbone, with arabinan, galactan and type II arabinogalactan (AG-II) as side chains. The use of galactose- and arabinose-releasing enzymes, mass spectrometry analysis of the oligosaccharides generated by rhamnogalacturonan hydrolase, and glycosidic linkage analyses provided evidence that ginseng RG-I contains both single galactose-branched subunits and highly branched subunits with arabinan and AG-II side chains. RG-II was analyzed by sequential acid hydrolysis followed by mass spectrometry. Ginseng RG-II contains 9 galacturonic acid units as backbone. Side chain A is an octasaccharide with 0 similar to 1 methyl ether group. Side chain B is a nonasaccharide with 0 similar to 1 acetyl group. These results provide useful information for further investigation of structure-activity relationship of ginseng pectin.
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