Article
Cell & Tissue Engineering
Naoki Ishiuchi, Ayumu Nakashima, Satoshi Maeda, Yoshie Miura, Kisho Miyasako, Kensuke Sasaki, Toshio Uchiki, Ayano Sasaki, Shogo Nagamatsu, Naoki Nakao, Masataka Nagao, Takao Masaki
Summary: Fibrosis is a common histological feature in chronic organ injury, leading to organ failure. Adipose-derived mesenchymal stem cells (ASCs) have been studied as a therapeutic tool to prevent organ failure. This study compared the therapeutic effects of ASCs from superficial and deep layers and found that both types of ASCs are equally effective and safe for treating organ injury.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Cell Biology
Furkan Yigitbilek, Sabena M. Conley, Hui Tang, Ishran M. Saadiq, Kyra L. Jordan, Lilach O. Lerman, Timucin Taner
Summary: MSCs from liver and adipose tissue showed similar proliferation and migration properties, but had differences in immunomodulatory and pro-angiogenic abilities. Selecting cell-based therapy according to the specific characteristics of different cell sources may be important for treating certain diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jun Ho Lee, Yu Jin Won, Hail Kim, Minji Choi, Esther Lee, Bumsik Ryoou, Seok-Geun Lee, Byong Seung Cho
Summary: Exosomes derived from adipose tissue-derived stem cells (ASCs) have shown beneficial effects on cell proliferation, migration, and gene expression related to wound healing. Combining these ASC-derived exosomes (ASC-EXOs) with hyaluronic acid (HA) can enhance wound closure rates, tissue regeneration, and collagen production in animal models. This suggests the potential for their use in clinical and regenerative aesthetic applications for skin repair and regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Ahmed Abdelbaset-Ismail, Abeer Tharwat, Ahmed E. Ahmed, Tarek Khamis, Ibrahim H. Abd El-Rahim, Sadeq K. Alhag, Mohamed F. Dowidar
Summary: This study found that rat adipose-derived mesenchymal stem cells (rAD-MSCs) could alleviate diclofenac sodium (DIC)-induced acute liver injury by being transplanted into the injured liver. This treatment method significantly improved survival rate, body weight, and liver function indicators, while reducing inflammation and cell damage.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Biochemistry & Molecular Biology
Maria Yoshida, Ayumu Nakashima, Naoki Ishiuchi, Kisho Miyasako, Keisuke Morimoto, Yoshiki Tanaka, Kensuke Sasaki, Satoshi Maeda, Takao Masaki
Summary: Both adipose-derived and bone marrow-derived mesenchymal stem cells (ADSCs and BMSCs) can suppress renal fibrosis, with ADSCs showing a stronger effect under serum-containing culture conditions. However, ADSC infusion in mice may lead to a higher death rate from pulmonary embolism. Therefore, the selection of ADSCs or BMSCs for clinical applications should consider the target disease and culture method.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Medicine, General & Internal
Robert Ossendorff, Alessandra Menon, Frank A. Schildberg, Pietro S. Randelli, Sebastian Scheidt, Christof Burger, Dieter C. Wirtz, Davide Cucchi
Summary: The global distribution and quality of studies using adipose tissue derivates in orthopedics were assessed through a systematic review. Among the 82 included studies, 70% focused on knee disorders, mainly osteoarthritis. Approximately 26% of the studies investigated expanded adipose-derived stem/stromal cells (ADSCs), with 72% of these using stromal vascular fraction (SVF). The overall quality of the studies varied, with a higher score found in studies involving expanded ADSCs compared to SVF.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Orthopedics
Xing Guo, Denghua Huang, Dan Li, Longfei Zou, Hui Lv, Yuanhui Wang, Meiyun Tan
Summary: This study found that hypoxia preconditioning attenuates the proliferation and migration ability of ADSCs, but has positive impact on tenogenic differentiation through HIF-1 alpha signaling pathway.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2022)
Article
Agriculture, Dairy & Animal Science
Byong Seung Cho, Sung-Bae Kim, Sokho Kim, Beomseok Rhee, Jungho Yoon, Jae Won Lee
Summary: In this study, the effects of canine ASC-exosomes on canine AD were investigated using a Biostir-induced AD mouse model. The study found that cASC-EVs improved AD-like dermatitis and decreased serum IgE, ear thickness, and inflammatory cytokines such as IL-4 and IFN-γ in a dose-dependent manner. In vivo toxicity studies showed no systemic toxicity. miRNA analysis showed the presence of anti-inflammatory miRNAs in cASC-EVs, suggesting a promising cell-free therapy for treating canine AD.
Review
Hematology
Yukako Ono-Uruga, Yasuo Ikeda, Yumiko Matsubara
Summary: The review focuses on the application of ex vivo technologies in MK development using human adipose tissue-derived mesenchymal stem/stromal cell line (ASCL), aiming to address the clinical shortage of platelets. Manufacturing platelets from adipose tissue-derived mesenchymal stem/stromal cells (ASCs) without the need for donors may lead to sufficient platelet production within 12 days.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Biochemistry & Molecular Biology
Siti Syahira Airuddin, Ahmad Sukari Halim, Wan Azman Wan Sulaiman, Ramlah Kadir, Nur Azida Mohd Nasir
Summary: Stem cells, particularly adipose-derived stem cells, have shown promising potential in disease treatment due to their differentiation abilities and secretion of growth factors. However, more research is needed to fully understand the safety and effectiveness of adipose-derived stem cells in medical treatment.
Article
Cell Biology
Assia Eljaafari, Julien Pestel, Brigitte Le Magueresse-Battistoni, Stephanie Chanon, Julia Watson, Maud Robert, Emmanuel Disse, Hubert Vidal
Summary: The study revealed that PD-L1 is overexpressed in the adipose tissue of obese individuals, leading to T cell dysfunction and notably reduced cytolytic activity. PD-L1 plays a role in T cell dysfunction mediated by obese ASC.
Review
Cell Biology
Yuan Liu, Christina Holmes
Summary: This review explores the tissue regeneration potential of EVs isolated from bone marrow and adipose tissue, the two most common clinical sources of adult MSCs, focusing on their angiogenic, osteogenic, and immunomodulatory potentials. Other important issues in the development of MSC-derived EV based therapies are also discussed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Immunology
Yosuke Niimi, Tuvshintugs Baljinnyam, Satoshi Fukuda, Clark R. Andersen, John R. Salsbury, Jong O. Lee, Donald S. Prough, Perenlei Enkhbaatar
Summary: This study aimed to test the efficacy of nebulized adipose-derived mesenchymal stem cells (ASCs) in a well-characterized ovine model of smoke inhalation injury. The results showed that the PaO2/FiO2 in the ASCs group was significantly higher and the oxygenation index was significantly lower compared to the control group. The viability of ASCs in bronchoalveolar lavage fluid was lower than those exposed to the control solution. Nebulized ASCs moderately improved pulmonary function and delayed the onset of ARDS.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Manuela Heimann, Mohamed I. Elashry, Michele C. Klymiuk, Asmaa Eldaey, Sabine Wenisch, Stefan Arnhold
Summary: The investigation of mesenchymal stem cells has become an interesting scientific field in regenerative medicine and organ transplantation. This study focused on establishing an adipogenic differentiation protocol suitable for different mammalian species, and found that using rosiglitazone instead of traditional protocols significantly improves cell viability and adipogenic differentiation capacity. The study also revealed differences between species in terms of fat formation and fat-regulating gene expression. This information is essential for selecting a stem cell specification program and improving clinical targeting of obesity and metabolic disorders in veterinary medicine.
Review
Biochemistry & Molecular Biology
Parviz Vahedi, Rana Moghaddamshahabi, Thomas J. Webster, Ayse Ceren Calikoglu Koyuncu, Elham Ahmadian, Wasim S. Khan, Ali Jimale Mohamed, Aziz Eftekhari
Summary: Cartilage damage is often difficult to repair, and current treatment strategies are inadequate. Autologous mesenchymal stem cells are considered a promising therapeutic approach for cartilage regeneration, as they possess robust cartilaginous production capacity and are easy to isolate and expand without causing morbidity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Emergency Medicine
Pil Young Jung, Jae Sik Chung, Youngin Youn, Chang Wan Kim, Il Hwan Park, Oh Hyun Kim, Chun Sung Byun
Summary: Pediatric thoracic trauma differs from adults due to small anatomy and rapid recovery. Data from 168 patients under 15 years old with thoracic trauma were analyzed, with pedestrian traffic accidents being the most common cause of injury and lung contusion being the most common injury. Independent risk factors of in-hospital mortality were hemopneumothorax and cardiac contusion.
EUROPEAN JOURNAL OF TRAUMA AND EMERGENCY SURGERY
(2022)
Article
Cell Biology
Seongyup Kim, Pil Young Jung, Jin Suk Lee, Soonjae Hwang, Joon Hyung Sohn, Yongdae Yoon, Keum Seok Bae, Young Woo Eom
Summary: The study revealed that SkMSCs lose their satellite cell properties during serial passaging but acquire mesenchymal stem cell properties, including the ability to exert an anti-inflammatory response for macrophages through PGE2 and HGF.
CELL BIOLOGY INTERNATIONAL
(2021)
Article
Medicine, General & Internal
Jae Sik Chung, Soonjae Hwang, Ju Eun Hong, Minjeong Jo, Ki-Jong Rhee, Seongyup Kim, Pil Young Jung, Youngdae Yoon, Seong Hee Kang, Hoon Ryu, Moon Young Kim, Keum Seok Bae, Young Woo Eom
Summary: Cultured human skeletal-muscle satellite cells (SkMSCs) have shown anti-inflammatory properties by secreting prostaglandin E2 and hepatocyte growth factor (HGF). In this study, it was found that a single treatment with SkMSCs could ameliorate acute liver and gut inflammation induced by binge ethanol administration in mice, reducing markers of liver injury, bacterial-derived lipopolysaccharide levels, tissue inflammation, and leaky gut indicators.
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Chang-Gun Lee, Chanoh Park, Soonjae Hwang, Ju-Eun Hong, Minjeong Jo, Minseob Eom, Yongheum Lee, Ki-Jong Rhee
Summary: This study examined the effect of pulsed electromagnetic field (PEMF) therapy on a mouse model of lipopolysaccharide (LPS)-induced septic shock. The results showed that PEMF treatment increased the survival rate of mice and decreased pro-inflammatory cytokine gene expression and liver damage. Microarray analysis identified several genes regulated by PEMF therapy. Therefore, PEMF may be beneficial for patients with bacteria-induced sepsis and reduce septic shock-induced mortality.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Chang-Gun Lee, Soonjae Hwang, Sun-Yeong Gwon, Chanoh Park, Minjeong Jo, Ju-Eun Hong, Ki-Jong Rhee
Summary: Researchers have found that the secretion of BFT by ETBF can induce IL-8 secretion in intestinal epithelial cells by activating the E-cadherin and beta-catenin signaling pathways, leading to acute inflammation.
Article
Biochemistry & Molecular Biology
Seong Chan Gong, Yongdae Yoon, Pil Young Jung, Moon Young Kim, Soon Koo Baik, Hoon Ryu, Young Woo Eom
Summary: This study investigated the expression of TSG-6 in mesenchymal stem cells and found that it is influenced by the polarization state of macrophages. Furthermore, it was discovered that TSG-6 expression is increased through STAT1/3 activation in an inflammatory microenvironment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Ju-Eun Hong, Chang-Gun Lee, Soonjae Hwang, Junyoung Kim, Minjeong Jo, Da-Hye Kang, Sang-Hyeon Yoo, Woo-Seung Kim, Yongheum Lee, Ki-Jong Rhee
Summary: This study found that pulsed electromagnetic field (PEMF) can alleviate inflammation in rheumatoid arthritis (RA), particularly at a frequency of 10 Hz. The treated mice had lower levels of joint inflammation and IL-1 beta. The results suggest that PEMF treatment can preserve joint morphology and delay the occurrence of RA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Chang Wan Kim, Yongdae Yoon, Moon Young Kim, Soon Koo Baik, Hoon Ryu, Il Hwan Park, Young Woo Eom
Summary: This study investigated the antifibrotic effect of protein kinase C (PKC) in myofibroblasts and its involvement in Hippo pathway activation. The results show that PKC delta exerts an antifibrotic effect by inhibiting the Hippo pathway in hepatic stellate cells (HSCs). PKC delta and YAP can be potential therapeutic targets for fibrotic diseases.
Article
Biochemistry & Molecular Biology
Rokeya Akter, Kwangmin Kim, Hye Youn Kwon, Youngwan Kim, Young Woo Eom, Hye-mi Cho, Mee-Yon Cho
Summary: EMR1 expression in tumor cells is significantly associated with lymph node metastasis and tumor-associated macrophages (CD68 and CD163) in colorectal cancer. The expression of EMR1 in cancer cells may be related to M2 macrophage polarization.
Article
Medicine, General & Internal
Jae Sik Chung, Sanghyun An, Seong Chan Gong, Pil Young Jung
Summary: This study investigated the usefulness of whole-body bone scans (WBBS) in detecting missed skeletal injuries in patients with multiple traumas. The results showed that factors such as high injury severity score, admission route through surgery and embolization, orthopedic surgery involvement, and shock were associated with missed skeletal injuries.
Article
Health Care Sciences & Services
Sung Woo Jang, Hae Rim Kim, Pil Young Jung, Jae Sik Chung
Summary: In this study, various environmental factors including age, time of injury, and precipitation were found to be significant predictors of mortality in patients with severe trauma.
Article
Biology
Sun Young Lee, Eun-Ok Kim, Daehyuk Jang, Soonjae Hwang, Ki-Jong Rhee, Miyong Yun
Summary: Research on the effective attachment of aptamers to beads has made little progress. This study demonstrates a new method using flow cytometry to determine the optimal aptamer-to-bead ratio for immobilization. The proposed method shows increased fluorescence intensity with increasing aptamer concentration, peaking at a ratio of 2.56 x 10(5), and decreasing at higher ratios. Analysis using fluorescence microscopy yielded similar results. However, there was a significant loss of aptamer compared to a ratio of 1.28 x 10(5) after the conjugation reaction, and the binding efficiency was highest at the ratio of 1.28 x 10(5). These findings suggest that the proposed method is the best and easiest for determining the optimal aptamer-to-bead ratio.
Article
Oncology
Young Un Choi, Yongdae Yoon, Pil Young Jung, Soonjae Hwang, Ju Eun Hong, Woo-Seung Kim, Joon Hyung Sohn, Ki-Jong Rhee, Keum Seok Bae, Young Woo Eom
Summary: The study found that TRAIL-overexpressing ASCs can be used to treat tumors, with ASC-IFN-beta secreting a higher level of TRAIL and showing enhanced therapeutic effects on H460 tumors.
CANCER GENOMICS & PROTEOMICS
(2021)
Review
Oncology
Xinru Zhou, Yin Jia, Chuanbin Mao, Shanrong Liu
Summary: Small extracellular vesicles (sEVs), such as exosomes, have emerged as crucial targets for liquid biopsy and promising drug delivery vehicles in tumor progression. They can serve as biomarkers for tumor diagnosis and as drug carriers for cancer treatment.
Article
Oncology
Ruochan Chen, Ju Zhu, Xiao Zhong, Jie Li, Rui Kang, Daolin Tang
Summary: The interplay between autophagy and apoptosis plays a crucial role in tumorigenesis and cancer therapy, with HMGB1 serving as a key regulator in these processes.
Article
Oncology
Zongfu Pan, Xixuan Lu, Tong Xu, Jinming Chen, Lisha Bao, Ying Li, Yingying Gong, Yulu Che, Xiaozhou Zou, Zhuo Tan, Ping Huang, Minghua Ge
Summary: This study uncovered the emerging role of HN1 in promoting dedifferentiation of anaplastic thyroid cancer (ATC) cells. HN1 negatively regulated the thyroid differentiation markers and had an inhibitory effect on the transcriptional activation of CTCF, thereby influencing the chromatin accessibility of thyroid differentiation genes.
Article
Oncology
Yi Qin, Shengjun Xiong, Jun Ren, Gautam Sethi
Summary: Autophagy plays an important regulatory role in glioblastoma, and its dysregulation can lead to drug resistance and radioresistance. It also affects stem cell characteristics, overall growth, and metastasis. Therefore, autophagy is a promising target for glioblastoma therapy.
Article
Oncology
Katsuya Nagaoka, Xuewei Bai, Dan Liu, Kevin Cao, Joud Mulla, Chengcheng Ji, Hongze Chen, Muhammad Azhar Nisar, Amalia Bay, William Mueller, Grace Hildebrand, Jin-Song Gao, Shaolei Lu, Hiroko Setoyama, Yasuhito Tanaka, Jack R. Wands, Chiung-Kuei Huang
Summary: This study found that serum 2-OG levels in cholangiocarcinoma patients are associated with the effectiveness of chemotherapy. Patients with progressive disease showed significantly higher levels of serum 2-OG compared to stable disease and partial response patients. The study also revealed that overexpression of ASPH mimics the effects of 2-OG, and knockdown of ASPH improves chemotherapy. Targeting ASPH enhances the effects of chemotherapy by modulating ATM and ATR, two key regulators of DDRs.