Article
Cell Biology
Mei-Hua Jin, Nan-Nan Yu, Ying-Hua Jin, Ying-Ying Mao, Lin Feng, Yue Liu, Ai-Guo Wang, Hu-Nan Sun, Taeho Kwon, Ying-Hao Han
Summary: This study found that Prx II accelerates wound healing in DMSCs by protecting them from apoptosis induced by reactive oxygen species, without regulating cell/growth factor secretion. Additionally, Prx II potentially regulates exosome functions through miR-21-5p and miR-221.
Article
Cell Biology
Ying-Hao Han, Ying-Ying Mao, Kyung Ho Lee, Hee Jun Cho, Nan-Nan Yu, Xiao-Ya Xing, Ai-Guo Wang, Mei-Hua Jin, Kwan Soo Hong, Hu-Nan Sun, Taeho Kwon
Summary: Our study investigated the role of Prx II in regulating exosome secretion from DMSCs and elucidated the underlying molecular mechanisms. We found that depletion of Prx II significantly decreased exosome secretion from DMSCs and increased the number of intracellular MVBs. Mechanistically, Prx II regulates the ISGylation switch that controls MVB degradation and impairs exosome secretion. These findings provide important insights into the molecular mechanisms governing exosome secretion from DMSCs and highlight the critical role of Prx II in controlling this process.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Medicine, Research & Experimental
Zheng Zhang, Minghui Wang, Youli Zheng, Yanmei Dai, Jiashu Chou, Xiaowei Bian, Pengcheng Wang, Changyi Li, Jing Shen
Summary: This study found that miR-223 is upregulated in periodontitis and downregulated during the osteogenesis of PDL-derived cells. Overexpression of miR-223 inhibits osteogenic differentiation of PDL-derived cells, potentially by targeting the growth factor receptor genes FGFR2 and TGFβR2.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Shuang Zhao, Shangyun Lu, Lihong Fan, Hongbo Hu
Summary: Glycyrol has been shown to be effective against gefitinib-resistant non-small cell lung cancer and can increase the sensitivity of resistant cells to gefitinib, suggesting its potential as a novel agent for treating gefitinib-resistant NSCLC.
APPLIED SCIENCES-BASEL
(2021)
Article
Medicine, Research & Experimental
Zhihui Gao, Qianqing Wang, Mei Ji, Xiangcui Guo, Li Li, Xiaoke Su
Summary: The study revealed that UCA1 regulates SOX2 expression by sponging miR-122-5p, affecting the functions of CC stem cells, while CaSki-Exo promotes the invasion and migration of CC stem cells. Silencing UCA1 or up-regulating miR-122-5p reduced the invasion and migration abilities of CC stem cells.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Plant Sciences
Qiumei Fan, Xiaowei Liang, Zhipeng Xu, Siyuan Li, Shan Han, Yuntian Xiao, Qiongming Xu, Renyikun Yuan, Shilin Yang, Hongwei Gao
Summary: In this study, it was found that Pedunculoside (PE), a triterpene saponin extracted from Ilex rotunda Thunb., can reverse NSCLC metastasis and improve Gefitinib resistance through the MAPK and Nrf(2) pathways, subsequently suppressing lung metastasis in B16-F10 lung metastatic mice model. These findings suggest that PE has potential use in inhibiting NSCLC metastasis and improving Gefitinib resistance.
Article
Cell & Tissue Engineering
Cong Fan, Xiaohan Ma, Yuejun Wang, Longwei Lv, Yuan Zhu, Hao Liu, Yunsong Liu
Summary: The study confirmed that miR-137 directly targets the 3 ' UTR of NOTCH1 while positively regulates HES1 expression. Additionally, it was found that tangeretin can reverse the effects of miR-137 knockdown on osteogenic promotion and downstream gene expression. Furthermore, a co-regulatory signaling network of NOTCH1/LSD1/BMP2 was proposed to elucidate the modulation of miR-137 on the osteoblastic differentiation of hASCs.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Pharmacology & Pharmacy
Peiheng Cai, Gaofan Sheng, Shiqin Jiang, Daifei Wang, Zhongxiang Zhao, Min Huang, Jing Jin
Summary: This study revealed the molecular mechanism of how Cryptotanshinone (CTS) enhances the sensitivity of lung cancer H1975 cells to gefitinib using a proteomic approach. The results demonstrated that CTS mainly enhances the efficacy of gefitinib by impacting the redox process and fatty acid metabolism in H1975 cells.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Oncology
Junyong Zou, Huiyin Lan, Wei Li, Shuanshuan Xie, Zhongkai Tong, Xiaolian Song, Changhui Wang
Summary: This study provides valuable data on the expression profiles of circRNAs detected in liquid biopsy for LUAD patients with acquired gefitinib resistance. We validate that upregulations of hsa_circ_0030591 and hsa_circ_0040348 may play key roles in EGFR-TKI resistance and thus serving as candidates for biomarkers.
TECHNOLOGY IN CANCER RESEARCH & TREATMENT
(2022)
Article
Oncology
Takayuki Nakanishi, Yasuto Yoneshima, Koji Okamura, Toyoshi Yanagihara, Mikiko Hashisako, Takeshi Iwasaki, Naoki Haratake, Shun Mizusaki, Keiichi Ota, Eiji Iwama, Tomoyoshi Takenaka, Kentaro Tanaka, Tomoharu Yoshizumi, Yoshinao Oda, Isamu Okamoto
Summary: Treatment with immune checkpoint inhibitors in non-small-cell lung cancer patients leads to a durable response, but drug resistance eventually develops. CD155 upregulation is identified as a mechanism of resistance to PD-1/PD-L1 inhibitors, and understanding CD155 regulation is important. This study identified miR-346, miR-328-3p, miR-326, and miR-330-5p as miRNAs that bind to CD155 mRNA and suppress its expression. The abundance of miR-326 was significantly higher in lung adenocarcinoma specimens with low CD155 expression, suggesting it may play a role in resistance development.
Article
Medicine, Research & Experimental
Jie Li, Xiaoxia Zeng, Weiqing Wang
Summary: miR-122-5p can induce acute lung injury (ALI) by promoting cell apoptosis and increasing LDH release, while its role in ALI may be related to IL-1 receptor antagonist (IL1RN). The effects of miR-122-5p inhibitor on LPS-treated A549 cells were opposite to those of miR-122-5p mimic, and all these effects could be significantly reversed by IL1RN-siRNA.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Oncology
Jung Hee Cho, Yeon-Mi You, Han Koo, Dong Chul Lee, Young Il Yeom, Kyung Chan Park
Summary: This study identified LPIN1 as a key factor in regulating gefitinib resistance in EGFR-mutant NSCLC cells, and found that LPIN1 expression was induced following gefitinib treatment, leading to increased lipid droplet formation and activation of protein kinase C delta and nuclear factor kappa B. Targeting LPIN1 was shown to synergistically inhibit tumor growth, suggesting a potential effective strategy for preventing TKI resistance in NSCLC patients.
Article
Oncology
Fariz Nurwidya, Fumiyuki Takahashi, Wira Winardi, Ken Tajima, Yoichiro Mitsuishi, Akiko Murakami, Isao Kobayashi, Takeshi Nara, Muneaki Hashimoto, Motoyasu Kato, Moulid Hidayat, Kentaro Suina, Daisuke Hayakawa, Tetsuhiko Asao, Ryo Ko, Takehito Shukuya, Toshifumi Yae, Naoko Shimada, Yasuko Yoshioka, Shinichi Sasaki, Kazuhisa Takahashi
Summary: ZEB1 is crucial for gefitinib-resistant lung CSCs with EMT features by regulating miR-200c and BMI1. Silencing ZEB1 attenuates miR-200c suppression, leading to reduced BMI1 and reversal of mesenchymal and CSC characteristics in GRPs. Overexpression of ZEB1 induces EMT and increases CD133- and BMI1-positive GRPs in vitro, promoting gefitinib resistance in vivo.
Article
Oncology
Alexis B. Cortot, Anne Madroszyk, Etienne Giroux-Leprieur, Olivier Molinier, Elisabeth Quoix, Henri Berard, Josiane Otto, Isabelle Rault, Denis Moro-Sibilot, Judith Raimbourg, Elodie Amour, Franck Morin, Jose Hureaux, Lionel Moreau, Didier Debieuvre, Hugues Morel, Aldo Renault, Eric Pichon, Benjamin Huret, Sandrine Charpentier, Marc G. Denis, Jacques Cadranel
Summary: The addition of cetuximab to afatinib in the treatment of treatment-naive advanced EGFR-mutant NSCLC did not show any significant improvement in efficacy, suggesting that further investigation into this combination therapy may not be warranted.
CLINICAL CANCER RESEARCH
(2021)
Article
Chemistry, Physical
Ehab M. M. Elzayat, Abdelrahman Y. Y. Sherif, Fahd A. A. Nasr, Mohamed W. W. Attwa, Doaa H. H. Alshora, Sheikh E. F. Ahmad, Ali S. S. Alqahtani
Summary: Lung cancer poses a great challenge in clinical practice due to its metastatic nature and resistance to conventional treatments. The codelivery of anticancer agents within nanostructured lipid carriers (NLCs) offers a potential solution to overcome resistance and minimize systemic toxicity. In this study, a GEF-AZT-NLC formulation was developed and showed promise as a potential therapeutic tool for treating metastatic-resistant lung cancer.
Article
Environmental Sciences
Jiao Jiao Zhang, Ya Qi Li, Mei Shi, Cheng Chen Deng, Yu Sha Wang, Yao Tang, Xian Zhong Wang
Summary: Thiazolidinedione (TZD) inhibits the proliferation of immature chicken Sertoli cells (SCs) but this damage can be mitigated by up-regulating miR-1577 expression with the help of 178-estradiol.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Reproductive Biology
Lu Gan, Sha Huang, Yu Hu, JiaoJiao Zhang, XianZhong Wang
Summary: This study found that heat treatment can improve insulin sensitivity by enhancing the effect of insulin on lactate secretion and regulating the expression of miR-7-5p/IRS2/PI3K/Akt. These findings reveal a novel mechanism of heat treatment on the regulation of lactate metabolism and suggest that targeting miR-7-5p may be a potential therapeutic approach for metabolic diseases induced by insulin dysfunction.
Article
Food Science & Technology
Ying-Hao Han, Ying-Ying Mao, Yao-Yuan Feng, Hong-Yi Xiang, Hu-Nan Sun, Mei-Hua Jin, Taeho Kwon
Summary: This study identified the role of Prx II in DMSCs through RNA sequencing and Gene Ontology analysis, showing that Prx II plays a key role in cell adhesion and cell homing by regulating multiple genes. The findings provide a theoretical basis for improving the homing ability of DMSCs.
APPLIED BIOLOGICAL CHEMISTRY
(2022)
Article
Oncology
Ying-Hao Han, Yuan Wang, Seung-Jae Lee, Ying-Ying Mao, Peng Jiang, Hu-Nan Sun, Mei-Hua Jin, Taeho Kwon
Summary: The study found that mRNA-miRNA-lncRNA interactions may play a role in the development of TNBC, with cell adhesion playing an important role. Key genes and miRNAs related to the occurrence and development of TNBC were identified.
ANTICANCER RESEARCH
(2023)
Article
Veterinary Sciences
Taeho Kwon, Hong-Yi Xiang, Xiao-Ya Xing, Peng Jiang, Shuai-Yang Sun, Hu-Nan Sun, Ying-Hao Han
Summary: By analyzing SIV and SIVE encephalitis in infected M. mulatta hippocampus samples, we identified eight genes (MX1, B2M, IFIT1, TYMP, STAT1, IFI44, ISG15, and IFI27) that affect the negative regulation of biological processes, hepatitis C and Epstein-Barr viral infection, and the toll-like receptor signaling pathway, mediating the development of encephalitis after SIV infection. Particularly, STAT1 plays a central role in the process by regulating biopathological changes during the development of SIVE.
JOURNAL OF MEDICAL PRIMATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ying-Hao Han, Xin-Mei He, Mei-Hua Jin, Hu-Nan Sun, Taeho Kwon
Summary: Lipid droplets are specialized lipid storage organelles in hepatocytes, and lipophagy is a crucial mechanism for selectively degrading lipid droplets through lysosomes. It plays a critical role in the prevention and treatment of metabolic liver diseases such as NAFLD and AFLD. This review highlights recent research on key proteins and molecular mechanisms related to lipophagy in liver disease.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Oncology
Adam J. Sugarman, Luong Do Huynh, Aidin Shabro, Antonio Di Cristofano
Summary: Anaplastic thyroid cancer (ATC) is an aggressive and lethal tumor, characterized by loss of differentiation, high proliferation rate, and resistance to therapy. The upregulation of the one-carbon metabolic pathway in ATC cells, which generates nucleotides and glycine, was identified as a vulnerability that can be targeted for therapy. Inhibition of the key enzyme SHMT2 in this pathway led to glycine auxotrophy and significant inhibition of cell proliferation, especially in the presence of physiological levels of folates.
Article
Cell Biology
Ying-Hao Han, Ying-Ying Mao, Kyung Ho Lee, Hee Jun Cho, Nan-Nan Yu, Xiao-Ya Xing, Ai-Guo Wang, Mei-Hua Jin, Kwan Soo Hong, Hu-Nan Sun, Taeho Kwon
Summary: Our study investigated the role of Prx II in regulating exosome secretion from DMSCs and elucidated the underlying molecular mechanisms. We found that depletion of Prx II significantly decreased exosome secretion from DMSCs and increased the number of intracellular MVBs. Mechanistically, Prx II regulates the ISGylation switch that controls MVB degradation and impairs exosome secretion. These findings provide important insights into the molecular mechanisms governing exosome secretion from DMSCs and highlight the critical role of Prx II in controlling this process.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Environmental Sciences
Ze-Min Zhao, Su Mei, Qi-Yue Zheng, Jiao Wang, Yi-Ru Yin, Jiao-Jiao Zhang, Xian-Zhong Wang
Summary: This study shows that lead acetate exposure can impair reproductive function in male mice, including reducing sperm quality and inducing inflammatory damage. Oxidative stress and inflammatory response are key mechanisms underlying these adverse effects of lead acetate. Melatonin and vitamin C can serve as effective measures to counteract the toxic effects of lead acetate.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Review
Cell Biology
Ying-Hao Han, Xiao-Dong Liu, Mei-Hua Jin, Hu-Nan Sun, Taeho Kwon
Summary: Neurodegenerative diseases are neurological disorders characterized by progressive loss of neuronal structure and function. Recent studies have shown that NLRP3 inflammasome-mediated neuronal pyroptosis plays a crucial role in the pathogenesis of neurodegenerative diseases. Inhibiting NLRP3 inflammasome activation and attenuating neuronal pyroptosis could potentially serve as novel strategies for the treatment of neurodegenerative diseases.
INFLAMMATION RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Yuhan Yang, Yuan Wang, Shang Wei, Xianzhong Wang, Jiaojiao Zhang
Summary: Non-thermal plasma (NTP) is an ionized gas composed of reactive species, electric fields, and ultraviolet radiation. It can affect animal health by mediating reactive oxygen species (ROS) levels and has a wide range of potential applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Yijiao Wu, Shiyu Yu, Xiyin Zhang, Xianzhong Wang, Jiaojiao Zhang
Summary: Cold plasma, as an innovative technology, has a wide range of applications in oral treatment, tissue regeneration, wound healing, and cancer therapy. It regulates cell activity through the production of reactive oxygen species (ROS), promoting proliferation in skin-related cells and angiogenesis for wound healing but inhibiting cell proliferation in high levels. Cold plasma also has potential applications in animal husbandry, such as improving chicken growth and reproductive capacity and killing microorganisms on animal food surfaces. However, the molecular mechanism of cold plasma on cell activity and its applications in animal husbandry require further research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Veterinary Sciences
Taeho Kwon
Summary: Global viral outbreaks and diseases have had significant impacts on human health. Understanding disease mechanisms and developing effective vaccines and treatments are crucial. Non-human primates play a vital role as animal models, due to their evolutionary and physiological similarities to humans.
JOURNAL OF MEDICAL PRIMATOLOGY
(2024)
Article
Chemistry, Multidisciplinary
Tian Gao, Shengnan Guo, Jiaojiao Zhang, Jintao Chen, Shiru Yin, Na Peng, Qun Cai, Huan Xu, Yi Liu
Summary: The synthesis of low-cost and high-efficiency multicolour emitting graphene quantum dots (GQDs) from lignin has been successfully achieved in this study. The high crystallization and single-layered structure of the synthesized GQDs were confirmed using HRTEM. By analyzing XPS and FT-IR spectra, the formation mechanism of GQDs derived from lignin at the molecular level was investigated. Furthermore, the application of multicolour-emitting GQDs derived from lignin in LEDs was demonstrated, offering a new path for converting sustainable biomass into high-value nanomaterials.
Article
Medicine, Research & Experimental
Hu-Nan Sun, Dan-Ping Xie, Chen-Xi Ren, Xiao-Yu Guo, Hui-Na Zhang, Wan-Qiu Xiao, Ying-Hao Han, Yu-Dong Cui, Taeho Kwon
Summary: The study demonstrates that beta-CCE exhibits cytotoxicity against SiHa cells, inducing apoptosis in a time-and concentration-dependent manner by increasing ROS levels and activating the p38/MAPK signaling pathway. Additionally, the use of ROS scavenger NAC alleviates beta-CCE-induced apoptosis, while pre-treatment with p38 inhibitor SB203580 reduces apoptosis induced by beta-CCE.