Article
Multidisciplinary Sciences
Wooseob Kim, Julian Q. Zhou, Stephen C. Horvath, Aaron J. Schmitz, Alexandria J. Sturtz, Tingting Lei, Zhuoming Liu, Elizaveta Kalaidina, Mahima Thapa, Wafaa B. Alsoussi, Alem Haile, Michael K. Klebert, Teresa Suessen, Luis Parra-Rodriguez, Philip A. Mudd, Sean P. J. Whelan, William D. Middleton, Sharlene A. Teefey, Iskra Pusic, Jane A. O'Halloran, Rachel M. Presti, Jackson S. Turner, Ali H. Ellebedy
Summary: SARS-CoV-2 mRNA vaccination induces a persistent germinal center reaction in humans, resulting in affinity-matured long-term antibody responses that potently neutralize the virus.
Article
Biology
Marie Ghraichy, Valentin von Niederhausern, Aleksandr Kovaltsuk, Jacob D. Galson, Charlotte M. Deane, Johannes Truck
Summary: This study sequenced the IgH repertoire of different B cell subpopulations in the peripheral blood of healthy adults, revealing distinct characteristics at both individual sequence and repertoire levels through advanced bioinformatic analysis and machine learning. Sorted B cell subpopulations shared similar repertoire characteristics with their in silico separated subsets, and certain IgH repertoire characteristics correlated with the position of the constant region on the IgH locus. Overall, the study provides unprecedented insight into mechanisms of B cell repertoire control in peripherally circulating B cell subpopulations.
Article
Immunology
Christoph Ruschil, Gisela Gabernet, Constanze Louisa Kemmerer, Mohamed Ali Jarboui, Franziska Klose, Sven Poli, Ulf Ziemann, Sven Nahnsen, Markus Christian Kowarik
Summary: This study investigated the effects of cladribine treatment on B cell repertoire in multiple sclerosis patients. The results showed that cladribine therapy decreased memory B cell numbers and increased naive B cell numbers. However, it had minimal impact on the composition and diversity of immunoglobulins. The study also found that a proportion of B cell clones were maintained during treatment.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Zuhui Su, Yabin Jin, Yu Zhang, Zhanwen Guan, Huishi Li, Xiangping Chen, Chao Xie, Chuling Zhang, Xiaofen Liu, Peixian Li, Peiyi Ye, Lifang Zhang, Yaozhong Kong, Wei Luo
Summary: Membranous nephropathy (MN) is a common autoimmune glomerular disease that requires renal tissue biopsy for diagnosis, indicating the need for new diagnostic and prognostic monitoring methods. High-throughput sequencing analysis revealed abnormalities in multiple aspects of the immunoglobulin heavy chain in patients, with certain gene transcripts and parameters showing potential for predicting therapeutic effects.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Elizabeth R. Fraley, Santosh Khanal, Stephen H. Pierce, Cas A. LeMaster, Rebecca McLennan, Tomi Pastinen, Todd Bradley
Summary: Understanding the B cell response to SARS-CoV-2 vaccines is crucial, and this study sequenced the B cell receptor repertoire of individuals vaccinated with the Pfizer mRNA vaccine. The results showed that vaccine-induced IgG clonotypes had specific characteristics, including shorter heavy-chain complementarity determining region 3 (HCDR3) and higher somatic hypermutation (SHM). Shared clonotypes were also identified across individuals, indicating convergent evolution due to the vaccine independent of prior viral exposure.
Article
Biology
Haocheng Ye, Lin Cheng, Bin Ju, Gang Xu, Yang Liu, Shuye Zhang, Lifei Wang, Zheng Zhang
Summary: A specialized software SCIGA was developed for precise and quick analysis of 10X single-cell immunoglobulin repertoires, which helps researchers to interpret B-cell immunoglobulin repertoires with paired heavy and light chain. SCIGA successfully identified four neutralizing antibodies against SARS-CoV-2 in samples from patients with COVID-19.
Article
Immunology
Amy L. Kenter, Corey T. Watson, Jan-Hendrik Spille
Summary: During development, B and T lymphocytes generate diverse antigen receptors which allow organisms to respond to a wide range of pathogens. Studies suggest that genetic polymorphisms in the immunoglobulin heavy chain locus may influence immune-repertoire formation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Yi-Gen Pan, Benjamas Aiamkitsumrit, Laurent Bartolo, Yifeng Wang, Criswell Lavery, Adam Marc, Patrick Holec, C. Garrett Rappazzo, Theresa Eilola, Phyllis A. Gimotty, Scott E. Hensley, Rustom Antia, Veronika Zarnitsyna, Michael E. Birnbaum, Laura F. Su
Summary: The study found that in individuals not exposed to the virus, there is a substantial number of YFV-specific T cells, and after vaccination, rare populations with a reservoir of unexpanded TCRs generated robust responses. These altered dynamics reorganize the immunodominance hierarchy and result in an overall increase in higher avidity T cells.
Article
Cell Biology
Jong-Chan Park, Jinsung Noh, Sukjin Jang, Ki Hyun Kim, Hayoung Choi, Dongjoon Lee, Jieun Kim, Junho Chung, Dong Young Lee, Yonghee Lee, Hyunho Lee, Duck Kyun Yoo, Amos Chungwon Lee, Min Soo Byun, Dahyun Yi, Sun-Ho Han, Sunghoon Kwon, Inhee Mook-Jung
Summary: This study suggests that changes in B lymphocytes play a role in the development of Alzheimer's disease. The increase in B lymphocytes is associated with increased amyloid deposition in the brain and hyperactivation of microglia, leading to impaired clearance of beta-amyloid. Furthermore, despite high somatic hypermutation rates, patients with Alzheimer's disease share similar B cell receptor sequences.
Article
Immunology
Katharine Yu, Akshay Ravoor, Nuria Malats, Silvia Pineda, Marina Sirota
Summary: This study extracted B cell receptor repertoires from tumor and adjacent non-tumor samples and analyzed their diversity and network features. Differences in diversity and network statistics were observed across tumor types and subtypes, with primary tumors showing increased clonality. Significant associations were found between repertoire features and mutation load, tumor stage, and age, as well as survival in seven tumor types.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Akshay J. Patel, Naeem Khan, Alex Richter, Babu Naidu, Mark T. Drayson, Gary W. Middleton
Summary: B cells play a key role in lung cancer pathogenesis and response to immunotherapy. The B cell repertoire differs between tumor and circulation, with a diverse range of plasma cell and memory cell phenotypes in lung cancer. The influence of smoking and tumor type on the B cell repertoire is limited.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Brian L. Le, Renan Sper, Sandra C. A. Nielsen, Silvia Pineda, Quoc-Hung Nguyen, Ji-Yeun Lee, Scott D. Boyd, Tippi C. MacKenzie, Marina Sirota
Summary: Preterm labor is the leading cause of neonatal morbidity and mortality worldwide. The study found that preterm infants display immature features and clonal overlap with maternal TCR-beta clones. In preterm pregnancies, there is significantly higher clonal convergence between mother and baby pairs compared to term pregnancies.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Biochemical Research Methods
Catherine Sutherland, Graeme J. M. Cowan
Summary: Adaptive Immune Receptor Repertoire Sequencing is a rapidly developing field that has improved our understanding of the adaptive immune system. We have developed AIRRSHIP, a Python package that produces synthetic human B cell receptor sequences, which can be used to assess the accuracy of repertoire analysis tools and identify factors contributing to inaccuracies in results.
Article
Cell Biology
Prasanti Kotagiri, Federica Mescia, William M. Rae, Laura Bergamaschi, Zewen K. Tuong, Lorinda Turner, Kelvin Hunter, Pehuen P. Gerber, Myra Hosmillo, Christoph Hess, Menna R. Clatworthy, Ian G. Goodfellow, Nicholas J. Matheson, Eoin F. McKinney, Mark R. Wills, Ravindra K. Gupta, John R. Bradley, Rachael J. M. Bashford-Rogers, Paul A. Lyons, Kenneth G. C. Smith
Summary: B cell receptor (BCR) repertoire development differs between severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection and vaccination, potentially informing vaccine strategies. After infection, there is an increase in immunoglobulin (Ig)G1/3 and IgA1 BCRs, a decrease in somatic hypermutation (SHM), and expansion of IgM and IgA clones in severe disease. In contrast, vaccination leads to an increase in IgD/M BCRs, unchanged SHM, and prominent expansion of IgG clones.
Article
Hematology
Yohei Funakoshi, Kimikazu Yakushijin, Goh Ohji, Takaji Matsutani, Wataru Hojo, Hironori Sakai, Sakuya Matsumoto, Marika Watanabe, Akihito Kitao, Yasuyuki Saito, Shinichiro Kawamoto, Katsuya Yamamoto, Taiji Koyama, Yoshiaki Nagatani, Shiro Kimbara, Yoshinori Imamura, Naomi Kiyota, Mitsuhiro Ito, Hironobu Minami
Summary: The use of tixagevimab/cilgavimab is an important strategy to protect immunocompromised patients with haematological malignancies from COVID-19. However, it can mask the production of anti-spike antibody after vaccination, making it difficult to assess vaccine response. A new quantification method using B-cell receptor (BCR) repertoire assay and the Coronavirus Antibody Database (CoV-AbDab) has been established to assess the response to SARS-CoV-2 vaccination at mRNA level. The fluctuation in matching sequences can be analyzed to evaluate the postvaccine immune response.
BRITISH JOURNAL OF HAEMATOLOGY
(2023)
Article
Allergy
Andrea A. Mauracher, Julia J. M. Eekels, Janine Woytschak, Audrey van Drogen, Alessandra Bosch, Seraina Prader, Matthias Felber, Maximillian Heeg, Lennart Opitz, Johannes Trueck, Silke Schroeder, Eva Adank, Adam Klocperk, Eugenia Haralambieva, Dieter Zimmermann, Sofia Tantou, Kosmas Kotsonis, Aikaterini Stergiou, Maria G. Kanariou, Stephan Ehl, Onur Boyman, Anna Sediva, Raffaele Renella, Markus Schmugge, Stefano Vavassori, Jana Pachlopnik Schmid
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2020)
Article
Immunology
Patrick M. Meyer Sauteur, Johannes Truck, Annemarie M. C. van Rossum, Christoph Berger
JOURNAL OF INFECTIOUS DISEASES
(2020)
Review
Pediatrics
Michael Buettcher, Johannes Trueck, Anita Niederer-Loher, Ulrich Heininger, Philipp Agyeman, Sandra Asner, Christoph Berger, Julia Bielicki, Christian Kahlert, Lisa Kottanattu, Patrick M. Meyer Sauteur, Paolo Paioni, Klara Posfay-Barbe, Christa Relly, Nicole Ritz, Petra Zimmermann, Franziska Zucol, Rita Gobet, Sandra Shavit, Christoph Rudin, Guido Laube, Rodo von Vigier, Thomas J. Neuhaus
Summary: In children, UTI is commonly caused by kidney and urinary tract anomalies, and bladder-bowel dysfunction. Treatment should consider age and symptoms for appropriate antimicrobial selection.
EUROPEAN JOURNAL OF PEDIATRICS
(2021)
Editorial Material
Immunology
Luregn J. Schlapbach, Johannes Truck, Thierry Roger
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Immunology
Marie Ghraichy, Jacob D. Galson, Aleksandr Kovaltsuk, Valentin von Niederhausern, Jana Pachlopnik Schmid, Mike Recher, Annaise J. Jauch, Enkelejda Miho, Dominic F. Kelly, Charlotte M. Deane, Johannes Trueck
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Oncology
Sara Pischedda, Daniel O'Connor, Benjamin P. Fairfax, Antonio Salas, Federico Martinon-Torres, Andrew J. Pollard, Johannes Truck
Summary: Epigenetic changes during early childhood may be associated with antigen-specific antibody responses to pneumococcal vaccines, with differences in methylation patterns observed between high and low vaccine responders. Pathways involved in the regulation of cell-cell adhesion and T cell activation were found to be enriched in the differentially methylated positions identified between 12 and 24 months of age.
CLINICAL EPIGENETICS
(2021)
Review
Biology
Johannes Truck, Anne Eugster, Pierre Barennes, Christopher M. Tipton, Eline T. Luning Prak, Davide Bagnara, Cinque Soto, Jacob S. Sherkow, Aimee S. Payne, Marie-Paule Lefranc, Andrew Farmer, Magnolia Bostick, Encarnita Mariotti-Ferrandiz
Summary: The use of adaptive immune receptor repertoire sequencing (AIRR-seq) has provided new insights into the immune system but also comes with challenges. The AIRR Community's Biological Resources Working Group conducted a survey to understand the need for standards and controls in generating and annotating AIRR-seq data, and offered recommendations for developing AIRR-seq standards and controls based on the survey results.
Article
Biology
Marie Ghraichy, Valentin von Niederhausern, Aleksandr Kovaltsuk, Jacob D. Galson, Charlotte M. Deane, Johannes Truck
Summary: This study sequenced the IgH repertoire of different B cell subpopulations in the peripheral blood of healthy adults, revealing distinct characteristics at both individual sequence and repertoire levels through advanced bioinformatic analysis and machine learning. Sorted B cell subpopulations shared similar repertoire characteristics with their in silico separated subsets, and certain IgH repertoire characteristics correlated with the position of the constant region on the IgH locus. Overall, the study provides unprecedented insight into mechanisms of B cell repertoire control in peripherally circulating B cell subpopulations.
Article
Immunology
Else M. Bijker, Elizabeth A. L. Bateman, Johannes Truck, Smita Patel, Dominic F. Kelly
Summary: This study retrospectively investigated 68 children with invasive pneumococcal disease (IPD) and found clinically relevant abnormal immunological findings in 4 out of 51 children (8%). The results support the recommendation to perform immunological investigations in children with IPD for necessary preventive measures and follow-up.
PEDIATRIC INFECTIOUS DISEASE JOURNAL
(2022)
Editorial Material
Pediatrics
Veronika Baghin, Seraina Prader, Selma Sirin, Jana Pachlopnik Schmid, Johannes Trueck
ARCHIVES OF DISEASE IN CHILDHOOD-EDUCATION AND PRACTICE EDITION
(2023)
Letter
Immunology
Maarja Soomann, Seraina Prader, Jana Pachlopnik Schmid, Tayfun Gungor, Johannes Truck
JOURNAL OF CLINICAL IMMUNOLOGY
(2023)
Article
Immunology
Sophie Strasser, Christa Relly, Christoph Berger, Johannes Truck
Summary: A retrospective analysis found that a substantial proportion of children with severe bacterial infections (SBIs) had immune function impairments. Routine immunological testing can help identify these abnormalities and optimize preventive measures to avoid future SBI episodes.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Medicine, Research & Experimental
Henderson Zhu, Irina Chelysheva, Deborah L. Cross, Luke Blackwell, Celina Jin, Malick M. Gibani, Elizabeth Jones, Jennifer Hill, Johannes Truck, Dominic F. Kelly, Christoph J. Blohmke, Andrew J. Pollard, Daniel O'Connor
Summary: This study compares the immune responses and protection effects of two typhoid vaccines, ViPS and ViTT, through the analysis of genomic data. The study reveals distinct molecular features between the two vaccines, mainly related to humoral immune responses. Furthermore, the study identifies molecular correlates of protection against S. Typhi infection. These findings have important implications for future vaccine design and assessment.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Biochemistry & Molecular Biology
Johannes Trueck, Mirjam van der Burg
CURRENT OPINION IN SYSTEMS BIOLOGY
(2020)
Article
Medicine, General & Internal
Truck Johannes, Prader Seraina, Natalucci Giancarlo, Hagmann Cornelia, Brotschi Barbara, Kelly Janet, Bassler Dirk, Steindl Katharina, Rauch Anita, Baumgartner Matthias, Fingerhut Ralph, Hauri-Hohl Mathias, Gungor Tayfun, Schmid Jana Pachlopnik, Berger Christoph, Reichenbach Janine
SWISS MEDICAL WEEKLY
(2020)