期刊
BMC IMMUNOLOGY
卷 19, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s12865-018-0267-7
关键词
Echinococcus granulosus protoscoleces; Excretory-secretory products; B10 cells; TLR-2; PTEN; PI3K
类别
资金
- National Natural Science Foundation of China [81501762, 81772224, 81871670]
- Laboratory of Parasite and Vector Biology, MOH, China [WSBKTKT201502]
- China Postdoctoral Science Foundation [2015 M581864]
- Jiangsu Qing Lan Project
- Top-notch Academic Programs Project of Jiangsu Higher Education Institutions (TAPP)
- Priority Academic Program Development of Jiangsu Higher Education Institutions
BackgroundExcretory-secretory products released by Echinococcus granulosus protoscoleces (EgPSC-ESPs) are well-known to regulate T cell responses. However, their direct influence on the differentiation of B cell subsets remains largely elusive. This study investigated the effects of EgPSC-ESPs on the differentiation of IL-10-producing B cells (B10), and explored the possible role of Toll-like receptor 2 (TLR-2) signaling in this process.ResultsIn comparison to phosphate buffered saline (PBS), B cells exposed to the excretory-secretory products (ESPs) generated higher percentages of B10 cells, with higher expression of IL-10 mRNA, and larger amount of IL-10 production, which were in a dose dependent way. The mRNA and protein expression of TLR-2 in the ESPs-stimulated B cells were significantly higher than those in PBS, which was consistent to the results in B cells isolated from EgPSC infected mice. Moreover, TLR-2(-/-) B cells in response to ESPs stimulation expressed lower levels of IL-10 mRNA and produced undetectable IL-10 in comparison to those in normal B cells. In addition, Phosphatase and tensin homolog deleted on chromosome ten/AKT/Phosphatidylinositol-3 kinase (PTEN/AKT/PI3K) pathway was activated in ESPs-treated B cells, which was also dependent on TLR-2 signaling. Pam3CSK4, the agonist of TLR-2, could mock the effects of ESPs on the expression of PTEN, AKT and PI3K.ConclusionOverall, this study revealed that TLR-2 signaling was required for B10 induction mediated by EgPSC-ESPs, which might be an immunomodulatory target against the parasite infection.
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