Article
Agronomy
Tingting Liu, Baolong Tao, Hanfei Wu, Jing Wen, Bin Yi, Chaozhi Ma, Jinxing Tu, Tingdong Fu, Lixia Zhu, Jinxiong Shen
Summary: Loss of function of the chloroplast development-related gene Bn.YCO affected plastid gene transcription in rapeseed seedlings, influencing chloroplast biogenesis.
Article
Biotechnology & Applied Microbiology
Shan Tang, Ning Guo, Qingqing Tang, Fei Peng, Yunhao Liu, Hui Xia, Shaoping Lu, Liang Guo
Summary: The study highlights the important role of BnaBASS2 in seed lipid metabolism and accumulation in Brassica napus, showing that gene manipulation can alter metabolites in seeds and influence lipid synthesis.
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Article
Biology
Liangqian Yu, Dongxu Liu, Feifan Yin, Pugang Yu, Shaoping Lu, Yuting Zhang, Hu Zhao, Chaofu Lu, Xuan Yao, Cheng Dai, Qing-Yong Yang, Liang Guo
Summary: This study reveals the transcriptional regulation between lipid and phenylpropane accumulation during Brassica napus seed development. Co-expression network analysis and correlation analysis identified candidate genes that may regulate seed oil content.
Article
Plant Sciences
Ka Zhang, Fei Liu, Zhixin Wang, Chenjian Zhuo, Kaining Hu, Xiaoxia Li, Jing Wen, Bin Yi, Jinxiong Shen, Chaozhi Ma, Tingdong Fu, Jinxing Tu
Summary: In this study, it was found that oilseed rape enhances its resistance against Sclerotinia infection through a conserved MAPK cascade-mediated phosphorylation of BnWRKY33. Additionally, the induction of BnaA03.WRKY28 and BnaA09.VQ12 in the later stages of infection suppresses BnWRKY33 expression but promotes branching growth. These findings highlight the adaptability of oilseed rape in response to lethal pathogens, as it modulates its defense-response strength while balancing growth.
Article
Biochemistry & Molecular Biology
Stepan Fenyk, Helen K. Woodfield, Trevor B. Romsdahl, Emma J. Wallington, Ruth E. Bates, David A. Fell, Kent D. Chapman, Tony Fawcett, John L. Harwood
Summary: This study investigated the effects of expressing PDAT1 in oil seeds on lipid metabolism, showing that PDAT1 overexpression results in a slight decrease in seed oil content and altered fatty acid composition. This has important implications for regulating oil accumulation in crops.
BIOCHEMICAL JOURNAL
(2022)
Article
Cell Biology
Yue Hong, Hui Xia, Xiao Li, Ruyi Fan, Qing Li, Zhewen Ouyang, Shan Tang, Liang Guo
Summary: This study investigates the important functions of two conserved genes, BnaC06.NTT1b and BnaA07.NTT1a, in Brassica napus. These genes play a vital role in regulating ATP/ADP homeostasis in plastids and have significant effects on glycolysis and membrane lipid composition.
Article
Agronomy
Yuqing Li, Usman Ali, Zhouxiao Cao, Chenghao Zeng, Mengying Xiao, Fang Wei, Xuan Yao, Liang Guo, Shaoping Lu
Summary: The BnaA09.FAX1 gene plays an important role in improving seed oil accumulation and plant growth in Brassica napus, while the function of BnaFAX1 may be compensated by other homologous genes of BnaFAX1 and other BnaFAX genes in the mutants.
MOLECULAR BREEDING
(2022)
Article
Agronomy
Hongbo Chao, Liangxing Guo, Weiguo Zhao, Huaixin Li, Maoteng Li
Summary: This study identified a major QTL, cqSC-A09, on chromosome A09 that controls seed color, oil content, and fiber content simultaneously in Brassica napus. This QTL has a large effect and can increase the oil content and reduce pigment and fiber content in seeds. The study also identified important candidate genes for cqSC-A09, which provides a foundation for further cloning and breeding applications. The results of this study have significant implications for breeding high oil quality rapeseed varieties.
THEORETICAL AND APPLIED GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Saiyu Wang, Chengcheng Bai, Na Luo, Youwei Jiang, Yulu Wang, Yu Liu, Chunjie Chen, Yuxin Wang, Qiaoqiao Gan, Shurong Jin, Yu Ni
Summary: A transcriptional regulator, BnaC9.DEWAX1, was found to negatively regulate wax biosynthesis in Brassica napus by directly interacting with the promoter of BnCER1-2. It was predominantly expressed in leaves and siliques and its expression was influenced by hormones and abiotic stresses. Overexpression of BnaC9.DEWAX1 altered cuticular wax composition and increased epidermal permeability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Hanna Marie Schilbert, Maximilian Schoene, Thomas Baier, Mareike Busche, Prisca Viehoever, Bernd Weisshaar, Daniela Holtgraewe
Summary: It was found that there are two homologous genes in the flavonol synthase gene family of B. napus, which have dual enzyme activity and play important roles in the accumulation of flavonols in seeds.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Huailin Li, Kaidi Yu, Zilu Zhang, Yalun Yu, Jiakai Wan, Hanzi He, Chuchuan Fan
Summary: This study targeted key genes in the flavonoid biosynthesis pathway in rapeseed using CRISPR/Cas9 and found that the resulting mutations led to changes in seed coat color, oil content, and fatty acid composition. Gene expression analysis provided insights into the mechanisms underlying these changes. The study generated valuable resources for breeding programs.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Agronomy
Chuanji Zhao, Luqman Bin Safdar, Meili Xie, Meijuan Shi, Zhixue Dong, Li Yang, Xiaohui Cheng, Yueying Liu, Zetao Bai, Yang Xiang, Chaobo Tong, Junyan Huang, Lijiang Liu, Shengyi Liu
Summary: A yellowish-white flower mutant was obtained from oilseed rape through EMS treatment, and genetic analysis revealed that this trait was controlled by a single recessive gene. Through BSA-seq and KASP, the ywf locus on chromosome A08 was cloned, with further investigation showing a C-to-T substitution in the coding region leading to a premature translation termination in the ywf mutant.
Article
Biochemistry & Molecular Biology
Jun-Lan Xiong, Ni Ma
Summary: This study investigates the molecular and metabolic mechanisms underlying the effect of fullerol, a carbon nanoparticle, on improving drought tolerance in Brassica napus. The results show that fullerol application alters the molecular and metabolic profiles of B. napus. Genes and metabolites related to carbohydrate metabolism, amino acid metabolism, and secondary metabolite metabolism are differentially expressed or accumulated in response to drought or fullerol treatment. These findings suggest that fullerol can enhance drought tolerance in B. napus by regulating the accumulation of carbohydrates, amino acids, and secondary metabolites.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Bo Hong, Bingqian Zhou, Zechuan Peng, Mingyao Yao, Junjie Wu, Xuepeng Wu, Chunyun Guan, Mei Guan
Summary: During the growth period of rapeseed, waterlogging stress can significantly affect its growth. In this study, the waterlogging-tolerant rapeseed line G230 and the waterlogging-sensitive rapeseed line G218 were identified through experiments. The results showed that G230 had lower leaf aging and root damage than G218 under waterlogging stress. Further analysis revealed that flavonoid biosynthesis and vitamin B6 metabolism played a key role in the waterlogging tolerance of rapeseed. These findings provide new insights for improving the waterlogging tolerance of rapeseed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agronomy
Hongli Dong, Lei Yang, Yilin Liu, Guifu Tian, Huan Tang, Shuangshuang Xin, Yixin Cui, Qing Xiong, Huafang Wan, Zhi Liu, Christian Jung, Wei Qian
Summary: This study constructed a gene coexpression network in the early developmental stage of rapeseed seeds and identified gene modules related to seed weight regulation. A key gene, SCPL19, was found through candidate gene association analysis and functionally investigated using Arabidopsis. The study expands our understanding of the molecular mechanism for seed weight regulation in rapeseed.
Article
Biochemistry & Molecular Biology
Yanqing Sun, Enhui Shen, Yiyu Hu, Dongya Wu, Yu Feng, Sangting Lao, Chenfeng Dong, Tianyu Du, Wei Hua, Chu-Yu Ye, Jinhuan Zhu, Qian-Hao Zhu, Daguang Cai, Lidia Skuza, Jie Qiu, Longjiang Fan
Summary: Rye, an important crop and valuable genetic resource, was found to be domesticated directly from weedy relatives through genomic selection by humans, according to a study analyzing whole-genome resequencing data from worldwide accessions of wild, weedy, and cultivated rye. The study also identified genes associated with important agronomic traits and detected a potential introgression from a wild perennial that could affect grain yield and plant growth in rye.
Article
Plant Sciences
Qian-Hao Zhu, Shuangxia Jin, Yuman Yuan, Qing Liu, Xianlong Zhang, Iain Wilson
Summary: This study successfully generated a collection of cotton miR482 mutants using CRISPR/Cas9 technology, resulting in lower disease index in response to pathogen infection. The study provides a valuable resource for the cotton community to uncover the role of miR482-NLR module(s) in the interaction between cotton and different pathogens.
Article
Plant Sciences
Fan Yang, Yongchao Han, Qian-Hao Zhu, Xinyu Zhang, Fei Xue, Yanjun Li, Honghai Luo, Jianghong Qin, Jie Sun, Feng Liu
Summary: The study conducted comparative phenotypic and transcriptomic analyses of two Gossypium hirsutum varieties tolerant (XL22) or sensitive (XL17) to drought stress. It was found that XL17 consumed more water than XL22 under water deficit conditions. Water deficit significantly induced accumulation of major wax components and cutin monomers in leaves of both varieties, but accumulation of major cutin monomers was repressed in both.
Article
Agriculture, Multidisciplinary
Xue-feng Wang, Dong-nan Shao, Qian Liang, Xiao-kang Feng, Qian-hao Zhu, Yong-lin Yang, Feng Liu, Xin-yu Zhang, Yan-jun Li, Jie Sun, Fei Xue
Summary: In this study, the researchers identified a dwarf-red cotton mutant that is a gibberellin-sensitive mutant caused by a mutation in the GhDR gene. The mutation resulted in a frameshift and truncation of the GhDR protein, leading to altered subcellular localization. Transcriptome analysis revealed downregulation of key genes involved in gibberellin biosynthesis and upregulation of genes related to gibberellin degradation and anthocyanin biosynthesis pathway in the mutant. This study provides insights into the molecular basis of plant architecture and anthocyanin accumulation regulation in cotton.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2023)
Article
Agricultural Engineering
Qian Liang, Yanlong Jin, Qian-Hao Zhu, Dongnan Shao, Xuefeng Wang, Xiaohu Ma, Feng Liu, Xinyu Zhang, Yanjun Li, Jie Sun, Fei Xue
Summary: This study characterized a cotton accession with pink petals and red spots at the base of petals, which is derived from a wild cotton species from Australia. The gene responsible for the red spots was identified and found to be important for hybrid seed production and pollinator attraction in cotton.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Plant Sciences
Weijie Xu, Yue Wang, Jianbo Xie, Shuxian Tan, Haofei Wang, Yiyang Zhao, Qing Liu, Yousry A. El-Kassaby, Deqiang Zhang
Summary: Soil salinity affects crop productivity and triggers salt stress in plants. This study found that the growth-regulating factor 15 (PagGRF15) and its target gene high-affinity K+ transporter 6 (PagHAK6) play important roles in the salt stress response. Overexpression of PagGRF15 and PagHAK6 improved salt tolerance in poplar by enhancing Na+ transport and regulating H2O2 accumulation.
Article
Plant Sciences
Lihua Chen, Bin Chen, Qian-Hao Zhu, Xinyu Zhang, Tiange Sun, Feng Liu, Yonglin Yang, Jie Sun, Yanjun Li
Summary: In this study, sugar transporter genes in V. dahliae were identified and their roles in growth and pathogenicity were investigated. VdST3 and VdST12 were found to be important for V. dahliae's growth and pathogenicity, and asiRNA was a valuable tool for functional characterization of V. dahliae genes.
FRONTIERS IN PLANT SCIENCE
(2023)
Editorial Material
Plant Sciences
Enhui Shen, Jun Zou, Jiandong Bao, Qian-Hao Zhu
FRONTIERS IN PLANT SCIENCE
(2023)
Editorial Material
Biochemistry & Molecular Biology
Qian-Hao Zhu
Article
Biochemistry & Molecular Biology
Yue Sun, Yifei Han, Kuang Sheng, Ping Yang, Yuefen Cao, Huazu Li, Qian-Hao Zhu, Jinhong Chen, Shuijin Zhu, Tianlun Zhao
Summary: The comprehensive utilization of cottonseeds is limited by the presence of pigment glands and gossypol. This study investigated the development of pigment glands in G. bickii, a wild cotton species, and identified the regulatory mechanisms involved. Single-cell RNA sequencing revealed the presence of distinct cell populations, including pigment gland cells, secretory cells, and apoptotic cells. Light and gibberellin were found to promote pigment gland formation, and three novel genes were identified to affect this process. These findings provide new insights into pigment gland morphogenesis and lay the foundation for future scRNA-seq investigations in cotton.
Article
Plant Sciences
Mengjiao Jia, Shuaishuai Chen, Qianhao Zhu, Ruiting Zheng, Yanjun Li, Jie Sun, Feng Liu
Summary: The GhGRL28 gene plays an important role in salt tolerance in cotton plants, and its silencing leads to poor growth and increased oxidative stress under salt stress conditions. These results suggest that GhGRL28 actively regulates redox reactions induced by stress and is crucial for maintaining the growth and development of cotton plants under stress conditions.
PLANT GROWTH REGULATION
(2023)
Article
Plant Sciences
Lei Zhang, Yajie Wu, Yongang Yu, Yihao Zhang, Feng Wei, Qian-Hao Zhu, Jinglong Zhou, Lihong Zhao, Yalin Zhang, Zili Feng, Hongjie Feng, Jie Sun
Summary: Protein lysine acetylation is an important post-translational modification mechanism in cellular regulation in eukaryotes. Calmodulin (CaM) is a crucial Ca2+ sensor in eukaryotes and plays a key role in plant immunity. This study shows that GhCaM7, an acetylated protein, is a positive regulator of Verticillium dahliae resistance in cotton and Arabidopsis plants.
Article
Agricultural Engineering
Youzhong Li, Xinyu Zhang, Zhongxu Lin, Qian-Hao Zhu, Yanjun Li, Fei Xue, Shuaishuai Cheng, Hongjie Feng, Jie Sun, Feng Liu
Summary: In this study, two chromosome segment substitution lines (CSSLs) showing stable resistance to Verticillium wilt (VW) were identified, and the transcriptome analysis revealed genes associated with cotton response to V. dahliae infection.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Automation & Control Systems
Zijie Shen, Enhui Shen, Qian-Hao Zhu, Longjiang Fan, Quan Zou, Chu-Yu Ye
Summary: This article introduces a genomic descriptor, GSCtool, for phenotype prediction based on the genome. Compared to genotype matrices, GSCtool significantly reduces model training time and improves prediction accuracy. Additionally, GSCtool also achieves good performance in variety identification, which is beneficial for crop variety protection.
ADVANCED INTELLIGENT SYSTEMS
(2023)