Article
Chemistry, Physical
Hoi Ting Wong, Alexander Romaschin, Sara Bjelobrk, Brian De La Franier, Michael Thompson
Summary: This study demonstrates that PMB-modified glass beads can competitively reduce the presence of LPS, and suggests that monomerization of LPS may be the mechanism by which its toxicity is reduced.
Article
Chemistry, Multidisciplinary
Yu-Ci Chang, Zhicheng Jin, Ke Li, Jiajing Zhou, Wonjun Yim, Justin Yeung, Yong Cheng, Maurice Retout, Matthew N. Creyer, Pavla Fajtova, Tengyu He, Xi Chen, Anthony J. O'Donoghue, Jesse V. Jokerst
Summary: Electrostatic interactions play a crucial role in colloidal assembly. The Schulze-Hardy rule states that nanoparticles tend to coagulate more when exposed to counterions with higher charge valence. This study investigates how the Schulze-Hardy rule functions when using more complex charge carriers instead of simple electrolytes. The researchers designed cationic peptides with different valencies and found that their charge screening behaviors on anionic gold nanoparticles followed the six-power relationship in the Schulze-Hardy rule. This discovery also led to a simple and effective strategy for visually measuring SARS-CoV-2 main protease (M-pro). By understanding the fundamental disassembly of nanoparticles based on the Schulze-Hardy rule, this work offers potential in designing versatile substrates for colorimetric sensing of other proteases.
Article
Biochemistry & Molecular Biology
Jiajun Jiang, Bingna Huang, Ningjun Li, Changcheng An, Changjiao Sun, Yue Shen, Ravi Gooneratne, Haixin Cui, Shenshan Zhan, Yan Wang
Summary: A colorimetric assay based on LPS binding aptamer and gold nanoparticles was developed for rapid and accurate detection of LPS concentration, which contributes to the protection of food safety and human health.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Materials Science, Biomaterials
Andreea Campu, Ilinca Muresan, Monica Potara, Diana Raluca Lazar, Florin-Leontin Lazar, Simona Cainap, Dan Mircea Olinic, Dana Maniu, Simion Astilean, Monica Focsan
Summary: This study develops a microfluidic plasmonic chip that can efficiently and accurately detect the cardiac troponin I biomarker via three complementary detection techniques. The study also provides a better understanding of the thermoplasmonic biosensing mechanism using gold nanoparticles' photothermal properties.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Engineering, Electrical & Electronic
Allwyn S. Rajamani, Hariharan Manoharan, Christina Grace Danny, P. V. N. Kishore, V. V. R. Sai
Summary: This study presents a ray-optics based model for the multimode U-bent silica fiber optic refractive index (RI) sensor probes. Experimental investigation was conducted to determine the optimum fiber core and bend diameter, as well as the optimal bend ratio. The results showed that U-bent probes with a specific core diameter exhibited higher RI sensitivity within a certain range of bend ratios. Further investigations using wet chemical step-etching of the silica fiber core showed improved RI sensitivity until a certain waist diameter, followed by a drop. A compact LED-photodetector based dip-type handheld refractometer was also demonstrated for potential analytical applications.
SENSORS AND ACTUATORS A-PHYSICAL
(2022)
Article
Optics
Fan He, Zewei Luo, Zhixi Wen, Haojie Huangfu, Yanting Feng, Xinyuan Qi, Yixiang Duan
Summary: The study demonstrates the use of a gold nanoparticle-decorated silver film (AuNPs-AgFM) as a coating in bent fiber optic surface plasmonic resonance (SPR) for integrated cytosensor and phototherapy. Optimization of parameters results in a high amplification sensitivity, wavelength sensitivity, and surface temperature for the designed SPR biosensor. This research holds significant importance for advancing the clinical application of fiber optics.
Article
Spectroscopy
Hyeong-Min Kim, Hyo-Jun Kim, Jae-Hyoung Park, Seung-Ki Lee
Summary: In this study, gold-palladium bimetallic nanodisks were successfully patterned on optical fibers, exhibiting high refractive index sensitivity and excellent hydrogen sensing performance. The fiber-optic plasmonic nanoprobe, based on nanosphere lithography and chemical growth, has the advantages of high throughput and low cost, making it suitable for remote sensing to detect dangerous environments in the clean energy era.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Chemistry, Analytical
Mona Soroush, Walid Ait Mammar, Axel Wilson, Hedayatollah Ghourchian, Michele Salmain, Souhir Boujday
Summary: We developed a magneto-plasmonic biosensor for immunodetection of antigens in small sample volumes. The biosensor utilized gold nanoparticles and magnetic beads conjugated with antibodies as capture and detection probes, respectively. The sandwich immunocomplex formed by the target analyte and detection probe was separated using a magnetic field, and the signal was measured by UV-Visible spectroscopy. The assay was successfully applied in a microfluidic system for quantification of the analyte.
Article
Medicine, Research & Experimental
Neeltje Steeghs, Aaron R. Hansen, Glenn J. Hanna, Elena Garralda, Haeseong Park, James Strauss, Michael Adam, Gossett Campbell, Jennifer Carver, Rachael Easton, Katherine Mays, Peter Skrdla, Herbert Struemper, Michael L. Washburn, Christopher Matheny, Sarina A. Piha-Paul
Summary: This study evaluated the safety and efficacy of GSK1795091, a Toll-like receptor 4 (TLR4) agonist, in combination with immunotherapy in patients with solid tumors. The modified formulation of GSK1795091 showed changes in biological activity compared to the original formulation, but the safety profile was similar between the two formulations.
CTS-CLINICAL AND TRANSLATIONAL SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Hiroshi Kimoto, Yota Suzuki, Yu Ebisawa, Masamitsu Iiyama, Takeshi Hashimoto, Takashi Hayashita
Summary: In this study, a simple and rapid method for detecting endotoxin was developed using a dpa-modified pyrenyl probe. The probe exhibited fluorescence in the presence of LPS and had high sensitivity and selectivity for LPS. This probe has the potential to be used as a chemical sensor for the detection of endotoxin in medical and pharmaceutical applications.
Article
Chemistry, Multidisciplinary
Giulia Santopolo, Antonio Clemente, Maria Aranda, Antonia Socias, Alberto Del Castillo, Aniceta Chica, Marcio Borges, Roberto de la Rica
Summary: The proposed plasmonic assay utilizes the aggregation of gold nanoparticles with cationic granule proteins to detect the degranulation status of septic cells colorimetrically, enabling easy detection of sepsis cells with fewer granules. This method shows high specificity and diagnostic performance, making it a promising alternative for diagnosing sepsis in decentralized healthcare schemes.
Article
Biophysics
Yu Li, Xu Wang, Wei Ning, Enlai Yang, Yongxin Li, Zewei Luo, Yixiang Duan
Summary: The development of rapid and sensitive detection methods for pathogenic bacteria is crucial for disease therapy and prevention. In this study, a sandwich method based on omega-FOLSPR was constructed, demonstrating excellent selectivity and performance in detecting bacteria.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biochemistry & Molecular Biology
Weirong Wei, Yinyan Tang, Huimin He, Subash C. B. Gopinath, Lingling Wang
Summary: Acute myocardial infarction (AMI) patients show significantly elevated C-reactive protein (CRP) levels, and the concentration of CRP variations help identify conditions associated with AMI. Plasmonic enzyme-linked immunosorbent assay (ELISA) allows for naked-eye CRP detection based on CRP aggregation, achieving the lowest limit of CRP detection.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2022)
Article
Chemistry, Analytical
Zeeshan A. Khan, Seungkyung Park
Summary: In this study, a colorimetric assay based on the binding of Aβ(1-40) to gold nanoparticles and metal ions was proposed for the detection of Aβ. This newly developed assay showed good linearity and high sensitivity for the detection of Aβ(1-40) in biological or biomedical samples.
Article
Immunology
Daniel Hatlem, Mikkel Christensen, Nina K. Broeker, Per E. Kristiansen, Reidar Lund, Stefanie Barbirz, Dirk Linke
Summary: Alpha-helical coiled-coils are common protein structures found in all living organisms. Modified coiled-coil sequences have been widely used in biotechnology, vaccine development, and biochemical research to induce protein oligomerization and form self-assembled protein scaffolds. In this study, a trimeric variant of a peptide derived from the yeast transcription factor GCN4, called GCN4-pII, was shown to bind bacterial lipopolysaccharides (LPS) with high affinity. LPS molecules are toxic glycolipids found in the outer membrane of Gram-negative bacteria. The researchers also demonstrated the ability of GCN4-pII to break down LPS micelles in solution. These findings suggest that GCN4-pII and its derivatives could be used for novel LPS detection and removal solutions with important applications in the production and quality control of biopharmaceuticals and other biomedical products, where even trace amounts of residual LPS can be deadly.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)