Article
Biochemistry & Molecular Biology
Isabel Fraile-Gutierrez, Susana Iglesias, Niuris Acosta, Julia Revuelta
Summary: This research developed a new hydrogel that can protect beta-galactosidase from degradation in the stomach and retain its activity in the intestine. It represents a promising approach for oral transport of the enzyme to reduce the symptoms of hypolactasia.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Food Science & Technology
Zhenshang Xu, Can Li, Yanxin Ye, Ting Wang, Susu Zhang, Xinli Liu
Summary: Transcriptome sequencing revealed the gene expression difference of Lactiplantibacillus plantarum in glucose and lactose medium, identifying that beta-galactosidase LacLM plays a major role in lactose utilization, providing a theoretical foundation for the growth and metabolism of Lp. plantarum in lactose medium.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Polymer Science
J. Lucas Vallejo-Garcia, Ana Arnaiz, Maria D. Busto, Jose M. Garcia, Safil Vallejos
Summary: This study presents the synthesis and characterization of a polyacrylic film material for enzyme immobilization. The material achieves efficient immobilization of the enzyme through the formation of azo linkages and demonstrates superior performance in lactose hydrolysis.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Agricultural Engineering
Kevin Munoz, Diego Ahumada, Felipe Arenas, Cecilia Guerrero, Andres Illanes, Carlos Vera
Summary: The study found that butyl-beta-galactoside is significantly more soluble in the organic phase than lactose and monosaccharides. Additionally, higher values of the 1-butanol/aqueous phase ratio were shown to reduce secondary hydrolysis and increase yields. Interfacial properties for butyl-beta-galactoside were also determined at 25 degrees C.
BIORESOURCE TECHNOLOGY
(2021)
Article
Chemistry, Physical
Sami A. Al-Harbi, Yaaser Q. Almulaiky
Summary: The immobilization of beta-galactosidase on Cu-trimesic acid-based metal organic frameworks (Cu-TMAs) was studied. The immobilized enzyme showed good retention of activity, storage stability, and improved lactose hydrolysis activity. This study demonstrated the potential of Cu-TMAs as supports for immobilizing beta-galactosidase and suggested practical applications in various industrial processes.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Microbiology
Kalathinathan K. Pooja, Sankaranarayanan Gomathinayagam, Kodiveri M. Gothandam
Summary: The genome of Paracoccus marcusii KGP, isolated from marine sediment collected from the coast of the Bay of Bengal, was sequenced using Oxford Nanopore sequencing technology. The genome contains genes for industrially relevant enzymes and secondary metabolites, with the beta-galactosidase showing transgalactosylation property. Comparison with the CGMCC strain revealed significant differences in the KGP genome, possibly indicating strain-specific genes.
CURRENT MICROBIOLOGY
(2021)
Article
Food Science & Technology
Zhixin Wang, Jingjie Qi, Julie M. Goddard
Summary: The addition of trehalose, glucose, and sucrose was found to improve the stability of lactase under certain environmental conditions, prolonging its activity retention time. This enhanced stability opens up new possibilities for the application of lactase in bioprocessing.
INTERNATIONAL DAIRY JOURNAL
(2021)
Review
Food Science & Technology
Patrick Schulz, Syed S. H. Rizvi
Summary: The lactose-free dairy market has grown significantly in recent years, with lactose hydrolysis being the most commonly used method due to its economic and sustainable advantages. Current research mainly focuses on enzymatic hydrolysis methods and developing new commercially valuable products.
FOOD REVIEWS INTERNATIONAL
(2023)
Article
Agricultural Engineering
Nicolas Ramirez, Claudia Ubilla, Javiera Campos, Francisca Valencia, Carla Aburto, Carlos Vera, Andres Illanes, Cecilia Guerrero
Summary: The study evaluated the effects of operational variables on lactulose production by enzymatic transgalactosylation in fed-batch and repeated fed-batch. Feed flowrate and fructose to lactose molar ratio were the most significant variables affecting lactulose yield, productivity, and selectivity. The repeated fed-batch operation increased efficiency of biocatalyst use and accumulated productivity significantly compared to batch and fed-batch operations with the same biocatalyst.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Yuchen Li, Svitlana Miros, Hossein Kiani, Hans-Georg Eckhardt, Alfonso Blanco, Shane Mulcahy, Hugh McDonnell, Brijesh Kumar Tiwari, Ronald Halim
Summary: This study investigated the mechanism of lactose assimilation in Nannochloropsis oceanica for dairy-wastewater bioremediation and co-production of valuable feed/food ingredients. It was found that mixotrophic cultivation is necessary for lactose assimilation in N. oceanica, and the microalgae can produce beta-galactosidase enzyme to break down lactose. Under mixotrophic conditions, the microalgae can maintain dominance in the culture, while under heterotrophic conditions, bacteria take over the culture. These results have implications for onsite dairy wastewater treatment.
JOURNAL OF APPLIED PHYCOLOGY
(2023)
Article
Chemistry, Analytical
Xiaobin Zhou, Mengjun Wang, Junyang Chen, Xingguang Su
Summary: In this study, a high-performance nanozyme, Cu/NC NS, was designed and synthesized for the construction of a high-sensitivity biosensor platform. The Cu/NC NS exhibited high affinity towards H2O2 and TMB under acidic condition, allowing for the accurate detection of lactose and β-Gal through a multienzyme cascade colorimetric sensing system.
Article
Agriculture, Multidisciplinary
Belay Tilahun Tadesse, Ge Zhao, Paul Kempen, Christian Solem
Summary: This study discovered that a mutated strain of Streptococcus thermophilus can efficiently hydrolyze lactose using whole-cell lactase catalysts. Genome sequencing revealed a mutation that affects the growth of the strain and increases its lactose hydrolyzing ability. These findings could potentially lead to the production of sweet lactose-reduced yoghurt without the need for expensive purified lactase enzymes.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Food Science & Technology
Shuyue Luan, Xuguo Duan
Summary: In this study, a novel thermostable β-galactosidase (lacZBa) from Bacillus aryabhattai GEL-09 was investigated and found to have potential applications in the dairy industry. LacZBa exhibited maximum activity at 45°C and pH 6.0, and could hydrolyze lactose and oNPG. It efficiently recognized and catalyzed lactose, and could completely hydrolyze lactose in lactose solution and commercial milk within 4 hours at 45°C.
Article
Food Science & Technology
T. Inanan
Summary: This study aimed to optimize lactase immobilization onto Fe-chelated cryogel disks, and found that the immobilized lactase showed higher catalytic activity at different pH and temperature conditions, making it suitable for lactose-free milk preparation.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Jingjing Sun, Congyu Yao, Yujie Li, Wei Wang, Jianhua Hao, Yi Yu
Summary: In this study, a novel β-galactosidase was isolated from a deep-sea metagenome, overexpressed in Escherichia coli, and purified. The enzyme showed high activity at specific temperatures and pH conditions, and exhibited resistance to salt. Moreover, it displayed hydrolysis and transglycosylation activity towards substrates such as oNPG and lactose.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Carlos Vera, Cecilia Guerrero, Carla Aburto, Andres Cordova, Andres Illanes
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2020)
Article
Biochemistry & Molecular Biology
Cecilia Guerrero, Carla Aburto, Sebastian Suarez, Carlos Vera, Andres Illanes
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2020)
Article
Food Science & Technology
Carla Aburto, Cecilia Guerrero, Carlos Vera, Andres Illanes
LWT-FOOD SCIENCE AND TECHNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Cecilia Guerrero, Sebastian Suarez, Carla Aburto, Claudia Ubilla, Nicolas Ramirez, Carlos Vera, Andres Illanes
PROCESS BIOCHEMISTRY
(2020)
Article
Biotechnology & Applied Microbiology
Diego Ahumada, Felipe Arenas, Fabian Martinez-Gomez, Cecilia Guerrero, Andres Illanes, Carlos Vera
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2020)
Review
Food Science & Technology
Jessica Lopez, Carlos Vera, Ruben Bustos, Jennyfer Florez-Mendez
Summary: The use of native berries in Chile has been on the rise due to their high levels of bioactive compounds, particularly phenolic compounds like anthocyanins which have been shown to have potential health benefits such as protecting against cancer and cardiovascular diseases. This study reviews the nutritional aspects, phytochemical composition, and biological activities of native berries in Chile, emphasizing their importance in promoting healthy diets.
JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION
(2021)
Article
Biochemistry & Molecular Biology
Marcela Serey, Carlos Vera, Cecilia Guerrero, Andres Illanes
Summary: Aspergillus oryzae beta-galactosidase was immobilized in in-house quaternary ammonium agarose (QAA) for the first time to synthesis lactulose, resulting in a biocatalyst with high specific activity. The immobilized enzyme showed higher yield and specific productivity in batch reactions, and the support could be reused without compromising yield, productivity, and selectivity, reducing economic impact.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Food Science & Technology
Carlos Vera, Andres Illanes, Cecilia Guerrero
Summary: Prebiotics, such as non-digestible oligosaccharides (NDOs), are health promoting functional food ingredients that are extracted from natural sources or enzymatically synthesized. Enzymatic production of NDOs has become the preferred choice at large scale due to the complexity of chemical synthesis, with impressive advances in enzyme biocatalysis allowing for their production under harsh conditions.
CURRENT OPINION IN FOOD SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Luigi Tavernini, Oscar Romero, Carla Aburto, Fernando Lopez-Gallego, Andres Illanes, Lorena Wilson
Summary: Hybrid bioinorganic biocatalysts have drawn much attention for their simple synthesis, high efficiency, and structural features that enhance enzyme activity and stability. In this study, a biomineralization strategy was utilized to form hybrid nanocrystals from beta-galactosidase, showing high specific activity and remarkable thermal stability. This new biocompatible and inexpensive nanobiocatalyst demonstrated promising industrial applications with high enzyme recovery and stability.
Article
Agricultural Engineering
Nicolas Ramirez, Claudia Ubilla, Javiera Campos, Francisca Valencia, Carla Aburto, Carlos Vera, Andres Illanes, Cecilia Guerrero
Summary: The study evaluated the effects of operational variables on lactulose production by enzymatic transgalactosylation in fed-batch and repeated fed-batch. Feed flowrate and fructose to lactose molar ratio were the most significant variables affecting lactulose yield, productivity, and selectivity. The repeated fed-batch operation increased efficiency of biocatalyst use and accumulated productivity significantly compared to batch and fed-batch operations with the same biocatalyst.
BIORESOURCE TECHNOLOGY
(2021)
Article
Agricultural Engineering
Kevin Munoz, Diego Ahumada, Felipe Arenas, Cecilia Guerrero, Andres Illanes, Carlos Vera
Summary: The study found that butyl-beta-galactoside is significantly more soluble in the organic phase than lactose and monosaccharides. Additionally, higher values of the 1-butanol/aqueous phase ratio were shown to reduce secondary hydrolysis and increase yields. Interfacial properties for butyl-beta-galactoside were also determined at 25 degrees C.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biology
P. Munoz-Diaz, K. Jimenez, R. Luraschi, F. Cornejo, M. Figueroa, C. Vera, A. Rivas-Pardo, J. M. Sandoval, C. Vasquez, F. Arenas
Summary: In vivo reduction of tellurite is related to the concentration of total RSH within the cell, with a higher availability of cellular RSH leading to more significant tellurite reduction. It was observed that decreased resistance to tellurite in bacteria results in reduced tellurite reduction, impacting growth fitness. These results suggest a relationship between tellurite reduction, intracellular RSH content, and oxyanion bacterial resistance in an oxygen-independent anaerobic process.
BIOLOGICAL RESEARCH
(2022)
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)