Article
Multidisciplinary Sciences
Sandra Camarero-Espinosa, Lorenzo Moroni
Summary: This study presents a dynamic Janus scaffolds fabrication method that can be activated through external application of ultrasound, promoting cell proliferation, matrix deposition and osteogenic differentiation.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Nasrin Fazeli, Ehsan Arefian, Shiva Irani, Abdolreza Ardeshirylajimi, Ehsan Seyedjafari
Summary: This study provided a comparative investigation on the ability of 3D printed polycaprolactone (PCL) scaffolds and PCL-modified with hydroxyapatite (HA) and bioglasses (BG) scaffolds in calvarial bone regeneration. The results demonstrated that PCL scaffolds with HA and BG bioceramics have great potential applications.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Multidisciplinary
Han Liu, Lin Qiu, Hao Liu, Fengji Li, Yaru Fan, Lulu Meng, Xiaoqian Sun, Chaojun Zhan, Rui Luo, Chao Wang, Jun Zhang, Ruixin Li
Summary: This study utilized low-temperature 3D printing technology to construct a silk fibroin/collagen/hydroxyapatite composite scaffold, designed with different fiber cross-angle structures and subjected to various performance tests and cellular experiments. The results indicated that the 90 degrees fiber cross-angle structure significantly enhanced the mechanical properties of the scaffold, promoting cell proliferation and osteogenesis, providing insights into scaffold properties and applications.
Article
Polymer Science
Mauro Petretta, Alessandro Gambardella, Giovanna Desando, Carola Cavallo, Isabella Bartolotti, Tatiana Shelyakova, Vitaly Goranov, Marco Brucale, Valentin Alek Dediu, Milena Fini, Brunella Grigolo
Summary: The study developed magnetically assisted PCL-HAp-1% SPION scaffolds, showing promising results in terms of cell proliferation and osteogenic potential, indicating the potential for further in vitro and in vivo studies and validations.
Article
Engineering, Biomedical
Zhen Yang, Fuyang Cao, Hao Li, Songlin He, Tianyuan Zhao, Haoyuan Deng, Jianwei Li, Zhiqiang Sun, Chunxiang Hao, Jianzhong Xu, Quanyi Guo, Shuyun Liu, Weimin Guo
Summary: Microfracture is a commonly used surgical technique for cartilage defects, but its effectiveness remains questionable. This study explores the deficiency of microfractures in cartilage repair and proposes a therapeutic strategy using microenvironmentally optimized scaffolds to direct mesenchymal stem cell fate and improve cartilage regeneration.
ACTA BIOMATERIALIA
(2022)
Article
Biochemistry & Molecular Biology
Matteo Gasparotto, Pietro Bellet, Giorgia Scapin, Rebecca Busetto, Chiara Rampazzo, Libero Vitiello, Dhvanit Indravadan Shah, Francesco Filippini
Summary: This study developed a reliable and economical 3D-printed scaffold for tissue engineering applications. The results demonstrate that the scaffold's micro-topography and graphene properties synergistically control cell alignment and differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Multidisciplinary
Wenling Gao, Jintao Deng, Jianhua Ren, Wenhui Zhang, Zhe Wang, Ronghan He, Kun Wang, Xuetao Shi, Tangzhao Liang
Summary: This study investigates the effects of 3D hydroxyapatite scaffolds loaded with exosomes derived from BMSCs cultured under hypoxic or normoxic conditions on bone repair and regeneration. The results demonstrate that the 3D/H-exos enhance angiogenesis and osteogenesis, suggesting a promising approach for bone defect repair and reconstruction.
COMPOSITES PART B-ENGINEERING
(2022)
Article
Materials Science, Biomaterials
Paula Camacho, Anne Behre, Matthew Fainor, Kelly B. Seims, Lesley W. Chow
Summary: Functional repair of osteochondral tissue requires strategies to restore the spatial composition and organization found in native tissue, with peptide-functionalized polymers in 3D printing enabling unprecedented control of surface peptide presentation. The multi-peptide organization significantly influenced human mesenchymal stem cell response, driving differentiation towards specific cell types in a spatially controlled manner. This platform has the potential to fabricate biomaterials with spatially controlled biochemical cues for functional tissue regeneration without the need for differentiation factors.
BIOMATERIALS SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Dongyuan Luo, Boying Chen, Yu Chen
Summary: The currently available biomaterials for alveolar cleft repair have poor mechanical properties and biocompatibility. In this study, a 3D printed biomimetic scaffold was developed using a mixture of beta-tricalcium phosphate (beta-TCP) and bone marrow mesenchymal stem cells (BMSCs). The hybrid scaffold showed good biocompatibility and mechanical properties, and supported the attachment, proliferation, survival, differentiation, and osteogenic induction of BMSCs for alveolar cleft reconstruction.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Marcin Szustak, Edyta Gendaszewska-Darmach
Summary: Nanocellulose is a promising biocompatible biomaterial with good mechanical properties for use as a scaffold in cartilage tissue engineering. The limited regenerative capacity of cartilage due to its lack of vascularization and sparsely distributed chondrocytes poses challenges for cell infiltration, making nanocellulose scaffolds a potential solution. The scaffolds have been shown to induce stem cell differentiation into chondrocyte phenotypes, offering opportunities for cartilage tissue development.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Orthopedics
Muhammet Sefa Izgordu, Evren Isa Uzgur, Songul Ulag, Ali Sahin, Betul Karademir Yilmaz, Beyhan Kilic, Nazmi Ekren, Faik Nuzhet Oktar, Oguzhan Gunduz
Summary: This study aimed to evaluate the mechanical and biological performance of cartilage-like constructs produced by 3D printing. Different materials were used and combined in various ratios to fabricate the constructs, leading to differences in mechanical properties and biocompatibility of the resulting materials.
Article
Chemistry, Multidisciplinary
Silvia Cometta, Robert T. Jones, Alfredo Juarez-Saldivar, Bogdan C. Donose, Muhammad Yasir, Nathalie Bock, Tim R. Dargaville, Karl Bertling, Michael Bruenig, Aleksandar D. Rakic, Mark Willcox, Dietmar W. Hutmacher
Summary: The study demonstrates that immobilizing melimine on mPCL scaffolds can effectively prevent bacterial infections on implant surfaces and inhibit biofilm formation.
Article
Chemistry, Physical
Suji Choi, Keel Yong Lee, Sean L. Kim, Luke A. MacQueen, Huibin Chang, John F. Zimmerman, Qianru Jin, Michael M. Peters, Herdeline Ann M. Ardona, Xujie Liu, Ann-Caroline Heiler, Rudy Gabardi, Collin Richardson, William T. Pu, Andreas R. Bausch, Kevin Kit Parker
Summary: A hydrogel ink containing prefabricated gelatin fibers was developed to print organ-level scaffolds that mimic the organization of the heart. The addition of gelatin fibers allowed for controlled printing of free-standing 3D structures without additional support. The aligned fibers promoted the self-organization of human cardiomyocytes into anisotropic muscular tissues.
Article
Materials Science, Multidisciplinary
Gi Hoon Yang, MyungGu Yeo, Eunjeong Choi, Donggu Kang, Minseong Kim, Yunjoo Nam, So-Jung Gwak, Hye Hyun Yoo, Min-Jeong Park, Bongsu Jung, Woonhyeok Jeong, Hojun Jeon
Summary: The study found that different sizes of silica particles have varying effects on the physical and biological properties of polycaprolactone scaffolds. Particles with a size of 800 nm can improve the physical properties of the scaffold, while particles with a size of 100 nm can significantly enhance the biological properties of the scaffold.
MATERIALS & DESIGN
(2021)
Article
Engineering, Biomedical
Stephanie M. Kronstadt, Divya B. Patel, Louis J. Born, Daniel Levy, Max J. Lerman, Bhushan Mahadik, Shannon T. McLoughlin, Arafat Fasuyi, Lauren Fowlkes, Lauren Hoorens Van Heyningen, Amaya Aranda, Sanaz Nourmohammadi Abadchi, Kai-Hua Chang, Angela Ting Wei Hsu, Sameer Bengali, John W. Harmon, John P. Fisher, Steven M. Jay
Summary: This study evaluates the effects of a 3D-printed scaffold-perfusion bioreactor system on the production and bioactivity of extracellular vesicles (EVs) secreted from bone marrow-derived mesenchymal stem cells (MSCs). The results show that perfusion bioreactor culture significantly increases MSC EV production compared to conventional cell culture, and the MSC EVs generated using this system greatly improve wound healing in a diabetic mouse model. This study provides a promising solution to enhance the production and therapeutic efficacy of EVs.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Materials Science, Paper & Wood
Sandra Rodriguez-Fabia, Jonathan Torstensen, Lars Johansson, Kristin Syverud
Summary: This review provides an overview of the hydrophobization of lignocellulosic materials by polymer adsorption and grafting. Radiation-induced grafting is the most environmentally friendly technique, while controlled radical polymerization allows for the synthesis of polymers with complex structures. The choice of modification technique depends on the end-use of the material.
Review
Materials Science, Paper & Wood
Sandra Rodriguez-Fabia, Jonathan Torstensen, Lars Johansson, Kristin Syverud
Summary: This review summarizes the recent advances in the chemical modification of lignocellulosic materials to increase their hydrophobicity. The methods described in the review can result in changes in the materials' hydrophilicity, ranging from a slight decrease to superhydrophobic properties.
Article
Materials Science, Paper & Wood
Amalie Solberg, Jennifer Zehner, Ferdinand Somorowsky, Klaus Rose, Antti Korpela, Kristin Syverud
Summary: Nanopaper, a renewable and lightweight material, offers a promising alternative to plastics. However, its poor water resistance can be improved by coating it with ORMOCER®. The coated nanopaper not only exhibits excellent water resistance but also shows a distinct two-layer morphology.
Article
Multidisciplinary Sciences
Jonathan Torstensen, Vegar Ottesen, Sandra Rodriguez-Fabia, Kristin Syverud, Lars Johansson, Anders Lervik
Summary: We investigated the sorption of water to cellulose and found that it caused swelling of cellulose nanofibril (CNF) and cellulose nanocrystal (CNC) films. Molecular modeling showed that water sorbed to cellulose had decreased diffusion compared to bulk water, and the diffusion of sorbed water was less dependent on swelling temperature. This study provides new insights into the behavior of water sorption in cellulose materials.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Jost Ruwoldt, Mihaela Tanase-Opedal, Kristin Syverud
Summary: In this article, the authors explored solvents with lower harmfulness for UV spectrophotometry of lignin. They measured the absorptivity of lignin samples in different solvents and compared their purity. They also determined the Hansen solubility parameter of technical lignins and found that solvents with similar HSP had higher absorptivity. They identified ethylene glycol and a mixture of propylene carbonate, water, and ethanol as suitable solvents for lignin analysis. The authors compared different methods for determining phenolic hydroxyl units and found discrepancies between procedures and solvents. They concluded that several solvents were less harmful and important to consider when conducting UV spectrophotometry of lignin.
Article
Materials Science, Paper & Wood
Bastien Michel, Ellinor B. Heggset, Cecile Sillard, Kristin Syverud, Alain Dufresne, Julien Bras
Summary: Encapsulation of active principal ingredient (API) onto TEMPO-oxidized cellulose nanofibrils (CNFs) is possible, but challenges such as burst release and low water-solubility of API need to be addressed. This study evaluates the release property and antibacterial activity of CNF/beta-Cyclodextrin (beta-CD)/SD films. Results show that the CNF network structure helps reduce burst release, and the use of beta-CDs has a beneficial impact on antibacterial activity. These materials have potential for biomedical applications like wound dressing.
Article
Cell & Tissue Engineering
Pauline Cheng, Ahmad Rashad, Ankit Gangrade, Natan Roberto de Barros, Ali Khademhosseini, Jonathan Tam, Padmini Varadarajan, Devendra K. Agrawal, Finosh G. Thankam
Summary: Myocardial infarction leads to the loss of cardiomyocytes, but stem cell therapy shows promise in restoring cardiac function. This article discusses the application of different stem cell phenotypes and strategies to promote their differentiation into cardiomyocytes.
TISSUE ENGINEERING PART B-REVIEWS
(2023)
Article
Chemistry, Multidisciplinary
Reihaneh Haghniaz, Ankit Gangrade, Hossein Montazerian, Fahimeh Zarei, Menekse Ermis, Zijie Li, Yuxuan Du, Safoora Khosravi, Natan Roberto de Barros, Kalpana Mandal, Ahmad Rashad, Fatemeh Zehtabi, Jinghang Li, Mehmet R. Dokmeci, Han-Jun Kim, Ali Khademhosseini, Yangzhi Zhu
Summary: Developing theranostic devices to detect and control bleeding in the prehospital setting is an unmet medical need. This study presents an all-in-one theranostic platform that can diagnose local bleeding and stop hemorrhage by sandwiching silk fibroin (SF) between two silver nanowire (AgNW) based electrodes. The device shows excellent hemostatic properties, can differentiate between different body fluids, and has anti-infection efficiency. In addition, it is biocompatible and degrades in vivo without causing major inflammatory responses. Moreover, the theranostic platform demonstrates comparable hemostatic efficacy to a commercial hemostat in rat liver bleeding models, offering an unexplored strategy for intelligent hemorrhage management.
Article
Nanoscience & Nanotechnology
Ahmad Rashad, Martha Grondahl, Ellinor Baevre Heggset, Kamal Mustafa, Kristin Syverud
Summary: Cellulose nanofibrils (CNFs) are hydrophilic biocompatible polysaccharide materials derived from wood and plants. The presence of aldehyde groups on the surface of TO-CNF has little or no effect on MSCs attachment, proliferation, and osteogenic differentiation. TO-R-CNF samples, which lack aldehyde groups, stimulate cells in osteogenic medium to have higher alkaline phosphatase activity and form more biomineralization.
ACS APPLIED BIO MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Fatemeh Zehtabi, Ankit Gangrade, Kaylee Tseng, Reihaneh Haghniaz, Reza Abbasgholizadeh, Hossein Montazerian, Danial Khorsandi, Jamal Bahari, Amir Ahari, Neda Mohaghegh, Negar Hosseinzadeh Kouchehbaghi, Kalpana Mandal, Marvin Mecwan, Ahmad Rashad, Natan Roberto de Barros, Youngjoo Byun, Menekse Ermis, Han-Jun Kim, Ali Khademhosseini
Summary: This study incorporates doxycycline (DOX) into a shear-thinning biomaterial (STB)-based hydrogel to improve the embolizing efficacy of abdominal aortic aneurysms (AAAs). The addition of DOX inhibits MMP activity and promotes endothelial removal, preventing endoleaks. The STB-DOX hydrogel shows promising results in terms of injectability, strength, and aneurysm sac embolization.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Multidisciplinary Sciences
Daniel W. Hagey, Miina Ojansivu, Beklem R. Bostancioglu, Osama Saher, Jeremy P. Bost, Manuela O. Gustafsson, Roberto Gramignoli, Mathias Svahn, Dhanu Gupta, Molly M. Stevens, Andre Goergens, Samir EL Andaloussi
Summary: This study provides important insights into the cellular response to extracellular vesicles (EVs), showing that EV dose has a more significant effect than cell source. At high doses, exocytosis is down-regulated and lysosomal activity is up-regulated. However, at low doses, specific responses based on EV cell source are observed, reflecting the activities of the EV's source cells. Furthermore, immune cell-derived EVs are most associated with recipient cells.
Meeting Abstract
Cell & Tissue Engineering
Jannika Korkeamaki, Ahmad Rashad, Jennika Karvinen, Minna Kellomaki, Susanna Miettinen, Kamal Mustafa
TISSUE ENGINEERING PART A
(2023)
Review
Pharmacology & Pharmacy
Natan Roberto de Barros, Canran Wang, Surjendu Maity, Arne Peirsman, Rohollah Nasiri, Anna Herland, Menekse Ermis, Satoru Kawakita, Bruna Gregatti Carvalho, Negar Hosseinzadeh Kouchehbaghi, Rondinelli Donizetti Herculano, Zuzana Tirpakova, Seyed Mohammad Hossein Dabiri, Jean Lucas Tanaka, Natashya Falcone, Auveen Choroomi, Runrun Chen, Shuyi Huang, Elisheva Zisblatt, Yixuan Huang, Ahmad Rashad, Danial Khorsandi, Ankit Gangrade, Leon Voskanian, Yangzhi Zhu, Bingbing Li, Mohsen Akbarij, Junmin Leem, Mehmet Remzi Dokmeci, Han-Jun Kim, Ali Khademhosseini
Summary: This review discusses the latest developments in organoid engineering for biomedical applications, including improvements in engineering organoids and their applications in drug development and disease modeling. It also highlights the tools and techniques used to study organoids and their microenvironments, as well as the analysis of clinical trials and patents related to organoids, showing their potential for future personalized medicine. The challenges and future perspectives of using organoids to advance biomedical research are also discussed.
ADVANCED DRUG DELIVERY REVIEWS
(2023)
