Article
Cell Biology
Naomi C. Buntsma, Aleksandra Gasecka, Yvo B. W. E. M. Roos, Ton G. van Leeuwen, Edwin van der Pol, Rienk Nieuwland
Summary: In this study, we found that EDTA improves the stability of plasma EVs and is more effective at inhibiting the release of platelet EVs compared to citrate when platelets are activated.
Article
Chemistry, Analytical
Xiaowei Wei, Lijun Cai, Hanxu Chen, Luoran Shang, Yuanjin Zhao, Weijian Sun
Summary: This study proposes a novel system for multiplexed analysis of bladder cancer-derived urine exosomes using magnetic microspheres as barcoded microcarriers. The unique structure of the microcarriers allows for controllable movement and high sensitivity detection of exosomes. The noninvasive nature of the platform, combined with its good sensitivity, specificity, low sample consumption, and ease of use, indicates its great potential for bladder cancer diagnosis in clinical applications.
ANALYTICAL CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Limei Xu, Yujie Liang, Xiao Xu, Jiang Xia, Caining Wen, Peng Zhang, Li Duan
Summary: Extracellular vesicles derived from blood cells serve as important diagnostic biomarkers and potential drug delivery vehicles, with erythrocyte-derived vesicles being particularly promising for therapeutic interventions. Blood-derived EVs are ideal nanoplatforms for disease diagnosis and therapy.
Review
Biochemistry & Molecular Biology
Ainhoa Alberro, Leire Iparraguirre, Adelaide Fernandes, David Otaegui
Summary: Extracellular vesicles (EVs) play a vital role in intercellular communication, transferring information between nearby or distant cells. Blood is a major source of EVs, containing various types of EVs. Blood-derived EVs have significant potential in disease diagnosis, treatment monitoring, and prognosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Tania Soares Martins, Rui Marcalo, Cristovao B. da Cruz e Silva, Dario Trindade, Jose Catita, Francisco Amado, Tania Melo, Ilka Martins Rosa, Jonathan Vogelgsang, Jens Wiltfang, Odete A. B. da Cruz e Silva, Ana Gabriela Henriques
Summary: Exosomes in blood can serve as biomarkers for the diagnosis of Alzheimer's disease (AD) and monitoring disease progression. Proteomic analysis identified 9 significantly different abundant proteins between healthy controls and AD cases, and their levels were validated using antibody-based approaches. These findings contribute to the development of blood-based tools for AD diagnosis.
MOLECULAR NEUROBIOLOGY
(2022)
Review
Geriatrics & Gerontology
Naushad Ahmad Khan, Mohammad Asim, Ayman El-Menyar, Kabir. H. H. Biswas, Sandro Rizoli, Hassan Al-Thani
Summary: Developing effective disease-modifying therapies for neurodegenerative diseases requires reliable diagnostic and progression indicators. Extracellular vesicles (EVs) play an important role in intercellular communication and transport of cell-specific cargos. EVs may have implications in traumatic brain injury (TBI) and other neurodegenerative diseases. EVs have therapeutic potential and can represent intracellular processes. EV-based biomarkers for TBI are more reflective of cellular damage and neuroinflammation.
FRONTIERS IN AGING NEUROSCIENCE
(2022)
Article
Oncology
Melike Mut, Zelal Adiguzel, Canan Cakir-Aktas, Sahin Hanalioglu, Gamze Gungor-Topcu, Ezgi Kiyga, Ilkay Isikay, Aydan Sarac, Figen Soylemezoglu, Thomas Strobel, Elisabet Ampudia-Mesias, Charles Cameron, Tulay Aslan, Eray Tekirdas, Mutlu Hayran, Kader Karli Oguz, Christine Henzler, Nurten Saydam, Okay Saydam
Summary: In this study, RNA sequencing of serum extracellular vesicles (EVs) was used to identify new tumor markers for glioblastoma. The results showed that serum-EV-based biomarker panels could accurately predict the status of various genetic mutations in glioblastoma, such as IDH1 mutation and MGMT promoter methylation. These biomarkers can be used for early cancer risk prediction and improved patient stratification and treatment strategies. This is the first study to demonstrate the diagnostic potential of serum-EV-based biomarker panels for glioblastoma.
Article
Oncology
Mariantonia Logozzi, Rossella Di Raimo, Davide Mizzoni, Stefano Fais
Summary: Antitumor therapy is considering the use of exosomes as a potential delivery system for anti-cancer molecules, aiming to improve efficacy and reduce toxicity. Although more research is needed, pre-clinical data supports the use of exosomes as nanodevices in cancer treatment. However, the lack of clinical data and the identification of the optimal cellular source of exosomes are important limitations.
SEMINARS IN CANCER BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Yu-Chen Jia, Yi-Xuan Ding, Wen-Tong Mei, Yu-Ting Wang, Zhi Zheng, Yuan-Xu Qu, Kuo Liang, Jia Li, Feng Cao, Fei Li
Summary: Comprehensive reviews and large population-based cohort studies have significantly reduced the incidence and mortality of pancreatitis. The study of extracellular vesicles (EVs), especially exosomes, in pancreatitis has shown potential for identifying cell-to-cell communication and influencing the disease's development and progression. Plasma exosomes of acute pancreatitis can reach the alveolar cavity and activate alveolar macrophages to cause acute lung injury, suggesting their use as biomarkers and therapeutic agents for this disease.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Rocio Munoz-Hernandez, Angela Rojas, Sheila Gato, Javier Gallego, Antonio Gil-Gomez, Maria Jose Castro, Javier Ampuero, Manuel Romero-Gomez
Summary: Extracellular vesicles (EVs) are membrane-derived vesicles released by various cell types, functioning as intercellular mediators of cell-cell communication. The development and optimization of methods for EV isolation are crucial for understanding their biological functions and potential therapeutic applications. This article comprehensively reviews the role of hepatic EVs in liver diseases and discusses their potential as 'liquid biopsies' for early diagnosis, follow-up, and prognosis, as well as their use in drug delivery systems for liver disease therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Caroline McLaughlin, Pallab Datta, Yogendra P. Singh, Alexis Lo, Summer Horchler, Irina A. Elcheva, Ibrahim T. Ozbolat, Dino J. Ravnic, Srinivas Koduru
Summary: Extracellular vesicles (EVs) are small lipid bilayer-delimited particles that transmit regulatory functions in the body. MSC-produced EVs have low immunogenicity and can be used as an alternative to cellular therapies.
Article
Biochemical Research Methods
Qinming Zhang, Hannah J. Loghry, Jingjing Qian, Michael J. Kimber, Liang Dong, Meng Lu
Summary: This paper introduces a unique exosome assay technology that combines magnetic nanoparticle-based exosome extraction and photonic crystal-based label-free exosome detection in a single vessel within one hour, with improved sensitivity and selectivity. The high specificity is achieved by functionalizing both nanoparticles and photonic crystals with specific antibodies, resulting in a low limit of detection and high enrichment factor for exosomes. The method is validated for analyzing membrane proteins of exosomes with high selectivity and sensitivity, showing great potential for exosome-based disease diagnostics.
Article
Biochemistry & Molecular Biology
Marco Marchisio, Pasquale Simeone, Giuseppina Bologna, Eva Ercolino, Laura Pierdomenico, Damiana Pieragostino, Alessia Ventrella, Francesca Antonini, Genny Del Zotto, Daniele Vergara, Christian Celia, Luisa Di Marzio, Piero Del Boccio, Antonella Fontana, Domenico Bosco, Sebastiano Miscia, Paola Lanuti
Summary: The study optimized a procedure using PFC to identify and separate circulating EVs from different cell origins, with the use of LCD staining being essential for PFC detection and sorting of EVs from fresh body fluids.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Engineering, Biomedical
Zichang Liu, Yu Zhuang, Lianfei Fang, Changyong Yuan, Xudong Wang, Kaili Lin
Summary: Osteoarthritis (OA) is a prevalent joint disease that lacks effective early diagnosis and treatment. Extracellular vesicles (EVs) have been found to play an important role in the progression of OA by influencing inflammation, metabolism, and aging. Researchers are exploring the potential of using EVs for the diagnosis and treatment of OA, but face challenges and limitations in their applications.
BIOACTIVE MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Mi Ho Jeong, Taehwang Son, Yoo Keung Tae, Chan Hee Park, Hee Seung Lee, Moon Jae Chung, Jeong Youp Park, Cesar M. M. Castro, Ralph Weissleder, Jung Hyun Jo, Seungmin Bang, Hyungsoon Im
Summary: Cholangiocarcinoma (CCA) is a fatal disease often diagnosed at advanced stages. There is a lack of effective diagnostic methods or biomarkers for early detection of CCA. A nanoplasmonic sensing technology called FLEX is developed for sensitive and robust single extracellular vesicle (EV) analysis, which detected a wide heterogeneity of CCA-derived EVs and identified tumor markers in clinical bile samples. The FLEX assay showed better diagnostic performance than current clinical markers, offering a potential tool for early CCA diagnosis.
Article
Chemistry, Multidisciplinary
Rasmus Munter, Camilla Stavnsbjerg, Esben Christensen, Mikkel E. Thomsen, Allan Stensballe, Anders E. Hansen, Ladan Parhamifar, Kasper Kristensen, Jens B. Simonsen, Jannik B. Larsen, Thomas L. Andresen
Summary: Coating nanoparticles with PEG does not effectively make the particles stealth to surface active proteins. A microscopy-based method is introduced to directly observe opsonization at the single nanoparticle level. The study reveals that different surface coatings have significant effects on opsonization on liposomes and that opsonization is heterogeneous, with both opsonized and non-opsonized liposomes co-existing. This assay provides mechanistic details about opsonization at the single nanoparticle level previously inaccessible to established bulk assays.
Article
Chemistry, Physical
Denis Selnihhin, Kim Mortensen, Jannik B. Larsen, Jens B. Simonsen, Finn Skou Pedersen
Summary: In this study, DNA-origami nanobeads were designed and constructed using DNA nanotechnology for fluorescence/antigen quantification in flow cytometry. The effectiveness of the bead design was verified through various microscopy techniques. The results demonstrate that DNA beads can accelerate quantitative studies of the biology of individual particles in flow cytometry.
Article
Cell Biology
Jaco Botha, Aase Handberg, Jens B. Simonsen
Summary: Flow cytometry is commonly used to characterize extracellular vesicles (EVs). However, this study found that PS labeling and detergent lysis methods may be confounded by lipoproteins. It suggests using transmembrane proteins to differentiate EVs from lipoproteins.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Article
Chemistry, Multidisciplinary
Xin Li, Holmfridur R. Halldorsdottir, Sven Weller, Anna Colliander, Martin Bak, Paul Kempen, Gael Clergeaud, Thomas L. Andresen
Summary: Although adoptive T cell therapy for cancer treatment has limitations, loading T cells with SHP099 can enhance therapeutic efficacy. Cell-loaded SHP099 enables sustained inhibition of the PD-1/PD-L1 signaling and increases cytolytic activity of the T cells. This is a promising therapeutic strategy.
Article
Chemistry, Multidisciplinary
Mia Danielsen, Casper Hempel, Thomas L. Andresen, Andrew J. Urquhart
Summary: Protein and peptide biopharmaceuticals have revolutionized the treatment of various diseases. Nanocarrier delivery systems offer a solution to overcome challenges such as rapid degradation, and protein and peptide nanoclusters have the potential to mimic the advantages of existing nanocarriers while eliminating issues such as low drug loading and potential side effects.
JOURNAL OF CONTROLLED RELEASE
(2022)
Review
Pharmacology & Pharmacy
Camilla Hald Albertsen, Jayesh A. Kulkarni, Dominik Witzigmann, Marianne Lind, Karsten Petersson, Jens B. Simonsen
Summary: The translation discusses the important role of lipid nanoparticles in mRNA vaccines against COVID-19, as well as the contributions of lipid components to the LNP delivery platform. Challenges and strategies related to LNPs in treating various diseases are also explored.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Article
Biotechnology & Applied Microbiology
Arjen Weller, Morten B. Hansen, Rodolphe Marie, Adam C. Hundahl, Casper Hempel, Paul J. Kempen, Henrik L. Frandsen, Ladan Parhamifar, Jannik B. Larsen, Thomas L. Andresen
Summary: The unsuccessful clinical translation of orally delivered biological drugs is a major challenge in pharmaceutical development, attributed to the insufficient understanding of intestinal drug transport mechanisms. In this study, a novel microfluidic platform was utilized to develop an in vitro intestinal epithelial barrier compatible with advanced imaging techniques, allowing direct tracking of drug transport across the barrier. This sophisticated imaging-based model provides valuable mechanistic insights for drug transport studies and has the potential to facilitate in vivo and clinical translation in drug development.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Carmen Radeke, Raphael Pons, Marko Mihajlovic, Jonas R. Knudsen, Sarkhan Butdayev, Paul J. Kempen, Charis-Patricia Segeritz, Thomas L. Andresen, Christian K. Pehmoller, Thomas E. Jensen, Johan U. Lind
Summary: In order to achieve the automated fabrication of complex tissue mimicking constructs through 3D bioprinting, it is necessary to develop bioinks that are not only printable and biocompatible but also have integrated cell-instructive properties. Here, we present a scalable technique for generating nanofiber 3D printing inks with unique tissue-guiding capabilities. By tailoring the size and dispersibility of cellulose fibrils, we are able to create nanofibers that match the size and dimensions of natural collagen fibers, allowing for the orientation of cells and the spatial organization of engineered tissues during printing.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Weiguang Jin, Xin Li, Gael Clergeaud, Roslyn M. Ray, Marie Karen Tracy Hong Lin, Thomas Lars Andresen, Charlotte Held Gotfredsen, Martin Nielsen, Kira Astakhova, Katrine Qvortrup
Summary: This study presents a novel strategy that utilizes metalloporphyrin cages to protect and prolong the half-lives of small RNAs (sRNAs). The cage binding also facilitates the internalization of sRNAs, and the released sRNAs maintain their genetic activity in an acidic intracellular environment. Moreover, the photodynamic properties of the cage enable efficient in vitro cell killing in breast cancer treatment.
CELL REPORTS PHYSICAL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Mia Danielsen, Paul Joseph Kempen, Thomas Lars Andresen, Andrew James Urquhart
Summary: The development of new and improved formulations for biopharmaceuticals is driven by the growing interest in this class of therapeutics and the challenges in formulation and delivery. Nanoclusters (NCs) are a type of formulation strategy where the biopharmaceutical is clustered reversibly to function as both the therapeutic and the vehicle. In this study, insulin NCs (INCs) were formulated using a new methodology of first crosslinking proteins followed by desolvation. The crosslinked INCs showed controlled synthesis, good biocompatibility, and slightly lower potency compared to the unmodified protein.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Nanoscience & Nanotechnology
Marina Simon, Jesper Tranekjaer Jorgensen, Kamilla Norregaard, Jonas Rosager Henriksen, Gael Clergeaud, Thomas L. Andresen, Anders Elias Hansen, Andreas Kjaer
Summary: Traditional cancer treatments are often insufficient to achieve complete responses, so combination therapies are commonly used. Nanoparticle-based photothermal therapy (PTT) is a promising add-on treatment that uses localized hyperthermia to kill cancer cells. In this study, we evaluated the combination of gold nanoshell-based PTT and liposomal doxorubicin for colorectal cancer treatment.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Chemistry, Multidisciplinary
Adam Coln Hundahl, Arjen Weller, Jannik Bruun Larsen, Claudia U. Hj, Morten B. Hansen, Ann-Kathrin Muendler, Astrid Knuhtsen, Kasper Kristensen, Eva C. Arnspang, Thomas Lars Andresen, Kim I. Mortensen, Rodolphe Marie
Summary: Oral drug delivery is the preferred administration route for most drugs, but it is limited for biologics due to the intestinal barrier. One strategy to improve the absorption is chemical modification through lipidation. However, the mechanistic understanding of the effect is largely unexplored. This study developed a method to quantify peptide transport through a monolayer of Caco-2 cells and investigated the effects of lipidation on transport mechanism.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Medicine, Research & Experimental
Rasmus Munter, Esben Christensen, Thomas L. Andresen, Jannik B. Larsen
Summary: Nucleic acid therapies are considered a game changer in medicine, but concerns exist regarding adverse reactions against lipid nanoparticles used for mRNA delivery. This research shows that the administration route can regulate anti-particle immunity, with intramuscular injections generating lower levels of anti-nanoparticle antibodies compared to intravenous and subcutaneous injections.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2023)
Article
Biology
Milena Vujovic, Paolo Marcatili, Benny Chain, Joseph Kaplinsky, Thomas Lars Andresen
Summary: The TCRDivER algorithm integrates clone size and sequence similarity of T cell receptors (TCR) to reveal greater complexity in TCR repertoires and novel TCR repertoire traits. This tool is important for monitoring disease or therapy progression. Rating: 8 out of 10.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Mikkel O. Norgard, Philip M. Lund, Nazmie Kalisi, Thomas L. Andresen, Jannik B. Larsen, Stefan Vogel, Per Svenningsen
Summary: Extracellular vesicle (EV) secretion rate is increased by hypoxia-induced reactive oxygen species (ROS) production via the mitochondrial electron transport chain (ETC) and hypoxia-induced factor (HIF)-1 signaling. Stimulation of ETC by dichloroacetic acid (DCA) significantly increases EV secretion, which can be blocked by the antioxidant TEMPO and rotenone, an inhibitor of Complex I in the ETC. Inhibition of Complex III using antimycin A or blocking the mevalonate pathway and tyrosine metabolites with pitavastatin and 4-nitrobenzoate, respectively, enhances ROS-dependent EV secretion. These findings demonstrate that hypoxia-mimetics targeting the ETC can modify EV secretion, and ROS produced by the ETC acts as a potent stimulus for EV secretion.
