Characterisation of sensor kinase by CD spectroscopy: golden rules and tips

Exploring the (Almost) Unknown: Archaeal Two-Component Systems

(2018) Tino Krell   JOURNAL OF BACTERIOLOGY

Cryo-electron microscopy of membrane proteins

(2018) Nopnithi Thonghin et al.   METHODS

Human mitochondrial pyruvate carrier 2 as an autonomous membrane transporter

(2018) Raghavendra Sashi Krishna Nagampalli et al.   Scientific Reports

A dual regulation mechanism of histidine kinase CheA identified by combining network-dynamics modeling and system-level input-output data

(2018) Bernardo A. Mello et al.   PLoS Computational Biology

UV-Denaturation Assay to Assess Protein Photostability and Ligand-Binding Interactions Using the High Photon Flux of Diamond B23 Beamline for SRCD

(2018) Rohanah Hussain et al.   MOLECULES

Mechanism of transmembrane signaling by sensor histidine kinases

(2017) Ivan Gushchin et al.   SCIENCE

Hydrodynamics of the VanA-type VanS histidine kinase: an extended solution conformation and first evidence for interactions with vancomycin

(2017) Mary K. Phillips-Jones et al.   Scientific Reports

Purification of bacterial membrane sensor kinases and biophysical methods for determination of their ligand and inhibitor interactions

(2016) R. Hussain et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

The Staphylococcus aureus AirSR Two-Component System Mediates Reactive Oxygen Species Resistance via Transcriptional Regulation of Staphyloxanthin Production

(2016) Jeffrey W. Hall et al.   INFECTION AND IMMUNITY

Insights into Protein–Ligand Interactions: Mechanisms, Models, and Methods

(2016) Xing Du et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

The changing landscape of membrane protein structural biology through developments in electron microscopy

(2016) Shaun Rawson et al.   MOLECULAR MEMBRANE BIOLOGY

Increased duplex stabilization in porphyrin-LNA zipper arrays with structure dependent exciton coupling

(2016) Daniel G. Singleton et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

High-throughput SRCD using multi-well plates and its applications

(2016) Rohanah Hussain et al.   Scientific Reports

Application of circular dichroism and magnetic circular dichroism for assessing biopharmaceuticals formulations photo-stability and small ligands binding properties

(2015) Edoardo Longo et al.   INTERNATIONAL JOURNAL OF PHARMACEUTICS

Accurate secondary structure prediction and fold recognition for circular dichroism spectroscopy

(2015) András Micsonai et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

CryoEM and computer simulations reveal a novel kinase conformational switch in bacterial chemotaxis signaling

(2015) C Keith Cassidy et al.   eLife

CDApps: integrated software for experimental planning and data processing at beamline B23, Diamond Light Source

(2015) Rohanah Hussain et al.   JOURNAL OF SYNCHROTRON RADIATION

TROSY NMR with a 52 kDa sugar transport protein and the binding of a small-molecule inhibitor

(2014) Arnout P. Kalverda et al.   MOLECULAR MEMBRANE BIOLOGY

Visualizing autophosphorylation in histidine kinases

(2014) Patricia Casino et al.   Nature Communications

Using UV-absorbance of intrinsic dithiothreitol (DTT) during RP-HPLC as a measure of experimental redox potential in vitro

(2013) Angie Seo et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

The 3.2 Å Resolution Structure of a Receptor:CheA:CheW Signaling Complex Defines Overlapping Binding Sites and Key Residue Interactions within Bacterial Chemosensory Arrays

(2013) Xiaoxiao Li et al.   BIOCHEMISTRY

Isothermal titration calorimetry of membrane proteins — Progress and challenges

(2013) Krishna Rajarathnam et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Autophosphorylation Activity of a Soluble Hexameric Histidine Kinase Correlates with the Shift in Protein Conformational Equilibrium

(2013) Marta Wojnowska et al.   CHEMISTRY & BIOLOGY

Functional Characterization of SbmA, a Bacterial Inner Membrane Transporter Required for Importing the Antimicrobial Peptide Bac7(1-35)

(2013) G. Runti et al.   JOURNAL OF BACTERIOLOGY

Transcriptomic and biochemical analyses identify a family of chlorhexidine efflux proteins

(2013) K. A. Hassan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Full-Length Structure of a Sensor Histidine Kinase Pinpoints Coaxial Coiled Coils as Signal Transducers and Modulators

(2013) Ralph P. Diensthuber et al.   STRUCTURE

Membrane Protein Structure Determination Using Crystallography and Lipidic Mesophases: Recent Advances and Successes

(2012) Martin Caffrey et al.   BIOCHEMISTRY

Interactions of the intact FsrC membrane histidine kinase with its pheromone ligand GBAP revealed through synchrotron radiation circular dichroism

(2012) Simon G. Patching et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Revealing the Ligand Binding Site of NhaA Na+/H+Antiporter and Its pH Dependence

(2012) Michal Maes et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A systematic approach to the amplified expression, functional characterization and purification of inositol transporters fromBacillus subtilis

(2012) Kim E. Bettaney et al.   MOLECULAR MEMBRANE BIOLOGY

Thermodynamic studies of ligand binding to the human homopentameric glycine receptor using isothermal titration calorimetry

(2012) Annemarie Beate Wöhri et al.   MOLECULAR MEMBRANE BIOLOGY

Structure of the TatC core of the twin-arginine protein transport system

(2012) Sarah E. Rollauer et al.   NATURE

Interactions of the intact FsrC membrane histidine kinase with the tricyclic peptideinhibitor siamycin I revealed through synchrotron radiation circular dichroism

(2012) Mary K. Phillips-Jones et al.   PHYSICAL CHEMISTRY CHEMICAL PHYSICS

Anti-HIV siamycin I directly inhibits autophosphorylation activity of the bacterial FsrC quorum sensor and other ATP-dependent enzyme activities

(2011) Pikyee Ma et al.   FEBS LETTERS

Fluorescence quenching and ligand binding: A critical discussion of a popular methodology

(2011) Marco van de Weert et al.   JOURNAL OF MOLECULAR STRUCTURE

Circular dichroism beamline B23 at the Diamond Light Source

(2011) Rohanah Hussain et al.   JOURNAL OF SYNCHROTRON RADIATION

Role of two-component systems in the resistance ofStaphylococcus aureusto antibacterial agents

(2011) Miki Kawada-Matsuo et al.   Virulence

Solution- and solid-state NMR studies of GPCRs and their ligands

(2010) Satita Tapaneeyakorn et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Measuring circular dichroism in a capillary cell using the b23 synchrotron radiation CD beamline at diamond light source

(2010) Tamás Jávorfi et al.   CHIRALITY

Membrane domain structures of three classes of histidine kinase receptors by cell-free expression and rapid NMR analysis

(2010) I. Maslennikov et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

(2010) Martin Caffrey et al.   Jove-Journal of Visualized Experiments

Solid-state NMR for the analysis of high-affinity ligand/receptor interactions

(2009) P.T.F. Williamson   CONCEPTS IN MAGNETIC RESONANCE PART A

On the Mechanism of Membrane Protein Crystallization in Lipidic Mesophases†

(2008) Martin Caffrey   CRYSTAL GROWTH & DESIGN

Overcoming the challenges of membrane protein crystallography

(2008) Elisabeth P Carpenter et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Expression, purification and activities of the entire family of intact membrane sensor kinases fromEnterococcus faecalis

(2008) Pikyee Ma et al.   MOLECULAR MEMBRANE BIOLOGY

Large-scale expression and purification of the major HIV-1 coreceptor CCR5 and characterization of its interaction with RANTES

(2008) Lydia Nisius et al.   PROTEIN EXPRESSION AND PURIFICATION