Article
Plant Sciences
Hyeran Moon, A-Ram Jeong, Oh-Kyu Kwon, Chang-Jin Park
Summary: Orphan genes are commonly found in genomes, but their functions are largely unknown. In this study, a defense-related orphan gene Xio1 was identified and found to play an important role in rice's defense mechanism, providing a new resource for crop breeding.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Seungmin Son, Jong Hee Im, Giha Song, Suhyeon Nam, Sang Ryeol Park
Summary: The phytohormone abscisic acid (ABA) regulates the immune response of rice to Xanthomonas oryzae pv. oryzae (Xoo). This study identified OsWRKY114 as a negative regulator of ABA, reducing the susceptibility of rice to Xoo infection and affecting the expression of ABA-associated genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Multidisciplinary
Hongxia Lu, Zhongjie Shen, Yujun Xu, Linjing Wu, Deyu Hu, Runjiang Song, Baoan Song
Summary: This study found that ethylicin (ET) inhibited the proliferation of Xanthomonas oryzae pv. oryzae (Xoo) by increasing the content of defense enzymes and chlorophyll in rice. It was also discovered that ET activated the abscisic acid (ABA) signal pathway and made the protein kinase OsCPK24 more active, enhancing the resistance of rice to Xoo.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Virology
Mengju Liu, Ye Tian, Haitham E. M. Zaki, Temoor Ahmed, Rong Yao, Chengqi Yan, Sebastian Leptihn, Belinda Loh, Muhammad Shafiq Shahid, Fang Wang, Jianping Chen, Bin Li
Summary: This study aimed to investigate the co-evolution of bacterial pathogens with their host plants as well as with their bacterial viruses. Through experiments, several genes associated with both phage sensitivity and host pathogenicity were identified, and three genes were found to play a key role in bacterial virulence and phage resistance.
Article
Biochemistry & Molecular Biology
Xing Dai, Yankai Wang, Kaili Yu, Yonghui Zhao, Langyu Xiong, Ruozhong Wang, Shengben Li
Summary: The overexpression of OsNPR1 enhances rice resistance to Xanthomonas oryzae pv. oryzae (X-oo) by bidirectionally regulating gene expression in rice and X-oo. In X-oo-infected OsNPR1-OE plants, rice genes involved in cell wall biosynthesis, SA signaling pathways, and PR genes are upregulated, while X-oo genes involved in energy metabolism, oxidative phosphorylation, biosynthesis, and transportation are repressed. The downregulation of virulence genes, including components of type III and other secretion systems, is observed in X-oo due to OsNPR1 overexpression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Van Schepler-Luu, Coline Sciallano, Melissa Stiebner, Chonghui Ji, Gabriel Boulard, Amadou Diallo, Florence Auguy, Si Nian Char, Yugander Arra, Kyrylo Schenstnyi, Marcel Buchholzer, Eliza P. I. Loo, Atugonza L. Bilaro, David Lihepanyama, Mohammed Mkuya, Rosemary Murori, Ricardo Oliva, Sebastien Cunnac, Bing Yang, Boris Szurek, Wolf B. Frommer
Summary: Bacterial leaf blight (BB) of rice, caused by Xanthomonas oryzae pv. oryzae (Xoo), poses a threat to global food security and the livelihood of small-scale rice producers. Despite robust global rice trade, Xoo strains from different regions show complete continental segregation. Unprecedented BB outbreaks were discovered in Tanzania, caused by Xoo strains carrying Asian-type TAL effectors and iTAL effectors, unlike endemic African Xoo strains. In order to protect African rice production from this emerging threat, a hybrid CRISPR-Cas9/Cpf1 system was developed to edit TALe-binding elements in SWEET promoters. The edited lines displayed broad-spectrum resistance against Asian and African Xoo strains.
Article
Agronomy
Tabasia Amin, Vishal Gupta, Akash Sharma, Pardeep Kumar Rai, Vijay Kumar Razdan, Satish Kumar Sharma, Santosh Kumar Singh, Javeed Ahmad Lone, Munazah Yaqoob, Brajeshwar Singh, Sushil Kumar Gupta
Summary: Rice is a vital food source for over half of the global population. Bacterial leaf blight, caused by Xanthomonas oryzae pv. oryzae, is a highly destructive disease for rice in India. The study found diverse pathogenic variations of Xoo in the basmati-growing region of Jammu and Kashmir.
Article
Plant Sciences
Xiaolin Chen, Qili Li, Jinkai Wang, Yu Zhang, Lihua Tang, Suiping Huang, Jianyou Mo, Tangxun Guo
Summary: In this study, a hypervirulent strain C9-3 of Xanthomonas oryzae pv. oryzae, which can overcome several resistance genes in rice, was sequenced using single molecule real-time (SMRT) sequencing. The genome consists of a single circular chromosome with a size of 4,924,298 bp and contains 4,715 genes. Annotation and analysis revealed the presence of 17 transcription activator-like effectors, including typical and truncated TALEs, in the genome. This study provides important insights into the discovery of new virulence effectors and the understanding of rice-X. oryzae pv. oryzae interactions.
Article
Plant Sciences
Xinxiang A, Yayun Yang, Xifeng Chen, Cuifeng Tang, Feifei Zhang, Chao Dong, Bin Wang, Pengcheng Liu, Luyuan Dai
Summary: This study reports the complete genome sequence of a hypervirulent strain, YNCX, isolated from the high-altitude japonica rice-growing region in Yunnan Plateau. The genome sequence of YNCX provides a valuable resource for high-altitude races and enables the identification of new virulence TALE effectors, contributing to a better understanding of rice-Xoo interactions.
Article
Biochemistry & Molecular Biology
Yuxuan Hou, Yan Liang, Changdeng Yang, Zhijuan Ji, Yuxiang Zeng, Guanghao Li, E. Zhiguo
Summary: In 2020-2022, there was an outbreak of rice bacterial blight (BB) in the Yangtze River area, China. We determined the complete genome of Xanthomonas oryzae pv. oryzae (Xoo) strain LA20 using PacBio and Illumina sequencing. Comparative analysis of LA20 and the representative strain YC11 revealed significant variability in Tal genes, which may be responsible for LA20's ability to overcome resistance in rice varieties, leading to the resurgence of BB. The complete genome of this new pandemic Xoo strain will provide insights into pathogen evolution and the epidemic status of the disease in China.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Priya Lal Biswas, Ujjal Kumar Nath, Sharmistha Ghosal, Gayatri Goswami, Md Shalim Uddin, Omar M. Ali, Arafat Abdel Hamed Abdel Latef, Alison M. Laing, Yong-Ming Gao, Akbar Hossain
Summary: In this study, combinations of four BB resistance genes were pyramided into populations derived from a widely planted Indonesian rice variety crossed with an IRBB60 resistant variety. Promising recombinants with high yield potential and broad spectrum resistance were identified through marker-assisted selection. The developed BB-resistant RILs have the potential to be further developed for cultivation or as sources of BB resistance for varietal improvement in other rice lines.
Article
Microbiology
Mengjiao Liu, Ran Hu, Mian Xia, Xiaoqing He, Yi Jin
Summary: Bacterial leaf blight (BLB) and bacterial leaf streak (BLS), caused by Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc), can be effectively controlled using lytic broad-spectrum phages pXoo2106 and pXoo2107. These phages inhibit the growth of Xoo and Xoc and have strong biocontrol potential against BLB and BLS in field applications.
ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Plant Sciences
H. T. M. To, V. P. Nguyen, H. H. Chu, N. T. P. Mai
Summary: Research has shown that phosphate deficiency enhances rice resistance to bacterial leaf blight, and the activation of the jasmonic acid pathway depends on the rice cultivar. Pi-response genes are involved in phosphate stress and may participate in the regulation of overall plant growth under various abiotic stresses. These findings provide new insights into the interaction between phosphate deficiency and the jasmonic acid pathway and the subsequent effect on plant disease resistance.
BIOLOGIA PLANTARUM
(2023)
Article
Plant Sciences
Yaze Kong, Gang Wang, Xian Chen, Linying Li, Xueying Zhang, Sangtian Chen, Yuqing He, Gaojie Hong
Summary: Phosphate and MYC2-mediated JA pathway play crucial roles in plant growth and development. In rice, Pi starvation activates OsMYC2 signaling to enhance resistance to Xoo infection, with the direct regulation of OsPHR2 on OsMYC2 promoter confirmed. These findings highlight a molecular link between OsMYC2-mediated JA pathway and phosphate starvation signaling in monocot plants.
PLANT CELL AND ENVIRONMENT
(2021)
Review
Plant Sciences
Xiameng Xu, Ying Li, Zhengyin Xu, Jiali Yan, Yong Wang, Yijie Wang, Guanyun Cheng, Lifang Zou, Gongyou Chen
Summary: This article briefly describes rice resistance breeding in China, TALE properties and their roles, BB resistance (R) and susceptibility (S) genes in rice, the arms-race between TALEs and TALE-targets, and strategies for breeding disease-resistant crops.
PHYTOPATHOLOGY RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Jianbo Cao, Meng Zhang, Mengmeng Zhu, Limin He, Jinghua Xiao, Xianghua Li, Meng Yuan
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Multidisciplinary Sciences
Yinggen Ke, Meng Yuan, Hongbo Liu, Shugang Hui, Xiaofeng Qin, Jie Chen, Qinglu Zhang, Xianghua Li, Jinghua Xiao, Qifa Zhang, Shiping Wang
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Microbiology
Jianbo Cao, Chuanliang Chu, Meng Zhang, Limin He, Lihong Qin, Xianghua Li, Meng Yuan
Editorial Material
Plant Sciences
Jingjing Tian, Guoyong Xu, Meng Yuan
TRENDS IN PLANT SCIENCE
(2020)
Letter
Biotechnology & Applied Microbiology
Zhiming Feng, Meng Yuan, Jie Zou, Lin-Bo Wu, Lang Wei, Taiyu Chen, Nana Zhou, Wenxia Xue, Yafang Zhang, Zongxiang Chen, Keming Hu, Guo-Liang Wang, Wende Liu, Xuebiao Pan, Shimin Zuo
Summary: In this study, RNAi constructs targeting three RBSDV genes (S1, S2, S6) were generated, with RNAi targeting S6 providing almost complete immunity to RBSDV in rice. Additionally, S6RNAi was found to confer strong resistance to SRBSDV as well. The marker-free transgenic lines with S6RNAi in elite rice background show great potential in breeding resistant varieties to both RBSDV and SRBSDV.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Article
Genetics & Heredity
Xiaomei Tang, Xia Wang, Yue Huang, Ling Ma, Xiaolin Jiang, Muhammad Junaid Rao, Yuantao Xu, Ping Yin, Meng Yuan, Xiuxin Deng, Qiang Xu
Summary: Primitive citrus Atalantia buxifolia exhibits high resistance to citrus canker, with natural mutations in AbTFIIA gamma gene and AbLOB1 promoter contributing to this resistance. These mutations could be potential targets for breeding canker-resistant citrus.
Review
Biochemistry & Molecular Biology
Jie Chen, Lihan Wang, Meng Yuan
Summary: MAPK cascades in plants play critical roles in growth, stress responses, and hormone signaling. In rice, the cascades control growth, stress resistance, and signaling functions. Future research on the molecular mechanisms and functions of rice MAPK cascades requires attention to specific concerns.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Letter
Biotechnology & Applied Microbiology
Xudong Li, Renyan Huang, Jiyun Liu, Guoyong Xu, Meng Yuan
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Article
Plant Sciences
Chuanliang Chu, Renyan Huang, Liping Liu, Guilin Tang, Jinghua Xiao, Heejin Yoo, Meng Yuan
Summary: The OsNRAMP1 gene plays an important role in regulating metal ion and reactive oxygen species homeostasis in rice. Removal of this gene leads to enhanced broad-spectrum disease resistance and safety against toxic heavy metals.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Lihan Wang, Jie Chen, Yuqin Zhao, Shiping Wang, Meng Yuan
Summary: This study reveals that OsLIC negatively regulates resistance to certain bacterial pathogens in rice by directly suppressing the transcription of OsWRKY30. It is found that OsMAPK6 interacts with and phosphorylates OsLIC, leading to decreased DNA-binding activity of OsLIC. These results demonstrate that the OsMAPK6-OsLIC-OsWRKY30 module plays an important role in the immune signaling pathway in response to bacterial pathogens in rice.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Hong Li, Yun Zhang, Caiyun Wu, Jinpeng Bi, Yache Chen, Changjin Jiang, Miaomiao Cui, Yuedan Chen, Xin Hou, Meng Yuan, Lizhong Xiong, Yinong Yang, Kabin Xie
Summary: Plants have evolved complex signalling networks to regulate growth and defence responses, but the molecular mechanisms underlying the growth-defence tradeoff are still unclear. This study reveals that OsCPK18 and OsMPK5 can mutually phosphorylate each other and regulate plant height and yield-related traits. Furthermore, they synergistically regulate defence-related genes but differentially regulate development-related genes. Editing the phosphorylation motifs in OsCPK18 and OsCPK4 can improve rice yields and immunity simultaneously.
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Article
Plant Sciences
Shugang Hui, Yinggen Ke, Dan Chen, Lei Wang, Qingqing Li, Meng Yuan
Summary: Rice microRNA156/529-SQUAMOSA PROMOTER BINDING PROTEIN-LIKE7/14/17 modules have pleiotropic effects on many biological pathways, including modulating gibberellin acid signal transduction and enhancing resistance against bacterial pathogens. The miR156/529-regulated OsSPL7/14/17 proteins directly bind to the promoters of target genes and activate their transcription, leading to accumulation of jasmonic acid and salicylic acid signaling pathways, which play crucial roles in plant immunity.
Article
Biotechnology & Applied Microbiology
Miaojing Zhang, Dan Chen, Jingjing Tian, Jianbo Cao, Kabin Xie, Yuqing He, Meng Yuan
Summary: Lipids and lipid metabolites play essential roles in plant-pathogen interactions. The rice lipase OsGELP77 has been found to contribute to both immunity and yield, as it modulates lipid metabolism and hormone signal transduction to regulate pathogen resistance and grain yield in rice.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Agronomy
Pei Li, Lihan Wang, Hongbo Liu, Meng Yuan
Summary: Sugar transportation and sugar-to-starch metabolism are crucial processes in seed development, and OsSWEET genes in rice play essential roles in these processes. Knocking out certain OsSWEET genes resulted in decreased pollen viability and weakened seed traits, indicating their importance in seed development.
Article
Plant Sciences
Wenya Xie, Yinggen Ke, Jianbo Cao, Shiping Wang, Meng Yuan
Summary: The study revealed that OsWRKY53 and OsMYB63 enhance rice resistance to Xoo by strengthening the sclerenchyma cell walls, suggesting that manipulating these candidate genes could be a strategy to improve rice resistance to bacterial pathogens.
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)