Article
Chemistry, Physical
Chunhui Sheng, Xiaodong Guo, Zhuqing Wan, Xiaoqiang Bai, Hao Liu, Xiao Zhang, Ping Zhang, Yunsong Liu, Wenyue Li, Yongsheng Zhou, Longwei Lv
Summary: This study investigated the potential of exosomes derived from human adipose-derived stem cells (hASCs) in promoting osteogenic differentiation for osteoporosis treatment. The study identified the key differentially expressed microRNA between two exosomes and their potential target gene. Results showed that exosomes from osteogenic induction medium had a better effect on promoting osteogenic differentiation than those from proliferation medium. Moreover, miR-335-3p inhibitor-optimized exosomes enhanced osteogenic differentiation in vitro and bone density in vivo, suggesting the potential of engineered exosomes in optimizing exosome-based therapy for osteoporosis.
Article
Cell & Tissue Engineering
Yang Zhou, Xing-Liao Zhang, Shou-Tao Lu, Ning-Yan Zhang, Hai-Jun Zhang, Jing Zhang, Jun Zhang
Summary: This study explores the potential clinical application of hADSCs-Exos encapsulated in PF-127 hydrogel for wound healing. The results showed that PF-127/hADSCs-Exos complexes enhanced skin wound healing, promoted re-epithelialization, increased collagen synthesis and expression of skin barrier proteins, and reduced inflammation. Using PF-127/hADSCs-Exos complexes allows for lower doses and frequency of exosome administration while maintaining therapeutic effects.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biotechnology & Applied Microbiology
Zilong Zhang, Jin Shang, Qinyan Yang, Zonglin Dai, Yuxin Liang, Chunyou Lai, Tianhang Feng, Deyuan Zhong, Haibo Zou, Lelin Sun, Yuhao Su, Su Yan, Jie Chen, Yutong Yao, Ying Shi, Xiaolun Huang
Summary: This study suggests that exosomes derived from human adipose mesenchymal stem cells (hADMSCs-Exo) have significant therapeutic effects on liver fibrosis and cirrhosis. The researchers found that hADMSCs-Exo can inhibit the proliferation of activated hepatic stellate cells, effectively suppress the expression of profibrogenic proteins and epithelial-to-mesenchymal transition (EMT), and improve liver function and reduce liver inflammation. The key mechanism of hADMSCs-Exo in anti-hepatic fibrosis is the inhibition of the PI3K/AKT/mTOR signaling pathway and the regulation of choline metabolism.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Review
Cell & Tissue Engineering
Yao Sun, Guoliang Liu, Kai Zhang, Qian Cao, Tongjun Liu, Jiannan Li
Summary: Exosomes are extracellular vesicles secreted by various cells, mainly composed of lipid bilayers without organelles. Researchers have increasingly focused on using exosomes for drug delivery, especially MSC-EXOs, due to their immune properties, tumor-homing properties, and elastic properties. This review article summarizes the characteristics of MSC-EXOs and introduces production, purification, and drug loading methods to address existing problems and provide suggestions for future studies.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Cell & Tissue Engineering
Yuxiang Zhang, Wei Ju, Hong Zhang, Liu Mengyun, Weiliang Shen, Xiao Chen
Summary: Tendinopathy is a disease caused by the degeneration of tendon tissue, and currently, there is no treatment available to prevent or delay its progression. Recent research suggests that MSCs-derived exosomes (MSCs-exos) may be useful for promoting tendon healing and could be considered as a potential treatment for tendinopathy.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Cell & Tissue Engineering
Shune Xiao, Chunfang Xiao, Yong Miao, Jin Wang, Ruosi Chen, Zhexiang Fan, Zhiqi Hu
Summary: The study demonstrated that hAAM scaffolds incorporated with ADSC-Exos can promote diabetic skin wound healing by regulating inflammation, stimulating vascularization, and promoting the production of extracellular matrix.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell & Tissue Engineering
Jiateng Hu, Yihong Jiang, Xiaoyu Wu, Zhaoyu Wu, Jinbao Qin, Zhen Zhao, Bo Li, Zhijue Xu, Xinwu Lu, Xin Wang, Xiaobing Liu
Summary: ADSC-exos miR-17-5p regulates AAA progression and inflammation via the TXNIP-NLRP3 signaling pathway, providing a novel insight in AAA treatment.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Biochemistry & Molecular Biology
Carl Randall Harrell, Vladislav Volarevic, Valentin Djonov, Ana Volarevic
Summary: AT-MSC-Exos are potential therapeutic agents for neural and retinal diseases by delivering neurotrophins, immunoregulatory factors, and pro-angiogenic miRNAs to alleviate inflammation and promote cell survival.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Gen Li, Yin Zhang, Jiezhou Wu, Renhao Yang, Qi Sun, Yidong Xu, Bo Wang, Ming Cai, Yang Xu, Chengyu Zhuang, Lei Wang
Summary: This study explores the effects and mechanism of adipose stem cells-derived exosomes (ADSCs-Exos) and ADSCs-Exos modified tissue engineering scaffold on bone defect repair. The results demonstrate that ADSCs-Exos promote the proliferation, migration, and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), and ADSCs-Exos modified scaffold enhances the repair of BMSCs and bone defects both in vitro and in vivo.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biophysics
Liyang Wang, Wei Cheng, Jingjing Zhu, Wenyao Li, Danyang Li, Xi Yang, Weixin Zhao, Mingjun Ren, Jieji Ren, Xiumei Mo, Qiang Fu, Kaile Zhang
Summary: Regeneration of urethral defects has been a challenge in clinical practice. A nanoyarn scaffold delivering adipose-derived stem cells' exosomes was fabricated to address this issue. The scaffold showed good biocompatibility and promoted wound healing in experiments.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Chemistry, Medicinal
Hao Pang, Yongting Zhou, Jie Wang, Hao Wu, Xueyi Liu, Feng Gao, Zhibo Xiao
Summary: The study demonstrated that berberine (BBR) has a protective effect on adipose-derived mesenchymal stem cells (ADSCs) after fat grafting by inhibiting apoptosis and autophagy through the AMPK/mTor signaling pathway. Transplantation of human-derived adipose tissue treated with BBR into BALB/c nude mice showed increased survival rate, reduced inflammatory cell infiltration, and fibrosis.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2021)
Article
Biotechnology & Applied Microbiology
Jiawei Zhao, Yue Li, Rongrong Jia, Jinghui Wang, Min Shi, Yugang Wang
Summary: Exosomes, particularly Exo@DEX, have shown promising potential as a drug carrier for autoimmune hepatitis (AIH) by efficiently improving the accumulation of dexamethasone (DEX) in the liver, suggesting potential applications in other diseases as well.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Hsiang-Hao Hsu, Aline Yen Ling Wang, Charles Yuen Yung Loh, Ashwin Alke Pai, Huang-Kai Kao
Summary: This study found that diabetic ASC-Exo enhance cutaneous wound healing by stimulating dermal cell proliferation, keratinocyte proliferation, and angiogenesis. The diabetic ASC-Exo also stimulate resident monocytes/macrophages to secrete more TGF-beta 1 and activate the TGF-beta/Smad3 signaling pathway.
Article
Cell & Tissue Engineering
Li Han, Zhirong Zhao, Xingyun Chen, Ke Yang, Zhen Tan, Zhu Huang, Lichen Zhou, Ruiwu Dai
Summary: In this study, it was found that human umbilical cord mesenchymal stem cells-exosomes (hucMSC-Exs) can colonize injured pancreatic tissue and facilitate the repair of pancreatic tissue by inhibiting cell apoptosis and controlling inflammatory response.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Pharmacology & Pharmacy
Jafar Rezaie, Vahid Nejati, Monireh Mahmoodi, Mahdi Ahmadi
Summary: Extracellular vesicles (EVs), particularly MSCs-EVs, have shown great potential as a drug delivery system in nanomedicine. They can selectively deliver therapeutic agents to target cells and improve various diseases. However, there are still challenges that need to be addressed for the clinical translation of modified MSCs-EVs.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)