期刊
BIOCHEMICAL JOURNAL
卷 467, 期 -, 页码 387-398出版社
PORTLAND PRESS LTD
DOI: 10.1042/BJ20141498
关键词
cap/poly(A); cell free protein synthesis systems (CFPS); in vitro translation; internal ribosome entry site (IRES); ribosomes; reticulocyte lysate
资金
- Agence Nationale de Recherche sur le Sida [AO2014-14409]
- region Rhone Alpes
Cell free protein synthesis systems (CFPS) have been widely used to express proteins and to explore the pathways of gene expression. In the present manuscript, we describe the design of a novel adaptable hybrid in vitro translation system which is assembled with ribosomes isolated from many different origins. We first show that this hybrid system exhibits all important features such as efficiency, sensitivity, reproducibility and the ability to translate specialized mRNAs in less than 1 h. In addition, the unique design of this cell free assay makes it highly adaptable to utilize ribosomes isolated from many different organs, tissues or cell types.
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