Article
Reproductive Biology
Dulama Richani, Robert B. Gilchrist
Summary: Oocytes are maintained in a state of meiotic arrest until ovulation is triggered. Manipulation of cyclic nucleotides such as cGMP and cAMP can influence meiotic arrest and improve oocyte developmental competence. Pre-IVM modulation of cGMP and/or cAMP has shown consistent improvement in oocyte developmental competence.
BIOLOGY OF REPRODUCTION
(2022)
Review
Obstetrics & Gynecology
Robert B. Gilchrist, Tuong M. Ho, Michel De Vos, Flor Sanchez, Sergio Romero, William L. Ledger, Ellen Anckaert, Lan N. Vuong, Johan Smitz
Summary: Recent advances in understanding oocyte biology and ovulation from animal studies have led to the development of biphasic IVM approaches, which involve pre-culturing immature oocytes before routine in vitro maturation. This novel procedure has the potential to significantly impact human ART practices. This review examines the scientific advances in ovarian biology, biphasic IVM procedures, and reports on outcomes from animal and clinical human data.
HUMAN REPRODUCTION UPDATE
(2023)
Article
Biochemistry & Molecular Biology
Mateusz Kwiatkowski, Aloysius Wong, Anna Kozakiewicz, Christoph Gehring, Krzysztof Jaworski
Summary: This study uncovered the elusive cyclic nucleotide phosphodiesterases (PDEs) in plants and identified a potential PDE, Arabidopsis thaliana K+-Uptake Permease (AtKUP5). The PDE activity was confirmed through molecular docking analysis and was found to be stimulated by the Ca2+/CaM complex.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Endocrinology & Metabolism
Lixia Zhu, Qiyu Yang, Huizi Jin, Juepu Zhou, Meng Wang, Liu Yang, Zhou Li, Kun Qian, Lei Jin
Summary: This study identified different phenotypes in patients with oocyte maturation arrest (OMA), and found that clinical outcomes were associated with the presence of gene mutations and the classification of oocyte phenotypes. A triage system was proposed to optimize resource allocation and maximize patient benefit.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Endocrinology & Metabolism
Dongmei Deng, Juan Xie, Yin Tian, Ling Zhu, Xuemei Liu, Junxia Liu, Guoning Huang, Jingyu Li
Summary: Acquisition of germinal vesicle (GV) stage oocytes for fertility preservation (FP) offers several benefits over in vivo matured oocyte cryopreservation following ovarian stimulation. Two approaches for GV oocytes are available: vitrification before in vitro maturation (IVM) at the GV stage or post-IVM at the metaphase II (MII) stage. This study found that oocyte vitrification at the MII stage after IVM is more suitable for patients who require immediate FP.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Review
Cell Biology
Xiaoyi Wang, Melissa E. Pepling
Summary: This article discusses the meiotic process of female mammalian oocytes during embryonic development, highlighting the events and mechanisms from meiotic prophase one to metaphase two. It emphasizes the importance of understanding the mechanisms regulating meiotic progression to improve treatments for female infertility, as errors in meiosis can lead to aneuploidy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Saffet Ozturk
Summary: This review comprehensively evaluates the molecular determinants of developmental and permanent arrests occurring in mammalian oocytes, and discusses potential approaches for inducing permanent arrests to obtain competent oocytes.
Article
Cell Biology
Daniela R. Chavez, Pei-Chih Lee, Pierre Comizzoli
Summary: The study identified BRD2 and ASRGL1 as critical factors for oocyte meiotic competence, while NPM1 did not show significant involvement in the process. Inhibition of BRD2 and NPM1 resulted in oocytes being unable to mature to the metaphase II stage, indicating their importance in oocyte development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Endocrinology & Metabolism
Zhenle Pei, Ke Deng, Congjian Xu, Shuo Zhang
Summary: This article summarizes the cellular and molecular mechanisms involved in oocyte development and maturation, including meiotic arrest and resumption, as well as cytoplasmic events associated with oocyte quality. Understanding these mechanisms is crucial for understanding the female reproductive system and for future research and potential treatment approaches.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ralf Poehland, Jens Vanselow, Fabiana Melo Sterza
Summary: This study investigated the presence of HES1 in both compartments of bovine cumulus oocyte complexes (COCs) and its distribution after in vitro maturation (IVM). HES1 mRNA was detectable in oocytes but not in cumulus cells, while the protein was mainly visible in cumulus cells. GFP-HES1-mRNA injection showed signal in both oocytes and cumulus cells, suggesting protein transfer from oocytes to cumulus cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Kaori Nozawa, Zian Liao, Yuhkoh Satouh, Ting Geng, Masahito Ikawa, Diana Monsivais, Martin M. Matzuk
Summary: Wee1-like protein kinase 2 (WEE2) is an oocyte-specific protein tyrosine kinase involved in the regulation of oocyte meiotic arrest. Knockout mouse models were developed to test the impact of Wee2 loss-of-function on female fertility. While a frameshift mutation had no effect, a larger deletion resulted in reduced litter size in homozygous Wee2 female knockout mice. Overall, loss of Wee2 had only minor effects on fertility in mice, suggesting alternative approaches should be considered for contraceptive development.
Review
Obstetrics & Gynecology
Dulama Richani, Kylie R. Dunning, Jeremy G. Thompson, Robert B. Gilchrist
Summary: Metabolism is crucial for oocyte quality, with glucose being essential for COC maturation and energy production. Mitochondria are the main ATP source in oocytes, while cumulus cells play a vital role in maintaining ATP levels. New insights in oocyte lipid metabolism and innovative strategies in IVM have shown positive effects on oocyte competence.
HUMAN REPRODUCTION UPDATE
(2021)
Article
Agriculture, Dairy & Animal Science
Hao Cheng, Xue Sun, Fei Chen, Liu-Zhu Pan, Guo-Liang Wang, Hong-Jie Yuan, Zhong-Le Chang, Jing-He Tan
Summary: The study showed that treatment with roscovitine, SN chromatin configuration, and female sexual maturity can significantly improve the developmental potential of mouse oocytes by regulating the expression of competence-related genes, indicating Oct4, Stella, Mater, Zarl, Mapk8, and Bcl2 as beneficial genes and Foxj2, Ship], and Bax as detrimental genes.
JOURNAL OF REPRODUCTION AND DEVELOPMENT
(2021)
Article
Biology
Daniela Nogueira, Carole Fajau-Prevot, Muriel Clouet, Patrick Assouline, Marion Deslandres, Marie Montagut
Summary: To ensure patient care in an oncological fertility preservation programme, the best technology must be provided to suit the patients' clinical conditions. In vitro oocyte maturation (IVM) and ovarian tissue cryopreservation (OTC) are effective methods for fertility preservation in women requiring urgent oncological treatment. Further research is needed to fully assess the technical consistency, efficacy, and safety of IVM using immature oocytes collected through different methods.
Article
Nutrition & Dietetics
Kota Shiozawa, Misato Maeda, Hsin-Jung Ho, Tomoko Katsurai, Md Zakir Hossain Howlader, Kimiko Horiuchi, Yumi Sugita, Yusuke Ohsaki, Afifah Zahra Agista, Tomoko Goto, Michio Komai, Hitoshi Shirakawa
Summary: The study found that supplementation of biotin increased testosterone levels in mice and testis-derived cells. This effect was mediated by the activation of adenylate cyclase and protein kinase A. These findings suggest that biotin may have the potential to improve age-related male syndromes associated with declining testosterone production.
Article
Cell Biology
Joanna M. G. Souza-Fabjan, Ribrio I. T. P. Batista, Luciana M. Melo, Marcos A. L. Oliveira, Maiana S. Chaves, Jeferson F. Fonseca, Vicente J. F. Freitas
Summary: The study compared laparotomy and nonsurgical embryo recovery techniques in Caninde goats for creating an embryo bank. The results showed that the nonsurgical method was similar to the laparotomy technique with fewer health risks and sequels for the females. This study may help in vivo embryo production programs in the Caninde breed and ensure genetic diversity preservation.
BIOPRESERVATION AND BIOBANKING
(2022)
Article
Cell Biology
Jeferson F. Fonseca, Gabriel B. Vergani, Monalisa S. D. Lima, Kleibe M. Silva, Alexandre W. U. Monteiro, Alexandre F. Ramos, Bruna R. C. Alves, Joanna M. G. Souza-Fabjan, Maria E. F. Oliveira, Ribrio I. T. P. Batista
Summary: This study evaluated the feasibility and efficiency of using superovulation combined with a less-invasive embryo collection technique in locally adapted Brazilian breeds of sheep and goats. The results showed that this method was effective in producing cryopreservable embryos and maintaining donor fertility.
BIOPRESERVATION AND BIOBANKING
(2022)
Article
Agriculture, Dairy & Animal Science
Marcela S. Rocha, Jeferson F. Fonseca, Claudio A. Oliveira, Ana Lucia R. S. Maia, Paulo Sergio C. Rangel, Maria Emilia F. Oliveira, Joanna M. G. Souza-Fabjan
Summary: The study found that premature regression of corpus luteum (PRCL) was not associated with donor age, body condition score, number of MOET repetitions, or season, but it affected around 25% of ewes undergoing MOET procedures. Future alternatives to overcome this obstacle need to be developed.
Article
Veterinary Sciences
Juliana Dantas Rodrigues Santos, Ribrio Ivan Tavares Pereira Batista, Rodolfo Ungerfeld, Augusto Ryonosuke Taira, Caroline Gomes do Espirito Santo, Joanna Maria Goncalves Souza-Fabjan, Daniel Andrews de Moura Fernandes, Mario Felipe Alvarez Balaro, Isabel Oliveira Cosentino, Viviane Lopes Brair, Pedro Henrique Nicolau Pinto, Ana Beatriz da Silva Carvalho, Jeferson Ferreira da Fonseca, Felipe Zandonadi Brandao
Summary: The study found that using hormonal protocols for cervical dilation in sheep reduces the efficiency of embryo collection, induces luteolysis, and causes a transient alteration in embryo gene expression, but has minimal impact on embryo morphological quality, development stage, or incidence of apoptosis.
Article
Agriculture, Dairy & Animal Science
Augusto Ryonosuke Taira, Ribrio Ivan Tavares Pereira Batista, Juliana Dantas Rodrigues Santos, Pedro Henrique Nicolau Pinto, Mario Felipe Alvarez Balaro, Caroline Gomes do Espirito Santo, Viviane Lopes Brair, Joanna Maria Goncalves Souza-Fabjan, Rodolfo Ungerfeld, Jeferson Ferreira da Fonseca, Felipe Zandonadi Branda
Summary: This study aimed to compare the effects of medroxyprogesterone acetate (MPA) and progesterone (P4) in superovulation treatments. The results showed that supplementation with P4 during the first follicular wave of the estrous cycle improves embryo viability and alters the expression of the TGFB1 gene.
ANIMAL REPRODUCTION SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Maria Amelia Ferrao Pupin, Maria Emilia Franco Oliveira, Gabriel Brun Vergani, Monalisa Sousa Dias Lima, Kleibe de Moraes Silva, Alexandre Weick Uchoa Monteiro, Alexandre Floriani Ramos, Ribrio Ivan Tavares Pereira Batista, Joanna Maria Gonsalves Souza-Fabjan, Pawel Mieczyslaw Bartlewski, Jeferson Ferreira Fonseca
Summary: This study examined the differences in ovarian responses and in vivo embryo production among three naturalized Brazilian sheep breeds. The study found that there were differences in ovarian follicle numbers and blood flow indices among the breeds, but no significant differences were observed in embryo yields and quality following superovulation.
SMALL RUMINANT RESEARCH
(2022)
Article
Reproductive Biology
Augusto Ryonosuke Taira, Felipe Zandonadi Brandao, Viviane Lopes Brair, Isabel Oliveira Cosentino, Felipe Seabra Cardoso Leal, Ana Clara Sarzedas Ribeiro, Mario Felipe Alvarez Balaro, Ribrio Ivan Tavares Pereira Batista, Joanna Maria Goncalves Souza-Fabjan, Jeferson Ferreira da Fonseca, Rodolfo Ungerfeld
Summary: The aim of this study was to compare preovulatory follicular development and superovulatory outcomes in superovulated ewes with and without stimulation by rams. The results showed that ewes stimulated by rams had a higher number of large follicles, larger follicle diameter, and greater embryo viability rate compared to ewes without ram stimulation. The number of corpora lutea, follicular cysts, recovered structures, viable embryos, and degenerated and unfertilized structures were similar between the two groups.
Review
Reproductive Biology
Gabriela Ramos Leal, Clara Ana Santos Monteiro, Luciano de Rezende Carvalheira, Joanna M. G. Souza-Fabjan
Summary: Simulated Physiological Oocyte Maturation (SPOM) is an in vitro method that mimics the physiological events of oocyte maturation using cAMP modulators. This study systematically analyzes the literature on the use of SPOM and compares the outcomes to the original study. The results indicate difficulties in reproducing the SPOM system worldwide and suggest that meiosis arrest alone is not sufficient for successful SPOM application. The different supplements used in the IVM medium and their interaction with modulators may impact experimental success.
Article
Reproductive Biology
Leticia Pereira Alcaraz, Lucia Prellwitz, Gutemberg Alves, Joanna Maria Gonsalves Souza-Fabjan, Angelo Jose Burla Dias
Summary: Modulation of the PI3K/AKT/PTEN pathway has the potential to improve overall efficiency of in vitro embryo production, but its effects on meiosis resumption are still uncertain.
Article
Agriculture, Dairy & Animal Science
J. H. Dias, J. D. Goncalves, A. M. Arrais, J. M. G. Souza-Fabjan, R. Bastos, R. I. T. P. Batista, L. G. B. Siqueira, M. E. F. Oliveira, J. F. Fonseca
Summary: This study investigated the effectiveness of different doses of estradiol benzoate (EB) in promoting cervical relaxation and their effects on luteal function and outcomes of non-surgical embryo recovery (NSER) in sheep. The results showed that EB treatments did not affect NSER success but they did affect luteal function by altering progesterone and estradiol concentrations.
DOMESTIC ANIMAL ENDOCRINOLOGY
(2023)
Article
Reproductive Biology
Jasmine B. S. Pinheiro, Lucas M. Figueira, Lucas F. L. Correia, Thais A. Oliveira, Viviane L. Brair, Felipe F. P. C. Barros, Fabio O. Ascoli, Ribrio I. T. P. Batista, Felipe Z. Brandao, Joanna M. G. Souza-Fabjan
Summary: This study assessed the effect of different coasting times on the quantity and quality of cumulus-oocyte complexes in sheep. The results showed that 60 hours of coasting time had a positive impact on the quality of cumulus-oocyte complexes in sheep.
Article
Agriculture, Dairy & Animal Science
Ana Clara Sarzedas Ribeiro, Augusto Ryonosuke Taira, Vanessa Costa Santos, Viviane Lopes Brair, Maria Paula Fassarella Lopes, Mario Felipe Alvarez Balaro, Joanna Maria Goncalves Souza-Fabjan, Jeferson Ferreira da Fonseca, Rodolfo Ungerfeld, Felipe Zandonadi Brandao
Summary: The aim of this study was to determine the effectiveness of meloxicam with or without dipyrone on the welfare of ewes subjected to non-surgical embryo recovery (NSER). The results showed that NSER induced stress and pain in ewes, affecting their welfare. The administration of meloxicam was found to be ineffective in reducing stress and pain, while the association of dipyrone had only slight effects.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Agriculture, Dairy & Animal Science
J. H. Dias, G. B. Vergani, J. D. Goncalves, T. A. Oliveira, J. M. Penitente-Filho, V. S. A. Pereira, S. N. Esteves, A. R. Garcia, R. I. T. P. Batista, M. E. F. Oliveira, J. M. G. Souza-Fabjan, J. F. Foseca
Summary: This study evaluated the effect of different doses of pFSH on follicular growth, superovulatory response, and embryo yield in White Dorper sheep. The results showed that all treatment groups had higher numbers of large follicles on D8 and D9. Non-surgical embryo recovery was successful in a high percentage of females, and the number of transferable embryos did not differ among groups.
SMALL RUMINANT RESEARCH
(2023)
Article
Cell Biology
L. P. Alcaraz, P. V. S. Pereira, T. A. Oliveira, L. F. L. Correia, E. M. Vasconcelos, F. Z. Brandao, J. M. G. Souza-Fabjan
Summary: This study aimed to assess the effect of adding AFP I to cryopreservation medium on domestic cat semen. The results showed that adding 0.1 and 0.5 μg/ml of AFP I did not have consistent beneficial effects on the overall cryotolerance of domestic cat sperm.
Article
Agriculture, Dairy & Animal Science
Maria Emilia Franco Oliveira, Tarcilla Dutra Barbosa Caldas, Juliana Nascimento Duarte Rodrigues, Gabriel Brun Vergani, Paulo Sergio Cerqueira Rangel, Luciana Vieira Esteves, Joanna Maria Gonsalves Souza-Fabjan, Felipe Zandonadi Brandao, Jeferson Ferreira Fonseca
Summary: This study examined the effects of reinsertion of a progesterone (P4) device during the early luteal phase on luteal function and embryo yield in superovulated crossbred ewes. The results showed that reinsertion of the P4 device during the early luteal phase increased progesterone concentrations and resulted in a higher yield of embryos.
ANIMAL REPRODUCTION SCIENCE
(2023)