Article
Chemistry, Analytical
Miaosen Zhang, Shan He, Wei Pang, Wei Wei, Feng Zhou, Xiaoyu Wu, Hang Qi, Xuexin Duan, Yanyan Wang
Summary: This research demonstrates a fast purification and delivery method of quantum dots into living cells using acoustic streaming and a gigahertz resonator. The results show that the impurities in the carbon dots can be efficiently extracted and delivered into cells, and the acoustic streaming can accelerate the bioreaction inside cells, enhancing the biosensing of metal ions in single living cells.
Article
Chemistry, Multidisciplinary
Ahmed Z. Sihorwala, Alexander J. Lin, Jeanne C. Stachowiak, Brian Belardi
Summary: Living cells release molecular signals for communication and coordination, which can be mimicked by nonliving synthetic cells for material exchange and drug delivery. This study focuses on connexon nanopores as a platform for rapid release of molecules from synthetic cells, using light-sensitive liposomes and proteases to trigger the assembly and activity of the nanopores.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Nanoscience & Nanotechnology
Hang Wu, Zifan Tang, Rui You, Shuting Pan, Wenpeng Liu, Hongxiang Zhang, Tiechuan Li, Yang Yang, Chongling Sun, Wei Pang, Xuexin Duan
Summary: This study investigates the behavior of micro/nanoscale particles trapped in gigahertz acoustic streaming tweezers (AST) through theoretical analyses, 3D simulations, and microparticle tracking experiments. The particle motion in the vortices is found to be mainly influenced by the balance between the acoustic streaming drag force and the acoustic radiation force. This work provides fundamental design principles for AST-based lab-on-a-chip systems for various applications.
NANOTECHNOLOGY AND PRECISION ENGINEERING
(2022)
Article
Chemistry, Analytical
Junjun Lei, Gaokun Zheng, Zhen Yao, Zhigang Huang
Summary: In this study, the authors analyze the outer acoustic streaming flows generated over a fluid-solid interface using theoretical and numerical methods. They explore the differences between symmetric and asymmetric acoustic resonances and find that in symmetric resonances, the limiting velocities always drive fluids from the acoustic pressure node towards adjacent antinodes. However, in asymmetric resonances, the outer acoustic streaming is spatially separated from the acoustic radiation force and is independent of the shift in acoustic pressure. These findings have implications for acoustics streaming flow control and particle manipulation in microfluidics applications.
Article
Chemistry, Multidisciplinary
Remy Cochereau, Viviana Maffeis, Elena C. dos Santos, Emanuel Lortscher, Cornelia G. Palivan
Summary: The production, stability, and membrane diffusion properties of polymer GUVs are greatly improved by the introduction of DNA-origami nanopores (DoNs), leading to high control over these essential properties. The DoN-GUVs produced by microfluidics exhibit narrow size distribution, high encapsulation efficiency, and long-term stability, enabling the tuning of molecular transport rates and the investigation of cellular processes and signaling.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Polymer Science
Bingbing Zhao, Yuting Yan, Junmei Zhang, Enping Chen, Ke Wang, Changshun Zhao, Yinan Zhong, Dechun Huang, Zhiqin Cui, Dawei Deng, Congying Gu, Wei Chen
Summary: A novel zwitterionic triblock copolymer was designed and synthesized, showing well-defined structure and controlled molecular weight; the copolymer could self-assemble into polymersomes with good biocompatibility and cell transmembrane ability; cytochrome c-loaded polymersomes exhibited potent cancer inhibitory activity and apoptosis-inducing ability against HeLa cells.
Review
Chemistry, Multidisciplinary
Arnab Banerjee, Miguel Lino, Carlos Jesus, Quelia Ribeiro, Antero Abrunhosa, Lino Ferreira
Summary: Extracellular vesicles (EVs) act as communication vehicles for exchanging bioactive molecules between cells. Understanding their role in cellular communication is crucial for evaluating their biological, diagnostic, and therapeutic potential. Controlled release of EVs for regenerative medicine applications has been explored, and evaluating their release profile is important for correlating with biological activity. This article provides an overview of EV imaging platforms, discusses different EV labeling processes, and highlights the relevance of imaging platforms in studying the tropism, biological role, and release profile of EVs.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Polymer Science
Christos Kostaras, Dimitra Patroni, Nikolaos Spiliopoulos, Dimitrios L. Anastassopoulos, Alexandros Vradis, Chris Toprakcioglu
Summary: We have investigated the flow characteristics of liquids through anodic alumina membranes with nanopores covered with copolymer brushes. The flow behavior can be accurately described by Poiseuille's law, and the hydrodynamic brush height within the nanopores can be determined. The height of the brushes varies with the molecular weight of the copolymer in a power law relationship. By adjusting the solvent quality, the flow rate can be significantly changed, allowing for flow control through the response of the brush height to the quality of the solvent.
Article
Acoustics
E. R. Cardozo de Oliveira, C. Xiang, M. Esmann, N. Lopez Abdala, M. C. Fuertes, A. Bruchhausen, H. Pastoriza, B. Perrin, G. J. A. A. Soler-Illia, N. D. Lanzillotti-Kimura
Summary: We report mesoporous titanium dioxide-based acoustic resonators with resonances up to 90 GHz and quality factors from 3 to 7. Numerical simulations show good agreement with picosecond ultrasonics experiments. We also study the effect of changes in the speed of sound on the resonator's performance, which can be induced by liquid infiltration into the mesopores. These findings constitute the first step towards engineering reconfigurable optoacoustic sensors based on mesoporous thin films.
Article
Chemistry, Multidisciplinary
Alena Ivanova, Lukas Badertscher, Gwen O'Driscoll, Joakim Bergman, Euan Gordon, Anders Gunnarsson, Camilla Johansson, Michael J. Munson, Cristiana Spinelli, Sara Torstensson, Liisa Vilen, Andrei Voirel, John Wiseman, Janusz Rak, Niek Dekker, Elisa Lazaro-Ibanez
Summary: Efficient and targeted delivery of therapeutic agents is achieved by genetically modifying extracellular vesicles (EVs) to improve cargo loading and specific cell targeting. This study demonstrates successful targeting of EVs to human primary hepatocytes using engineered EVs displaying targeting ligands. Furthermore, the authors enhance EV targeting to cells overexpressing a specific receptor by decorating EVs with an antibody recognizing the receptor. The study also shows improved loading and functional delivery of Cre recombinase into recipient cells using different protein-engineering approaches.
Article
Chemistry, Multidisciplinary
Shan He, Wei Pang, Xiaoyu Wu, Yang Yang, Wenjun Li, Hang Qi, Chongling Sun, Xuexin Duan, Yanyan Wang
Summary: This study proposes a hydrodynamic intracellular delivery system based on the gigahertz acoustic streaming effect, which allows for tunable delivery range by adjusting the acoustic streaming radius. With the assistance of acoustic streaming, AuNRs with different modifications can be targetedly delivered to specific organelles. Additionally, the large quantities of AuNRs introduced via acoustic streaming demonstrate a photothermal effect, which has the potential to shorten the treatment cycle of photothermal therapy. This efficient hydrodynamic technology provides a new idea for precise medical treatment.
Article
Biochemical Research Methods
Shan He, Zefang Wang, Wei Pang, Chang Liu, Miaosen Zhang, Yang Yang, Xuexin Duan, Yanyan Wang
Summary: The development of a novel non-invasive neurite outgrowth modulation method based on a controllable acoustic streaming effect induced by an electromechanical gigahertz resonator microchip is reported. The method demonstrates the potential for precise single-cell modulation and rapid disease treatment strategies through promoting neurite outgrowth and facilitating the entry of nerve growth factor (NGF) into cells.
Article
Acoustics
Kuai Yu, Yiqi Jiang, Yungao Chen, Xiaoyan Hu, Junlei Chang, Gregory V. Hartland, Guo Ping Wang
Summary: Membrane viscosity is an important property of cell biology, and we investigated it at gigahertz frequencies through damping of acoustic vibrations of gold nanoplates. The experiments showed that cell membranes exhibit viscoelasticity with an estimated relaxation time. Furthermore, we demonstrated the potential of using membrane viscoelasticity at gigahertz frequencies to differentiate cancerous cells from normal cells, with cancer cells having lower viscosity compared to healthy cells. These findings have promising applications in cell diagnosis.
Article
Chemistry, Multidisciplinary
Vadim Krivitsky, Adva Krivitsky, Valeria Mantella, Maya Ben-Yehuda Greenwald, Devanarayanan Siva Sankar, Jil Betschmann, Johannes Bader, Nicole Zoratto, Kento Schreier, Sarah Feiss, Dario Walker, Jorn Dengjel, Sabine Werner, Jean-Christophe Leroux
Summary: This study presents an electrochemical all-in-one device that can rapidly capture, load, and release extracellular vesicles (EVs). The device, composed of antibody-coated microstructured electrodes, effectively isolates EVs from various biofluids and improves EV loading with polyplexes. This portable device offers a lab-on-a-chip approach for efficient EV isolation and manipulation.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Lea Chazot-Franguiadakis, Joelle Eid, Marius Socol, Bastien Molcrette, Philippe Guegan, Marylene Mougel, Anna Salvetti, Fabien Montel
Summary: This study demonstrates the capability of combining hydrodynamical driving and optical sensing to identify and quantify viral particles in a biological sample. The method is simple and rapid, requiring only fluorescent labeling of the particles, and can be applied to a wide range of virus types. The system operates in real time and provides low concentration error and limit of detection at the single particle level.
Article
Physics, Condensed Matter
Mark V. de Ruiter, Nico J. Overeem, Gaurav Singhai, Jeroen J. L. M. Cornelissen
JOURNAL OF PHYSICS-CONDENSED MATTER
(2018)
Article
Chemistry, Multidisciplinary
Nico J. Overeem, Pieter H. (Erik) Hamming, Jurriaan Huskens
Review
Chemistry, Multidisciplinary
Nico J. Overeem, Erhard van der Vries, Jurriaan Huskens
Summary: This review explores how influenza viruses navigate mucus and recognize host cells through multivalent interactions. The surface proteins of the influenza A virus facilitate specific binding to host cells, and the importance of these multivalent interactions in crossing mucus and entering host cells is discussed. Techniques to measure the avidity and receptor specificity of influenza viruses are also reviewed.
Article
Chemistry, Multidisciplinary
Nico J. Overeem, P. H. (Erik) Hamming, Malte Tieke, Erhard van der Vries, Jurriaan Huskens
Summary: The method of multivalent affinity profiling (MAP) allows for quantitatively assessing the binding of influenza A virus (IAV) to receptor density gradients, determining the threshold receptor density, and visualizing and assessing the superselective binding of IAVs. The threshold receptor density increases with increasing flow rate, indicating that the superselective binding of IAV is influenced by shear force and experimental conditions. This method enables not only comparative studies of virus-receptor interactions but also fundamental studies on the mechanism of superselectivity.
Article
Nanoscience & Nanotechnology
P. H. Erik Hamming, Nico J. Overeem, Kevin Diestelhorst, Tren Fiers, Malte Tieke, Gael M. Vos, Geert-Jan P. H. Boons, Erhard van der Vries, Stephan Block, Jurriaan Huskens
Summary: Influenza viruses use surface motility to find or create locations for cell entry and enhance cellular uptake. The motility is expected to depend on the receptor density, and surface gradients with varying receptor densities can mimic the local variations on the cell surface. By tracking individual virus particles over surfaces with receptor density gradients, researchers found that surface mobility and the probability of sticking are significantly dependent on receptor density. The findings provide insights into how the virus locates high-receptor density patches for cell entry. Overall, the study scores 8 out of 10.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Nico J. Overeem, P. H. Erik Hamming, Oliver C. Grant, Daniele Di Iorio, Malte Tieke, M. Candelaria Bertolino, Zeshi Li, Gael Vos, Robert P. de Vries, Robert J. Woods, Nicholas B. Tito, Geert-Jan P. H. Boons, Erhard van der Vries, Jurriaan Huskens
ACS CENTRAL SCIENCE
(2020)
Review
Chemistry, Multidisciplinary
P. H. (Erik) Hamming, Nico J. Overeem, Jurriaan Huskens