Article
Biochemistry & Molecular Biology
Anton M. Solovjev, Ivan I. Galkin, Olga Yu Pletjushkina, Alexey Medvedko, Shulin Zhao, Ivan Yu Sakharov
Summary: A sensitive and specific heterogeneous assay for quantitation of miRNA-39 was developed using ICSDPR, polyperoxidase conjugated with streptavidin, and enhanced chemiluminescence. The assay demonstrated a low detection limit, low coefficient of variation, high specificity, and negligible matrix effect, making it useful for estimating miRNA purification yield from cells.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Analytical
Linyan Li, Xiaojun Fang, Jingqing Le, Yanhui Zheng, Xiarong Tan, Zhou Jiang, Hongxia Li, Jianguo Xu, Huo Xu
Summary: In this study, a cascade catalytic hairpin assembly (CCHA) method was developed for sensitive and selective detection of miRNA. The method showed high assay sensitivity, excellent sequence specificity, and the ability for intracellular imaging, demonstrating promising potential for biosensing, clinical diagnosis, or prognosis.
Article
Chemistry, Analytical
Zhang Zhang, Li Zhang, Youqiang Wang, Juan Yao, Ting Wang, Zhi Weng, Liu Yang, Guoming Xie
Summary: This study introduces an ultrasensitive electrochemical miRNA biosensor based on strand displacement reaction circuits and strand displacement polymerization reaction, demonstrating high sensitivity and discriminatory capability towards let 7a for early cancer screening, providing a new research avenue.
ANALYTICA CHIMICA ACTA
(2021)
Article
Food Science & Technology
Jing Li, Yong-Wei Feng, Li-Jun Huang, Rui Jiang, Xiao-Fang Shen
Summary: Proper labeling of meat products is crucial for consumers who want to avoid specific meat species for cultural or religious reasons. The newly developed SDPICA detection method can identify undeclared pork components in meat products within 20 minutes at 37 degrees Celsius, with a sensitivity as low as 0.1% in multi-component meat mixtures.
Review
Chemistry, Inorganic & Nuclear
Fan Yang, Huiting Lu, Xiangdan Meng, Liping Li, Wenhao Dai, Lingzhi Yang, Ruiping Zhang, Haifeng Dong, Xueji Zhang
Summary: The spatial-temporal and dynamic expression profiles of intracellular microRNA are crucial for understanding cellular biological behaviors and monitoring pathological processes. Nucleic acid probe systems have been developed for various biological tasks in living cells due to their intrinsic biosecurity, biocompatibility, ease of synthesis, and ability for efficient recognition and signal amplification. This review highlights recent achievements in nucleic acid probe systems for intracellular miRNA sensing and discusses approaches for improving detection sensitivity, selectivity, and accuracy.
COORDINATION CHEMISTRY REVIEWS
(2023)
Article
Biophysics
Lok Ting Chu, Hoi Kwan Kwong, Hogi Hartanto, Ting-Hsuan Chen
Summary: This study reported a method for detecting intracellular sodium ions by activating DNAzyme through endogenous mRNA, successfully achieving the detection of sodium ions in different phenotypic cells and observing an increase in their levels by increasing the permeability of the cell membrane.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Saiwei Wu, Abdu Ahmed Abdullah Al-Maskri, Qun Li, Jiatong Liu, Sheng Cai
Summary: A novel ligation-based loop-mediated isothermal amplification (LAMP) method was developed for miRNA detection. It utilized stem-loop structure DNA linker A/B probes to establish a rapid and ultrasensitive miRNA-LAMP system. This method can detect miRNA in a linear range of seven orders of magnitude, with a detection limit as low as 100 fM. It provides a new path for highly sensitive quantitative analysis of miRNA, which can bring convenience to clinical diagnosis and prognostic research.
INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Jiemei Zhao, Xiaoqin Zhao
Summary: This paper describes a simple and sensitive miRNA detection method based on dual signal recycles, which can be applied for early clinical diagnosis and biological research.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Hua Chai, Peng Miao
Summary: A novel electrochemical strategy combining strand displacement amplification and DNA nanostructures was developed for ultrasensitive detection of ctDNA, achieving a limit of detection as low as 48 amol/L. This proposed electrochemical biosensor shows great potential for clinical applications.
CHINESE CHEMICAL LETTERS
(2021)
Article
Chemistry, Analytical
Zhen Chi, Yinhuan Wu, Lihong Chen, Hao Yang, Mohammad Rizwan Khan, Rosa Busquets, Ning Huang, Xin Lin, Ruijie Deng, Weizhu Yang, Jingyao Huang
Summary: This study developed a detection method for early diagnosis using circulating miRNA, which achieved continuous amplification of strand displacement amplification through a CRISPR-Cas14a system, enabling efficient detection of miR-21 in a short period of time and discrimination between cholangiocarcinoma patients and healthy individuals.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Yifei Chen, Lizhen Ye, Hui Chen, Tingting Fan, Cheng Qiu, Yan Chen, Yuyang Jiang
Summary: miRNA is a promising biomarker for cancer diagnosis, and the development of new detection methods for it has been a hot topic recently. In this study, we identified miR-625-5p as a potential biomarker for colorectal cancer through expression analysis and phenotypic experiments. We also developed a novel detection method based on strand displacement amplification (SDA) to monitor miR-625-5p levels, which showed good response and high specificity.
Article
Chemistry, Analytical
Zongwei Zhu, Hongqian Zhang, Xiaoxue Dong, Meng Lin, Chuanxu Yang
Summary: This study proposes a novel biosensing system for fluorescence imaging of miR21 in cancer cells. The system consists of an optimized dsDNA probe for miR21 sensing and a carrier vesicle for efficient intracellular delivery. The system showed high accuracy and sensitivity for miR21 recognition and could discriminate cancer cells from normal cells. Therefore, it holds great promise for early cancer diagnosis.
Article
Chemistry, Multidisciplinary
Yajun Zhang, Shen Yan, Zhaoxin Chen, Xin Jiang, Shuang Rao, Zhuoran Jiang, Shanshan Qin, Xiang Zhou, Yuhao Du
Summary: This study proposes a sensitive method for visually detecting telomerase activity using a gold nanoparticle probe. The probe measures the fluorescence signals to determine the presence of telomerase.
CHINESE JOURNAL OF CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Xiao-Long Zhang, Sha-Sha Li, Wei-Wei Liu, Ling-Qi Kong, Ya-Qin Chai, Ruo Yuan
Summary: A high-efficiency mismatch-fueled catalytic multiple-arm DNA junction assembly (M-CMDJA) is developed for rapid detection and ultrasensitive intracellular imaging of miRNA. It possesses larger kinetic and thermodynamic favorability, resulting in higher efficiency and rates compared to traditional nucleic acid signal amplification methods.
Article
Chemistry, Applied
Zhiqiang Wang, Lujun Zhang, Jiajun Feng, Pengfei Tang, Shuaishuai Chen, Hanqing Yu, Yueyao Hu, Zhongkai Wang, Feng Jiang
Summary: This study designed a chitin-based macromolecular chain transfer agent (Chitin-CTA) to graft polymers onto chitin at the molecular level. Homogeneous reversible addition-fragmentation chain transfer (RAFT) polymerization was used to prepare chitin-graft-poly(methyl acrylate) (Chitin-gPMA) copolymers, which exhibited tunable glass transition temperatures. These branched MA elastomers showed unique strain-hardening behavior and significantly enhanced mechanical properties.
CARBOHYDRATE POLYMERS
(2022)
Article
Multidisciplinary Sciences
Chang Xue, Huimin Niu, Shuyao Hu, Zhe Yang, Lei Wang, Zai-Sheng Wu
Summary: This study develops a three-dimensional fluorescence counting method for accurately evaluating the expression level of microRNA-21 (miRNA-21) in individual HeLa cells. The results show that this method can locate and count target miRNAs in living cells, and the quantitative results are consistent with qPCR standard assay. By screening miR-21 expression, the proliferation and migration of HeLa cells can be evaluated, enabling the prediction of cancer metastasis in murine tumor models. In conclusion, this three-dimensional fluorescence counting method provides a valuable molecular tool for understanding the biological function of miRNAs and recognizing aggressive CTCs.
JOURNAL OF ADVANCED RESEARCH
(2023)
Article
Biophysics
Han -Wei Chu, Binesh Unnikrishnan, Amit Nain, Scott G. Harroun, Huan-Tsung Chang, Chih-Ching Huang
Summary: This study demonstrates the potential of using cluster ions generated from pulsed laser irradiation of aggregated nanoparticles in bioassays. By treating silver and gold nanoparticles with thrombin and analyzing the resulting clots, intense Ag-Au alloy cluster ions were used to detect thrombin concentration in human plasma, showing a dynamic range of 2.5-50 pM.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biophysics
Ren-Siang Wu, Yu-Syuan Lin, Amit Nain, Binesh Unnikrishnan, Yu-Feng Lin, Cheng-Ruei Yang, Tzu-Heng Chen, Yu-Fen Huang, Chih-Ching Huang, Huan-Tsung Chang
Summary: This study developed photostable carbon-dot liposomes (CDsomes)-based fluorophores for subcellular and suborganellar imaging, which can overcome the issues of easily photobleached commercial fluorophores. The CDsomes can selectively stain nucleolus, cytoplasm, and membrane in less than 1 minute. They offer high-contrast staining in both fixed and living cells and are more stable under photoirradiation. The CDsomes can be transferred to daughter cells for multigenerational tracking without interrupting the staining pattern.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biochemistry & Molecular Biology
Jing Yang, Jiamin Zou, Wei Zhong, Jin Zou, Yansha Gao, Shuwu Liu, Songbai Zhang, Limin Lu
Summary: A facile and sensitive electrochemical aptasensor based on Au nanoparticles-decorated porous carbon was developed for the efficient determination of the antibiotic drug chloramphenicol. The developed aptasensor exhibits a wide linear detection range and low detection limit, and can be used for the analysis of antibiotics in food samples.
Article
Chemistry, Physical
Anisha Anand, Hong-Jyuan Jian, Hao-Hsin Huang, Li Er Hean, Yu-Jia Li, Jui-Yang Lai, Hung-Da Chou, Yu-Chuan Kang, Wei-Chi Wu, Chi-Chun Lai, Chih-Ching Huang, Huan-Tsung Chang
Summary: In this study, anti-angiogenic carbon nanovesicles (CNVs) were synthesized for sustained release of anti-VEGF antibody to treat ocular neovascularization. The CNVs showed good loading efficiency, low cytotoxicity, and strong inhibitory effects on VEGF-induced cell proliferation and migration. In vivo studies demonstrated the superior efficacy of CNVs compared to bevacizumab alone, making them a promising therapeutic agent for age-related macular degeneration.
Article
Chemistry, Physical
Shih-Chun Wei, Amit Nain, Yu-Feng Lin, Ren-Siang Wu, Pavitra Srivastava, Lung Chang, Yu-Fen Huang, Huan-Tsung Chang, Kuan-Ting Chuang, Chih-Ching Huang
Summary: Bacterial infection in chronic wounds can be effectively treated using light-triggered carbon dot liposomes (CDsomes) to accelerate the healing process by targeting the inflammation and proliferation phase.
Article
Environmental Sciences
Naveed Ahmad, Chung-Feng Jeffrey Kuo, Mujahid Mustaqeem, Arumugam Sangili, Chih-Ching Huang, Huan-Tsung Chang
Summary: In order to address the presence of fluoroquinolone antibiotics in aquatic environments, it is necessary to design a semiconductor photocatalyst with excellent photocatalytic efficiency. This study explored the performance of MnNb2O6/g-C3N4 composites in the photocatalytic degradation of ciprofloxacin and tetracycline-HCl antibiotics. The enhanced photocatalytic activity of MNO/g-CN was found to be due to the electron's shifting between the MNO and g-CN sheets, promoting the formation of photo-generated e(-)/h(+) pairs, indicating the existence of a low-waste, high-performance material for eradicating ciprofloxacin and tetracycline-HCl from wastewater.
Article
Chemistry, Physical
Binesh Unnikrishnan, Chien -Wei Wu, Arumugam Sangili, Ya-Ju Hsu, Yu-Ting Tseng, Jyoti Shanker Pandey, Huan-Tsung Chang, Chih-Ching Huang
Summary: This article presents a simple fluorine-free exfoliation and two-step vulcanization method for synthesizing molybdenum sulfide-modified molybdenum carbide MXene, which exhibits excellent electrocatalytic hydrogen evolution performance in alkaline medium. The sulfidation method demonstrated in this study is capable of producing amorphous MoS2 structures on Mo2CTx MXene, enhancing stability and electrocatalytic activity.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Chemistry, Physical
Yu-Feng Lin, Yu-Syuan Lin, Tzu-Yun Huang, Shih-Chun Wei, Ren-Siang Wu, Chih-Ching Huang, Yu-Fen Huang, Huan-Tsung Chang
Summary: A nanozyme based on photoswitchable carbon-dot liposomes (CDsomes) was developed for programmable catalytic cascade reactions. The CDsomes can switch between a fluorescent on-state and nonfluorescent off-state under different wavelengths of light, enabling controlled generation of reactive oxygen species for tumor phototherapy.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Nanoscience & Nanotechnology
Hong-Jyuan Jian, Anisha Anand, Jui-Yang Lai, Binesh Unnikrishnan, Huan-Tsung Chang, Scott G. Harroun, Chih-Ching Huang
Summary: Effective treatment of infectious keratitis requires the elimination of pathogens, reduction of inflammation, and prevention of corneal damage. A nanocomposite (Arg-CQDs/pCur) consisting of arginine-derived carbon quantum dots (Arg-CQDs) and polymeric curcumin (pCur) was prepared. The Arg-CQDs/pCur nanocomposite showed combined antibacterial, antioxidative, anti-inflammatory, and pro-proliferative properties, making it an effective treatment for bacterial keratitis.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Engineering, Environmental
Wei -Yi Chu, Yi-Ru Chiou, Ren-Hao Luo, Tzu-Heng Chen, Cheng-Ju Yu, Yi-Ju Chou, Huan-Tsung Chang, Chien-Fu Chen
Summary: This article introduces a new technique for the synthesis of colloidosomes using a microfluidic droplet device and partially miscible liquids. By dissolving liquids within droplets, hydrophilic nanoparticles can be close-packed at the liquid interface to form colloidosomes. This method also enables colloidosomes to be synthesized within a narrow range of sizes. The close-packing of nanoparticles is confirmed by modifying colloidosomes into signal-enhanced lateral flow assays, which demonstrates their potential application in bacterial detection platforms, drug encapsulation, and biosensing.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Physical
Cheng-Ruei Yang, Yu-Syuan Lin, Ren-Siang Wu, Chin-Jung Lin, Han-Wei Chu, Chih-Ching Huang, Anisha Anand, Binesh Unnikrishnan, Huan-Tsung Chang
Summary: A ratiometric fluorescent probe based on o-phenylenediamine-derived carbonized polymer dots (o-PD CPDs) was developed for real-time intracellular detection of singlet oxygen (1O2). The o-PD CPDs can produce 1O2 and selectively quench the yellow fluorescence, allowing for the selective detection of 1O2 against other reactive oxygen species (ROS). The probe was successfully applied to monitor 1O2 level in the intracellular environment and detect 1O2 and other ROS species in an inflammatory neutrophil cell model.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Materials Science, Multidisciplinary
Yin-Chuan Chen, Yu-Feng Lin, Chun-Ting Liu, Yi-Chen Liu, Min-Hsuan Lin, Guan-Yu Lan, Yu-Shu Cheng, Hui-Ling Yu, Chih-Ching Huang, Huan-Tsung Chang, Nai-Chen Cheng, Yu-Sheng Hsiao, Jiashing Yu
Summary: Innovations in biomedical tissue engineering are increasing as researchers explore ways to develop biological scaffolds. The combination of the synthetic polymer PEDOT:PSS with the nanoconducting material MXene has been used to create conductive scaffolds for stem cell differentiation. These scaffolds not only influence cell behavior through their physical and biochemical properties, but also respond to external stimuli to alter cellular activities. The PEDOT:PSS/MXene scaffold has shown low cytotoxicity and promoted osteogenic differentiation of stem cells when used with electrical stimulation. This scaffold may serve as a platform for studying stem cell differentiation with various modes of electrical stimulation in a 3D environment.
ACS APPLIED POLYMER MATERIALS
(2023)
Article
Chemistry, Physical
Amit Nain, Yu-Ting Tseng, Akash Gupta, Yu-Feng Lin, Sangili Arumugam, Yu-Fen Huang, Chih-Ching Huang, Huan-Tsung Chang
Summary: We have developed multifunctional nanogels with antimicrobial, antioxidant, and anti-inflammatory properties, facilitating rapid wound healing. The nanogels, formed using quercetin and a carbonization process, possess excellent antioxidative and bacterial targeting properties. By incorporating copper sulfide nanoclusters, the nanogels show enhanced antimicrobial effects and can eradicate biofilms in diabetic mice wounds. The presence of the nanogels also regulates the expression of inflammatory and anti-inflammatory factors, promoting angiogenesis, epithelialization, and collagen synthesis for accelerated wound healing.
NANOSCALE HORIZONS
(2023)
Review
Chemistry, Analytical
Shihchun Wei, Yijia Li, Hsinhua Liang, Yaote Yen, Yihui Lin, Huantsung Chang
Summary: Carbon dots (C-dots) based photoluminescent (PL) assays have the potential to screen illicit drugs, and their advantages include stability and biocompatibility.
ANALYTICAL SCIENCES
(2022)