Article
Food Science & Technology
Mei Xie, Tianlan Chen, Xiong Xin, Zongwei Cai, Cheng Dong, Bo Lei
Summary: A method using a digital microfluidic chip and loop-mediated isothermal amplification technology was developed for multiplex detection of foodborne pathogens. The method showed higher sensitivity, simpler operation, higher efficiency, and smaller size compared to the commercial benchtop LAMP method.
Review
Plant Sciences
Sandra V. Gomez-Gutierrez, Stephen B. Goodwin
Summary: LAMP is a molecular technique for detection of plant pathogens, known for its specificity, sensitivity, and ability to conduct reactions at a constant temperature. This review explains the amplification process, visualization techniques, advantages, and disadvantages of LAMP compared to alternative isothermal approaches. It also presents the application of LAMP in detecting fungal pathogens and viruses in wheat, as well as the development of real-time and multiplex LAMP assays.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Food Science & Technology
Mengzhe Li, Zhixian Luan, Yongxiang Liu, Chen Yang, Yanling Wang, Cuiping Ma, Chao Shi
Summary: The study introduced an ultrafast bacterial cell lysis method induced by corona discharge, combined with loop-mediated isothermal amplification (LAMP) for rapid and convenient detection of foodborne pathogens. The corona discharge effectively destabilized bacterial cell envelopes and released more DNA, providing shorter preparation time and higher DNA yields compared to thermal lysis. Collaborating with colorimetric LAMP, the platform allowed visual detection of bacterial DNA in a time-efficient manner.
Article
Chemistry, Analytical
Han Jiang, Qian Wu, Qihong Zhao, Kaiyong Liu, Qingli Bo, Xinsheng Qin, Chao Yan, Lin Huang, Wei Chen, Panzhu Qin
Summary: Foodborne pathogens pose a serious threat to human health worldwide. In this study, a portable lateral flow colorimetric biosensor called CALL was developed using dCas9 as an intermediate bridge to distinguish target amplicons and byproducts. The biosensor showed high specificity and sensitivity in detecting Salmonella, enabling on-site screening of low concentrations in a short time.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Food Science & Technology
JunGang Du, Biao Ma, Jiali Li, Jiangbing Shuai, Xiaoping Yu, Xiaofeng Zhang, Mingzhou Zhang
Summary: The probe-based loop-mediated isothermal amplification is an efficient tool for the simultaneous detection of multiple bacterial pathogens in a short period of time. This method can identify three bacterial target fragments within 25 minutes and can be used for the detection of contaminated foods after enrichment.
FOOD ANALYTICAL METHODS
(2022)
Article
Chemistry, Multidisciplinary
Rajamanickam Sivakumar, So Yeon Park, Nae Yoon Lee
Summary: Here, a quercetin-mediated silver nanoparticle (AgNP) synthesis combined with loop-mediated isothermal amplification (LAMP) technique was used to detect SARS-CoV-2 and Enterococcus faecium using a foldable PMMA microdevice. The LAMP amplicons formed a complex with Ag+ ions, while the quercetin acted as a reducing agent and chelated with Ag+ ions, resulting in the formation of brown-colored AgNPs within 5 minutes. The method exhibited higher sensitivity than agarose gel electrophoresis and had a detection limit of 101 copies μL-1 for SARS-CoV-2 RNA and 101 CFU mL-1 for E. faecium.
Article
Biochemical Research Methods
Chunmei Xie, Shan Chen, Likun Zhang, Xiangpeng He, Yi Ma, Haiping Wu, Bingjie Zou, Guohua Zhou
Summary: A self-driven microfluidic chip was designed for multiplex detection of HBV, HCV, and HIV, achieving quick and efficient detection of target nucleic acids, especially suitable for resource-limited areas.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Huan-Huan Zhu, Yuan Li, Li-Xia Wu, Ke-Sheng Wang, Yue Zhang, Qiang-Yuan Fan, Zun-Zhen Ming, Wei-Qin Chen, Wei-Wei Liu
Summary: Loop-mediated isothermal amplification (LAMP) is a promising diagnostic tool for genetic amplification, known for its speed, simplicity, efficiency, and sensitivity. This research introduces an internal heating method for LAMP using magnetic nanoparticles (MNPs) called nano-LAMP, which can detect low concentrations of HPV and has been validated in clinical samples.
Review
Microbiology
Cassandra Suther, Sloane Stoufer, Yanjiao Zhou, Matthew D. Moore
Summary: Foodborne and enteric viruses impose a significant burden on public health and economy globally. Real time PCR, although considered a gold standard, has limitations in terms of portability, sample processing, and time to result. Isothermal amplification methods have shown promise as portable, sensitive, and rapid alternatives to PCR for detecting these viruses.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Food Science & Technology
Jie Zhou, Teng-Yu Wang, Zhi Lan, Han-Jie Yang, Xing-Jian Ye, Rui Min, Zhao-Hui Wang, Qing Huang, Jing Cao, Yu-E Gao, Wen-Long Wang, Xiu-Lan Sun, Yi Zhang
Summary: Foodborne microbial contamination (FMC) is the primary cause of food poisoning and illness. Isothermal amplification methods with functional nucleic acids (FNAs) have enabled the detection of mycotoxins, making biosensors for FMC more sensitive, cost-effective, and less reliant on nanomaterials for signal output.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Fisheries
Keshun Hu, Ye Li, Feng Wang, Jianying Liu, Yuanyuan Li, Qian Zhao, Xiaoye Zheng, Ningyu Zhu, Xiaoping Yu, Shaohua Fang, Jun Huang
Summary: In this study, a microfluidic chip detection system based on loop-mediated isothermal amplification (LAMP) was developed for the simultaneous detection of DIV1, WSSV, and EHP, which pose serious threats to shrimp farming. The system exhibited high sensitivity, specificity, and rapid detection, making it suitable for field and routine diagnoses in aquaculture.
JOURNAL OF FISH DISEASES
(2023)
Review
Chemistry, Analytical
Jesse M. M. Marangoni, Kenneth K. S. Ng, Arezoo Emadi
Summary: Loop-mediated isothermal amplification (LAMP) is a useful tool for pathogen detection in clinical and environmental samples, but monitoring LAMP can be challenging for point-of-use applications. This review discusses the use of voltammetric sensors for LAMP monitoring and highlights various strategies for detecting DNA amplicons, pyrophosphate, and protons. The review also addresses the hurdles in adapting these sensors for on-site applications.
Article
Plant Sciences
Xiayan Pan, Xiao Wang, Junjie Yu, Mina Yu, Huijuan Cao, Mingli Yong, Tianqiao Song, Zhongqiang Qi, Yan Du, Rongsheng Zhang, Yongfeng Liu
Summary: In this study, a rapid LAMP assay was developed to detect the mating type of Villosiclava virens, the causal agent of rice false smut. The LAMP assay showed high sensitivity and specificity, with the ability to detect small amounts of DNA and provide results in just 45 minutes. It can serve as a rapid testing tool for mating type recognition of V. virens isolates in the field.
Article
Chemistry, Analytical
Xiao Lu, Henson Lee Yu, Haosi Lin, Yumeng Cao, I-Ming Hsing
Summary: In this study, we developed a one-pot loop probe-mediated isothermal amplification scheme (oLAMP) that enables rapid and specific detection of pathogens in communicable diseases and food samples. This platform offers a scalable and reliable diagnostic pathway for decentralized detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Hanh An Nguyen, Heewon Choi, Nae Yoon Lee
Summary: In this study, a rotatable paper device integrating the loop-mediated isothermal amplification (RT-LAMP) technique and naked-eye readout was developed for the detection of infectious pathogens. The device utilized hydroxyl radicals to decolorize a food additive called carmoisine, and the color change of the solution indicated the presence of target DNA. The rotatable paper device successfully detected SARS-CoV-2, SARS-CoV, and Enterococcus faecium in milk within 70 minutes using only visual observation. The device offers a portable and low-cost tool for pathogen detection in resource-limited environments.
Article
Multidisciplinary Sciences
Youchuang Chao, Olinka Ramirez-Soto, Christian Bahr, Stefan Karpitschka
Summary: The research investigates the coupling between liquid-liquid phase separation and the spreading motion of three-phase contact lines, finding that the classical Cox-Voinov law is no longer applicable and observing spreading beginning well before any visible nucleation of microdroplets. The study suggests that evaporation-induced enrichment, together with surface forces, causes an earlier nucleation in the wetting precursor film, initiating the observed wetting transition.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Computer Science, Artificial Intelligence
Maolin Wang, Kelvin C. M. Lee, Bob M. F. Chung, Sharatchandra Varma Bogaraju, Ho-Cheung Ng, Justin S. J. Wong, Ho Cheung Shum, Kevin K. Tsia, Hayden Kwok-Hay So
Summary: Real-time in situ image analytics require stringent latency requirements on intelligent neural network inference operations. This study demonstrates that high-performance reconfigurable computing platforms based on FPGA processing can bridge the gap between low-level hardware processing and high-level intelligent image analytics algorithm deployment. The proposed design achieves ultralow classification latency with high accuracy for label-free classification of human PBMC subtypes.
IEEE TRANSACTIONS ON NEURAL NETWORKS AND LEARNING SYSTEMS
(2022)
Article
Chemistry, Multidisciplinary
Ruotong Zhang, Haisong Lin, Yi Pan, Chang Li, Zhenyu Yang, Jingxuan Tian, Ho Cheung Shum
Summary: This study comprehensively investigates the mechanisms of contact electrification in various liquid-liquid systems and demonstrates a liquid-liquid TENG for harvesting energy from rainwater. The liquid-liquid TENG has high charge density, high output stability, and long effective contact electrification time.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Chemistry, Applied
Yijie Wang, Xiufeng Li, Ting Li, Yang Wang, Jie Jiang, Xuhui Zhang, Jing Huang, Bihua Xia, Ho Cheung Shum, Zhenzhong Yang, Weifu Dong
Summary: A chemical approach is used to fabricate particles by combining a water-insoluble plant protein and a hydrophilic polysaccharide, resulting in adjustable interfacial wettability and improved absorption kinetics for emulsions.
FOOD HYDROCOLLOIDS
(2023)
Article
Chemistry, Multidisciplinary
Wei Guo, Danyang Ji, Andrew B. Kinghorn, Feipeng Chen, Yi Pan, Xiufeng Li, Qingchuan Li, Wilhelm T. S. Huck, Chun Kit Kwok, Ho Cheung Shum
Summary: RNA encodes interactions to modulate the properties of biomolecular condensates, and rG4s formed by guanine-rich sequences can trigger the formation of these condensates. The mechanisms by which rG4 motifs modulate phase transitions and material properties of condensates are unclear.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biochemical Research Methods
Fang Wang, Chang Li, Ruotong Zhang, Yuan Liu, Haisong Lin, Lang Nan, Muhammad Ajmal Khan, Yang Xiao, Ho Cheung Shum, Hui Deng
Summary: Cold atmospheric plasma chip enables targeted and multi-dosage treatment of multiple cell lines in a single session. This innovative approach provides a new tool for investigating the mechanisms of plasma-induced anticancer effects and guiding clinical applications.
Article
Oncology
Minda Zhang, Zeyu Shi, Shuaishuai Zhang, Xudan Li, Sally Kit Yan To, Yijia Peng, Jie Liu, Siming Chen, Hongyu Hu, Alice Sze Tsai Wong, Jin-Zhang Zeng
Summary: English Summary: Compound K (CK) derived from ginseng binds and modulates Nur77 phosphorylation to inhibit cancer stem-cell-like cells (CSCs) and suppress colorectal cancer (CRC) metastasis. CK disrupts the Nur77-Akt feed-forward loop, inactivates PI3K/Akt, and reactivates the silenced Dicer transcription under a hypoxic microenvironment. CK shows potential as a nutraceutical against CRC and offers an alternative strategy for PI3K/Akt inactivation by targeting Nur77, overcoming concerns aboutdrug selectivity and safety.
Article
Chemistry, Multidisciplinary
Shi Yang, Han Yu, Xiuli Xu, Ting Yang, Yue Wei, Rui Zan, Xiaonong Zhang, Qingming Ma, Ho Cheung Shum, Yang Song
Summary: This article presents the synthesis of a peptide-based RNA sensor by connecting aggregation-induced emission luminogen (AIEgen) tetraphenylethylene (TPE) to tandem peptide repeats of (RRASL)n (n = 1, 2, 3). The conjugation of TPE promotes liquid-liquid phase separation of the peptide repeats, with significantly decreased minimum coacervation concentration (MCC) compared to the untagged counterparts. Furthermore, the luminescence of TPE-(RRASL)n is greatly enhanced with increasing RNA concentration, attributed to the constricted rotation of the TPE moiety within the droplet phase formed by peptide/RNA coacervates. In addition, TPE-(RRASL)n can efficiently penetrate human gallbladder carcinoma cells, translocate into the nucleus, and colocalize with intracellular RNA, highlighting its potential as a droplet-based biosensor for intracellular RNA imaging.
Article
Chemistry, Multidisciplinary
Chang Li, Yafeng Yu, Huizeng Li, Haisong Lin, Huanqing Cui, Yi Pan, Ruotong Zhang, Yanlin Song, Ho Cheung Shum
Summary: The heterogeneous self-assembly of a single type of nanoparticle is achieved by quickly evaporating a colloid-poly(ethylene glycol) (PEG) droplet, facilitated by the spatial confinement induced by a skin layer. This approach allows for the formation of face-centered-cubic (FCC) lattices with different plane orientations, generating binary bandgaps and structural colors. The self-assembly of nanoparticles can be regulated by adjusting the PEG concentration, and the method is applicable to various droplet shapes, substrates, and nanoparticle types.
Article
Chemistry, Multidisciplinary
Yanting Shen, Yuan Liu, Janine K. Nunes, Chenmin Wang, Miao Xu, Michael K. T. To, Howard A. Stone, Ho Cheung Shum
Summary: Injectable fibro-gel, a water-filled network of entangled hydrogel microfibers, has shown great potential in tissue engineering and regenerative medicine due to its injectability, tailorable physicochemical properties, and controlled drug release profiles. Preliminary in vivo tests on a mice excision skin model have demonstrated that the fibro-gel promotes faster wound healing and new tissue regeneration compared to a commercial gel. Moreover, the release of distinct drugs at different rates using a two-layer fibro-gel model further enhances wound healing efficiency.
ADVANCED MATERIALS
(2023)
Article
Multidisciplinary Sciences
Zehao Pan, Janine K. Nunes, Camille Duprat, Ho Cheung Shum, Howard A. Stone
Summary: When a suspension of particles passes through a constriction, the particle volume fraction either decreases or remains unchanged. However, an entangled fiber suspension can increase its volume fraction by over 10 times after passing through a constriction. This increase is attributed to the entanglements among the fibers, which allow the network to move faster than the liquid. This finding provides a new strategy to control soft material properties during delivery processes in healthcare, 3D printing, and material repair.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Feipeng Chen, Xiufeng Li, Yafeng Yu, Qingchuan Li, Haisong Lin, Lizhi Xu, Ho Cheung Shum
Summary: The authors present a one-step fabrication method based on aqueous phase separation to construct two-aqueous-phase gels with multiple phases. These gels exhibit enhanced interfacial mechanics and can be conveniently constructed with programmable structures and tunable properties. The versatility of this approach is demonstrated by mimicking various biological architecture features at different length scales.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Yi Pan, Chang Li, Xiaoyu Hou, Zhenyu Yang, Mingzhu Li, Ho Cheung Shum
Summary: The challenge of achieving individually and independently programmable stimuli-responsive color pixels in color-changing soft materials and devices has been addressed by designing a morphable concavity array on a two-dimensional photonic crystal elastomer. By using multichannel microfluidics, the colors of each concavity can be switched controllably, enabling dynamic display for applications such as anti-counterfeiting and encryption.
Review
Chemistry, Multidisciplinary
Yan Zhan, Chang Li, Zhigang Che, Ho Cheung Shum, Xiaotian Hu, Huizeng Li
Summary: Benefiting from their superior photophysical properties and low-cost crystalline substances, perovskites have emerged as competitive photoactive semiconductors for optoelectronic devices. Photon management is crucial for perovskites due to their direct band gap and high refractive index. Photonic structures with periodic features offer efficient means to manage light through the interaction between media and photons. This review discusses strategies of photon management in perovskite optoelectronics and summarizes the design and implementation progress of photonic elements for high-index perovskites. Proposals and guidelines for high-performance multifunctional perovskite optoelectronics are also provided.
ENERGY & ENVIRONMENTAL SCIENCE
(2023)
Article
Chemistry, Physical
Niki Abbasi, Janine K. Nunes, Zehao Pan, Tejas Dethe, Ho Cheung Shum, Andrej Kosmrlj, Howard A. Stone
Summary: Liquid-liquid phase separation induced by co-flow of a nonequilibrated aqueous two-phase system within a planar flow-focusing microfluidic device is experimentally demonstrated. Invasion fronts from the outer stream are formed along the top and bottom walls of the microfluidic device, which merge as the system reaches steady-state. The formation and growth of the invasion fronts are driven by Marangoni flow induced by the polymer concentration gradient along the width of the channel during phase separation.
