Review
Immunology
Antonio Marinho, Jose Delgado Alves, Jorge Fortuna, Raquel Faria, Isabel Almeida, Gloria Alves, Joao Araujo Correia, Ana Campar, Mariana Brandao, Jorge Crespo, Daniela Marado, Joao Matos-Costa, Susana Oliveira, Fernando Salvador, Lelita Santos, Fatima Silva, Milene Fernandes, Carlos Vasconcelos
Summary: "Evidence and practice-based guidance for off-label use of biologics in SLE, APS, and SS: a comprehensive literature review and expert consensus resulted in 32 final recommendations, considering organ involvement, manifestations, severity, and response to previous treatments."
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Rheumatology
Sherri Longobardi, Charmaine Lopez-Davis, Bhuwan Khatri, Constantin Georgescu, Cherilyn Pritchett-Frazee, Christina Lawrence, Astrid Rasmussen, Lida Radfar, Robert Hal Scofield, Alan N. Baer, Susan A. Robinson, Erika Darrah, Robert C. Axtell, Gabriel Pardo, Jonathan D. Wren, Kristi A. Koelsch, Joel M. Guthridge, Judith A. James, Christopher J. Lessard, Amy Darise Farris
Summary: This study identified autoantibodies in anti-Ro/SS-A negative primary Sjogren's syndrome (SS), providing a new approach for diagnosing Ro-negative SS.
ANNALS OF THE RHEUMATIC DISEASES
(2023)
Article
Pediatrics
Jing Yin, Jijun Ma, Jingyue Xia, Yang Cao, Chongwei Li
Summary: This study reports a case of APDS1 with a unique clinical phenotype presenting as SLE and SS-like features. The patient achieved remission after treatment with medication.
FRONTIERS IN PEDIATRICS
(2022)
Article
Medicine, General & Internal
Yan Han, Ziyi Jin, Ling Ma, Dandan Wang, Yun Zhu, Shanshan Chen, Bingzhu Hua, Hong Wang, Xuebing Feng
Summary: This study explores the clinical features of patients with systemic lupus erythematosus and Sjogren's syndrome overlap and develops predictive tools for screening SLE-SS cases.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Dermatology
Yukina Watanabe, Marumi Saitou, Toshiyuki Yamamoto
Summary: This case report describes a rare case of lupus miliaris disseminatus faciei on the chin and neck in a patient with systemic lupus erythematosus and Sjogren's syndrome. The study suggests that activated M2 macrophages may play a role in the induction of the condition.
JOURNAL OF DERMATOLOGY
(2021)
Article
Rheumatology
Robin Arcani, Daniel Bertin, Nathalie Bardin, Karin Mazodier, Rodolphe Jean, Pierre Suchon, Geoffroy Venton, Aurelie Daumas, Estelle Jean, Patrick Villani, Gilles Kaplanski, Pierre-Andre Jarrot
Summary: The study shows that anti-NuMA antibodies are mainly associated with pSS and SLE, and patients with these antibodies have less severe clinical profiles and require less treatment. Anti-NuMA antibodies may be a useful diagnostic marker when other auto-antibodies are negative and could indicate a good prognosis in autoimmune diseases.
Review
Biochemistry & Molecular Biology
Wojciech Lubon, Malgorzata Lubon, Przemyslaw Kotyla, Ewa Mrukwa-Kominek
Summary: Systemic lupus erythematosus (SLE) is a chronic multisystem autoimmune disease, with up to one-third of patients experiencing various ocular manifestations. Ocular findings may be the initial signs of the disease and can lead to severe complications and vision loss. Early diagnosis and proper management are crucial, requiring collaboration among specialists. Adequate preparation of ophthalmologists can help differentiate SLE complications from other eye disorders. New therapies for SLE show promise, but their ocular side effects are still unknown.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Rheumatology
Faria Sami, Shahzad Ahmed Sami, Augustine M. Manadan, Shilpa Arora
Summary: In this study, we investigated the occurrence of NMO in SLE and SS patients and compared the clinical characteristics and outcomes of different groups of NMO hospitalizations. The results showed that NMO patients have a higher risk of SLE and Sjogren's disease, especially in women and certain ethnic groups. These patients have a higher comorbidity index, more complications, longer hospital stays, and higher healthcare costs, but overall mortality rates did not differ significantly.
CLINICAL RHEUMATOLOGY
(2023)
Article
Otorhinolaryngology
Il Hwan Lee, Dong-Kyu Kim
Summary: This study found a significant association between chronic rhinosinusitis (CRS) and an increased incidence of Sjagren's syndrome, while no association was found with systemic lupus erythematosus or ankylosing spondylitis.
Article
Rheumatology
Antonis Fanouriakis, Myrto Kostopoulou, Jeanette Andersen, Martin Aringer, Laurent Arnaud, Sang-Cheol Bae, John Boletis, Ian N. Bruce, Ricard Cervera, Andrea Doria, Thomas Doerner, Richard A. Furie, Dafna D. Gladman, Frederic A. Houssiau, Luis Sousa Ines, David Jayne, Marios Kouloumas, Laszlo Kovacs, Chi Chiu Mok, Eric F. Morand, Gabriella Moroni, Marta Mosca, Johanna Mucke, Chetan B. Mukhtyar, Gyoergy Nagy, Sandra Navarra, Ioannis Parodis, Jose M. Pego-Reigosa, Michelle Petri, Bernardo A. Pons-Estel, Matthias Schneider, Josef S. Smolen, Elisabet Svenungsson, Yoshiya Tanaka, Maria G. Tektonidou, Y. K. Onno Teng, Angela Tincani, Edward M. Vital, Ronald F. van Vollenhoven, Chris Wincup, George Bertsias, Dimitrios T. Boumpas
Summary: The EULAR recommendations for the management of systemic lupus erythematosus (SLE) have been updated based on emerging new evidence. The updated recommendations provide consensus guidance on the use of various medications and treatment strategies to control the disease, as well as recommendations for assessing treatment response and tapering therapy.
ANNALS OF THE RHEUMATIC DISEASES
(2023)
Article
Rheumatology
Jingru Tian, Dingyao Zhang, Xu Yao, Yaqing Huang, Qianjin Lu
Summary: This study estimated the epidemiology of SLE globally, finding variations in incidence and prevalence rates across different countries and regions. It also highlighted the impact of gender, age, and prevalence estimation method on the findings. SLE was found to occur more frequently in high-income countries.
ANNALS OF THE RHEUMATIC DISEASES
(2023)
Article
Immunology
Jessica S. Kleer, Pascal A. Rabatscher, Jessica Weiss, Joel Leonardi, Severin B. Vogt, Andrea Kieninger-Grafitsch, Carlo Chizzolini, Uyen Huynh-Do, Camillo Ribi, Marten Trendelenburg
Summary: The study found that epitope-specific anti-C1q in SLE patients is associated with specific disease manifestations, providing more diagnostic value than conventional anti-C1q.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Immunology
Mingxuan Chi, Kuai Ma, Yunlong Li, Min Quan, Zhongyu Han, Zhaolun Ding, Xin Liang, Qinxiu Zhang, Linjiang Song, Chi Liu
Summary: Systemic lupus erythematosus (SLE) is a typical autoimmune disease characterized by multiple immunoregulatory abnormalities that lead to damage to various tissues and organs. Researchers have identified aberrant expression of miRNAs in SLE patients, which may be associated with abnormal immune responses and disease progression in SLE.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Rheumatology
Bong-Woo Lee, Eui-Jong Kwon, Youngjae Park, Jennifer Jooha Lee, Ji Hyeon Ju, Sung-Hwan Park, Seung-Ki Kwok
Summary: This study found that shorter disease duration and higher disease activity of Sjogren's syndrome (SS) at baseline may be risk factors for future development of systemic lupus erythematosus (SLE). Serologic predictors of SLE development in SS patients include hypocomplementemia, leukopenia, thrombocytopenia, and positivity for anti-dsDNA, anti-RNP, and anti-ribosomal P antibodies.
Article
Medicine, General & Internal
Hsin-Hua Chen, Ching-Heng Lin, Wen-Cheng Chao
Summary: The study revealed a strong association between APS and the risk of newly diagnosed SLE, with APS patients having a significantly higher risk of developing SLE within 5 years compared to non-APS individuals. This association remained robust even after propensity score matching, suggesting the need for close monitoring and early detection of SLE symptoms in patients with APS.
FRONTIERS IN MEDICINE
(2021)
Article
Biochemical Research Methods
Anca Raluca Muresan, Khandoker Asiqur Rahaman, Junghyun Son, Min-Jung Kang, Oh-Seung Kwon
Summary: This study evaluated the metabolism of bolasterone in rats through in vitro and in vivo experiments. Sixteen hydroxylated metabolites and two other metabolites were detected and their structures and characteristic ions were identified. This information can be useful for identifying similar banned substances in doping screening tests.
DRUG TESTING AND ANALYSIS
(2023)
Article
Chemistry, Medicinal
Laura Goracci, Alessandra Nurisso, Emile Roussel, Basile Peres, Vincent Chaptal, Pierre Falson, Christelle Marminon, Joachim Jose, Marc Le Borgne, Ahcene Boumendjel
Summary: Human breast cancer resistance protein (BCRP), also known as ABCG2, plays a crucial role in tumor cells' resistance to multiple drugs. In this study, the researchers used computer-aided drug design (CADD) to guide the design of effective ABCG2 inhibitors. The FLAPpharm method was employed to understand the pharmacological features of one of the ABCG2 binding sites involved in regulating the transporter's ATPase activity. The model was evaluated using three predicted compounds, which showed high inhibitory activity against BCRP in the nanomolar range for the best compound.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Hong-Rae Kim, Ji-Hong Bong, Min-Jung Kang, Won-Bo Shim, Jeong-Soo Lee, Jae-Chul Pyun
Summary: This article presents a one-step immunoassay based on filtration, using microbeads for target analyte detection and filters with appropriate pore sizes. The optimal concentrations of urease and antibody were determined for effective bacterial detection, and the pH change was measured using a FET. Antibodies against target bacteria were isolated from horse serum by filtration and showed high specificity in overcoming cross-reactivity. The FET-based one-step immunoassay was successfully demonstrated for five types of food poisoning-related bacteria in human serum.
Article
Chemistry, Medicinal
Magdalena N. Wojciechowski, Sebastian Schreiber, Joachim Jose
Summary: This study presents a ligand-binding approach using surface-displayed HCN4 C-Linker-CNBD on E. coli to monitor the binding of 8-Fluo-cAMP. The binding affinity and mode were determined, and a new HCN4 channel-targeting ligand was identified.
Article
Biophysics
Moon-Ju Kim, Hyung Eun Bae, Soonil Kwon, Mi-Kyung Park, Dongeun Yong, Min-Jung Kang, Jae-Chul Pyun
Summary: The use of phages as a therapeutic strategy for multidrug-resistant bacterial infections requires the isolation and detection of phages from the environment. This study proposes a nanoplasmonic-based biodetection platform that utilizes bacterial outer membranes as a biorecognition element. By using a safe biochemical outer membrane fraction presenting phage-specific receptors, the robust and reliable detection of phages is achieved.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biophysics
Jaeyong Jung, Ji-Hong Bong, Jeong Soo Sung, Jun-Hee Park, Tae-Hun Kim, Soonil Kwon, Min-Jung Kang, Joachim Jose, Jae-Chul Pyun
Summary: The detection of SARS-CoV-1 was achieved using SPR biosensor and impedance spectrometry with screened Fv-antibodies. Two Fv-variants (clones) with specific affinity to SARS-CoV-1 were identified and named Anti-SP1 and Anti-SP2. The binding affinities of these Fv-variants were analyzed and their binding constants (KD) were estimated. The expressed Fv-antibodies, including fusion proteins with GFP, were also tested for their binding affinities to the SP. The feasibility of detecting SARS-CoV-1 using immobilized Fv-antibodies was demonstrated.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biochemistry & Molecular Biology
Anna Nickelsen, Claudia Goetz, Florian Lenz, Karsten Niefind, Simone Koenig, Joachim Jose
Summary: This study identified 38 new interaction partners of human CK2β, including HSP70-1, using photo-crosslinking and mass spectrometry. The interaction between HSP70-1 and CK2β was determined to have a K-D value of 0.57 μM. Phosphorylation studies suggested that HSP70-1 does not function as a substrate or activity modulator of CK2, indicating an independent interaction of HSP70-1 with CK2β.
Article
Chemistry, Multidisciplinary
Priscila Lopez-Rojas, Samer Haidar, Franziska M. Juergens, Dagmar Aichele, Angel Amesty, Ana Estevez-Braun, Joachim Jose
Summary: In this study, the inhibitory activity of endocrocin and its derivatives on human protein kinase CK2 was evaluated. Endocrocin was found to be a moderate inhibitor, while only one derivative showed strong inhibitory activity. Furthermore, the effects of emodin, endocrocin, and compound 4i on breast cancer cells (MCF7) were investigated.
RESULTS IN CHEMISTRY
(2023)
Article
Chemistry, Analytical
Jun-Hee Park, Ga-Yeon Lee, Zhiquan Song, Ji-Hong Bong, Hong-Rae Kim, Min-Jung Kang, Jae-Chul Pyun
Summary: An electrochemical immunoassay method based on redox cycling was developed using vertically paired electrodes (VPEs) made of PEDOT:PSS as an electrode material and parylene-C as a dielectric layer. Various electrochemical properties of PEDOT:PSS were analyzed for the redox reaction of TMB at different pH conditions, and the factors affecting the sensitivity of redox cycling based on VPEs were investigated. Computer simulation was performed to explore the fabrication limitations of VPEs, and the redox cycling method was successfully applied to the medical diagnosis of hHCV. The sensitivity of the redox cycling method was compared with other conventional assay methods.
Article
Nanoscience & Nanotechnology
Jun-Hee Park, Hong-Rae Kim, Moon-Ju Kim, Zhiquan Song, Min-Jung Kang, Dong Hee Son, Jae-Chul Pyun
Summary: In this study, a defect-passivated photosensor based on cesium lead bromide perovskite quantum dots was successfully fabricated and applied for microbial detection. The parylene film used for physical protection and defect passivation of the perovskite QD showed significant improvement in sensor performance.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Katharina Possart, Fabian C. Herrmann, Joachim Jose, Thomas J. Schmidt
Summary: This article investigates the drug treatment of tropical diseases caused by parasites. Through enzyme analysis and computational screening, several compounds with dual inhibitory effects were identified, which could potentially be used for the development of new drugs.
Article
Biophysics
Moon-Ju Kim, Zhiquan Song, Tae Gyeong Yun, Min-Jung Kang, Dong Hee Son, Jae-Chul Pyun
Summary: This study presents an air-permeable fabric-based ZnO nanogenerator for wearable piezoelectric sensors, allowing reliable monitoring of biomechanical activities through the skin. The sensors are capable of collecting various physiological information, such as pulse, breathing, saliva swallowing, and coughing, providing real-time monitoring of health-related vital signs without disrupting daily lives.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biochemistry & Molecular Biology
Joyeta Mahmud, Hien Thi My Ong, Eda Ates, Hong Seog Seo, Min-Jung Kang
Summary: This study investigated the secretome protein profile of induced-hypertrophy cardiomyocytes to identify next-generation biomarkers for AMI diagnosis and management. The results showed that 14-3-3 protein-zeta played an important role in cardiac hypertrophy and cardiovascular disorders and had the potential to be a novel biomarker and therapeutic strategy.
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)
