Article
Orthopedics
Cheng Li, Hao Li, Xue Yang, Fang-Zheng Zhu, Chi Xu, Andrej Trampuz
Summary: This study aimed to assess the diagnostic performance of synovial fluid polymerase chain reaction (PCR) in periprosthetic hip and knee infection and determine its diagnostic significance compared to conventional methods. The results showed that synovial fluid PCR has a high sensitivity and specificity in the rapid diagnosis of periprosthetic joint infection and early-stage culture-negative bacterial infections.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2022)
Article
Rheumatology
Jean-David Albert, Maena Le Corvec, Olivia Berthoud, Claire David, Xavier Guennoc, Emmanuel Hoppe, Sandrine Jousse-Joulin, Benoit Le Goff, Hugues Tariel, Olivier Sire, Anne Jolivet-Gougeon, Guillaume Coiffier, Olivier Loreal
Summary: The study demonstrates that mid-infrared fibre evanescent wave spectroscopy can effectively differentiate septic from non-septic synovial fluids in a multicentre cohort, and is particularly useful in ruling out septic arthritis.
Article
Infectious Diseases
Takashi Imagama, Kazushige Seki, Toshihiro Seki, Atsunori Tokushige, Yuta Matsuki, Kazuhiro Yamazaki, Daisuke Nakashima, Tomoya Okazaki, Kenji Hirata, Manabu Yamamoto, Hiroshi Tanaka, Takashi Sakai
Summary: This study found that measuring synovial fluid presepsin is reliable for early diagnosis of NJSA, and can be used as a novel biomarker for differentiating between NJSA and CA.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Biochemistry & Molecular Biology
Eun-Yeong Bok, Saet-Byul Kim, Gitika Thakur, Yong-Ho Choe, Seong-Ju Oh, Sun-Chul Hwang, Sun-A. Ock, Gyu-Jin Rho, Sang-Il Lee, Won-Jae Lee, Sung-Lim Lee
Summary: This study evaluated the stability of reference genes (RGs) in mesenchymal stem cells (MSCs) derived from rheumatoid arthritis patients. The results showed that TBP, PPIA, and YWHAZ are the most stable RGs for normalizing MSCs expression, regardless of exposure to inflammatory cytokines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Ophthalmology
Satoko Nakano, Yasuhiro Tomaru, Toshiaki Kubota, Hiroshi Takase, Manabu Mochizuki, Norio Shimizu, Sunao Sugita
Summary: The Direct Strip PCR test offers rapid and accurate detection of infectious pathogens causing uveitis. It shows high concordance with qPCR results, even when used by beginners, indicating its reliability for clinical use. Additionally, it can be used for intraoperative diagnosis, improving the prognosis of infectious diseases.
Article
Biochemical Research Methods
Weidu Song, Chuanhao Zhang, Huichao Lin, Taiyi Zhang, Haixia Liu, Xiaowen Huang
Summary: The proposed real-time rotary PCR reaction system (RRP) meets the requirements of low cost, low-power consumption, potential for miniaturization, and portability. Experimental and theoretical results demonstrate its feasibility in the rapid quantitative detection of Pseudomonas aeruginosa and have broad application prospects in bacteria detection and food safety.
Article
Biochemical Research Methods
Shaolei Huang, Yiquan An, Bangchao Xi, Xianglian Gong, Zhongfu Chen, Shan Shao, Shengxiang Ge, Jun Zhang, Dongxu Zhang, Ningshao Xia
Summary: Nucleic acid detection is a method that directly identifies the presence of pathogenic microorganisms and has advantages of high sensitivity, commendable specificity, and a short window period. A real-time PCR system based on multiple temperature zones was proposed to solve the time-consuming problem, enabling fast detection speed and high sensitivity under extreme conditions.
Article
Biochemistry & Molecular Biology
Layth Ben-Trad, Constantin Ionut Matei, Mirela Maria Sava, Samira Filali, Marie-Eve Duclos, Yves Berthier, Michel Guichardant, Nathalie Bernoud-Hubac, Ofelia Maniti, Ahmed Landoulsi, Marie-Genevieve Blanchin, Pierre Miossec, Thierry Granjon, Ana-Maria Trunfio-Sfarghiu
Summary: This study compares the biochemical, tribological, and ultrastructural properties of synovial fluid in healthy individuals and patients with osteoarthritis and arthritis, finding differences that indicate the importance of synovial fluid in joint mechanics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Jae Jong Kim, Hyoung-Min Park, A. Young Kyoung, In Kyung Park, Si-Kyu Lim, Byoung Chul Park
Summary: Genetic mutations like SNP are common in genetic disorders and cancers, and PCR methods are widely used for SNP detection. The specifically designed PCR platform STexS can efficiently distinguish small amounts of SNP mixed in normal DNA, aiding in targeting potential SNPs or genetically mutated biomarkers in human clinical samples.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Ardian Ramadani, Benoit Coulin, Giacomo De Marco, Oscar Vazquez, Anne Tabard-Fougere, Nathaly Gavira, Christina N. Steiger, Romain Dayer, Dimitri Ceroni
Summary: This study aimed to report on the clinical course, biological parameters, and results of microbiological investigations among children with K. kingae-induced septic arthritis of the knee. The Kocher-Caird prediction algorithm is not sensitive enough to effectively detect K. kingae-induced septic arthritis of the knee, and nucleic acid amplification assays on oropharyngeal swabs and joint fluid are necessary to exclude this condition in young children presenting with knee effusion.
PEDIATRIC INFECTIOUS DISEASE JOURNAL
(2023)
Article
Rheumatology
Guillaume Coiffier, Olivia Berthoud, Jean David Albert, Francois Robin, Claire Goussault, Anne Gougeon-Jolivet, Claude Bendavid, Pascal Guggenbuhl
Summary: RESAS is a new composite score of four SF variables with excellent performance to predict SA in the acute arthritis population.
Article
Veterinary Sciences
Alexandra Vilen, Bo Nilson, Ann-Cathrine Petersson, Mariana Cigut, Christel Nielsen, Henriette Strom
Summary: The study demonstrated that the sensitivity of 16S rRNA PCR was lower than bacterial culture for dogs with clinical SA, and there is currently no clear advantage in using 16S rRNA PCR as a diagnostic tool for these dogs. Furthermore, the study suggested that pre-incubation in paediatric blood bacterial culture bottles before bacterial cultivation on blood agar plates might enhance bacterial culture sensitivity compared to other methods.
ACTA VETERINARIA SCANDINAVICA
(2021)
Article
Biochemistry & Molecular Biology
Chiara Baggio, Roberto Luisetto, Carlotta Boscaro, Anna Scanu, Roberta Ramonda, Mattia Albiero, Paolo Sfriso, Francesca Oliviero
Summary: Genomic damage is associated with autoimmune processes, chronic inflammation, and apoptosis. The T cell compartment may exhibit overall genomic instability in certain rheumatological diseases. However, there is limited knowledge regarding leukocyte abnormalities in synovial fluid (SF) and their connection to inflammation. This study aimed to investigate cellular phenotypes in SF from patients with various inflammatory arthropathies. The results showed that inflammatory SF is associated with genomic instability and abnormal cell subsets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Infectious Diseases
Anna Maria Peri, Patrick N. A. Harris, David L. Paterson
Summary: This article reviews currently available emerging assays for the diagnosis of bloodstream infections directly from whole blood, including their performance and the available data about their impact on patient outcome.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Multidisciplinary Sciences
Aongart Mahittikorn, Frederick Ramirez Masangkay, Kwuntida Uthaisar Kotepui, Giovanni De Jesus Milanez, Manas Kotepui
Summary: This study compared the performance of PCR using DNA extracted from dried blood spots (DBS) and whole blood for detecting malarial parasites. While no significant difference was found in overall performance between DBS-extracted DNA and whole blood-extracted DNA, a lower accuracy in detecting Plasmodium vivax with DBS-extracted DNA was noted. Care should be taken when detecting P. vivax in endemic areas using PCR with DNA obtained from DBS. Further studies are needed to investigate the performance of PCR using DBS for detecting P. vivax and other malarial parasites.
SCIENTIFIC REPORTS
(2021)
Article
Pediatrics
James D. Sires, Nicole Williams, Shyamala C. Huilgol, Isaac Harvey, Georgia Antoniou, Joseph Dawson
PEDIATRIC SURGERY INTERNATIONAL
(2020)
Article
Orthopedics
Fui Lin Wong, Georgia Antoniou, Nicole Williams, Peter J. Cundy
JOURNAL OF CHILDRENS ORTHOPAEDICS
(2020)
Editorial Material
Surgery
Nicole Williams, Jackie Winters, Rebecca Cooksey
ANZ JOURNAL OF SURGERY
(2020)
Article
Pediatrics
Elias Awwad, Morgan Tolley, Georgia Antoniou, Nicole Williams
Summary: This study aimed to highlight the varied presentations of Kingella kingae musculoskeletal infections in children. Most cases involved the lower limbs, especially the knee joint. Most patients presented with mild fever, slightly elevated inflammatory markers, but blood culture results were often negative.
JOURNAL OF PAEDIATRICS AND CHILD HEALTH
(2021)
Article
Surgery
James Sires, Christopher Ovenden, Georgia Antoniou, Nicola Robinson, Nicole Williams
Summary: A change in hospital guidelines did not significantly reduce the use of CT scans or eliminate unnecessary CT scans in paediatric neck injuries. Unnecessary CT scans were a result of lack of knowledge and compliance with the guidelines. Individual hospitals should adopt strategies to reduce unnecessary CT scans in children, as it is associated with an increased risk of cancer.
ANZ JOURNAL OF SURGERY
(2022)
Review
Pediatrics
Emily Marriott, Sarah Twomey, Michael Lee, Nicole Williams
Summary: The study found variability in Australian screening guidelines for developmental dysplasia of the hip, including recommendations for examiner, screening methods, examination timing, and referral advice. Lack of knowledge regarding local screening guidelines may contribute to an increase in late diagnosed cases of DDH in Australia.
JOURNAL OF PAEDIATRICS AND CHILD HEALTH
(2021)
Article
Surgery
Belinda Balhatchet, Heike Schutze, Anum Awais, Nicole Williams
Summary: This study examined the publication rate and reasons for non-publication of abstracts presented at the Australian Orthopaedic Association Annual Scientific Meeting (AOA ASM) between 2012 and 2015. The overall publication rate was found to be 51%, higher than many similar Australian meetings and on par with other international orthopaedic and subspecialty meetings. Common reasons for non-publication included lack of time, publication considered low priority, and journal rejections.
ANZ JOURNAL OF SURGERY
(2022)
Review
Surgery
Samuel Abbot, Nicole Williams
Summary: The study reviewed 37 articles related to LSDs, with most focusing on surgical management of craniocervical manifestations. Some articles proposed prophylactic surgery. Additionally, surgical indications and techniques for thoracolumbar pathology were discussed, with recommendations for combined anterior and posterior approaches.
ANZ JOURNAL OF SURGERY
(2022)
Review
Medicine, General & Internal
Belinda Balhatchet, Heike Schutze, Nicole Williams, Bruce Ashford
Summary: This review aims to synthesize the current knowledge on factors that impact burnout and psychological wellbeing in Australian postgraduate medical trainees, filling the research gap specific to this population and providing a foundation for future studies.
SYSTEMATIC REVIEWS
(2021)
Review
Nursing
Larissa Smart, Lemuel Pelentsov, Jessie Childs, Nicole Williams, Adrian Esterman
Summary: This review discusses the physical assessments used by nurses in the screening of developmental hip dysplasia and highlights the need for further research.
JOURNAL OF CHILD HEALTH CARE
(2022)
Article
Surgery
Jonghoo Sung, Angela Yao, Georgia Antoniou, Rebecca Cooksey, Jacqueline Winters, Michael Ee, Nicole Williams
Summary: This study aimed to evaluate factors contributing to the failure of trauma team activation in the management of pediatric trauma. The results showed that factors such as abdominal and/or pelvic injuries, non-accidental injuries, and arrival via emergency medical retrieval service were predictors of no trauma team activation. It is important to carefully clarify the mechanism of injury and potential occult injuries even in patients transferred to the Pediatric Intensive Care Unit (PICU) or High Dependency Unit (HDU) to ensure appropriate trauma team activation.
ANZ JOURNAL OF SURGERY
(2022)
Review
Health Care Sciences & Services
Brett Ritchie, Kylie Porritt, Tania Marin, Nicole Williams
Summary: This review aimed to compare the diagnostic test accuracy of serum procalcitonin and serum C-reactive protein for suspected osteomyelitis and septic arthritis in hospitalized children and adolescents. The results showed that C-reactive protein is a more accurate and reliable inflammatory marker, while further research is needed to determine the diagnostic accuracy of procalcitonin.
JBI EVIDENCE SYNTHESIS
(2021)
Article
Medicine, General & Internal
Gajan Selvakumaran, Nicole Williams
AUSTRALIAN JOURNAL OF GENERAL PRACTICE
(2020)
Review
Pediatrics
Maria Wong, Nicole Williams, Celia Cooper
PEDIATRIC HEALTH MEDICINE AND THERAPEUTICS
(2020)
Review
Health Care Sciences & Services
Brett Ritchie, Kylie Porritt, Tania Marin, Nicole Williams
JBI EVIDENCE SYNTHESIS
(2020)