期刊
MICROBIAL BIOTECHNOLOGY
卷 6, 期 2, 页码 202-206出版社
WILEY-BLACKWELL
DOI: 10.1111/1751-7915.12005
关键词
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资金
- Bundesministerium fur Bildung und Forschung (BMBF) [0313917C]
Carbohydrate oxidases are biotechnologically interesting enzymes that require a tightly or covalently bound cofactor for activity. Using the industrial workhorse Corynebacterium glutamicum as the expression host, successful secretion of a normally cytosolic FAD cofactor-containing sorbitolxylitol oxidase from Streptomyces coelicolor was achieved by using the twin-arginine translocation (Tat) protein export machinery for protein translocation across the cytoplasmic membrane. Our results demonstrate for the first time that, also for cofactor-containing proteins, a secretory production strategy is a feasible and promising alternative to conventional intracellular expression strategies.
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