Article
Multidisciplinary Sciences
Hilde Harb Buzza, Fernanda Alves, Ana Julia Barbosa Tome, Juan Chen, Giulia Kassab, Jiachuan Bu, Vanderlei Salvador Bagnato, Gang Zheng, Cristina Kurachi
Summary: NewPS-aPDT is a safe and effective antimicrobial photosensitizer against biofilms. In experiments, NewPS showed high bactericidal efficacy in suspensions of Streptococcus pneumoniae and Staphylococcus aureus, and enhanced antibacterial effect in S. aureus biofilms with a higher concentration of 100 mu M, longer incubation time, and higher light dose. In a murine-infected wound model, NewPS-aPDT demonstrated better therapeutic efficacy and faster tissue healing compared to other treatment groups.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Microbiology
Yin Li, Ming Ni
Summary: This article discusses the mechanisms of biofilm formation in Klebsiella pneumoniae and its impact on infection treatment. The biofilm produced by K. pneumoniae can shield bacteria from antibiotics and host immune responses, making eradication of the infection more difficult.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Irfan A. Rather, Mohammad Younus Wani, Majid Rasool Kamli, Jamal S. M. Sabir, Khalid Rehman Hakeem, Ahmad Firoz, Yong-Ha Park, Yan-Yan Hor
Summary: This study demonstrates the anti-biofilm and anti-virulence properties of Lactiplantibacillus plantarum KAU007 against Streptococcus pyogenes. The results show that Lactiplantibacillus plantarum KAU007 inhibits the growth and survival of S. pyogenes, as well as the formation of biofilm and the production of virulence-associated factors. This research provides a basis for further investigation into the use of Lactiplantibacillus plantarum KAU007 as an antibacterial agent to combat streptococcal infections.
Review
Immunology
Bijina J. Mathew, Priyal Gupta, Tabassum Naaz, Rupal Rai, Sudheer Gupta, Sudipti Gupta, Shivendra K. Chaurasiya, Shashank Purwar, Debasis Biswas, Ashish Kumar Vyas, Anirudh K. Singh
Summary: Streptococcus pneumoniae (pneumococcus) asymptomatically colonizes the human upper airway but can cause various diseases upon reaching other sites in the body. The bacterium has evolved mechanisms to evade the host's immune attack, including the polysaccharide capsule and surface-associated glycosidases, which help with bacterial survival and adherence. These glycosidases can hydrolyze host glycans and contribute to biofilm formation, making the bacteria resilient to antimicrobials and host immune response.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Infectious Diseases
Ana Gual-de-Torrella, Mercedes Delgado-Valverde, Patricia Perez-Palacios, Jesus Oteo-Iglesias, Estrella Rojo-Molinero, Maria Dolores Macia, Antonio Oliver, Alvaro Pascual, Felipe Fernandez-Cuenca
Summary: This study investigated the role of mrkA adhesin expression, biofilm production, biofilm viability, and biocides in carbapenemase-producing Klebsiella pneumoniae isolates. The results showed that biofilm formation and viability were affected by temperature, with higher rates at 25 degrees Celsius compared to 37 degrees Celsius. Humidity had little effect on biofilm formation. HR clones were more likely to be biofilm producers compared to non-HR clones. Povidone-iodine was found to be effective in reducing biofilm production and thickness.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Microbiology
Chiau-Jing Jung, Chih-Chieh Hsu, Jeng-Wei Chen, Hung-Wei Cheng, Chang-Tsu Yuan, Yu-Min Kuo, Ron-Bin Hsu, Jean-San Chia
Summary: The LiaR-regulated PCP protein in Streptococcus mutans plays a crucial role in bacterial biofilm formation and platelet adhesion. It is well-preserved among pathogenic streptococci and controls biofilm formation in vivo, suggesting its importance in colonization for S. mutans and possibly other pathogenic streptococci.
Article
Microbiology
So-Young Ham, Han-Shin Kim, Eunji Cha, Taehyeung Lim, Youngjoo Byun, Hee-Deung Park
Summary: Raffinose, a natural oligosaccharide, strongly inhibits biofilm formation and GTF-related gene expression in Streptococcus mutans, making it a potential antibiofilm agent for oral supplies and dental materials to prevent dental caries.
MICROBIOLOGY SPECTRUM
(2022)
Article
Immunology
Hannah N. Agnew, John M. Atack, Ann R. D. Fernando, Sophie N. Waters, Mark van der Linden, Erin Smith, Andrew D. Abell, Erin B. Brazel, James C. Paton, Claudia Trappetti
Summary: Streptococcus pneumoniae can switch their genomic DNA methylation pattern between six bacterial subpopulations, which affects phenotype and disease development. The spnIIIB allele is associated with increased nasopharyngeal carriage and downregulation of the luxS gene. In this study, two clinical isolates from a pediatric meningitis patient were analyzed, showing different virulence profiles in mice. Analysis of the spnIII system in strains recovered from infected mice showed switch to different alleles consistent with isolate source, with the blood strain demonstrating high expression of spnIIIB allele. Deletion of luxS gene resulted in phenotypic profiles similar to strains recovered from nasopharynx. This study highlights the important role of the regulatory network between luxS and the type 1 restriction-modification system in S. pneumoniae infections and adaptation to specific host niches.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Hongsheng Ji, Yingshun Zhou, Luhua Zhang, Ying Wang, Feiyang Zhang, Jiawei Bai, Qin Li, Zhikun Zhang
Summary: In this study, the functions of LytA, LytC, and CbpD in biofilm formation were analyzed. It was found that CBD-C and CBD-D promote biofilm formation in Streptococcus pneumoniae. CBD-C promotes the formation of membrane-like structures, while CBD-D promotes the formation of regular reticular structures. In addition, the binding of CBDs affects membrane transport pathways and the bacterial reactions to extracellular substances.
MICROBIAL PATHOGENESIS
(2023)
Article
Agriculture, Multidisciplinary
Ji-Eun Lee, Hee Yeon Cho, Jae Hoon Lee, Dong Uk Ahn, Kee-Tae Kim, Hyun-Dong Paik
Summary: This study investigated the inhibitory effects of ovomucoid, a protein found in egg white, on the biofilm formation of Streptococcus mutans, the primary pathogen associated with tooth decay. The results showed that ovomucoid inhibited biofilm formation, reduced bacterial adhesion and aggregation, and lowered the metabolic activity of bacteria in the biofilm. In addition, ovomucoid decreased the synthesis of water-insoluble EPS by S. mutans. Therefore, ovomucoid has the potential to be used as an anti-biofilm agent for the treatment of dental caries.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Fisheries
Taylor I. Heckman, Esteban Soto
Summary: The globally distributed bacterium Streptococcus iniae is responsible for outbreaks of disease in economically important freshwater and marine fish species, but our understanding of its transmission and infection dynamics remains incomplete. This study found that S. iniae has the ability to form resilient biofilms, providing an effective mechanism for its persistence in the environment. Further research on controlling this pathogen is necessary.
Article
Immunology
Yu-Tze Horng, Novaria Sari Dewi Panjaitan, Hui-Ju Chang, Yu-Hong Wei, Chih-Ching Chien, Hung-Chi Yang, Heng-Yuan Chang, Po-Chi Soo
Summary: In this study, the conservation and role of the KP1_4563 homolog, orfX, in Klebsiella pneumoniae biofilm formation were investigated. The results suggest that orfX can inhibit biofilm formation by reducing capsule polysaccharide (CPS) production.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2022)
Article
Microbiology
Brenda S. S. Antezana, Sarah Lohsen, Xueqing Wu, Jorge E. E. Vidal, Yih-Ling Tzeng, David S. S. Stephens
Summary: Multidrug resistance in Streptococcus pneumoniae remains a global challenge. Tn916-related integrative and conjugative elements (ICEs) are responsible for the dissemination of antibiotic resistance in S. pneumoniae. This study found that Tn2009 can be efficiently transferred between S. pneumoniae strains via transformation and homologous recombination in human nasopharyngeal biofilms.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Peng Liu, Xiaoxia Qin, Tingting Cao, Yuying Yang, Xiaomin Shi, Dejun Liu, Yang Wang, Zhangqi Shen, Shaolin Wang
Summary: This study investigated the potential resistance mechanism of telithromycin resistance in Campylobacter and found that the widely spread 23S_A2075G and erm(B) genes mediate high levels of resistance to telithromycin in C. coli.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Microbiology
Wenhua Huang, Ying Chen, Qian Li, Hua Jiang, Qingyu Lv, Yuling Zheng, Xuelian Han, Decong Kong, Peng Liu, Yongqiang Jiang
Summary: The study revealed that the LytR protein influences the cell morphology, biofilm formation, and virulence of Streptococcus suis (S. suis), playing crucial roles during infection.
VETERINARY MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Lucia Blasco, Anton Ambroa, Rocio Trastoy, Ines Bleriot, Miriam Moscoso, Laura Fernandez-Garcia, Elena Perez-Nadales, Felipe Fernandez-Cuenca, Julian Torre-Cisneros, Jesus Oteo-Iglesias, Antonio Oliver, Rafael Canton, Tim Kidd, Ferran Navarro, Elisenda Miro, Alvaro Pascual, German Bou, Luis Martinez-Martinez, Maria Tomas
SCIENTIFIC REPORTS
(2020)
Article
Immunology
Sara de Miguel, Mirian Domenech, Fernando Gonzalez-Camacho, Julio Sempere, Dolores Vicioso, Juan Carlos Sanz, Luis Garcia Comas, Carmen Ardanuy, Asuncion Fenoll, Jose Yuste
Summary: The study analyzed the invasive pneumococcal disease cases in children and adults from 2009 to 2019, as well as the impact of different vaccine strategies. It found that PCV13 reduced disease burden in both children and adults, but an increase in serotype 8 in adults was observed.
CLINICAL INFECTIOUS DISEASES
(2021)
Review
Immunology
Julio Sempere, Mirella Llamosi, Idoia del Rio Menendez, Beatriz Lopez Ruiz, Mirian Domenech, Fernando Gonzalez-Camacho
Summary: Current vaccines for Streptococcus pneumoniae infections only cover some serotypes, there is a need for new vaccines to cover a wider range of serotypes. Choline-binding proteins are considered potential vaccine candidates as they may protect against different serotypes.
Article
Immunology
Bruno Corsini, Leire Aguinagalde, Susana Ruiz, Mirian Domenech, Jose Yuste
Summary: The study demonstrates that the proteins LytA, LytC, and Pce of Streptococcus pneumoniae can be potential protein antigens for a future universal vaccine, inducing high titers of immunoglobulins and enhancing immunity against pneumococcal strains.
Article
Infectious Diseases
Julio Sempere, Fernando Gonzalez-Camacho, Mirian Domenech, Mirella Llamosi, Idoia Del Rio, Beatriz Lopez-Ruiz, Mercedes Gimeno, Pilar Coronel, Jose Yuste
Summary: This study evaluated the susceptibility of clinical isolates of Streptococcus pneumoniae to a panel of antibiotics from 2004 to 2020. The results showed that cefditoren had the highest proportion of susceptible strains, with over 80% of isolates being susceptible throughout the study period. The rates of resistance to cefditoren and cefotaxime were low, despite the increase in serotypes not covered by the 13-valent pneumococcal conjugate vaccine.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Immunology
Elena Mata, Raquel Tarancon, Claudia Guerrero, Eduardo Moreo, Flavie Moreau, Santiago Uranga, Ana Belen Gomez, Dessislava Marinova, Miriam Domenech, Fernando Gonzalez-Camacho, Marta Monzon, Juan Badiola, Jorge Dominguez-Andres, Jose Yuste, Alberto Anel, Antonio Peixoto, Carlos Martin, Nacho Aguilo
Summary: In this study, pulmonary delivery of BCG was shown to preactivate alveolar macrophages, leading to efficient containment of Mtb and potential protection against respiratory pathogens. The results suggest the generation of a trained innate memory-like response in alveolar macrophages induced by pulmonary BCG vaccination, even after vaccine clearance. Additionally, BCG-induced innate activation also provided heterologous protection against Streptococcus pneumoniae challenge.
SCIENCE IMMUNOLOGY
(2021)
Article
Chemistry, Analytical
Ciro Rodriguez Diaz, Nuria Lafuente-Gomez, Catarina Coutinho, Demian Pardo, Hernan Alarcon-Iniesta, Maria Lopez-Valls, Rocio Coloma, Paula Milan-Rois, Mirian Domenech, Melanie Abreu, Rafael Canton, Juan Carlos Galan, Rebeca Bocanegra, Luis A. Campos, Rodolfo Miranda, Milagros Castellanos, Alvaro Somoza
Summary: This article presents a colorimetric sensor based on gold nanoparticles for the detection of SARS-CoV-2 sequences. The sensor is fast, reliable, and easily scalable, with the ability to detect target sequences within 15 minutes. The efficiency and selectivity of detection are evaluated using synthetic samples, infected cells, and patient samples. The system's sensitivity is enhanced through an affordable amplification approach, enabling the detection of high and medium viral loads. The proposed approach is valuable for facilities with limited resources due to its ease of use, stability, and minimal equipment requirements.
Article
Multidisciplinary Sciences
Julio Sempere, Mirella Llamosi, Federico Roman, Dario Lago, Fernando Gonzalez-Camacho, Covadonga Perez-Garcia, Jose Yuste, Mirian Domenech
Summary: The study demonstrates the effectiveness of Cys and NAC in preventing biofilms formed by Staphylococcus aureus and mixed biofilms of Staphylococcus aureus and Streptococcus pneumoniae.
SCIENTIFIC REPORTS
(2022)
Article
Immunology
Miriam Moscoso, Juan A. Vallejo, Maria P. Cabral, Patricia Garcia, Victor Fuentes-Valverde, Eva Gato, Jorge Arca-Suarez, Pablo Aja-Macaya, German Bou
Summary: This article describes a prototype vaccine for preventing Klebsiella pneumoniae infections, which is constructed using a glutamate racemase-deficient mutant. The results show that this mutant can induce a strong immune response and provide cross-protection against systemic infections caused by highly virulent and multidrug-resistant strains of K. pneumoniae, with a high level of safety.
Article
Immunology
Patricia Garcia, Miriam Moscoso, Ctor Fuentes-Valverde, M. Rosario Rodicio, Silvia Herrera-Leon, German Bou
Summary: This study successfully constructed a safe Salmonella vaccine that provides protection against systemic infection and intestinal inflammation and also exhibits cross-reactive immunity against drug-resistant Salmonella strains.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2023)
Article
Immunology
Victor Fuentes-Valverde, Patricia Garcia, Miriam Moscoso, German Bou
Summary: Pseudomonas aeruginosa is an opportunistic nosocomial pathogen that causes serious respiratory infections, including bacteremia, in immunocompromised or critically ill patients. The emergence of multidrug-resistant strains has complicated treatment. Therefore, the development of prophylactic vaccines is a priority to protect at-risk patients. The inclusion of an additional auxotrophy for D-alanine improves safety and stability, making it a more suitable candidate vaccine against P. aeruginosa respiratory infections.
Article
Immunology
Patricia Garcia, Maria P. Cabral, Alejandro Beceiro, Miriam Moscoso, German Bou
Summary: Staphylococcus aureus is a harmful bacterial pathogen that causes invasive pneumonia in healthcare settings and in the community. The emergence of multidrug resistant strains has limited the treatment options. Developing an effective S. aureus vaccine is a global priority. The vaccine candidate 132 Delta murI Delta dat showed potential in preventing staphylococcal pneumonia, but further research is needed to minimize dose-dependent toxicity for safer intranasal immunization.
Article
Microbiology
Mirella Llamosi, Julio Sempere, Pilar Coronel, Mercedes Gimeno, Jose Yuste, Mirian Domenech
Summary: This study demonstrates the synergistic effect of a third-generation oral cephalosporin (CDN) and an antioxidant (NAC) against pneumococcal biofilms. The combination of CDN and NAC inhibits bacterial growth, destroys biofilms, reduces bacterial adhesion, and effectively clears bacteria from the respiratory tract.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Patricia Garcia, Miriam Moscoso, M. Carmen Fernandez, Victor Fuentes-Valverde, Astrid Perez, German Bou
Summary: This study compared the efficacy of ceftaroline fos-amil with approved therapies for treating experimental MRSA bacteremia. Ceftaroline fosamil was found to be as effective as vancomycin and daptomycin, indicating its potential for treating difficult-to-treat S. aureus bacteremia.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Microbiology
Mirella Llamosi, Julio Sempere, Pilar Coronel, Mercedes Gimeno, Jose Yuste, Mirian Domenech
Summary: Streptococcus pneumoniae is a deadly bacterial pathogen that has caused millions of deaths annually. In this study, a combination of the oral cephalosporin cefditoren and the antioxidant N-acetyl-l-cysteine was found to be synergistic against pneumococcal biofilms. The combination treatment inhibited bacterial growth, destroyed biofilm biomass, reduced bacterial adhesion, and effectively cleared the bacteria from the respiratory tract in a mouse model of acute pneumococcal pneumonia caused by a multidrug-resistant strain.
MICROBIOLOGY SPECTRUM
(2022)