Article
Hematology
Antonina Kurtova, Melanie Heinlein, Simon Haas, Lars Velten, Gerrit J. P. Dijkgraaf, Elaine E. Storm, Noelyn M. Kljavin, Soufiane Boumahdi, Patricia Himmels, Aurelie Herault, Andrew Mancini, Hartmut Koeppen, Monique Dail, Qingxiang Yan, Jianhuan Zhang, Ute Koch, Freddy Radtke, Zora Modrusan, Ciara Metcalfe, Robert Piskol, Frederic J. de Sauvage
Summary: The self-renewal and differentiation of stem and progenitor cells are regulated by systemic and local cues. RSPO3 is important for Wnt signaling and is usually expressed in the stem cell niche to regulate stem cell expansion and differentiation. Disrupting the niche-restricted expression of RSPO3 can affect tissue organization and lead to disorders and cancers.
Article
Chemistry, Medicinal
Jie Cui, Yukimatsu Toh, Soohyun Park, Wangsheng Yu, Jianghua Tu, Ling Wu, Li Li, Joan Jacob, Sheng Pan, Kendra S. Carmon, Qingyun J. Liu
Summary: LGR4-6 are related receptors commonly upregulated in gastrointestinal cancers, with LGR5 being enriched in cancer stem cells. Targeting all three LGRs simultaneously is proposed for a more effective anti-tumor approach. A drug conjugate comprising an RSPO4 mutant and IgG1-Fc showed potent cytotoxic effects on cancer cells expressing any LGR, suppressing tumor growth without adverse effects.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Eline J. ter Steege, Elvira R. M. Bakker
Summary: RSPO proteins play a crucial role in activating the Wnt/beta-catenin signaling pathway in stem cells and progenitor cells. Deregulation of RSPOs has been implicated in various cancer types, with potential for therapeutic targeting in cancer cases with RSPO overrepresentation.
Article
Agriculture, Multidisciplinary
Jia-yi Zhou, Geng-xiu Zan, Qiu-jie Zhu, Chun-qi Gao, Hui-chao Yan, Xiu-qi Wang
Summary: This study cloned the porcine RSPO1 gene and obtained rpRSPO1 protein, and demonstrated the stimulatory effect of rpRSPO1 on jejunal epithelial cell proliferation using in vitro and in vivo models. It also showed that rpRSPO1 could ameliorate growth retardation and maintain epithelial integrity in mice, and increase ISC activity by activating the Wnt/β-catenin pathway.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Hyeyoon Lee, Rui Sun, Christof Niehrs
Summary: Bone morphogenetic proteins (BMPs) play important roles in various biological processes, including embryogenesis and disease. Recent studies have found that R-spondin 2 (RSPO2) acts as a BMP receptor antagonist, and a peptide derived from RSPO2 can uncouple BMP antagonist function from WNT agonist function, showing potential for therapeutic development in diseases like acute myeloid leukemia (AML). By using a dendrimer based on this peptide, it is possible to selectively target BMP receptor signaling, reduce cell growth, and induce differentiation in AML cells, as well as modulate embryonic patterning in Xenopus embryos.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Editorial Material
Biochemistry & Molecular Biology
Ermanno Malagola, Yoku Hayakawa, Timothy C. Wang
Summary: Recent research has identified distinct roles for Lgr4 and Lgr5 in directing gland reconstitution following H. pylori infection, shedding new light on the complexities of Rspo signaling during gastric regeneration and raising questions about antral stem cell hierarchy.
Review
Cell Biology
Joep Beumer, Hans Clevers
Summary: Intestinal stem cells fuel tissue renewal, with the epithelium absorbing nutrients while acting as a barrier, lineage plasticity and niche signals enable efficient tissue repair, new insights from single-cell transcriptomics and organoid models reveal the importance of cell lineage plasticity and niche signals for tissue repair.
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Cecily Lucas, Kay-Sara Sauter, Michael Steigert, Delphine Mallet, James Wilmouth, Julie Olabe, Ingrid Plotton, Yves Morel, Daniel Aeberli, Franca Wagner, Hans Clevers, Amit V. Pandey, Pierre Val, Florence Roucher-Boulez, Christa E. Flueck
Summary: This article describes a highly consanguineous family with multiple perinatal deaths and infants presenting at birth with failure to thrive, severe salt-wasting crises associated with isolated hypoaldosteronism, nail anomalies, short stature, and deafness. Whole exome sequencing revealed a homozygous splice variant in the R-SPONDIN receptor LGR4 gene (c.618-1G>C) regulating WNT signaling. The impact of LGR4 inactivation was analyzed by adrenal cortex-specific ablation of Lgr4, which resulted in abnormal zG differentiation and endocrine function.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Biochemistry & Molecular Biology
Ahmed A. Raslan, Youn Jeong Oh, Yong Ri Jin, Jeong Kyo Yoon
Summary: The lungs have the ability to regenerate damaged tissues caused by acute injury, but many lung diseases are associated with reduced regeneration potential. This study investigates the role of R-spondin2 (RSPO2) in adult lung homeostasis and regeneration. The findings suggest that RSPO2 plays a key role in promoting the regeneration of lung epithelial stem/progenitor cells, and could be a potential therapeutic target for chronic lung diseases with reduced regenerative capability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Joep Sprangers, Irene C. Zaalberg, Madelon M. Maurice
Summary: This review discusses the regenerative response of intestinal epithelial cells to injury, as well as how the Wnt and YAP signaling pathways control intestinal repair. It also emphasizes how organoid-based applications have contributed to unraveling the key mechanisms behind these developmental and regenerative events.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Agriculture, Multidisciplinary
Duoduo Zhang, Xingtao Zhou, Linyuan Liu, Mi Guo, Tongwen Huang, Wengan Zhou, Fang Geng, Steve W. Cui, Shaoping Nie
Summary: The study revealed that glucomannan from Aloe vera gel promoted proliferation and differentiation of intestinal stem cells through the Wnt/β-catenin signaling pathway, facilitating epithelial cell regeneration and alleviating colitis in mice.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Yijun Chen, Zhujin Lu, Jiaxin Feng, Zefeng Chen, Zejian Liu, Xiuqi Wang, Huichao Yan, Chunqi Gao
Summary: In this study, it was found that treatment with rRSPO1 can activate hair follicle stem cells in mice and accelerate hair regeneration. rRSPO1 improves hair follicle development and promotes hair growth by activating the Wnt/β-catenin signaling pathway.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2023)
Article
Cardiac & Cardiovascular Systems
Chong Chen, Hang Qu, Fang Liu, Yu Yu, Kun Sun, Alex F. Chen
Summary: This article investigates the regulatory role of R-spondin 3 in high glucose-induced endothelial activation. It demonstrates that R-spondin 3 can inhibit endothelial activation and reveals the underlying mechanism involving the leucine-rich G protein-coupled receptor 4/Wnt/β-catenin pathway.
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
(2022)
Review
Oncology
Esther Martinez-Font, Marina Perez-Capo, Oliver Vogler, Javier Martin-Broto, Regina Alemany, Antonia Obrador-Hevia
Summary: Soft tissue sarcomas are a heterogeneous group of rare tumors originating from mesenchymal tissue, with chemotherapy based on doxorubicin remaining the standard first-line treatment. Understanding the molecular alterations of the WNT signaling pathway in STS is crucial for developing new therapeutic approaches and identifying drug targets.
Article
Cell Biology
Lubna M. Mehdawi, Souvik Ghatak, Payel Chakraborty, Anita Sjolander, Tommy Andersson
Summary: This study finds that WNT5A signaling inhibits colon cancer stemness and VEGFA expression by suppressing the expression of LGR5/RSPO3 and beta-catenin activity. This provides clues for developing new strategies to treat colon cancer.
Article
Biochemistry & Molecular Biology
B. A. Wilky, C. Kim, G. McCarty, E. A. Montgomery, K. Kammers, L. R. DeVine, R. N. Cole, V. Raman, D. M. Loeb
Article
Oncology
Kim A. Reiss, Deepa Rangachari, David Cosgrove, Breelyn Wilky, Ross Donehower
JOURNAL OF CANCER EDUCATION
(2017)
Review
Oncology
Vaia Florou, Breelyn A. Wilky, Jonathan C. Trent
Review
Oncology
Vaia Florou, Breelyn A. Wilky
CURRENT TREATMENT OPTIONS IN ONCOLOGY
(2018)
Review
Oncology
Ana Sebio, Breelyn A. Wilky, Vicki L. Keedy, Robin L. Jones
Article
Oncology
Santhosh A. Upadhyaya, Rose B. McGee, Breelyn A. Wilky, Alberto Broniscer
PEDIATRIC BLOOD & CANCER
(2018)
Review
Orthopedics
Nisreen S. Ezuddin, Juan Pretell-Mazzini, Raphael L. Yechieli, Darcy A. Kerr, Breelyn A. Wilky, Ty K. Subhawong
SKELETAL RADIOLOGY
(2018)
Article
Oncology
Stephen J. Ramey, Raphael Yechieli, Wei Zhao, Joyson Kodiyan, David Asher, Felix M. Chinea, Vivek Patel, Isildinha M. Reis, Lily Wang, Breelyn A. Wilky, Ty Subhawong, Jonathan C. Trent
Article
Oncology
Francis Hall, Victor Villalobos, Breelyn Wilky
CURRENT PROBLEMS IN CANCER
(2019)
Review
Immunology
Breelyn A. Wilky
IMMUNOLOGICAL REVIEWS
(2019)
Article
Oncology
Breelyn A. Wilky, Matte M. Trucco, Ty K. Subhawong, Vaia Florou, Wungki Park, Deukwoo Kwon, Eric D. Wieder, Despina Kolonias, Andrew E. Rosenberg, Darcy A. Kerr, E. Rosyni Sfakianaki, Mark Foley, Jaime R. Merchan, Krishna Komanduri, Jonathan C. Trent
Review
Surgery
Kristina Khazeni, Hannah LaBove, Breelyn Wilky, Andrew E. Rosenberg, Elizabeth Paulus, Harvey Chim, Joseph M. Pearson, Danny Yakoub
INTERNATIONAL JOURNAL OF SURGERY CASE REPORTS
(2018)
Article
Oncology
Andrea P. Espejo, Jeremy L. Ramdial, Breelyn A. Wilky, Darcy A. Kerr, Jonathan C. Trent
Article
Medicine, Research & Experimental
Breelyn A. Wilky, John M. Goldberg
DISCOVERY MEDICINE
(2017)
Review
Biotechnology & Applied Microbiology
Y. Tan, J. C. Trent, B. A. Wilky, D. A. Kerr, A. E. Rosenberg
CANCER GENE THERAPY
(2017)