Article
Polymer Science
Lixia Huang, Yifei Jiang, Xinying Chen, Wenqi Zhang, Qiuchen Luo, Siyan Chen, Shuhan Wang, Fangqing Weng, Lin Xiao
Summary: Supramolecular responsive microcarriers based on chitosan microspheres were developed for nonenzymatic cell detachment. These microcarriers showed excellent biocompatibility and gelatin niches, allowing for effective cell attachment and expansion. Cells could be detached from the microcarriers through competitive binding.
Article
Biotechnology & Applied Microbiology
Shenyang Li, Chengcheng Ding, Yonglong Guo, Yanan Zhang, Hao Wang, Xihao Sun, Jun Zhang, Zekai Cui, Jiansu Chen
Summary: The biophysical properties of PDMS promote reprogramming of corneal keratocytes into stem-like cells, as seen in the higher expression of stem cell marker genes and endogenous stemness transcription factors compared to corneal fibroblasts. Additionally, PDMS regulates various signaling pathways and mechanisms related to mechanotransduction, cellular transition, and hypoxia, highlighting its potential in facilitating cellular reprogramming.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Environmental
Xiaolei Chen, Dong Zhang, Xinqing Wang, Zhenxi Liu, Huili Kang, Changsheng Liu, Fangping Chen
Summary: In this study, a low-cost, easy-assembly, and scalable method for preparing GelMA PMS for bone marrow mesenchymal stem cells (BMSCs) expansion is proposed. The pore size of GelMA PMS can be controlled by adjusting the freezing temperature. GelMA PMS exhibited higher cell attachment ratio, cell spreading area, and differentiation potential compared to commercial microcarriers.
CHEMICAL ENGINEERING JOURNAL
(2023)
Review
Pharmacology & Pharmacy
Lisa Belfiore, Behnaz Aghaei, Andrew M. K. Law, Jeremy C. Dobrowolski, Lyndon J. Raftery, Angie D. Tjandra, Christine Yee, Alberto Piloni, Alexander Volkerling, Cameron J. Ferris, Martin Engel
Summary: 3D cell models are considered more accurate in predicting the efficacy of drug responses and are seen as more physiologically representative tools for in vitro modeling of in vivo tissues. In the future, 3D cell models will continue to play an important role in drug discovery applications.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Review
Biotechnology & Applied Microbiology
Chunyan Duan, Mingjia Yu, Changji Hu, Hongying Xia, Ranjith Kumar Kankala
Summary: Tissue engineering aims to restore tissue defects using 3D biomimetic pre-formed scaffolds. Micro-sized injectable modularized units have gained interest as ideal delivery vehicles for cells and growth factors due to their minimally-invasive nature and improved cell retention efficacy. This review discusses the progress of these carriers in tissue engineering, highlighting their design and ability to deliver cells and promote tissue growth.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Genetics & Heredity
Anna Barbara Di Stefano, Federica Grisafi, Mileidys Perez-Alea, Marta Castiglia, Marta Di Simone, Serena Meraviglia, Adriana Cordova, Francesco Moschella, Francesca Toia
Summary: Adipose stem cells have great potential in regenerative medicine, with 3D models potentially mimicking native tissue, and SASCs-3D showing enhanced regenerative abilities. Gene expression varies significantly between 2D and 3D cultures, especially regarding stemness, aging, telomere maintenance, and oxidative stress. More investigation is needed on the implications of these differences for cell quality.
Article
Chemistry, Multidisciplinary
Seyed Mohammad Hossein Dabiri, Ehsan Samiei, Shahla Shojaei, Lucas Karperien, Bardia Khun Jush, Tavia Walsh, Maryam Jahanshahi, Sadegh Hassanpour, David Hamdi, Amir Seyfoori, Samad Ahadian, Ali Khademhosseini, Mohsen Akbari
Summary: A multifunctional temperature-responsive microcarrier was developed in this study for cell culture and growth, with properties such as temperature regulation and enhanced stiffness, providing good support for the growth of human-derived glioma cells.
Article
Chemistry, Analytical
Huijung Kim, Yoojoong Han, Intan Rosalina Suhito, Yoon Choi, Minkyeong Kwon, Hyungbin Son, Hyung-Ryong Kim, Tae-Hyung Kim
Summary: This study utilized a 3D Raman mapping-based analytical method to investigate the 3D cellular differentiation of human dental pulp stem cell spheroids under different culture conditions. It was found that the diffusion of the medium played a crucial role in determining the variations in stem cell spheroid differentiation.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Nina Compera, Scott Atwell, Johannes Wirth, Christine von Toerne, Stefanie M. Hauck, Matthias Meier
Summary: This study developed a microfluidic chip platform to simulate adipose tissue in vitro and revealed the differences in adipocytes under low/high glucose conditions using proteomics analysis. This in vitro model can be used to investigate the molecular and functional mechanisms of adipose tissue under normal and pathological conditions.
Article
Engineering, Biomedical
Xihao Sun, Zekai Cui, Yuqin Liang, Chunwen Duan, Hon Fai Chan, Shengru Mao, Jianing Gu, Chengcheng Ding, Xu Yang, Qing Wang, Shibo Tang, Jiansu Chen
Summary: We have successfully differentiated three-dimensional retinal organoids (ROs) with growth and development similar to the human retina from human induced pluripotent stem cells (hiPSCs) using a novel polydimethylsiloxane (PDMS) microwell platform. Our results showed that the ROs could uniformly adhere and differentiate into mature cells, maintaining homogeneity over a long period of time. We also established a xeno-free culture workflow by replacing fetal bovine serum with human platelet lysate, facilitating clinical application.
Review
Engineering, Biomedical
Changmin Shao, Junjie Chi, Luoran Shang, Qihui Fan, Fangfu Ye
Summary: Droplet microfluidic technology is a new platform for controlling the generation of microdroplets and droplet-derived materials. It has unique advantages in the preparation of functional microcarriers and is widely used in the areas of cell culture, tissue engineering, and drug delivery.
ACTA BIOMATERIALIA
(2022)
Article
Chemistry, Physical
Lingjiao Zou, Weiqing Zhou, Xiangming Na, Naoki Asakawa, Hua Yue, Guanghui Ma
Summary: 3D cell carriers with porous structures have the potential to investigate drug treatments for tumors. The fabrication of gigantic highly-porous microcarriers allows for high-efficiency 3D cell culture and the construction of artificial tumor tissue models.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2023)
Review
Biochemistry & Molecular Biology
Zuzanna Bober, David Aebisher, Marcin Olek, Aleksandra Kawczyk-Krupka, Dorota Bartusik-Aebisher
Summary: Magnetic resonance imaging (MRI) is an important diagnostic imaging method that has been widely used in pharmaceutical science for cell culture research. MRI provides both anatomical and pathological information, and with advanced technology, it can now also provide physiological information. Cell cultures play a crucial role in cancer research and stem cell research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Feng Yang, Shouwei Wang, Yingying Li, Shilei Li, Wenting Liu, Yushuang Li, Haijuan Hu
Summary: This study focuses on optimizing parameters for large-scale cell cultures, exploring the potential applications of cell engineering and cell-based meat, and providing references for cell therapy of muscular dystrophy and industrialized production of cell-based meat.
Article
Chemistry, Multidisciplinary
Mona Kappes, Bernhard Friedrich, Felix Pfister, Christian Huber, Ralf Phillipp Friedrich, Rene Stein, Christian Braun, Julia Band, Eveline Schreiber, Christoph Alexiou, Christina Janko
Summary: Noncontact manipulation of cells in regenerative medicine using superparamagnetic iron oxide nanoparticles (SPIONs) allows for new possibilities in tissue engineering. SPIONs can be used as drug transporters, contrast agents, or to make cells maneuverable by magnetic forces. Different SPION coatings, such as PAM and citrate, show good cellular loading and magnetic controllability of cells. This research demonstrates the potential for using SPIONs in 3D cell cultures and highlights their ability to accelerate spheroid formation and micropatterning.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Editorial Material
Biochemical Research Methods
Janay Elise Kong, Jaekyung Koh, Jonathan Lin, Dino Di Carlo
Article
Biochemical Research Methods
Anja Kunze, Coleman Tylor Murray, Chanya Godzich, Jonathan Lin, Keegan Owsley, Andy Tay, Dino Di Carlo
Article
Nanoscience & Nanotechnology
Jonathan Lin, Donghyuk Kim, Henry T. Tse, Peter Tseng, Lillian Peng, Manjima Dhar, Saravanan Karumbayaram, Dino Di Carlo
MICROSYSTEMS & NANOENGINEERING
(2017)
Article
Chemistry, Multidisciplinary
Christine E. Wang, Roma C. Yumul, Jonathan Lin, Yilong Cheng, Andre Lieber, Suzie H. Pun
JOURNAL OF CONTROLLED RELEASE
(2018)
Article
Engineering, Biomedical
Ivan Pushkarsky, Peter Tseng, Dylan Black, Bryan France, Lyndon Warfe, Cynthia J. Koziol-White, William F. Jester, Ryan K. Trinh, Jonathan Lin, Philip O. Scumpia, Sherie L. Morrison, Reynold A. Panettieri, Robert Damoiseaux, Dino Di Carlo
NATURE BIOMEDICAL ENGINEERING
(2018)
Article
Chemistry, Multidisciplinary
Peter Tseng, Jonathan Lin, Keegan Owsley, Janay Kong, Anja Kunze, Coleman Murray, Dino Di Carlo
ADVANCED MATERIALS
(2015)
Article
Chemistry, Analytical
Hector E. Munoz, Ming Li, Carson T. Riche, Nao Nitta, Eric Diebold, Jonathan Lin, Keegan Owsley, Matthew Bahr, Keisuke Goda, Dino Di Carlo
ANALYTICAL CHEMISTRY
(2018)
Editorial Material
Biochemical Research Methods
Chueh-Yu Wu, Oladunni Adeyiga, Jonathan Lin, Dino Di Carlo
Article
Computer Science, Interdisciplinary Applications
Songzhe Xu, Fei Xu, Aditya Kommajosula, Ming-Chen Hsu, Baskar Ganapathysubramanian
COMPUTERS & FLUIDS
(2019)
Article
Mechanics
Aditya Kommajosula, Daniel Stoecklein, Dino Di Carlo, Baskar Ganapathysubramanian
JOURNAL OF FLUID MECHANICS
(2020)
Article
Green & Sustainable Science & Technology
Sophia Ghanimeh, Charbel Abou Khalil, Daniel Stoecklein, Aditya Kommasojula, Baskar Ganapathysubramanian
Article
Biochemical Research Methods
Jeong-ah Kim, Aditya Kommajosula, Yo-han Choi, Je-Ryung Lee, Eun-chae Jeon, Baskar Ganapathysubramanian, Wonhee Lee