Article
Chemistry, Analytical
Minggang Tian, Baoli Dong, Zheming Zhang, Junling Yin, Weiying Lin
Summary: A new fluorescent probe mPTP-F has been successfully designed to monitor the opening of mPTP in cellular native status. This probe can serve as an important tool for studying areas such as ischemia-reperfusion injury and cell apoptosis.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Francisco J. Sanchez-Rivera, Jeremy Ryan, Yadira M. Soto-Feliciano, Mary Clare Beytagh, Lucius Xuan, David M. Feldser, Michael T. Hemann, Jesse Zamudio, Nadya Dimitrova, Anthony Letai, Tyler Jacks
Summary: The level of mitochondrial apoptotic priming is a critical determinant of cell fate upon p53 reactivation, with cells having high initial priming tending to undergo apoptosis and cells with low priming tending to survive and arrest in the cell cycle. Manipulating the priming levels through BCL-2 or BCL-XL expression or inhibition can affect the outcome of p53 restoration, highlighting mitochondrial apoptotic priming as a key factor in determining cell fate. Moreover, less primed cells can be forced into apoptotic cell fate following p53 activation using p53-independent drugs that increase apoptotic priming.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Hudie Wei, Lingzhi Qu, Shuyan Dai, Yun Li, Haolan Wang, Yilu Feng, Xiaojuan Chen, Longying Jiang, Ming Guo, Jun Li, Zhuchu Chen, Lin Chen, Ye Zhang, Yongheng Chen
Summary: The structure of p53/BCL-xL complex reveals the molecular basis of their interaction and provides insight into the mechanisms of p53-mediated mitochondrial apoptosis.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Emmanouil Zacharioudakis, Bogos Agianian, Vasantha Kumar Mv, Nikolaos Biris, Thomas P. Garner, Inna Rabinovich-Nikitin, Amanda T. Ouchida, Victoria Margulets, Lars Ulrik Nordstrom, Joel S. Riley, Igor Dolgalev, Yun Chen, Andre J. H. Wittig, Ryan Pekson, Chris Mathew, Peter Wei, Aristotelis Tsirigos, Stephen W. G. Tait, Lorrie A. Kirshenbaum, Richard N. Kitsis, Evripidis Gavathiotis
Summary: Mitofusins are proteins that regulate mitochondrial fusion, a process with significant impact on cellular processes. This study identifies small molecules that can activate or inhibit Mitofusins' activity, thereby modulating mitochondrial fusion and functionality. Inhibition of mitochondrial fusion leads to certain physiological changes such as caspase-3/7 activation and DNA damage. The findings highlight the importance of Mitofusins' conformational changes and oligomerization in enabling mitochondrial fusion. The study provides insights into the function and regulation of Mitofusins and offers potential small molecules for pharmacological targeting.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Sisi Wu, Jie Lan, Lingyu Li, Xiaoxiao Wang, Mingming Tong, Li Fu, Yanjing Zhang, Jiayi Xu, Xuemei Chen, Hongying Chen, Ruli Li, Yao Wu, Juanjuan Xin, Xin Yan, He Li, Kunyue Xue, Xue Li, Caili Zhuo, Wei Jiang
Summary: Sirt6 plays a crucial role in mitigating oxidative stress and cardiac injury by upregulating endogenous antioxidants and suppressing ROS-stirred p53-dependent oxidative stress and cell death signaling pathways, thereby protecting the heart against DOX-induced cardiotoxicity.
CELL BIOLOGY AND TOXICOLOGY
(2023)
Article
Anatomy & Morphology
Hao Wan, Yan-di Yan, Xi-min Hu, Lei Shang, Yu-hua Chen, Yan-xia Huang, Qi Zhang, Wei-tao Yan, Kun Xiong
Summary: Ischemia-reperfusion (I/R) injury is a common pathological mechanism in retinal diseases, and mitochondrial dysfunction plays a critical role in cell death. Voltage-dependent anion channel 1 (VDAC1) regulates the permeability of mitochondrial membrane by controlling its oligomerization. This study demonstrates that inhibition of VDAC1 oligomerization can rescue mitochondrial dysfunction and reduce cell death in retinal I/R injury, suggesting a potential therapeutic strategy for ocular diseases caused by I/R injury.
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
(2023)
Review
Cell Biology
Qian Hao, Jiaxiang Chen, Hua Lu, Xiang Zhou
Summary: The tumor-suppressive activity of p53 is mainly due to its ability to induce cell death, specifically apoptosis, through various mechanisms. It transcriptionally activates pro-apoptotic genes and inhibits anti-apoptotic genes, leading to mitochondrial apoptosis. It also promotes mitochondrial apoptosis by directly interacting with BCL-2 family proteins in the mitochondria. ARTS, a pro-apoptotic protein encoded by an alternative spliced variant of the SEPT4 gene, facilitates BCL-2 and XIAP degradation, triggering apoptosis. ARTS is a new p53 target gene that enhances mitochondrial apoptosis by interacting with p53. This review discusses the mechanisms of p53-induced mitochondrial apoptosis, the role of ARTS in regulating mitochondrial cell death, and the clinical significance of ARTS in cancer and non-cancer diseases.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2023)
Review
Oncology
Massimo Bonora, Sonia Missiroli, Mariasole Perrone, Francesco Fiorica, Paolo Pinton, Carlotta Giorgi
Summary: Cancer cells display genomic instability, with mitochondria playing a key role in responding to DNA alterations and affecting cell survival and the accumulation of mutations. Mitochondrial alterations in cancer can impact cell death pathways and metabolic plasticity, contributing to the evolution of the disease. Mitochondria are involved in regulating cell death and metabolic adaptation in tumor cells, and their role in supporting genomic instability in cancer is an important aspect that warrants further exploration.
Article
Environmental Sciences
XiangFei Zhang, YuTing Wang, Ming Chen, Ming Zeng
Summary: Studies have shown that hexavalent chromium can induce p53-independent apoptosis through calcium overload. The activation of the Ca2+/CaM/CaMKII signaling pathway and changes in apoptosis-associated factors play important roles in this process.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Cell Biology
Wenjia Xie, Qingyun He, Yan Zhang, Xinxin Xu, Ping Wen, Hongdi Cao, Yang Zhou, Jing Luo, Junwei Yang, Lei Jiang
Summary: Mitochondrial fragmentation is an important pathological process in acute kidney injury (AKI), leading to cell death. This study found that phosphorylation of PKM2 contributed to its dimer formation and translocation into mitochondria. Mitochondrial PKM2 binds MYH9 to promote mitochondrial fragmentation. Modulating PKM2 abundance and activity to inhibit mitochondrial translocation may alleviate renal tubular injury and cell death.
CELL DEATH & DISEASE
(2023)
Review
Biochemistry & Molecular Biology
Angeles Almeida, Irene Sanchez-Moran, Cristina Rodriguez
Summary: Stroke is a major cause of death and disability in adults, with neuronal apoptosis playing a key role in ischemic brain damage and impaired recovery. The tumor suppressor protein p53 regulates cellular processes and its subcellular localization, especially in mitochondria, significantly affects neuronal apoptosis and survival after stroke.
Article
Agriculture, Multidisciplinary
Keer Ma, Zhigao Wang, Xingrong Ju, Jiankang Huang, Rong He
Summary: In this study, it was found that rapeseed peptide can inhibit the proliferation of HepG2 cells and induce cell apoptosis through the mitochondrial pathway. The rapeseed peptide activates the P53 signaling pathway and inhibits MDM2, a negative regulator of P53, promoting the activation of P53.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Geriatrics & Gerontology
Peizheng Li, Hongbo Lv, Bohan Zhang, Ruonan Duan, Xiufang Zhang, Pengfei Lin, Chengyuan Song, Yiming Liu
Summary: This study found that GDF15 can protect mitochondrial function and reduce apoptosis in PD cell models by upregulating PGC1 alpha. Additionally, GDF15 overexpression can improve cell protection by activating the PI3K/Akt/mTOR signaling pathway.
FRONTIERS IN AGING NEUROSCIENCE
(2022)
Article
Oncology
Joshua H. Choe, Tatsuya Kawase, An Xu, Asja Guzman, Aleksandar Z. Obradovic, Ana Maria Low-Calle, Bita Alaghebandan, Ananya Raghavan, Kaitlin Long, Paul M. Hwang, Joshua D. Schiffman, Yan Zhu, Ruiying Zhao, Dung -Fang Lee, Chen Katz, Carol Prives
Summary: This study investigates the pathogenic mechanism of cancer susceptibility genes and finds that the p53(A347D) mutation leads to the inability of p53 to form tetramers, resulting in a hyperstable dimeric state and the inability to bind and activate most canonical p53 target genes, but possessing neomorphic functions. Cells with the p53(A347D) mutation have similar tumorigenic capabilities compared to cells without p53, but the mutation can induce metabolic reprogramming and severe mitochondrial network abnormalities, and can drive apoptotic cell death via mitochondrial association in the absence of transcriptional activity upon topoisomerase II inhibition.
Article
Chemistry, Medicinal
Jingbo Qin, Boning Niu, Xiaohui Chen, Cheng Hu, Sheng Lu, Hongsheng Li, Weihao Liu, Jiayi Li, Zihao Teng, Yinghuang Yang, Hongyu Hu, Yang Xu, Shuaidong Huo, Zhen Wu, Yingkun Qiu, Hu Zhou, Meijuan Fang
Summary: The orphan nuclear receptor Nur77 has been validated as a potential drug target for treating breast cancer. In this study, the compound ja showed improved binding capability to Nur77 and exhibited excellent antiproliferative activities against breast cancer cell lines, specifically triple-negative breast cancer cells.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Cardiac & Cardiovascular Systems
Peng Lu, Yi-Qian Zhang, Hao Zhang, Yu-Feng Li, Xiao-Yu Wang, Hao Xu, Zhi-Wei Liu, Lei Li, Hong-Yan Dong, Zhong-Ming Zhang
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2016)
Article
Cell Biology
Chenlong Yi, Meijuan Song, Lifu Sun, Linjie Si, Dongmin Yu, Ben Li, Peng Lu, Wei Wang, Xiaowei Wang
Summary: This study investigates the protective effects of asiatic acid (AA) in myocardial ischemia-reperfusion injury (MIRI) and its underlying mechanisms. The results show that AA exerts a protective effect on MIRI by improving cardiac function and reducing cardiomyocyte damage. It does so by alleviating mitochondrial oxidative stress and inhibiting the mitochondria-dependent apoptosis pathway.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Article
Biology
Ben Li, Chufan Wang, Peng Lu, Yumeng Ji, Xufeng Wang, Chaoyang Liu, Xiaohu Lu, Xiaohan Xu, Xiaowei Wang
Summary: In our study, we investigated the role of IDH1 in atherosclerotic foam cells. We found that inhibiting IDH1 can reduce macrophage ferroptosis and foam cell formation, which may provide insights into the occurrence and progression of atherosclerosis and potential treatment targets. The study also revealed the importance of ferroptosis and IDH1 in foam cell production.
Article
Cardiac & Cardiovascular Systems
Chenlong Yi, Fangyu Chen, Rongrong Ma, Zhi Fu, Meijuan Song, Zhuan Zhang, Lingdi Chen, Xing Tang, Peng Lu, Ben Li, Qingfen Zhang, Qifeng Song, Guangzheng Zhu, Wei Wang, Qiang Wang, Xiaowei Wang
Summary: This study demonstrates that elevated serum level of calpains after CPB cardiac surgery is associated with impaired lung function and poor clinical outcomes, suggesting that serum level of calpains could serve as a potential biomarker for postoperative ALI in adults.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Cardiac & Cardiovascular Systems
Peng Lu, Xiaohu Lu, Ben Li, Chufan Wang, Xufeng Wang, Yumeng Ji, Zhaoyang Liu, Xiangyu Li, Chenlong Yi, Meijuan Song, Xiaowei Wang
Summary: This study investigates the relationship between preoperative high-sensitivity cardiac troponin T (hs-cTnT) and early postoperative hypoxemia (EPH) following off-pump coronary artery bypass grafting (OPCAB). The findings suggest that preoperative hs-cTnT levels are associated with EPH and other adverse outcomes. Therefore, evaluating preoperative hs-cTnT concentrations can help identify patients who would benefit from OPCAB.
JOURNAL OF CARDIOVASCULAR DEVELOPMENT AND DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)