Article
Immunology
Haoxin Shi, Dong Li, Qing Shi, Zhenxia Han, Yuwei Tan, Xiaodong Mu, Miao Qin, Zengjun Li
Summary: The study established a 3D culture system for macrophages using collagen microcarriers, finding that macrophages grown in 3D culture had significantly different morphology, arrangement, and angiogenic capabilities compared to those in 2D culture. Whole-transcriptome sequencing revealed significant gene expression differences, including downregulation of important angiogenic factors, and decreased expression of genes related to key angiogenic pathways in 3D-cultured macrophages.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Jinchul Ahn, Kyungeun Ohk, Jihee Won, Dong-Hee Choi, Yong Hun Jung, Ji Hun Yang, Yesl Jun, Jin-A Kim, Seok Chung, Sang-Hoon Lee
Summary: The reconstruction of skin equivalents with physiologically relevant cellular and matrix architecture is crucial for basic research and industrial applications. A three-dimensional innervated epidermal keratinocyte layer as a sensory neuron-epidermal keratinocyte co-culture model has been developed, which can recapitulate a more organized stratification and enhanced barrier function. This model has the potential to be an improved surrogate model and platform for biomedical and pharmaceutical research.
NATURE COMMUNICATIONS
(2023)
Article
Cell & Tissue Engineering
Luca Pontiggia, Ingmar A. J. Van Hengel, Agnes Klar, Dominic Rutsche, Monica Nanni, Andreas Scheidegger, Sandro Figi, Ernst Reichmann, Ueli Moehrlen, Thomas Biedermann
Summary: This study demonstrates the feasibility of 3D bioprinting pigmented and pre-vascularized skin substitutes using autologous skin cells. The successful implementation of this method offers potential for treating burns and severe skin lesions.
JOURNAL OF TISSUE ENGINEERING
(2022)
Article
Oncology
Demetris Papamichael, Guilherme S. Lopes, Curt L. Olswold, Jean-Yves Douillard, Richard A. Adams, Timothy S. Maughan, Eric Van Cutsem, Alan P. Venook, Heinz-Josef Lenz, Volker Heinemann, Richard Kaplan, Carsten Bokemeyer, Benoist Chibaudel, Axel Grothey, Takayuki Yoshino, John Zalcberg, Aimery De Gramont, Qian Shi
Summary: The efficacy and safety of adding anti-EGFR agents to doublet chemotherapy in older patients were investigated. The results showed that adding anti-EGFR agents did not significantly improve progression-free survival and overall survival in older patients. In contrast, younger patients showed better efficacy and survival after the addition of anti-EGFR agents.
EUROPEAN JOURNAL OF CANCER
(2022)
Review
Pharmacology & Pharmacy
Rahul S. Rathore, Senthil Ayyannan, Sanjeev K. Mahto
Summary: This review highlights the application potential of in vitro 3D neuronal cell culture techniques in screening and developing neurodegenerative disorder drugs, and discusses the advantages and challenges associated with these techniques.
EXPERT OPINION ON DRUG DISCOVERY
(2022)
Review
Biotechnology & Applied Microbiology
Christine Yee, Kristie-Ann Dickson, Mohammed N. Muntasir, Yue Ma, Deborah J. Marsh
Summary: Ovarian cancer has the highest mortality rate among gynecological malignancies. Different histotypes of the cancer respond differently to treatments, with chemoresistance being a key factor in incurable disease. While PARP inhibitors and bevacizumab are currently used in clinical settings, further research is needed to predict patient responses to these drugs effectively.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Dermatology
Christianna Marie Czyz, Paul Werner Kunth, Florian Gruber, Christopher Kremslehner, Christoph Matthias Hammers, Jennifer Elisabeth Hundt
Summary: Human skin equivalents (HSEs) are three-dimensional skin organ culture models raised in vitro. This review provides an overview of common techniques for setting up HSEs, including the use of 3T3-J2 murine fibroblasts, purchased human fibroblasts or freshly isolated and cultured fibroblasts to build the scaffold, and freshly isolated and cultured keratinocytes to seed on top. It also discusses the addition of other cell types such as melanocytes and provides possible markers and techniques for evaluating artificial skin. The review further compares HSEs to human skin organ culture and discusses their advantages, limitations, and future perspectives.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Oncology
Joana G. Rodrigues, Henrique O. Duarte, Catarina Gomes, Meritxell Balmana, Alvaro M. Martins, Paul J. Hensbergen, Arnoud H. de Ru, Jorge Lima, Andre Albergaria, Peter A. van Veelen, Manfred Wuhrer, Joana Gomes, Celso A. Reis
Summary: The study analyzed the role of EGFR-specific terminal alpha 2,6-sialylation in modulating the malignant phenotype of CRC cells and their resistance to Cetuximab. The results showed that CRC cells with increased ST6Gal1 expression and displaying terminal alpha 2,6-sialylation exhibited marked resistance to Cetuximab-induced cytotoxicity.
Article
Engineering, Multidisciplinary
Ildi Troka, Gabriele Griffanti, Lucie Canaff, Geoffrey N. Hendy, David Goltzman, Showan N. Nazhat
Summary: Bone extracellular matrix (ECM) is crucial for osteoblast differentiation and matrix mineralization. In this study, a biomimetic 3D osteoid-like collagen gel model was used to investigate the role of menin in osteoblastic differentiation and mineralization. The findings suggest that menin plays an important role in maintaining matrix mineralization in the early stages of osteoblast differentiation.
Article
Engineering, Environmental
Jun-Ray Macairan, Brian Nguyen, Frank Li, Nathalie Tufenkji
Summary: An effective methodology is required to detect unlabeled microplastics in environmental samples in order to understand the biological impacts of plastic pollution. Current methods rely on digestion protocols which can result in the loss of spatial information and smaller plastics. The use of fluorescence microscopy for visualization is limited by nonspecific labeling strategies. A new method of nonspecific labeling with tissue-clearing technique is developed, allowing clear visualization of internalized microplastics. The method is demonstrated in living aquatic organisms and a model vertebrate.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Review
Chemistry, Medicinal
Shaimaa M. Badr-Eldin, Hibah M. Aldawsari, Sabna Kotta, Pran Kishore Deb, Katharigatta N. Venugopala
Summary: Despite the advancements in drug discovery, it remains a tedious and expensive process. 3D cell culture models are considered promising in mimicking in vivo conditions and bridging the gap between 2D cell culture and animal models. However, there are challenges such as maintaining a micro-tissue environment and reproducibility. This review emphasizes different 3D techniques and their potential role in drug repositioning, including perspectives from the COVID-19 pandemic.
Review
Biochemistry & Molecular Biology
Camille Jubelin, Javier Munoz-Garcia, Laurent Griscom, Denis Cochonneau, Emilie Ollivier, Marie-Francoise Heymann, Francois M. Vallette, Lisa Oliver, Dominique Heymann
Summary: Cancer is a complex disease that requires multidimensional models to understand its development and drug response. Three-dimensional cell culture models, compared to traditional two-dimensional cultures, better mimic the biological behavior of tumor cells and provide a better platform for studying therapeutic escape and drug resistance.
CELL AND BIOSCIENCE
(2022)
Article
Reproductive Biology
Shaina L. L. Jachter, Wilson P. P. Simmons, Charles Estill, Jing Xu, Cecily V. Bishop
Summary: Disrupted ovarian steroidogenesis is associated with fertility disorders. In this study, a novel matrix-free 3D culture system was used to culture dairy-breed cow follicles. The addition of epidermal growth factor (EGF) significantly improved follicle survival rates. This system provides an excellent in vitro model to explore processes associated with folliculogenesis in cattle.
Article
Mechanics
Qiang Zhang, Fangyi Li, Dachang Zhu, Hongxin Wang
Summary: This paper presents a new design for a three-dimensional compression-torsion mechanical metamaterial based on the three-period minimal surface (TPMS) theory. This design concept shows superior torsion performance and a negative Poisson's ratio (NPR) effect compared to previous structures. The findings have significant implications for smart devices, aerospace engineering, and conservation engineering applications.
COMPOSITE STRUCTURES
(2023)
Article
Engineering, Manufacturing
I. R. Cozar, F. Otero, P. Maimi, E. Gonzalez, S. Miot, A. Turon, P. P. Camanho
Summary: A new 3D elastoplastic damage model is proposed to predict the plastic deformation and progressive failure of composite materials. The model is validated through various tests and shows good agreement with experimental data.
COMPOSITES PART A-APPLIED SCIENCE AND MANUFACTURING
(2022)
Article
Biochemistry & Molecular Biology
Layla Mohammad Hadi, Elnaz Yaghini, Alexander J. MacRobert, Marilena Loizidou
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Oncology
Judith Pape, Tarig Magdeldin, Katerina Stamati, Agata Nyga, Marilena Loizidou, Mark Emberton, Umber Cheema
BRITISH JOURNAL OF CANCER
(2020)
Article
Oncology
Swethan Alagaratnam, Marilena Loizidou, Shi-yu Yang, Barry Fuller, Bala Ramesh
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2020)
Article
Multidisciplinary Sciences
Tayebeh Azimi, Marilena Loizidou, Miriam V. Dwek
SCIENTIFIC REPORTS
(2020)
Article
Oncology
Sami Znati, Rebecca Carter, Marcos Vasquez, Adam Westhorpe, Hassan Shahbakhti, Jessica Prince, Petra Vlckova, Chiara De Vellis, Zainab Bascal, Marilena Loizidou, Ricky A. Sharma
Article
Engineering, Biomedical
Linxiao Wu, Adrian Magaz, Suguo Huo, Arnold Darbyshire, Marilena Loizidou, Mark Emberton, Martin Birchall, Wenhui Song
ACTA BIOMATERIALIA
(2020)
Article
Multidisciplinary Sciences
Kurinchi S. Gurusamy, David Moher, Marilena Loizidou, Irfan Ahmed, Marc T. Avey, Carly C. Barron, Brian Davidson, Miriam Dwek, Christian Gluud, Gavin Jell, Kiran Katakam, Joshua Montroy, Timothy D. McHugh, Nicola J. Osborne, Merel Ritskes-Hoitinga, Kees van Laarhoven, Jan Vollert, Manoj Lalu
Summary: The study developed a tool to assess the clinical relevance of preclinical studies and refined and developed it using a modified Delphi consensus. The tool consists of eight domains and 28 signaling questions, providing a framework for researchers, journal editors, grant funders, and regulatory authorities to assess the potential clinical relevance of preclinical animal research.
Article
Biochemistry & Molecular Biology
Judith Pape, Katerina Stamati, Rawiya Al Hosni, Ijeoma F. Uchegbu, Andreas G. Schatzlein, Marilena Loizidou, Mark Emberton, Umber Cheema
Summary: The study utilized a biomimetic model including artificial cancer mass and fibrotic stromal compartment to mimic pancreatic cancer. The results showed varying drug sensitivity across different tumor models, with significantly reduced drug penetration in the complex tumor model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Inorganic & Nuclear
Mahshid Hashemkhani, Marilena Loizidou, Alexander J. MacRobert, Hawa Yagci Acar
Summary: This study demonstrated the one-step aqueous synthesis of simple AgInS2 QD compositions for the first time. The potential of these AIS QDs as effective drug carriers and in photodynamic therapy was proven through evaluation.
INORGANIC CHEMISTRY
(2022)
Review
Oncology
Jeries Abu-Hanna, Jigisha A. Patel, Evangelos Anastasakis, Richard Cohen, Lucie H. Clapp, Marilena Loizidou, Mohammad M. R. Eddama
Summary: This review examines and summarizes the therapeutic potential of inhibiting H3K27 demethylases, with a focus on the small-molecule inhibitor GSK-J4, based on pre-clinical in vitro and in vivo evidence.
CLINICAL EPIGENETICS
(2022)
Article
Biochemistry & Molecular Biology
Layla Mohammad Hadi, Katerina Stamati, Elnaz Yaghini, Alexander J. MacRobert, Marilena Loizidou
Summary: Photochemical internalisation (PCI) is a method that uses sub-lethal photodynamic therapy (PDT) to control cytosolic drug delivery, activating a photosensitiser with non-ionising visible light. This study examines the efficacy of PCI on ovarian cancer tumouroids using a porphyrin photosensitiser and a cytotoxin (Saporin), showing that PCI effectively decreases cancer cell viability within the central cancer mass and the surrounding stroma. Compared to treatment with Saporin or PDT alone, PCI significantly reduces stromal invasion by cancer cells. Tumouroids are suitable models for studying multi-cellular events after pharmacological assault.
Meeting Abstract
Surgery
Mohammad Eddama, Rijan Gurung, Konstantinos Fragkos, Richard Cohen, Marilena Loizidou, Lucie Clapp
BRITISH JOURNAL OF SURGERY
(2022)
Meeting Abstract
Oncology
U. Cheema, J. Pape, D. Bakkalci, M. Loizidou, K. Stamati, M. Emberton, S. Fedele
JOURNAL OF PATHOLOGY
(2022)
Article
Materials Science, Biomaterials
Fatma Demir Duman, Matej Sebek, Nguyen T K Thanh, Marilena Loizidou, Kaveh Shakib, Alexander J. MacRobert
JOURNAL OF MATERIALS CHEMISTRY B
(2020)
Article
Cell & Tissue Engineering
Katerina Stamati, Patricia A. Redondo, Agata Nyga, Joana B. Neves, Maxine G. B. Tran, Mark Emberton, Umber Cheema, Marilena Loizidou
JOURNAL OF TISSUE ENGINEERING
(2020)