Article
Biochemistry & Molecular Biology
Silvia Barbon, Elena Stocco, Martina Contran, Federico Facchin, Rafael Boscolo-Berto, Silvia Todros, Deborah Sandrin, Filippo Romanato, Piero Pavan, Veronica Macchi, Vincenzo Vindigni, Franco Bassetto, Raffaele De Caro, Andrea Porzionato
Summary: This study considers the use of cellular human diaphragm as a scaffold material for the treatment of volumetric muscle loss (VML). After decellularization through four detergent-enzymatic protocols, cells, DNA, and muscle fibers were effectively removed, while collagen/elastin and the majority of the glycosaminoglycan component were preserved. Adipose-derived stem cells cultured with decellularized samples demonstrated high viability, indicating the biosafety of the scaffolds. Acellular diaphragmatic grafts treated with SDS+Tergitol (TM) showed better collagen preservation and integrated well with host tissue upon implantation in mice.
Article
Engineering, Biomedical
Xiomara Fernandez-Garibay, Manuel Gomez-Florit, Rui M. A. Domingues, Manuela E. Gomes, Juan M. Fernandez-Costa, Javier Ramon-Azcon
Summary: Bioengineered human skeletal muscle tissues have been developed as in vitro systems for disease modeling. In this study, a-PL-based nanocomposite hydrogels were used as scaffolds to encapsulate human muscle satellite stem cells. The modulation of nanocrystal content facilitated the formation of highly aligned muscle fibers.
Article
Multidisciplinary Sciences
Sebastian Mathes, Alexandra Fahrner, Umesh Ghoshdastider, Hannes A. Ruediger, Michael Leunig, Christian Wolfrum, Jan Kruetzfeldt
Summary: The research indicates that FGF-2 plays a dual role in promoting muscle growth and intramuscular adipogenesis, and activates the miR29a/SPARC axis to regulate differentiation of fibro/adipogenic progenitors and intramuscular adipose tissue formation in aged skeletal muscle.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Surgery
Dominique Schaakxs, Mikael Wiberg, Paul J. Kingham, Daniel F. Kalbermatten
Summary: This study investigated the use of bioengineering techniques to repair peripheral nerve injuries and treat associated muscle atrophy. The results showed that the use of poly-3-hydroxybutyrate strips seeded with differentiated adipose stem cells promoted nerve repair and reduced muscle atrophy.
PLASTIC AND RECONSTRUCTIVE SURGERY
(2022)
Article
Engineering, Biomedical
Rafael Mestre, Nerea Garcia, Tania Patino, Maria Guix, Judith Fuentes, Mauricio Valerio-Santiago, Nuria Alminana, Samuel Sanchez
Summary: This study introduces a 3D-printed bioengineered human skeletal muscle platform, which efficiently models the three-dimensional structure of native tissue and plays a significant role in evaluating tissue differentiation, functionality, and force measurement. By assessing differentiation, maturation, force generation, contraction profiles, and changes in aging conditions, the flexibility of this platform for complex tissue modeling is validated.
Review
Pharmacology & Pharmacy
Zhanpeng Cui, Yang Jiao, Linyu Pu, James Zhenggui Tang, Gang Wang
Summary: Skeletal muscles have become a potential target for gene delivery and expression, with non-viral biomaterial-mediated methods showing promise for intramuscular gene delivery. Among these methods, polymers have shown significant progress and future potential in non-viral gene delivery materials.
Article
Engineering, Biomedical
Xiomara Fernandez-Garibay, Maria A. Ortega, Estefania Cerro-Herreros, Jordi Comelles, Elena Martinez, Ruben Artero, Juan M. Fernandez-Costa, Javier Ramon-Azcon
Summary: The article describes the development of the first in vitro 3D model of DM1 human skeletal muscle, which shows highly aligned myotubes from both healthy and DM1 cells. The 3D micropatterning significantly improved DM1 cell differentiation into multinucleated myotubes, suitable for drug testing. AntagomiR-23b treatment in bioengineered DM1 skeletal muscle microtissues demonstrates significant therapeutic effects in rescuing molecular DM1 hallmarks and structural phenotypes.
Article
Multidisciplinary Sciences
Rowan P. Rimington, Jacob W. Fleming, Andrew J. Capel, Patrick C. Wheeler, Mark P. Lewis
Summary: Current methods for studying the human neuromuscular junction (NMJ) lack physiological complexity, but this study provides methodologies for constructing 3D models of the human motor unit, enhancing transcription of motor neuron mRNAs. This work establishes functional human NMJs through bioengineering methods, demonstrating enhanced organization and physiology of the neuromuscular junction.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Kai Wang, Stephen H. H. Smith, Hirotaka Iijima, Zachary R. R. Hettinger, Adarsh Mallepally, Sanjeev G. G. Shroff, Fabrisia Ambrosio
Summary: Understanding the cell-autonomous changes in skeletal muscle during aging, regeneration, and intervention is crucial for improving functional recovery in the elderly. However, traditional biological aging models have limitations. To overcome these limitations, a 3D muscle aging system was created and it was found that old muscle constructs (OMC) exhibit a sarcopenic phenotype and impaired regenerative capacity. Enhanced expression of complement component 4b (C4b) in aging muscle was identified as a contributing factor to impaired muscle regeneration, but inhibition of C4b improved regeneration in vitro and in vivo, suggesting a novel approach to enhance aged muscle repair.
ADVANCED MATERIALS
(2023)
Article
Physiology
Christopher G. Vann, Xin Zhang, Alastair Khodabukus, Melissa C. C. Orenduff, Yu-Hsiu Chen, David L. L. Corcoran, George A. A. Truskey, Nenad Bursac, Virginia B. B. Kraus
Summary: Exercise-mimetic contractile activity of human engineered muscle affects the expression of miRs and the number of secreted EVs, providing new insights into the role of exercise in organ-organ interactions.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Engineering, Biomedical
Shuming Sun, Han Liu, Yan Hu, Yanpeng Wang, Mingri Zhao, Yijun Yuan, Yafei Han, Yingying Jing, Jin Cui, Xiaoxiang Ren, Xiao Chen, Jiacan Su
Summary: In this study, human skeletal muscle-specific ssDNA aptamers were selected and identified using SELEX. The aptamers showed preferential interaction with human skeletal muscle cells in vitro and specifically targeted these cells in vivo. The formation of a disulfide bond with nanoliposomes did not affect the ability of the aptamers to target skeletal muscle cells. This newly identified ssDNA aptamer and nanoliposome modification have potential applications in the treatment of human skeletal muscle diseases.
BIOACTIVE MATERIALS
(2023)
Article
Nutrition & Dietetics
Yusuke Komiya, Makoto Sugiyama, Masaru Ochiai, Nanako Osawa, Yuto Adachi, Shugo Iseki, Keizo Arihara
Summary: The study found that feeding a diet containing olive oil can enhance the endurance of mice and increase the accumulation of intramuscular triacylglycerol. This increased endurance is independent of muscle fiber type. Additionally, no metabolic impairments or differences in growth profiles were observed in mice fed with the olive oil diet compared to the controls.
Article
Multidisciplinary Sciences
Mengmeng Zhang, Lu Wang, Jinyao Liu, Yan Pang
Summary: In this study, envelope virus-mimetic nanovaccines were developed by combining bioengineered cell membranes with bacterial vesicles. These nanovaccines showed enhanced immune responses and biosafety, making them a promising platform for antiviral vaccine development.
Article
Cell Biology
Christine T. Nguyen, Majid Ebrahimi, Penney M. Gilbert, Bryan A. Stewart
Summary: This study analyzed the electrophysiological characteristics of healthy and diseased three-dimensional bioengineered skeletal muscle tissues, focusing on Duchenne muscular dystrophy. The research also tested a potential therapeutic compound and showed improvement in membrane potential in dystrophic muscle, laying the foundation for examining potential therapies for muscular disorders in vitro.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Xin Zheng Liu, Zhi Juan Wen, Yun Meng Li, Wan Ru Sun, Xiao Qian Hu, Jia Zhi Zhu, Xin Yu Li, Ping Yu Wang, Jose Luis Pedraz, Jung-Hwan Lee, Hae-Won Kim, Murugan Ramalingam, Shuyang Xie, Ranran Wang
Summary: In this study, a drug-free bacteria-derived outer membrane vesicle (OMV) functionalized Fe3O4-MnO2 (FMO) nanoplatform was developed for neutrophil-mediated targeted delivery and photothermally enhanced cancer immunotherapy. The modified OMVs enhanced the accumulation of FMO NPs at the tumor site and induced immunogenic cell death (ICD) and regulated the tumor hypoxia environment. Additionally, OMVs effectively activated immune cells and the photothermal therapy (PTT) caused by MnO2 and Fe3O4 stimulated systemic immunity and promoted the enrichment of neutrophil-equipped nanoparticles. This multi-modal treatment system with targeted delivery capability achieved effective tumor immunotherapy and prevention of tumor recurrence.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Medicine, Research & Experimental
Prasad D. Trivedi, Chenghui Yu, Payel Chaudhuri, Evan J. Johnson, Tina Caton, Laura Adamson, Barry J. Byrne, Nicole K. Paulk, Nathalie Clement
Summary: The study optimized and compared the production of rAAV in suspension HEK293 cells by PEI-mediated transfection and HSV infection. The HSV production system yielded higher rAAV titers and full capsid percentages, but contained detectable levels of HSV DNA. The potency of rAAV stocks produced by both methods was found to be identical.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2022)
Article
Medicine, Research & Experimental
Marti Cabanes-Creus, Renina Gale Navarro, Erhua Zhu, Grober Baltazar, Sophia H. Y. Liao, Matthieu Drouyer, Anais K. Amaya, Suzanne Scott, Loan Hanh Nguyen, Adrian Westhaus, Matthias Hebben, Laurence O. W. Wilson, Adrian J. Thrasher, Ian E. Alexander, Leszek Lisowski
Summary: Recent clinical successes have increased interest in using adeno-associated virus (AAV) vectors for therapeutic gene delivery. This study bioengineered a new generation of AAV vectors, called AAV-SYDs, with increased human hepatotropism. By employing directed evolution and domain-swapping strategies, the key capsid residues responsible for enhanced primary human hepatocyte uptake and transgene expression were identified and studied. These findings highlight the potential of AAV-SYDs as liver gene therapy vectors and provide insights into their enhanced transduction profile.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2022)
Article
Cell Biology
Ronnie Blazev, Christian S. Carl, Yaan-Kit Ng, Jeffrey Molendijk, Christian T. Voldstedlund, Yuanyuan Zhao, Di Xiao, Andrew J. Kueh, Paula M. Miotto, Vanessa R. Haynes, Justin P. Hardee, Jin D. Chung, James W. McNamara, Hongwei Qian, Paul Gregorevic, Jonathan S. Oakhill, Marco J. Herold, Thomas E. Jensen, Leszek Lisowski, Gordon S. Lynch, Garron T. Dodd, Matthew J. Watt, Pengyi Yang, Bente Kiens, Erik A. Richter, Benjamin L. Parker
Summary: This study identified divergent and common signaling networks during and after different exercise modalities through phosphoproteomic analysis. It also revealed C18ORF25 as a regulator of exercise signaling and muscle function.
Article
Biochemistry & Molecular Biology
Jinbiao Chen, Yang Zhao, Fan Zhang, Jia Li, Jade A. Boland, Ngan Ching Cheng, Ken Liu, Jessamy C. Tiffen, Patrick Bertolino, David G. Bowen, Andreas Krueger, Leszek Lisowski, Ian E. Alexander, Mathew A. Vadas, Emad El-Omar, Jennifer R. Gamble, Geoffrey W. McCaughan
Summary: MiR-181 expression is increased in hepatocellular carcinoma, and it plays important roles in tumorigenesis and regulating immune and vascular cells. Knockout of miR-181 reduces the size and number of liver tumors and partially reverses epithelial-mesenchymal transition in tumors. Up-regulation of CBX7 expression is involved in the tumor-suppressive effects of miR-181.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Review
Cell Biology
Anne Brunet, Margaret A. Goodell, Thomas A. Rando
Summary: Stem cell function declines during ageing, resulting in the loss of tissue integrity and health deterioration. This Review discusses the mechanisms underlying ageing in stem cells and their niches, and potential rejuvenation strategies.
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2023)
Article
Gastroenterology & Hepatology
Nadja Meumann, Marti Cabanes-Creus, Moritz Ertelt, Renina Gale Navarro, Julie Lucifora, Qinggong Yuan, Karin Nien-Huber, Ahmed Abdelrahman, Xuan-Khang Vu, Liang Zhang, Ann-Christin Franke, Christian Schmithals, Albrecht Piiper, Annabelle Vogt, Maria Gonzalez-Carmona, Jochen T. Frueh, Evelyn Ullrich, Philip Meuleman, Steven R. Talbot, Margarete Odenthal, Michael Ott, Erhard Seifried, Clara T. Schoeder, Joachim Schwable, Leszek Lisowski, Hildegard Buning
Summary: This article reports two gene vector variants, MLIV.K and MLIV.A, which were obtained through in vivo AAV peptide display selection in mice. They showed improved hepatocyte targeting and transduction efficiency compared to AAV2 and AAV8, and have potential for liver disease treatment.
Article
Medicine, Research & Experimental
Francesco Puzzo, Chuanling Zhang, Bethany Powell Gray, Feijie Zhang, Bruce A. Sullenger, Mark A. Kay
Summary: This study developed a new chemically modified AAV vector, N & epsilon;-AAVs, which allows post-production engineering through click chemistry. The functionalized N & epsilon;-AAVs showed increased transduction efficiency and demonstrated selective uptake in vivo, making them versatile gene therapy vectors for specific cell targeting.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Review
Chemistry, Medicinal
Jessica Merjane, Roger Chung, Rickie Patani, Leszek Lisowski
Summary: Despite the mysterious etiology, differentiated treatments are required for ALS to address both familial and sporadic cases. Targeting mechanisms of defective protein homeostasis and RNA processing, as well as exploring the use of gene therapy through adeno-associated virus (AAV) for gene delivery to the CNS, might provide potential therapeutic interventions. Overall, there is a strong need for disease modifying treatments for ALS that can effectively treat the full spectrum of cases.
MEDICINAL RESEARCH REVIEWS
(2023)
Letter
Biochemical Research Methods
Andrew D. Holmes, Patricia P. Chan, Qi Chen, Pavel Ivanov, Laurence Drouard, Norbert Polacek, Mark A. Kay, Todd M. Lowe
Review
Cell Biology
Antoine de Morree, Thomas A. Rando
Summary: Adult stem cells are crucial for maintaining and regenerating mammalian tissues. The quiescent state of stem cells, controlled by various post-transcriptional mechanisms, plays a vital role in their long-term maintenance and ability to respond to injuries. Understanding these mechanisms has important implications for regenerative medicine.
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2023)
Article
Gastroenterology & Hepatology
Ngee-Soon Lau, Mark Ly, Claude Dennis, Kasper Ewenson, Hayden Ly, Joanna L. Huang, Marti Cabanes-Creus, Sumon Chanda, Chuanmin Wang, Leszek Lisowski, Ken Liu, James Kench, Geoffrey McCaughan, Michael Crawford, Carlo Pulitano
Summary: Liver splitting during normothermic machine perfusion has the potential to revolutionize split liver transplantation. We developed a technique to reliably achieve two grafts from a single donor liver, allowing for semi-elective transplantation and sophisticated graft assessment prior to implant. This method successfully split 10 whole livers into 20 partial grafts, with a high rate of graft survival.
Article
Multidisciplinary Sciences
Adriana Gonzalez-Sandoval, Katja Pekrun, Shinnosuke Tsuji, Feijie Zhang, King L. Hung, Howard Y. Chang, Mark A. Kay
Summary: The effectiveness of rAAV vectors varies between species, and a single amino acid change in the vector can alter its epigenomic state and transgene expression levels between species.
NATURE COMMUNICATIONS
(2023)
Meeting Abstract
Biotechnology & Applied Microbiology
Calvin J. Stephens, Katja Pekrun, Jianpeng Xu, Shinnosuke Tsuji, Yuqing Jing, Francesco Puzzo, Feijie Zhang, Mark A. Kay
Meeting Abstract
Biotechnology & Applied Microbiology
Adriana Gonzalez-Sandoval, Shinnosuke Tsuji, King L. Hung, Feijie Zhang, Mark A. Kay
Meeting Abstract
Biotechnology & Applied Microbiology
Hagoon Jang, Kirk Chu, Feijie Zhang, Calvin Stephens, Mark A. Kay
