Article
Biochemistry & Molecular Biology
Julie Qiaojin Lin, Deepak Khuperkar, Sofia Pavlou, Stanislaw Makarchuk, Nikolaos Patikas, Flora C. Y. Lee, Julia M. Zbiegly, Jianning Kang, Sarah F. Field, David M. D. Bailey, Joshua L. Freeman, Jernej Ule, Emmanouil Metzakopian, Marc-David Ruepp, Giovanna R. Mallucci
Summary: Enhanced expression of the cold-shock protein RBM3 is neuroprotective. A CRISPR-Cas9 knockout screen identified splicing factors that regulate RBM3 levels, with HNRNPH1 being the strongest positive regulator. Moderate hypothermia represses inclusion of a poison exon via HNRNPH1's thermosensitive interaction with a G-rich motif. The study provides mechanistic insights into RBM3 regulation and potential therapeutic targets for neuroprotection.
Article
Biology
Diego Peretti, Heather L. Smith, Nicholas Verity, Ibrahim Humoud, Lis de Weerd, Dean P. Swinden, Joseph Hayes, Giovanna R. Mallucci
Summary: Increasing levels of the cold-shock protein RBM3, either through cooling or ectopic over-expression, can prevent synapse and neuronal loss in mouse models of neurodegeneration. RBM3 mediates structural plasticity through a distinct, non-canonical activation of TrkB signaling, and TrkB signaling is necessary for RBM3 induction and related neuroprotective effects.
LIFE SCIENCE ALLIANCE
(2021)
Article
Biochemistry & Molecular Biology
Xin Yuan, Jun Zhang, Ting-Ting Ma, Rui-Juan Zhuang, Bing-Bing Lei, Lei Wang, Bin-Feng Cheng, Mian Wang, Hai-Jie Yang
Summary: The FAK/Src signaling axis plays a crucial role in regulating the transcription of the Rbm3 gene and mediating the neuroprotective effects of mild hypothermia. Inhibition of FAK/Src significantly abrogates the induction of RBM3 by hypothermia, leading to a loss of neuroprotective effects against rotenone in SH-SY5Y cells.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Qi Xiao, Yuan Liu, XingJian Zhang, ZhongZhong Liu, JianSheng Xiao, QiFa Ye, BiQi Fu
Summary: Mild hypothermia protects the liver from ischemia reperfusion-induced injury by upregulating RBM3 expression, reducing p38 and JNK signaling pathway activation, oxidative stress injury, and apoptosis.
Article
Medicine, General & Internal
Haibin Dai, Yan Zhou, Yue Lu, Xiangsheng Zhang, Zong Zhuang, Yongyue Gao, Guangjie Liu, Chunlei Chen, Jin Ma, Wei Li, Chunhua Hang
Summary: Early brain injury is a major factor contributing to the poor prognosis of subarachnoid hemorrhage (SAH) patients. Cold-inducible RNA-binding protein (CIRP) has been implicated in ischemic brain injury, and its role in SAH treatment needs further evaluation. This study found that hypothermia treatment reduced brain tissue damage, apoptosis, and inflammatory signaling induced by SAH in a rat model.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Immunology
Soo Yeon Kim, Hyo-Jeong Ban, Siwoo Lee, Hee-Jeong Jin
Summary: This study reveals that genetic factors related to temperature sensitivity can regulate the expression and function of CIRP, and provides valuable insights into predicting potential diseases through analysis of inherent genetic factors in humans.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Medicine, Research & Experimental
Marco Preussner, Heather L. Smith, Daniel Hughes, Min Zhang, Ann-Kathrin Emmerichs, Silvia Scalzitti, Diego Peretti, Dean Swinden, Alexander Neumann, Tom Haltenhof, Giovanna R. Mallucci, Florian Heyd
Summary: By removing a poison exon in the RBM3 gene, high levels of RBM3 expression can be achieved in mouse brains independent of cooling, resulting in remarkable neuroprotection in prion-diseased mice.
EMBO MOLECULAR MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Amit Chaudhary, Pankaj Kumar Chaurasia, Sandeep Kushwaha, Pallavi Chauhan, Aakash Chawade, Ashutosh Mani
Summary: Cold shock proteins are a conserved family of proteins that play important roles in low-temperature stress response and nucleic acid binding in bacteria. They are involved in various cellular and metabolic processes in prokaryotes and have been found to be crucial in the progression of cancer and Alzheimer's disease in humans.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Cell Biology
Yajie Hu, Yang Liu, Xin Quan, Wenxuan Fan, Bin Xu, Shize Li
Summary: This review article provides insights into the important physiological mechanism of RNA-binding motif protein 3 (RBM3) in response to cold stress and its regulatory role in various cellular processes. It also discusses the cytoprotective mechanism of RBM3 under stress conditions, its transcriptional regulation, neuroprotective effects, as well as its oncogenic role and controversy in cancer.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Immunology
Kehong Chen, Atsushi Murao, Adnan Arif, Satoshi Takizawa, Hui Jin, Jianxin Jiang, Monowar Aziz, Ping Wang
Summary: In sepsis, eCIRP-induced NETs inhibit phagocytosis of apoptotic cells by macrophages through neutrophil elastase-dependent reduction of integrins. This discovery reveals a potential mechanism of therapeutic failure.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Entomology
Asli Dageri, Mohammed Lengichow Kadir, Nurper Guz, Ayhan Ogreten, Muhammad Arshad
Summary: In this study, two cold-regulated genes, TgAFP and TgCSDP, were identified in T. granarium. The expression of these genes in response to temperature and cold stress was examined. The results suggest that these genes play crucial roles in the cold survival and adaptation of T. granarium.
JOURNAL OF STORED PRODUCTS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Sayantani Roy, Soumendu Boral, Snigdha Maiti, Tushar Kushwaha, Aditya J. Basak, Woonghee Lee, Amit Basak, Shivajirao L. Gholap, Krishna K. Inampudi, Soumya De
Summary: RBM3 is a cold-shock protein that plays important roles in global protein synthesis, cell proliferation, and apoptosis. The N-terminal RRM domain of RBM3 forms a beta alpha beta beta alpha beta topology, with key interactions between RNA and the RRM domain involving hydrogen bonds, pi-pi, and pi-cation interactions. NMR studies reveal that RBM3 forms oligomers in solution, which are favored by lower temperatures, and the oligomerization of the RRM domain occurs via nonspecific interactions. This study provides detailed structural insights into RBM3 and its temperature-dependent oligomerization mechanism.
Review
Immunology
Jingrun Han, Yibo Zhang, Peng Ge, Tikam Chand Dakal, Haiyun Wen, Shuangfeng Tang, Yalan Luo, Qi Yang, Bianca Hua, Guixin Zhang, Hailong Chen, Caiming Xu
Summary: Cold-inducible RNA-binding protein (CIRP) is an intracellular stress-response protein that responds to various stress stimuli by altering its expression and mRNA stability. It gets translocated from the nucleus to the cytoplasm through methylation modification and stored in stress granules. It can also be secreted out of cells through the lysosomal pathway as Extracellular CIRP (eCIRP).
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Clinical Neurology
Mingming Li, Min Yao, Kangmei Shao, Xueyang Shen, Zhaoming Ge, Yongnan Li
Summary: This study found that the serum levels of cold-inducible RNA-binding protein (CIRP) are elevated in patients with acute ischemic stroke (AIS) and are associated with the severity and prognosis of the disease. The serum CIRP levels were significantly correlated with the severity of cerebral infarction and poor prognosis. Therefore, serum CIRP levels can serve as an important biomarker for assessing brain injury in AIS patients.
FRONTIERS IN NEUROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Daiki Jingu, Mika Iino, Joji Kawasaki, Eriko Urano, Shinya Kusakari, Yuriko Hayashi, Takashi Matozaki, Hiroshi Ohnishi
Summary: SIRPα is a cold stress-responsive signaling molecule in neurons, and its phosphorylation is involved in regulating behavioral changes in mice. The phosphorylation of SIRPα induced by cold stress is related to the activity of protein tyrosine phosphatases (PTPases).
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)