Controlling the optimum dose of AMPTS functionalized-magnetite nanoparticles for hyperthermia cancer therapy

Magnetic hyperthermia has been used for many years to treat a variety of malignant tumors. One of the problems in magnetic hyperthermia is the choice of the correct particle concentration to achieve a defined temperature increase in the tumor tissue. In this study, we evaluated magnetic heat distribution induced by Fe3O4-APTMS magnetic nanoparticles in agar tissue phantom when it subjected to the AC magnetic filed. Using the correct nanoparticle dosage and considering their specific loss power, it is possible to estimate the efficiency of this therapeutic method. The experimental data were compared with a computer-based model, which were created using COM-SOL Multiphysics to simulate the heat dissipation within the tissue for typical configurations of the tumor position as well as particle distribution within the tumor. Heating the cancer cells up to 50 degrees C for 10 min was sufficient for complete cell killing and the heat dose of 19.9 W/g(tissue) is required for 5-mm tumor. Cell viability assay showed that MNPs exhibited no significant cytotoxicity against HeLa cells. Additionally, it was observed that the FITC-labeled Fe3O4-APTMS MNPs presented high cell biocompatibility and cellular uptake for efficient endocytosis.