Article
Chemistry, Multidisciplinary
Maylis Boitet, Hyeju Eun, Asma Achek, Virginia Carla de Almeida Falcao, Vincent Delorme, Regis Grailhe
Summary: Biolum' RGB is a highly customizable bioluminescence imager that uses a consumer color digital camera and a high-aperture lens to quantify cell bioluminescence radiation and distinguish spectral signatures from different bioluminescence probes.
Article
Biochemistry & Molecular Biology
Adam L. Valkovic, Martina Kocan, Brad Hoare, Sarah Marshall, Daniel J. Scott, Ross A. D. Bathgate
Summary: This article introduces a new BRET-based cGMP sensor called CYGYEL, which enables efficient and rapid detection of cGMP activity in plate-based assays and successfully detects cGMP signals in different cell types.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
H. Dacres, F. Weihs, J. Wang, A. Anderson, S. C. Trowell
Summary: A novel bioluminescence resonance energy transfer (BRET)-based biosensor has been developed to measure bacterial protease activity in milk. This biosensor is highly selective for bacterial protease activity and is more sensitive and faster than the established FITC-Casein method. It is suitable for use in production settings.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Mitsuru Hattori, Nae Sugiura, Tetsuichi Wazawa, Tomoki Matsuda, Takeharu Nagai
Summary: Hemostasis is crucial for tissue repair and organism survival. Periodic blood tests are important to prevent diseases from blood coagulation abnormalities. A novel ratiometric bioluminescent indicator, Thrombastor, was developed to reflect thrombin cleavage activity, offering faster detection and effective diagnosis for coagulation disorders.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Elbegduuren Erdenee, Alice Y. Ting
Summary: LuCID is a novel calcium indicator and transcriptional calcium integrator that can real-time read out calcium dynamics in cells and convert the signal into stable gene expression. Its modular design enables it to read out other cellular events, making it an important tool for studying cells and cell populations utilizing calcium signaling.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xiaoyuan Ma, Meichun Gao, Henry F. Vischer, Rob Leurs
Summary: Conformational biosensors are useful tools in pharmacology to monitor the activation state of receptor proteins. The study presents a NanoBRET-based conformational biosensor for the histamine H3R receptor, allowing the detection of ligand efficacy on living cells. It also explores the biosensor's compatibility with photopharmacological ligands and its potential use in membrane preparations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Alessandro De Simone, Maya N. Evanitsky, Luke Hayden, Ben D. Cox, Julia Wang, Valerie A. Tornini, Jianhong Ou, Anna Chao, Kenneth D. Poss, Stefano Di Talia
Summary: Regeneration is a complex process that restores tissue to its original size and shape. In the case of regenerating zebrafish scales, rhythmic travelling waves of Erk activity control bone growth in time and space, propagating as trigger waves that traverse the entire scale and regulate the rate of regeneration. Synchronous activation of Erk in place of travelling waves impairs tissue growth, highlighting the importance of wave-distributed Erk activation in regeneration.
Article
Multidisciplinary Sciences
Katarina Nemec, Hannes Schihada, Gunnar Kleinau, Ulrike Zabel, Eugene O. Grushevskyi, Patrick Scheerer, Martin J. Lohse, Isabella Maiellaro
Summary: This study discovered that receptor-activity-modifying protein 2 (RAMP2) acts as a specific allosteric modulator of parathyroid hormone 1 receptor (PTH1R), shifting it to a preactivated state and affecting its downstream signaling. These findings provide a new avenue for modulating GPCR function and drug design.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
M. Fethullah Simsek, Angad Singh Chandel, Didar Saparov, Oriana Q. H. Zinani, Nicholas Clason, Ertugrul M. Ozbudak
Summary: This study developed precise reporters to measure the clock and ppErk gradient in zebrafish. It found that the Her1-Her7 oscillator drives segmental commitment by periodically lowering ppErk, therefore projecting its oscillation onto the ppErk gradient. Pulsatile inhibition of the ppErk gradient can fully substitute for the role of the clock, and kinematic clock waves are dispensable for sequential segmentation.
Article
Chemistry, Multidisciplinary
Dan Chen, Chunqiong Wang, Dezhi Yang, Huimin Deng, Qiulan Li, Li Chen, Gaokun Zhao, Junli Shi, Ke Zhang, Yaling Yang
Summary: In this study, a novel smartphone-assisted sensing platform for the rapid and onsite detection of glyphosate in tobacco products has been successfully proposed. The platform utilizes the peroxidase-like activity of Fe3O4-based nanozyme for the detection, and has the advantages of fast response, portability, and high selectivity. It has potential applications in onsite screening of glyphosate in actual samples.
Article
Multidisciplinary Sciences
Godfrey Dzhivhuho, Jordan Holsey, Ethan Honeycutt, Heather O'Farrell, David Rekosh, Marie-Louise Hammarskjold, Patrick E. H. Jackson
Summary: During HIV infection, the transport of viral mRNA from the cell nucleus to the cytoplasm is facilitated by Rev protein and the Rev Response Element (RRE). The activity of Rev protein, which varies among different primary isolates, is determined by amino acid differences within the oligomerization domain. Small sequence changes can modulate Rev-RRE activity, potentially impacting HIV pathogenesis.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Hyunju Cho, Chang-Seuk Lee, Tae Hyun Kim
Summary: A label-free electrochemical biosensor was proposed for monitoring protein kinase activity and inhibition using a peptide-modified electrode. The sensor showed reliable, sensitive, and selective detection of PKA activity with a wide dynamic range and high selectivity, making it possible to evaluate the activity and inhibition of PKA in real samples.
Article
Microbiology
Yuting Shi, Jingwan Han, Bo Zhu, Zhi Liu, Qingmiao Liang, Chunlin Lan, Zhengyang Li, Hanping Li, Yongjian Liu, Lei Jia, Tianyi Li, Xiaolin Wang, Jingyun Li, Bohan Zhang, Junjun Jiang, Lin Li
Summary: This study identifies the subtype-specific loci of HIV-1 subtype B RRE circulating in China and examines their impact on viral replication and Rev-RRE activity.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Oncology
Joanne M. Munck, Valerio Berdini, Luke Bevan, Jessica L. Brothwood, Juan Castro, Aurelie Courtin, Charlotte East, Roberta Ferraldeschi, Tom D. Heightman, Christopher J. Hindley, Justyna Kucia-Tran, John F. Lyons, Vanessa Martins, Sandra Muench, Christopher W. Murray, David Norton, Marc O'Reilly, Michael Reader, David C. Rees, Sharna J. Rich, Caroline J. Richardson, Alpesh D. Shah, Lukas Stanczuk, Neil T. Thompson, Nicola E. Wilsher, Alison J-A Woolford, Nicola G. Wallis
Summary: ASTX029 is a highly potent and selective dual-mechanism ERK inhibitor that targets MAPK-activated cancers by inhibiting both ERK catalytic activity and phosphorylation by MEK. It has shown significant antitumor activity in both in vitro and in vivo models, including acquired resistance to MAPK pathway inhibitors. ASTX029 is currently undergoing evaluation in a clinical trial for patients with advanced solid tumors.
MOLECULAR CANCER THERAPEUTICS
(2021)
Article
Oncology
Sara J. Hamis, Yury Kapelyukh, Aileen McLaren, Colin J. Henderson, C. Roland Wolf, Mark A. J. Chaplain
Summary: The study developed a mechanistic mathematical model to describe the synergistic action of dabrafenib and trametinib on ERK activity in BRAFV600E-mutant melanoma cells, elucidating the molecular mechanism underlying vertical inhibition of the BRAF-MEK-ERK cascade.
BRITISH JOURNAL OF CANCER
(2021)