Article
Neurosciences
Wonchul Shin, Lisi Wei, Gianvito Arpino, Lihao Ge, Xiaoli Guo, Chung Yu Chan, Edaeni Hamid, Oleg Shupliakov, Christopher K. E. Bleck, Ling-Gang Wu
Summary: This study reveals that endocytosis is mainly mediated by calcium-triggered and dynamin-mediated closure of Omega profiles and fusion pores, leading to the formation of Lambda-shaped, Omega-shaped, and O-shaped vesicles, rather than the traditional flat-to-round membrane transformation.
Article
Computer Science, Interdisciplinary Applications
Tiankui Zhang, Charles W. Wolgemuth
Summary: The dynamics of thin, membrane-like structures are crucial in cell biology, but simulating large scale deformations remains challenging due to spatial heterogeneity. A general computational framework has been developed to simulate the dynamics of membranes and vesicles, successfully predicting equilibrium shapes of multiphase vesicles.
JOURNAL OF COMPUTATIONAL PHYSICS
(2022)
Article
Environmental Sciences
Meng Yang, Wen-Xiong Wang
Summary: This study quantified the nonspecific interactions between three different functional polystyrene nanoplastics and zebrafish cells. The uptake of the nanoplastics in the cells peaked within 2 hours and then decreased, and the uptake rate was similar among the different types of nanoplastics but related to their functionality. The clathrin-mediated and caveolae-mediated pathways were mainly involved in the uptake process. The internalized nanoplastics were transported to lysosomes and exocytosed by the cells via energy-dependent processes. The estimated retention half-lives of the nanoplastics in fish cells were also determined.
ENVIRONMENTAL POLLUTION
(2023)
Article
Biochemistry & Molecular Biology
Takahito Moriyama, Fumio Hasegawa, Yoei Miyabe, Kenichi Akiyama, Kazunori Karasawa, Keiko Uchida, Kosaku Nitta
Summary: The study reveals that albumin in podocytes is transported intracellularly through caveolae, entering cells with Fc receptors, moving along actin cytoskeleton, and eventually being degraded or exocytosed.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Engineering, Environmental
Gwang-Bum Im, Jaeyoung Lee, Jiwoo Song, Taekyung Yu, Suk Ho Bhang
Summary: Cu-AMNs have advantages in cell treatment such as low mitochondrial damage, reduced ATP consumption, decreased cytotoxicity, mild reactive oxygen species generation, enhanced angiogenic paracrine factor secretion, and improved HIF1 alpha expression. The transplantation of Cu-AMN-treated hADSCs led to a significant increase in angiogenesis and blood perfusion in a mouse hindlimb ischemia model compared with conventional hADSC therapy.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Nanoscience & Nanotechnology
Jiapei Yang, Li Xu, Ling Di, Yue Su, Xinyuan Zhu
Summary: PEG, as the gold standard for stealth polymer materials, is widely used in drug delivery due to its excellent properties. However, the lack of understanding about the fate of PEG at the cellular level has hindered its application in diagnosis and therapy. Research showed that PEG could be internalized into cells in 1 hour, localized to lysosomes, cytosol, ER, and mitochondria. The fate of PEG in cells could be regulated by conjugating different small molecules, which is crucial for the rational design of PEGylation for drug delivery.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biology
Ana M. Cadenas, Luciana Gallo, Fernando D. Marengo
Summary: This review discusses the role of the cortical actin network in secretory cells, including its involvement in regulating plasmalemma tension, organizing membrane protein microdomains, remodeling the cell surface, and driving vesicle motion.
Review
Endocrinology & Metabolism
Xinquan Yang, Weifeng Tu, Xuzhu Gao, Qi Zhang, Jinping Guan, Junlong Zhang
Summary: The mechanism of neuroendocrine cell exocytosis is regulated by multiple factors, including SNARE protein complexes, proteins, lipids, protein kinases, drugs, and toxins. Syntaxin-1 plays a crucial role in neuroendocrine cell exocytosis, and the accurate and rapid binding of syntaxin-1 to other SNARE proteins promotes membrane fusion. Investigating syntaxin-1 mutation-associated diseases could provide novel mechanistic therapeutic targets for the treatment of neuroendocrine disorders.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Liang Zhang, Xinlin Liang, Tomas Takac, George Komis, Xiaojuan Li, Yuan Zhang, Miroslav Ovecka, Yanmei Chen, Jozef Samaj
Summary: In plants, membrane compartmentalization relies on vesicle trafficking for organelle communication. Membrane proteins involved in vesicle trafficking are dynamic and responsive to environmental changes and cellular signals. Therefore, understanding the localization and dynamics of these proteins is crucial for dissecting vesicular trafficking pathways.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Multidisciplinary Sciences
Wonchul Shin, Ben Zucker, Nidhi Kundu, Sung Hoon Lee, Bo Shi, Chung Yu Chan, Xiaoli Guo, Jonathan T. Harrison, Jaymie Moore Turechek, Jenny E. Hinshaw, Michael M. Kozlov, Ling-Gang Wu
Summary: The process of membrane budding is essential for cell survival, but the forces involved in non-coated membrane budding have remained unclear. This study reveals how non-coated membrane budding is mediated, involving actin filaments and dynamin to generate the necessary forces. These mechanisms control the speed, size, and number of vesicles formed, and have implications for various membrane remodeling processes.
NATURE COMMUNICATIONS
(2022)
Article
Environmental Sciences
Ling Liu, Kexin Xu, Bowen Zhang, Yiyuan Ye, Qiu Zhang, Wei Jiang
Summary: Microplastics and nanoplastics, specifically 50 nm and 500 nm polystyrene particles, can be internalized into living cells through passive membrane penetration and active endocytosis, accumulating mainly in lysosomes. Larger particles, like 5 mu m polystyrene particles, are unable to adhere to or be internalized by cells due to their size and weak Brownian motion.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biochemistry & Molecular Biology
Noa Barak-Broner, Dafna Singer-Lahat, Dodo Chikvashvili, Ilana Lotan
Summary: In this study, we found that the polybasic juxtamembrane region of syntaxin1A acts as a fusion clamp in PC12 cells and is required for depolarization-induced Ca2+-dependent opening of Syx. Phosphorylation of Syx at Ser-14 by casein kinase 2 was identified as a key molecular determinant for this process. The S14 phosphorylation confers the requirement for the close-to-open transition of Syx, which is crucial for both Ca2+-triggered release and spontaneous release.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Yan Zhen, Maja Radulovic, Marina Vietri, Harald Stenmark
Summary: The compartmentalization of eukaryotic cells is essential for their viability, with membranes forming boundaries between organelles and the cytosol. Cellular membranes can be compromised by various factors, but there are evolved mechanisms like membrane scission and endocytosis to maintain membrane integrity.
Review
Biochemistry & Molecular Biology
Joseph S. Erlichman, James C. Leiter
Summary: Metal oxide nanoparticles have potential as theranostic agents, but the complexity of their chemistry as they interact with different environments and proteins may hinder their clinical translation. Inadequate translational models and discrepancies between simplified experiments and biological activities in organisms pose challenges for accurately predicting the therapeutic potential of metal oxide NPs. Understanding the evolving pharmacology of these NPs in biological systems is crucial for establishing effective translational test systems.
Article
Cell Biology
Natsuko Tsurudome, Yuji Minami, Katsuko Kajiya
Summary: This study aimed to investigate the action point of SPC around the plasma membranes and the involvement of fisetin. The results showed that SPC entered cells through endocytosis and did not undergo transcytosis, and SPC-induced contracting cells produced exosomes. Moreover, fisetin prevented contractions by directly targeting vascular smooth muscle cells.