Article
Infectious Diseases
Zeyi Liang, Jiahao Shen, Jing Liu, Qinfan Li, Feng Yang, Xuezhi Ding
Summary: This study aimed to investigate the effects of ascorbic acid on antibiotic susceptibility of major bovine mastitis pathogens. The results showed that ascorbic acid could decrease the susceptibility of penicillin and ampicillin, while increase the susceptibility of erythromycin, kanamycin, streptomycin, and ciprofloxacin. Moreover, ascorbic acid specifically reduced the susceptibility of tetracycline for gram-positive bacteria and chloramphenicol for gram-negative bacteria.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Biology
Shigenobu Kasai, Ankush Prasad, Ryoma Kumagai, Keita Takanohashi
Summary: In this study, a portable electrochemical device was developed as a novel method for diagnosing mastitis. By measuring the difference in oxygen concentration and the number of somatic cells, mastitis can be accurately diagnosed.
Article
Horticulture
Siddharood Maragal, Eguru Sreenivasa Rao, D. C. Lakshmana Reddy
Summary: This study investigated the genes/QTLs governing important fruit quality traits in watermelon, particularly focusing on fruit shape and flesh color. Through QTL mapping and candidate gene analysis, several major QTLs and potential candidate genes were identified. The results provide valuable information for future functional validation and breeding of important fruit quality traits in watermelon.
SCIENTIA HORTICULTURAE
(2023)
Article
Veterinary Sciences
Zul I. Huma, Neelesh Sharma, Savleen Kour, Sung Jin Lee
Summary: Mastitis is a complex disease that negatively affects the quality and quantity of milk in dairy cows. Staphylococcus aureus and Escherichia coli are the main pathogens involved in mastitis, along with other environmental pathogens. The somatic cell count is directly related to the severity of mastitis and has a significant negative correlation with fat, lactose, solid not fat (SNF), and ash in milk.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Veterinary Sciences
Matteo Cuccato, Sara Divari, Paola Sacchi, Flavia Girolami, Francesca Tiziana Cannizzo
Summary: This study applied MALDI-TOF mass spectrometry to analyze milk samples from cows with different SCC, and found significant differences in polypeptide/protein profiles between the two groups. Classification algorithms were used to classify new spectra into the two classes, supporting MALDI-TOF mass spectrometry profiling as a tool to detect mastitic milk samples.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Agriculture, Multidisciplinary
Bin Yang, Fang He, Cheng Huan, Renke Hu, Jianbo Li, Kangle Yi, Zhiwei Kong, Yang Luo
Summary: The impacts of high milk somatic cell count (SCC) on the protein composition of milk exosomes, milk fat globule membrane (MFGM), and whey are significant, especially in the case of high SCC where there are noticeable changes in the proteins of exosomes and MFGM. These findings provide guidance for the early detection of unhealthy mammary glands.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Veterinary Sciences
Charlotte Langhorne, Suman Das Gupta, Sara Horsman, Caitlin Wood, Benjamin J. Wood, Leslie Barker, Ania Deutscher, Rochelle Price, Michael R. Mcgowan, Mark Humphris, Shahab Ranjbar, Joerg Henning, Justine S. Gibson
Summary: This study retrospectively analyzed the mastitis-causing organisms isolated from bovine milk samples in Australia. The study identified temporal, geographical, and seasonal patterns of occurrence for these organisms and reported their in vitro susceptibility. The findings emphasized the importance of further research into specific risk factors to improve mastitis control and treatment.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Jinning Zhang, Wenlong Li, Yongjie Tang, Xueqin Liu, Hailiang Zhang, Yueling Zhou, Yachun Wang, Wei Xiao, Ying Yu
Summary: This study compared the effect of two commonly used SCC thresholds on distinguishing milk microbiota and host gene expression patterns. It found significant differences in microbial composition and gene expression between cows with SCC above and below 100,000 cells/mL, suggesting that 100,000 cells/mL is a more suitable cut-off value for subclinical mastitis diagnosis than 200,000 cells/mL.
Article
Infectious Diseases
Anne Bunge, Sonja Dreyer, Jan-Hendrik Paduch, Doris Klocke, Stefanie Leimbach, Nicole Wente, Julia Nitz, Volker Kromker
Summary: To optimise udder health at the herd level, it is necessary to identify incurable mastitis cases and provide adequate therapy and culling strategies. This study assessed the cell count and cell viability in DHI milk samples and found that the proportion of non-vital cells can enhance the prediction of whether antibiotic treatment of clinical mastitis is promising or not.
Review
Veterinary Sciences
Ana Ines Molineri, Cecilia Camussone, M. Virginia Zbrun, Guillermo Suarez Archilla, Mariana Cristiani, Veronica Neder, Luis Calvinho, Marcelo Signorini
Summary: Staphylococcus aureus shows increasing antimicrobial resistance over time, with penicillin, clindamycin, erythromycin, and gentamycin having the highest resistance rates. Africa, Asia, and Latin America display higher resistance rates, with no significant differences in resistance based on the clinical origin of the isolates.
PREVENTIVE VETERINARY MEDICINE
(2021)
Article
Microbiology
Umarani Brahma, Akash Suresh, Shweta Murthy, Vasundhra Bhandari, Paresh Sharma
Summary: This study isolated and characterized 80 clinical isolates of Staphylococcus aureus from mastitis-infected animals. The majority of isolates were found to be multidrug-resistant (MDR), with a significant proportion being methicillin-resistant S. aureus (MRSA). The isolates showed a wide range of molecular and phenotypic features, including different sequence types (STs) and biofilm production. The presence of the lukS gene was found to be associated with biofilm formation. The study highlights the importance of comprehensive surveillance of S. aureus and other mastitis-causing pathogens.
Article
Agriculture, Dairy & Animal Science
Giovanni Niero, Steffi Anna Thomas, Kassiani Mouratidou, Giulio Visentin, Massimo De Marchi, Mauro Penasa, Martino Cassandro
Summary: The present study evaluated the performance of the radial immunodiffusion (RID) technique for quantifying milk lactoferrin (LF) and investigated the sources of variation in LF concentration in individual milk samples. The RID method showed good repeatability and reproducibility, indicating its suitability as a consistent test for LF quantification. Milk LF was found to be positively associated with somatic cell score and negatively associated with lactose content. Lower LF concentration was observed in milk from healthy and susceptible cows compared to mastitic and chronic cows. Milk LF has the potential to be a useful biomarker for early mastitis detection.
ITALIAN JOURNAL OF ANIMAL SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
V Asselstine, J. F. Medrano, A. Canovas
Summary: This study used transcriptomics to study the functional aspect of mastitis resistance and found differential expression of mRNA isoforms generated via alternative splicing in mastitic and healthy samples. The study also identified the potential involvement of these mRNA isoforms in the immune system and mastitis.
Article
Biochemical Research Methods
Lei He, Bing Chen, Yu Hu, Boheng Hu, Ya Li, Xiaonan Yang
Summary: We have developed a highly integrated point-of-care testing device for immediate and accurate screening of bovine mastitis infection using somatic cell counting (SCC). The system includes a homemade cell-counting chamber and a miniature fluorescent microscope. By pre-embedding the chamber with acridine orange, SCC can be directly identified through microscopic imaging analysis, enabling quick and efficient diagnosis of bovine mastitis within 6 minutes. The system has a detection limit of 2.12 x 10(4) cells/mL and can differentiate diseased cows from healthy ones with an accuracy of 98.0%. It is user-friendly and low-cost, making it a potential tool for on-site diagnosis in resource-limited areas.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Agriculture, Dairy & Animal Science
Romero Kadran Rodrigues Vieira, Marcos Rodrigues, Patricia Kaline da Silva Santos, Natalia Bianca Caires Medeiros, Ebson Pereira Candido, Marilia Danyelle Nunes Rodrigues
Summary: This study evaluated the main risk factors for bovine mastitis in the southeastern region of Para, Brazilian Amazon. The results showed that irrigated pastures and specific breeds of cows were significant risk factors for mastitis occurrence.
TROPICAL ANIMAL HEALTH AND PRODUCTION
(2022)